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The map label for this gene is lysE [H]

Identifier: 158423692

GI number: 158423692

Start: 2381455

End: 2382093

Strand: Reverse

Name: lysE [H]

Synonym: AZC_2068

Alternate gene names: 158423692

Gene position: 2382093-2381455 (Counterclockwise)

Preceding gene: 158423693

Following gene: 158423682

Centisome position: 44.36

GC content: 69.33

Gene sequence:

>639_bases
ATGGACCCGGTTTCGGCGGTGATCGCCTTTTCCCTCGCGGCCGCACTGCTGGTCATCACGCCCGGCCTCGACACGGCGCT
CGTGCTCCGCACCGCTGCCGTGGAAGGGCCGCGCCCGGCCTTGCAGGCGGGTGCGGGCGTCTCGTCAGGCGTGCTGCTCT
GGGGACTGATCGCCTCGCTGGGCCTCGGGGCGCTGCTGGCGGTGTCGGAAACCGCCTATATGGTGCTCAAGGTCGTCGGC
AGCCTGTATCTGCTGTGGCTCGGCGGCCGCATGCTTCTGGCAGCACTACGCCCCGACGGTGGTGCCGGAGCGGCGGTGGT
CGTACCCCGTAACGCCTCAGCCGGCAACTGGTTCTGGCGCGGCCTGATGACCAATCTGCTGAATCCGAAGGTCGGCATCT
TCTACGTGACGTTCCTGCCCCAGTTCGTGCCGCCGGACATGCCGGTGGTAGCCTTCAGCTTCGGCCTCGCTGCCATCCAT
GTGGCGATGGGCATGAGCTGGTTCGCGCTGCTGGTCGGCGCAACACGTCCCTTCGCCCGCGTGCTCCAGAGCCCCACCGT
GCGCCGGGTGCTCGACGGGCTCACGGGGTGCGTGCTGGTCGCCTTCGGCCTGCGCCTCGCGCTGGAACGCCGGGGCTAA

Upstream 100 bases:

>100_bases
GATCCACGTGCCCGGCGGCTATTCAGATCCGAACGGCCCGAAGCGGCCGGAGGAACAGGGGGCGGCCGCAGGTCTTCGCG
TCGCCCCGCGCGGAGGCGCC

Downstream 100 bases:

>100_bases
TCCTCCGGCCGCGCCGCAACCCGCCGGTCGGCGAGCACGTTGCGGATGGCGAAATTGGAATTGATGCGCAGCACGCCGGG
AAGAACGGACAGGATCTCCT

Product: efflux protein

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MDPVSAVIAFSLAAALLVITPGLDTALVLRTAAVEGPRPALQAGAGVSSGVLLWGLIASLGLGALLAVSETAYMVLKVVG
SLYLLWLGGRMLLAALRPDGGAGAAVVVPRNASAGNWFWRGLMTNLLNPKVGIFYVTFLPQFVPPDMPVVAFSFGLAAIH
VAMGMSWFALLVGATRPFARVLQSPTVRRVLDGLTGCVLVAFGLRLALERRG

Sequences:

>Translated_212_residues
MDPVSAVIAFSLAAALLVITPGLDTALVLRTAAVEGPRPALQAGAGVSSGVLLWGLIASLGLGALLAVSETAYMVLKVVG
SLYLLWLGGRMLLAALRPDGGAGAAVVVPRNASAGNWFWRGLMTNLLNPKVGIFYVTFLPQFVPPDMPVVAFSFGLAAIH
VAMGMSWFALLVGATRPFARVLQSPTVRRVLDGLTGCVLVAFGLRLALERRG
>Mature_212_residues
MDPVSAVIAFSLAAALLVITPGLDTALVLRTAAVEGPRPALQAGAGVSSGVLLWGLIASLGLGALLAVSETAYMVLKVVG
SLYLLWLGGRMLLAALRPDGGAGAAVVVPRNASAGNWFWRGLMTNLLNPKVGIFYVTFLPQFVPPDMPVVAFSFGLAAIH
VAMGMSWFALLVGATRPFARVLQSPTVRRVLDGLTGCVLVAFGLRLALERRG

Specific function: Conducts The Efflux Of Threonine. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=200, Percent_Identity=29.5, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788099, Length=196, Percent_Identity=27.0408163265306, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI48994979, Length=140, Percent_Identity=32.1428571428571, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786522, Length=218, Percent_Identity=27.5229357798165, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22080; Mature: 22080

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPVSAVIAFSLAAALLVITPGLDTALVLRTAAVEGPRPALQAGAGVSSGVLLWGLIASL
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHH
GLGALLAVSETAYMVLKVVGSLYLLWLGGRMLLAALRPDGGAGAAVVVPRNASAGNWFWR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCHHHHH
GLMTNLLNPKVGIFYVTFLPQFVPPDMPVVAFSFGLAAIHVAMGMSWFALLVGATRPFAR
HHHHHHCCCCHHEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VLQSPTVRRVLDGLTGCVLVAFGLRLALERRG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDPVSAVIAFSLAAALLVITPGLDTALVLRTAAVEGPRPALQAGAGVSSGVLLWGLIASL
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHH
GLGALLAVSETAYMVLKVVGSLYLLWLGGRMLLAALRPDGGAGAAVVVPRNASAGNWFWR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCHHHHH
GLMTNLLNPKVGIFYVTFLPQFVPPDMPVVAFSFGLAAIHVAMGMSWFALLVGATRPFAR
HHHHHHCCCCHHEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VLQSPTVRRVLDGLTGCVLVAFGLRLALERRG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9308178 [H]