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The map label for this gene is rfaF [H]

Identifier: 158423875

GI number: 158423875

Start: 2573615

End: 2574751

Strand: Direct

Name: rfaF [H]

Synonym: AZC_2251

Alternate gene names: 158423875

Gene position: 2573615-2574751 (Clockwise)

Preceding gene: 158423874

Following gene: 158423876

Centisome position: 47.93

GC content: 71.94

Gene sequence:

>1137_bases
TTGCAAGGACGGGCATGGCTTGCCAAGAGCCCGCGTCCGCGCCAGATGCTACGGCAAGGCCGCGCCGCGCGCGGCGTGAT
CGCCCGGTCCGAGCGGGCGTGCCAGCGAGTGCCCATGCGTGCCATCGAAACCTATTCGCCGCTTCTGATCATCGGTCCCT
CCTGGGTCGGTGACATGGTGATGGCGGCGAGCCTCGTCGCGAGCCTCAGGGCGCAGGAGCCGGGCCGGCCCATCGATGTC
ATGGCCCCGCCGGCGGCGCTGCCCATCGCCCGGCTCATTCCTGGCGTGCGGCGGACCATCCCGCTGGGGCTCGGCCATGG
GCAGTTCGGCCTCATGGCCCGCTGGCGGGCGGGGCGGGCGCTCCGCAGCGAAGGGTACGGCAAGGCGATCATCCTGCCGC
GGGCCTTCAAGGCCGCGATCCCGCCCTTCGCTGCTGGCATTCCCGTGCGCATCGGCTATGCGGCCGAGGGGCGCAGCATC
CTGCTCACGGACGCGCGTTCGGATTCCCAGCGCAAGACGGCGCGGACCATTGACCGTTTCGTGGCCCTCGGCTCGCCGGA
AGGGGCGGCCCCCGCGTCCGAGCGGCCCGTCCTGGTGCTACCCGAGGAGGAGCGGCGCGCGGTCGCGGAGAAGTTCCCGC
TGGCCGGCGAGGGGCCCGTGATGGCGCTCTGCCCCGGCGCGGAATACGGGCCCGCCAAGCGCTGGCCCACCGCAAAGTTC
GCGACCCTCGCCGCGCAGGCCCATGCCGCCGGCTACCAGTTGCGGGTGCTGGGCGGCCCCAAGGATGCGCCGCTGGCGCA
GGAGATCGTCGCAAAGGCCGGCGTGCCGGTGGAGGATCTGACGGGGCGCACCTCGCTCATCGAGGCAGCCGGCATTCTGG
CGGCCGCCGACGTTGTAGTCTCCAACGATTCCGGGCTCATGCACGTGGCCGGCGCGCTCGATCGGCCGCTCGTGGTGCTC
TACGGATCGTCTTCCGAGAAGATGACGCCGCCCACAGGCCCCCGCTCGACCGTCGTGTCTCACGACCTGCCCTGCCGTCC
CTGCTTCAAGCGGGAATGCCCGCTCGGGACGCTCGCCTGTTTCGAGGCCATCAGCCCGCAGGAAGTGCTGGCCGCCGCCA
TGGCGGTGCAGGGATGA

Upstream 100 bases:

>100_bases
GCCGATCAAGGCCGGCGTCGAGAAGACCCTGCGTACCGGCGCCTGATCGCCGTACGTCGAGACTGCCATTCGAGAGCGCC
CTTCGGGGCGCTCTTTTCGT

Downstream 100 bases:

>100_bases
GCGTCCGGCTGCCATGAAGGTTCTGGTCATCAAGCTCTCCTCGCTGGGCGATGTGGTGCACACCTTCCCGGCGGTCACGG
ACGCGGCTCGCCGCCTGCCC

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MQGRAWLAKSPRPRQMLRQGRAARGVIARSERACQRVPMRAIETYSPLLIIGPSWVGDMVMAASLVASLRAQEPGRPIDV
MAPPAALPIARLIPGVRRTIPLGLGHGQFGLMARWRAGRALRSEGYGKAIILPRAFKAAIPPFAAGIPVRIGYAAEGRSI
LLTDARSDSQRKTARTIDRFVALGSPEGAAPASERPVLVLPEEERRAVAEKFPLAGEGPVMALCPGAEYGPAKRWPTAKF
ATLAAQAHAAGYQLRVLGGPKDAPLAQEIVAKAGVPVEDLTGRTSLIEAAGILAAADVVVSNDSGLMHVAGALDRPLVVL
YGSSSEKMTPPTGPRSTVVSHDLPCRPCFKRECPLGTLACFEAISPQEVLAAAMAVQG

Sequences:

>Translated_378_residues
MQGRAWLAKSPRPRQMLRQGRAARGVIARSERACQRVPMRAIETYSPLLIIGPSWVGDMVMAASLVASLRAQEPGRPIDV
MAPPAALPIARLIPGVRRTIPLGLGHGQFGLMARWRAGRALRSEGYGKAIILPRAFKAAIPPFAAGIPVRIGYAAEGRSI
LLTDARSDSQRKTARTIDRFVALGSPEGAAPASERPVLVLPEEERRAVAEKFPLAGEGPVMALCPGAEYGPAKRWPTAKF
ATLAAQAHAAGYQLRVLGGPKDAPLAQEIVAKAGVPVEDLTGRTSLIEAAGILAAADVVVSNDSGLMHVAGALDRPLVVL
YGSSSEKMTPPTGPRSTVVSHDLPCRPCFKRECPLGTLACFEAISPQEVLAAAMAVQG
>Mature_378_residues
MQGRAWLAKSPRPRQMLRQGRAARGVIARSERACQRVPMRAIETYSPLLIIGPSWVGDMVMAASLVASLRAQEPGRPIDV
MAPPAALPIARLIPGVRRTIPLGLGHGQFGLMARWRAGRALRSEGYGKAIILPRAFKAAIPPFAAGIPVRIGYAAEGRSI
LLTDARSDSQRKTARTIDRFVALGSPEGAAPASERPVLVLPEEERRAVAEKFPLAGEGPVMALCPGAEYGPAKRWPTAKF
ATLAAQAHAAGYQLRVLGGPKDAPLAQEIVAKAGVPVEDLTGRTSLIEAAGILAAADVVVSNDSGLMHVAGALDRPLVVL
YGSSSEKMTPPTGPRSTVVSHDLPCRPCFKRECPLGTLACFEAISPQEVLAAAMAVQG

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=340, Percent_Identity=39.1176470588235, Blast_Score=234, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 39893; Mature: 39893

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGRAWLAKSPRPRQMLRQGRAARGVIARSERACQRVPMRAIETYSPLLIIGPSWVGDMV
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCHHHHHHH
MAASLVASLRAQEPGRPIDVMAPPAALPIARLIPGVRRTIPLGLGHGQFGLMARWRAGRA
HHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHCCHHHHHEEECCCCCCHHHHHHHHHCHH
LRSEGYGKAIILPRAFKAAIPPFAAGIPVRIGYAAEGRSILLTDARSDSQRKTARTIDRF
HHHCCCCCEEEECHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
VALGSPEGAAPASERPVLVLPEEERRAVAEKFPLAGEGPVMALCPGAEYGPAKRWPTAKF
HHCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHH
ATLAAQAHAAGYQLRVLGGPKDAPLAQEIVAKAGVPVEDLTGRTSLIEAAGILAAADVVV
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHEEEEEEE
SNDSGLMHVAGALDRPLVVLYGSSSEKMTPPTGPRSTVVSHDLPCRPCFKRECPLGTLAC
ECCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHH
FEAISPQEVLAAAMAVQG
HHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQGRAWLAKSPRPRQMLRQGRAARGVIARSERACQRVPMRAIETYSPLLIIGPSWVGDMV
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCHHHHHHH
MAASLVASLRAQEPGRPIDVMAPPAALPIARLIPGVRRTIPLGLGHGQFGLMARWRAGRA
HHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHCCHHHHHEEECCCCCCHHHHHHHHHCHH
LRSEGYGKAIILPRAFKAAIPPFAAGIPVRIGYAAEGRSILLTDARSDSQRKTARTIDRF
HHHCCCCCEEEECHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
VALGSPEGAAPASERPVLVLPEEERRAVAEKFPLAGEGPVMALCPGAEYGPAKRWPTAKF
HHCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHH
ATLAAQAHAAGYQLRVLGGPKDAPLAQEIVAKAGVPVEDLTGRTSLIEAAGILAAADVVV
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHEEEEEEE
SNDSGLMHVAGALDRPLVVLYGSSSEKMTPPTGPRSTVVSHDLPCRPCFKRECPLGTLAC
ECCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHH
FEAISPQEVLAAAMAVQG
HHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]