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The map label for this gene is cls [H]

Identifier: 158423980

GI number: 158423980

Start: 2684486

End: 2685937

Strand: Direct

Name: cls [H]

Synonym: AZC_2356

Alternate gene names: 158423980

Gene position: 2684486-2685937 (Clockwise)

Preceding gene: 158423968

Following gene: 158423983

Centisome position: 49.99

GC content: 65.15

Gene sequence:

>1452_bases
ATGCACGATTCGGCCGTTCTCGCCTGGCTGCTGTTCGCGGTCCATGCCAGCCTCCAGCTTTTCTTCATCGTTCGGGCGCT
GCTGCGCCCGCATCGCGAACCGTCCTCGCGCATCGCCTGGGTGCTGGTGATCGGCCTCGCGCCGGTGGTGGGCATCCTCG
CCTATGTGTTGTTCGGCGAGGTCAATCCGGGGCGCAGGCATGTTGCCCGCCTGAGGGAGGCATTCGAGAAACTGCCTCCG
GCACAGACCATCGCCGGGCCGTCCGCCGAGGCCGACCTGCCGGAACGCTATGTCCCCTTGTTTCGCGTCGGCCAGTCGGT
GAGCAATTATCTGCCGGTCGCCGGCAACAGGGCCCGCCTGATGGCCGATTCTGCCGCAGCGATCGCGTCGATGGTCGATG
ACATCGATGCCGCGCGCGAAAGCGTGCATGTGCTGTTCTATATCTGGCTGGCGGACGACAGCGGACTGAAGATCGCCGAA
GCCCTCAAGCGCGCGGCGGCACGCGGCGTCGTGTGCCGGGCGATGGCCGACGATCTCGGCTCGCGGCATCTCATCCGCTC
CTCCCATTGGGCCGACATGGCCAGCGCGGGAGTGCGCCTTGGGCGCGCGCTGCCGGTGGGCAACCTGCTGCTGCGGCCGT
TCAAGGGGCGGATCGATCTGCGCAATCACCGCAAGATCGTGGTGATCGACAATGCCATCACCTATTGCGGAAGCCAGAAT
TGCGCGGATGCCGCCTTCGCGCCCAAGGCCCGCTTTGCCCCATGGGTGGACGTCATGGCGCGGTTCGAGGGGCCGGTGGT
GCGCCAGAACCAGCACCTCTTCGCGAGCACCTGGATGGCGCAGGTGGAAGAGGATCTGTCTCCCCTATTTACGCCGACCG
TCTCAAGCGACGTGTCCAAAGCGGGGTTCGTGGCACAGGTCATCGGCTCCGGCCCCAGCGTGCGCTATTCGGCGATGCCC
GAAACCTTCGTGGCTCTGATGAATGCGGCGCGCCGTGAACTGGTGATCACGACGCCCTATTACGTGCCCGACGAACCGAT
CCAGGCCGCCCTGCGCGCAAGCGCGCGGCGTGGCGTCGCCACCACCATCGTCTTCCCGCGGCGGAACGATTCCTGGATCG
TCGCCGCCGCCAGCCGCAGCTATTATCAGGACCTGCTGGAGGCGGGTGTCGCGATCCGCGAATATGCTGGCGGCCTTCTT
CACGCCAAGACGCTGACGATCGATGGCGAAGTCACGCTGATCGGCTCGGCCAACATCGACCGCCGCAGTTTCGAACTGAA
TGCCGAAAACAACATCCTGCTCCATGATCCCGCCTTCACTCAGGCGCTGCGGGAACGTCAGCAGGTTTTCATGGATGCTG
CGGAGCCGGTCACTCTCGCCCATGTAGAGAGCTACGGCCTCTTGCGGCAGCTCTGGAATAACTCCGTCGCGATGCTCGGC
CCGATCCTGTGA

Upstream 100 bases:

>100_bases
GGCGCGCGCATCTCCTGCCTGTGCAGGCCGCGCACCACCGTGGGAACACCGCGCGGCGTGCTTTAATACGCCCTGTGATG
ATCCGGGATGTCTTCACTCC

Downstream 100 bases:

>100_bases
GCGCGGTCATGCGCGTCCGGCTCCGGTCAAAGTCATCCCCGTCGAAGGAATCTGCCGGGACCGCACGCGTCATCAAATGA
CGTCATGCGAGTGCGGGCCC

Product: phospholipase D

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MHDSAVLAWLLFAVHASLQLFFIVRALLRPHREPSSRIAWVLVIGLAPVVGILAYVLFGEVNPGRRHVARLREAFEKLPP
AQTIAGPSAEADLPERYVPLFRVGQSVSNYLPVAGNRARLMADSAAAIASMVDDIDAARESVHVLFYIWLADDSGLKIAE
ALKRAAARGVVCRAMADDLGSRHLIRSSHWADMASAGVRLGRALPVGNLLLRPFKGRIDLRNHRKIVVIDNAITYCGSQN
CADAAFAPKARFAPWVDVMARFEGPVVRQNQHLFASTWMAQVEEDLSPLFTPTVSSDVSKAGFVAQVIGSGPSVRYSAMP
ETFVALMNAARRELVITTPYYVPDEPIQAALRASARRGVATTIVFPRRNDSWIVAAASRSYYQDLLEAGVAIREYAGGLL
HAKTLTIDGEVTLIGSANIDRRSFELNAENNILLHDPAFTQALRERQQVFMDAAEPVTLAHVESYGLLRQLWNNSVAMLG
PIL

Sequences:

>Translated_483_residues
MHDSAVLAWLLFAVHASLQLFFIVRALLRPHREPSSRIAWVLVIGLAPVVGILAYVLFGEVNPGRRHVARLREAFEKLPP
AQTIAGPSAEADLPERYVPLFRVGQSVSNYLPVAGNRARLMADSAAAIASMVDDIDAARESVHVLFYIWLADDSGLKIAE
ALKRAAARGVVCRAMADDLGSRHLIRSSHWADMASAGVRLGRALPVGNLLLRPFKGRIDLRNHRKIVVIDNAITYCGSQN
CADAAFAPKARFAPWVDVMARFEGPVVRQNQHLFASTWMAQVEEDLSPLFTPTVSSDVSKAGFVAQVIGSGPSVRYSAMP
ETFVALMNAARRELVITTPYYVPDEPIQAALRASARRGVATTIVFPRRNDSWIVAAASRSYYQDLLEAGVAIREYAGGLL
HAKTLTIDGEVTLIGSANIDRRSFELNAENNILLHDPAFTQALRERQQVFMDAAEPVTLAHVESYGLLRQLWNNSVAMLG
PIL
>Mature_483_residues
MHDSAVLAWLLFAVHASLQLFFIVRALLRPHREPSSRIAWVLVIGLAPVVGILAYVLFGEVNPGRRHVARLREAFEKLPP
AQTIAGPSAEADLPERYVPLFRVGQSVSNYLPVAGNRARLMADSAAAIASMVDDIDAARESVHVLFYIWLADDSGLKIAE
ALKRAAARGVVCRAMADDLGSRHLIRSSHWADMASAGVRLGRALPVGNLLLRPFKGRIDLRNHRKIVVIDNAITYCGSQN
CADAAFAPKARFAPWVDVMARFEGPVVRQNQHLFASTWMAQVEEDLSPLFTPTVSSDVSKAGFVAQVIGSGPSVRYSAMP
ETFVALMNAARRELVITTPYYVPDEPIQAALRASARRGVATTIVFPRRNDSWIVAAASRSYYQDLLEAGVAIREYAGGLL
HAKTLTIDGEVTLIGSANIDRRSFELNAENNILLHDPAFTQALRERQQVFMDAAEPVTLAHVESYGLLRQLWNNSVAMLG
PIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=444, Percent_Identity=34.009009009009, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1787007, Length=343, Percent_Identity=28.2798833819242, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081826, Length=387, Percent_Identity=26.6149870801034, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 52945; Mature: 52945

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDSAVLAWLLFAVHASLQLFFIVRALLRPHREPSSRIAWVLVIGLAPVVGILAYVLFGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
VNPGRRHVARLREAFEKLPPAQTIAGPSAEADLPERYVPLFRVGQSVSNYLPVAGNRARL
CCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHE
MADSAAAIASMVDDIDAARESVHVLFYIWLADDSGLKIAEALKRAAARGVVCRAMADDLG
EHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SRHLIRSSHWADMASAGVRLGRALPVGNLLLRPFKGRIDLRNHRKIVVIDNAITYCGSQN
CCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEECCCCEEEEEECHHHHCCCCC
CADAAFAPKARFAPWVDVMARFEGPVVRQNQHLFASTWMAQVEEDLSPLFTPTVSSDVSK
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
AGFVAQVIGSGPSVRYSAMPETFVALMNAARRELVITTPYYVPDEPIQAALRASARRGVA
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCE
TTIVFPRRNDSWIVAAASRSYYQDLLEAGVAIREYAGGLLHAKTLTIDGEVTLIGSANID
EEEEEECCCCCEEEEECCHHHHHHHHHHCHHHHHHCCCEEEEEEEEECCEEEEEECCCCC
RRSFELNAENNILLHDPAFTQALRERQQVFMDAAEPVTLAHVESYGLLRQLWNNSVAMLG
CCEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHC
PIL
CCC
>Mature Secondary Structure
MHDSAVLAWLLFAVHASLQLFFIVRALLRPHREPSSRIAWVLVIGLAPVVGILAYVLFGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
VNPGRRHVARLREAFEKLPPAQTIAGPSAEADLPERYVPLFRVGQSVSNYLPVAGNRARL
CCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHE
MADSAAAIASMVDDIDAARESVHVLFYIWLADDSGLKIAEALKRAAARGVVCRAMADDLG
EHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SRHLIRSSHWADMASAGVRLGRALPVGNLLLRPFKGRIDLRNHRKIVVIDNAITYCGSQN
CCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEECCCCEEEEEECHHHHCCCCC
CADAAFAPKARFAPWVDVMARFEGPVVRQNQHLFASTWMAQVEEDLSPLFTPTVSSDVSK
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
AGFVAQVIGSGPSVRYSAMPETFVALMNAARRELVITTPYYVPDEPIQAALRASARRGVA
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCE
TTIVFPRRNDSWIVAAASRSYYQDLLEAGVAIREYAGGLLHAKTLTIDGEVTLIGSANID
EEEEEECCCCCEEEEECCHHHHHHHHHHCHHHHHHCCCEEEEEEEEECCEEEEEECCCCC
RRSFELNAENNILLHDPAFTQALRERQQVFMDAAEPVTLAHVESYGLLRQLWNNSVAMLG
CCEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHC
PIL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA