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The map label for this gene is gsiB [H]

Identifier: 158423653

GI number: 158423653

Start: 2340068

End: 2341684

Strand: Reverse

Name: gsiB [H]

Synonym: AZC_2029

Alternate gene names: 158423653

Gene position: 2341684-2340068 (Counterclockwise)

Preceding gene: 158423654

Following gene: 158423652

Centisome position: 43.61

GC content: 63.76

Gene sequence:

>1617_bases
GTGACCGACAAGACCGTTTCCAACTGGACCCGTGCCGACGACGCCATGGTTGAGAGCGCCATCCGCCGGGGCGCTTCGCG
CCGCGATCTCATGCGCATGCTGCTGGCCGGCGGCATCGCCTCCACCGCTTTCGGCTCTGGCCTCATTGCCCGTGCCACGG
ATGCCGTGGCGCAGACGCCGGTCTCCGGCGGCAATCTGAAGGCTGCCGGCTGGTCCGCCTCCACGGCCGACACGCTCGAT
CCGGCGAAAGCCTCGCTGTCGACCGACTATGTGCGCTGCTGCGCCTTCTACAACCGCCTGACCTTCATCGACAAAGGCGG
CGCCGTGCGCATGGAACTGGCCGAGAGCGTCGAGAGCGGCGACGCCAAGGTCTGGACCGTCAAGCTGCGCAAGGGCGTCA
CGTTCCACGACGGCAAGACGCTGACCTCGGCCGACGTGGTCTATTCACTGAAGCGTCACCTCGATCCGGCGGTCGGCTCG
AAGGTGAACTCCATCGCCAAGCAGATGAGCGAGATCAAGGCCGTGGACCCGTCCACGGTGGAGATCACTCTCGCCAATCC
AAATGGCGACCTGCCCACCATCCTCGGCATGCATCATTTCATGATCATTGCCGAAGGCACCACCACCTTCGCCAAGGCCA
ACGGCACCGGAGCCTTCGTCTGCGAGACCTTCGAGCCCGGCGTGCGCTCCATCGGACTGAAGAACAAGAACTACTGGAAG
AGCGGCTATCCGCACCTCGATTCCTTCGAGTTCTTTGCCATTTCGGACGATGCGGCGCGCGTGAATGCGCTGCTGTCCGG
CGACATCCAGCTCGCCGCCGCGATCAATCCGCGCTCCATGCGCCTGGTGGAAAGCCAGCCCGGCGTGCTGCTGTCGCGCA
CCACCTCCGGCAACTACACCGACCTCAACATGCGCCTCGACATGTCGCCCGGCGACAAGAAGGACTTCGTGGAGGGCGTG
AAATATCTGCTCAATCGCGAGCAGATCCAGAAGTCCGCCCTGCGGGGCCTGGCGGAGATCGCCAACGACCAGCCGATCCC
CCCCTCCAACATGTATTACAATGCCGAACTGAAACCGAAGCCCTTCGATCCGCAGAAGGCGAAGGCGCTGTTCCAGAAGG
CCGGCGTGCTCGGCCAGCCCATTCCCGTGGTGACGTCCGAAGCGGCGAACTCGGCCATCGACATGGCCATGGTGCTGCAG
CAGGCCGGCCAGGGCATCGGCATGAACTTCGACATCAAGCGCGTGCCGTCGGACGGCTACTGGTCCAACTACTGGCTGAA
GGCGCCCATGCACTTCGGCAACATCAATCCGCGCCCCACGCCCGACATCCTCTTCTCGCTGCTCTATGCCTCAAGCGCCC
CGTGGAACGAGAGCCAGTACAAGTCGGAGAAGTTCGACCGGCTGCTGCTTGAGGCACGCGGCTCGCTCGATCAGGCCAGG
CGCAAGCAGATCTATGGCGAAATGCAGGTGATGATTGCGGAGGAGGCGGGCACGGCCATCCCGGTCTTCATCTCCAATGT
GGACGCCCATTCCTCCAAGCTGAAGGGCCTCGAGCCCAATCCGCTGGGTGGCATGATGGGCTACGCCTTCGCCGAGTACG
TCTGGCTCGCATCCTGA

Upstream 100 bases:

>100_bases
CGCGGCACGCAGCGTAACGGGTGCGTTCGAGAAGACGGCCGGGGAGCTGACCTTCCCTCCCCGCCGGGCCTGTCAACGAG
AGACAAAAAAGGGGATCCTC

Downstream 100 bases:

>100_bases
CCGCAAAGCCGGCAGGCCGCTTCAGGCCTCGCCGGGGCCGCCCGGTGATCCGTCATCCTCGCGCGGGAGCGCCAATGAAC
AGCTATGTCCTGTCCCTTCT

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTDKTVSNWTRADDAMVESAIRRGASRRDLMRMLLAGGIASTAFGSGLIARATDAVAQTPVSGGNLKAAGWSASTADTLD
PAKASLSTDYVRCCAFYNRLTFIDKGGAVRMELAESVESGDAKVWTVKLRKGVTFHDGKTLTSADVVYSLKRHLDPAVGS
KVNSIAKQMSEIKAVDPSTVEITLANPNGDLPTILGMHHFMIIAEGTTTFAKANGTGAFVCETFEPGVRSIGLKNKNYWK
SGYPHLDSFEFFAISDDAARVNALLSGDIQLAAAINPRSMRLVESQPGVLLSRTTSGNYTDLNMRLDMSPGDKKDFVEGV
KYLLNREQIQKSALRGLAEIANDQPIPPSNMYYNAELKPKPFDPQKAKALFQKAGVLGQPIPVVTSEAANSAIDMAMVLQ
QAGQGIGMNFDIKRVPSDGYWSNYWLKAPMHFGNINPRPTPDILFSLLYASSAPWNESQYKSEKFDRLLLEARGSLDQAR
RKQIYGEMQVMIAEEAGTAIPVFISNVDAHSSKLKGLEPNPLGGMMGYAFAEYVWLAS

Sequences:

>Translated_538_residues
MTDKTVSNWTRADDAMVESAIRRGASRRDLMRMLLAGGIASTAFGSGLIARATDAVAQTPVSGGNLKAAGWSASTADTLD
PAKASLSTDYVRCCAFYNRLTFIDKGGAVRMELAESVESGDAKVWTVKLRKGVTFHDGKTLTSADVVYSLKRHLDPAVGS
KVNSIAKQMSEIKAVDPSTVEITLANPNGDLPTILGMHHFMIIAEGTTTFAKANGTGAFVCETFEPGVRSIGLKNKNYWK
SGYPHLDSFEFFAISDDAARVNALLSGDIQLAAAINPRSMRLVESQPGVLLSRTTSGNYTDLNMRLDMSPGDKKDFVEGV
KYLLNREQIQKSALRGLAEIANDQPIPPSNMYYNAELKPKPFDPQKAKALFQKAGVLGQPIPVVTSEAANSAIDMAMVLQ
QAGQGIGMNFDIKRVPSDGYWSNYWLKAPMHFGNINPRPTPDILFSLLYASSAPWNESQYKSEKFDRLLLEARGSLDQAR
RKQIYGEMQVMIAEEAGTAIPVFISNVDAHSSKLKGLEPNPLGGMMGYAFAEYVWLAS
>Mature_537_residues
TDKTVSNWTRADDAMVESAIRRGASRRDLMRMLLAGGIASTAFGSGLIARATDAVAQTPVSGGNLKAAGWSASTADTLDP
AKASLSTDYVRCCAFYNRLTFIDKGGAVRMELAESVESGDAKVWTVKLRKGVTFHDGKTLTSADVVYSLKRHLDPAVGSK
VNSIAKQMSEIKAVDPSTVEITLANPNGDLPTILGMHHFMIIAEGTTTFAKANGTGAFVCETFEPGVRSIGLKNKNYWKS
GYPHLDSFEFFAISDDAARVNALLSGDIQLAAAINPRSMRLVESQPGVLLSRTTSGNYTDLNMRLDMSPGDKKDFVEGVK
YLLNREQIQKSALRGLAEIANDQPIPPSNMYYNAELKPKPFDPQKAKALFQKAGVLGQPIPVVTSEAANSAIDMAMVLQQ
AGQGIGMNFDIKRVPSDGYWSNYWLKAPMHFGNINPRPTPDILFSLLYASSAPWNESQYKSEKFDRLLLEARGSLDQARR
KQIYGEMQVMIAEEAGTAIPVFISNVDAHSSKLKGLEPNPLGGMMGYAFAEYVWLAS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=530, Percent_Identity=26.9811320754717, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789966, Length=525, Percent_Identity=25.1428571428571, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789887, Length=434, Percent_Identity=24.6543778801843, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787762, Length=485, Percent_Identity=23.9175257731959, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787495, Length=545, Percent_Identity=21.8348623853211, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789397, Length=536, Percent_Identity=23.8805970149254, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787551, Length=535, Percent_Identity=22.2429906542056, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58477; Mature: 58346

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTVSNWTRADDAMVESAIRRGASRRDLMRMLLAGGIASTAFGSGLIARATDAVAQTP
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCC
VSGGNLKAAGWSASTADTLDPAKASLSTDYVRCCAFYNRLTFIDKGGAVRMELAESVESG
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCEEEHHHHHHHCCC
DAKVWTVKLRKGVTFHDGKTLTSADVVYSLKRHLDPAVGSKVNSIAKQMSEIKAVDPSTV
CCEEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEE
EITLANPNGDLPTILGMHHFMIIAEGTTTFAKANGTGAFVCETFEPGVRSIGLKNKNYWK
EEEEECCCCCCCHHHCCEEEEEEECCCCEEEECCCCEEEEEECCCCCHHHCCCCCCCCCC
SGYPHLDSFEFFAISDDAARVNALLSGDIQLAAAINPRSMRLVESQPGVLLSRTTSGNYT
CCCCCCCCEEEEEECCCHHHHHHEEECCEEEEEEECCCCEEEEECCCCEEEEECCCCCEE
DLNMRLDMSPGDKKDFVEGVKYLLNREQIQKSALRGLAEIANDQPIPPSNMYYNAELKPK
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCC
PFDPQKAKALFQKAGVLGQPIPVVTSEAANSAIDMAMVLQQAGQGIGMNFDIKRVPSDGY
CCCHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
WSNYWLKAPMHFGNINPRPTPDILFSLLYASSAPWNESQYKSEKFDRLLLEARGSLDQAR
CCCEEEECCEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
RKQIYGEMQVMIAEEAGTAIPVFISNVDAHSSKLKGLEPNPLGGMMGYAFAEYVWLAS
HHHHHCCEEEEEECCCCCEEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHEECC
>Mature Secondary Structure 
TDKTVSNWTRADDAMVESAIRRGASRRDLMRMLLAGGIASTAFGSGLIARATDAVAQTP
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCC
VSGGNLKAAGWSASTADTLDPAKASLSTDYVRCCAFYNRLTFIDKGGAVRMELAESVESG
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCEEEHHHHHHHCCC
DAKVWTVKLRKGVTFHDGKTLTSADVVYSLKRHLDPAVGSKVNSIAKQMSEIKAVDPSTV
CCEEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEE
EITLANPNGDLPTILGMHHFMIIAEGTTTFAKANGTGAFVCETFEPGVRSIGLKNKNYWK
EEEEECCCCCCCHHHCCEEEEEEECCCCEEEECCCCEEEEEECCCCCHHHCCCCCCCCCC
SGYPHLDSFEFFAISDDAARVNALLSGDIQLAAAINPRSMRLVESQPGVLLSRTTSGNYT
CCCCCCCCEEEEEECCCHHHHHHEEECCEEEEEEECCCCEEEEECCCCEEEEECCCCCEE
DLNMRLDMSPGDKKDFVEGVKYLLNREQIQKSALRGLAEIANDQPIPPSNMYYNAELKPK
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCC
PFDPQKAKALFQKAGVLGQPIPVVTSEAANSAIDMAMVLQQAGQGIGMNFDIKRVPSDGY
CCCHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
WSNYWLKAPMHFGNINPRPTPDILFSLLYASSAPWNESQYKSEKFDRLLLEARGSLDQAR
CCCEEEECCEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
RKQIYGEMQVMIAEEAGTAIPVFISNVDAHSSKLKGLEPNPLGGMMGYAFAEYVWLAS
HHHHHCCEEEEEECCCCCEEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA