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The map label for this gene is purD [H]

Identifier: 158424010

GI number: 158424010

Start: 2711991

End: 2713325

Strand: Direct

Name: purD [H]

Synonym: AZC_2386

Alternate gene names: 158424010

Gene position: 2711991-2713325 (Clockwise)

Preceding gene: 158424007

Following gene: 158424011

Centisome position: 50.5

GC content: 68.31

Gene sequence:

>1335_bases
ATGCGGCGGGCGCAATGCCCTACCCCTTGTTCACGGCCTCTCGACCCGATAGCCAAACCGAAACGGGGAAACAGTCCGAT
GAACGTGCTCCTGCTCGGTTCCGGTGGCCGCGAACATGCCCTTGCCTGGAAGCTCGCCCAGAGCCCGGACATGGGTCAAT
TCTATGCCCTGCCGGGCAACCCCGGCATTTCCGATTTCGCCGAGGCCATCGAGGGCATCGCTTTGTCCGCCCACGACACG
CTCGTGCGCTGGTGCAAGGACCATGCGATCGAACTCGTGATCGTCGGCCCCGAAGCACCGCTGGTGGCGGGTCTCGTGGA
CCGGCTGGCGGAAGCCGGCATCGCCGCCTTCGGCCCCACCGCCGCCGCCGCGCGCCTTGAGGGCTCCAAGCTCTTCACCA
AGGAACTGTGCCGCGCCAACGGCATTCCCACCGCCGCCTTCGCCCGCTTCACCAAGGCGGAAGCCGCCTGGGCCTATGTG
ACCGCCGAGGGCGCCCCCATCGTCATCAAGGCCGACGGCCTCATGGCCGGCAAGGGCGTGGTGGTGGCGCAGACGGTGGA
AGAAGCCATCTCCGCCATCGATCAGGTGTTCGCCGCCGGCGGGCAGGAAGTGCTGATCGAGGAATTCATGGAGGGCGAGG
AGGCGAGCCTCTTCTGCCTCGTGGACGGCGAGACCGTGGTGCCCTTCGGCAGCGCGCAGGACCACAAGCGCGCCTTCGAC
GGCGACCTCGGCCCCAACACCGGCGGCATGGGCGCCTATTCCCCCGCCCCCGTGCTGACGCCGGAGCTGGAAAAGCGGGC
CATCGACGAGATCGTCATCCCCACCGCCAAGGCCATGGCGGCGGCGGGCACGCCTTATCGCGGCGTGCTCTATGCAGGCC
TGATGCTCACCTCCACCGGTCCCAAGCTCGTGGAATACAACTGCCGCTTCGGCGACCCGGAATGCCAGGTGCTGATGCCC
CGCTATACCGGCGACCTCCTGGAGACGCTGAATGCCGTCGCCCACGGCCGGCTGTCGCAGGTGAAGGTCTCCTGGTCGGA
CGACGCCGCCATCACGGTGGTCTATGCCAGCCGCGGCTATCCCGGCGCCCACCAGACGGGCAGCGAGATCAAGGGGATCG
AGGCCGCCGCCGCACTGCCGGACGTGCTGGTCTTCCAGGCCGGCACCAAGAAGAACGGTGCCCGCCTGCTCTCCAACGGC
GGCCGCGTGCTGAACGTGACCGCCACCGGCAAGACGTTGAAGGATGCCCGCGACCGGGCTTATGAGGCCGCCGGCCTCAT
CGACTGGCCGGATGGCTTCCACCGCAACGACATCGGCTGGCGCGCCCTGCGCTGA

Upstream 100 bases:

>100_bases
AGACGGTCCATTCCGGCGCATTGGCGCGGGTTTCAGGCAGATCGGTCATCGAATCAGACGGCACCAGACAATCGAGGCAG
AAGCATAGCCGCTATCTCAT

Downstream 100 bases:

>100_bases
CCGCAGGCCGATTTTCGACTTCTGCAACGGCGGGACACCAGTCCCGCCGTTTTGCGTTGTGCGGGTGGTGGCGCGCCCGA
CGGCGTGCGACGCTGACCGA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRRAQCPTPCSRPLDPIAKPKRGNSPMNVLLLGSGGREHALAWKLAQSPDMGQFYALPGNPGISDFAEAIEGIALSAHDT
LVRWCKDHAIELVIVGPEAPLVAGLVDRLAEAGIAAFGPTAAAARLEGSKLFTKELCRANGIPTAAFARFTKAEAAWAYV
TAEGAPIVIKADGLMAGKGVVVAQTVEEAISAIDQVFAAGGQEVLIEEFMEGEEASLFCLVDGETVVPFGSAQDHKRAFD
GDLGPNTGGMGAYSPAPVLTPELEKRAIDEIVIPTAKAMAAAGTPYRGVLYAGLMLTSTGPKLVEYNCRFGDPECQVLMP
RYTGDLLETLNAVAHGRLSQVKVSWSDDAAITVVYASRGYPGAHQTGSEIKGIEAAAALPDVLVFQAGTKKNGARLLSNG
GRVLNVTATGKTLKDARDRAYEAAGLIDWPDGFHRNDIGWRALR

Sequences:

>Translated_444_residues
MRRAQCPTPCSRPLDPIAKPKRGNSPMNVLLLGSGGREHALAWKLAQSPDMGQFYALPGNPGISDFAEAIEGIALSAHDT
LVRWCKDHAIELVIVGPEAPLVAGLVDRLAEAGIAAFGPTAAAARLEGSKLFTKELCRANGIPTAAFARFTKAEAAWAYV
TAEGAPIVIKADGLMAGKGVVVAQTVEEAISAIDQVFAAGGQEVLIEEFMEGEEASLFCLVDGETVVPFGSAQDHKRAFD
GDLGPNTGGMGAYSPAPVLTPELEKRAIDEIVIPTAKAMAAAGTPYRGVLYAGLMLTSTGPKLVEYNCRFGDPECQVLMP
RYTGDLLETLNAVAHGRLSQVKVSWSDDAAITVVYASRGYPGAHQTGSEIKGIEAAAALPDVLVFQAGTKKNGARLLSNG
GRVLNVTATGKTLKDARDRAYEAAGLIDWPDGFHRNDIGWRALR
>Mature_444_residues
MRRAQCPTPCSRPLDPIAKPKRGNSPMNVLLLGSGGREHALAWKLAQSPDMGQFYALPGNPGISDFAEAIEGIALSAHDT
LVRWCKDHAIELVIVGPEAPLVAGLVDRLAEAGIAAFGPTAAAARLEGSKLFTKELCRANGIPTAAFARFTKAEAAWAYV
TAEGAPIVIKADGLMAGKGVVVAQTVEEAISAIDQVFAAGGQEVLIEEFMEGEEASLFCLVDGETVVPFGSAQDHKRAFD
GDLGPNTGGMGAYSPAPVLTPELEKRAIDEIVIPTAKAMAAAGTPYRGVLYAGLMLTSTGPKLVEYNCRFGDPECQVLMP
RYTGDLLETLNAVAHGRLSQVKVSWSDDAAITVVYASRGYPGAHQTGSEIKGIEAAAALPDVLVFQAGTKKNGARLLSNG
GRVLNVTATGKTLKDARDRAYEAAGLIDWPDGFHRNDIGWRALR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=47.4056603773585, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=47.4056603773585, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=47.4056603773585, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=47.4056603773585, Blast_Score=380, Evalue=1e-105,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=49.7674418604651, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=41.0377358490566, Blast_Score=285, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=40.8071748878924, Blast_Score=320, Evalue=4e-88,
Organism=Drosophila melanogaster, GI62471619, Length=433, Percent_Identity=42.0323325635104, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24582400, Length=433, Percent_Identity=42.0323325635104, Blast_Score=298, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46807; Mature: 46807

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAQCPTPCSRPLDPIAKPKRGNSPMNVLLLGSGGREHALAWKLAQSPDMGQFYALPGN
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCC
PGISDFAEAIEGIALSAHDTLVRWCKDHAIELVIVGPEAPLVAGLVDRLAEAGIAAFGPT
CCHHHHHHHHHCCEECHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCC
AAAARLEGSKLFTKELCRANGIPTAAFARFTKAEAAWAYVTAEGAPIVIKADGLMAGKGV
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCEEEEEECCEEECCCE
VVAQTVEEAISAIDQVFAAGGQEVLIEEFMEGEEASLFCLVDGETVVPFGSAQDHKRAFD
EEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCHHHHHCC
GDLGPNTGGMGAYSPAPVLTPELEKRAIDEIVIPTAKAMAAAGTPYRGVLYAGLMLTSTG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHEEEEEECCC
PKLVEYNCRFGDPECQVLMPRYTGDLLETLNAVAHGRLSQVKVSWSDDAAITVVYASRGY
CCEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCC
PGAHQTGSEIKGIEAAAALPDVLVFQAGTKKNGARLLSNGGRVLNVTATGKTLKDARDRA
CCCCCCCCHHCCCHHHHHCCCEEEEECCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHH
YEAAGLIDWPDGFHRNDIGWRALR
HHHCCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MRRAQCPTPCSRPLDPIAKPKRGNSPMNVLLLGSGGREHALAWKLAQSPDMGQFYALPGN
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCC
PGISDFAEAIEGIALSAHDTLVRWCKDHAIELVIVGPEAPLVAGLVDRLAEAGIAAFGPT
CCHHHHHHHHHCCEECHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCC
AAAARLEGSKLFTKELCRANGIPTAAFARFTKAEAAWAYVTAEGAPIVIKADGLMAGKGV
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCEEEEEECCEEECCCE
VVAQTVEEAISAIDQVFAAGGQEVLIEEFMEGEEASLFCLVDGETVVPFGSAQDHKRAFD
EEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCHHHHHCC
GDLGPNTGGMGAYSPAPVLTPELEKRAIDEIVIPTAKAMAAAGTPYRGVLYAGLMLTSTG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHEEEEEECCC
PKLVEYNCRFGDPECQVLMPRYTGDLLETLNAVAHGRLSQVKVSWSDDAAITVVYASRGY
CCEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCC
PGAHQTGSEIKGIEAAAALPDVLVFQAGTKKNGARLLSNGGRVLNVTATGKTLKDARDRA
CCCCCCCCHHCCCHHHHHCCCEEEEECCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHH
YEAAGLIDWPDGFHRNDIGWRALR
HHHCCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]