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The map label for this gene is lysR [H]

Identifier: 158423519

GI number: 158423519

Start: 2171305

End: 2172207

Strand: Reverse

Name: lysR [H]

Synonym: AZC_1895

Alternate gene names: 158423519

Gene position: 2172207-2171305 (Counterclockwise)

Preceding gene: 158423522

Following gene: 158423518

Centisome position: 40.45

GC content: 67.0

Gene sequence:

>903_bases
ATGCAGGTTCCCTTACGGGCCATTGGTGTCTTCCACGCGGTTGCCCGTACGGGCAGCGTGACCCGTGCTGCCGATGAACT
GGGCGTCACGCCCTCCGCCGTCAGCCAGCAGGTGCAGGCGCTGGAGATCAGCCTCGGTACGGCCCTCATCGGCAAGGCCG
GGCGCAACGTCGTGCTGACCGAAGCCGGCGAGCGCTATTTCGAAATGATCCGTGGCGAGGTGGAGCGGATCACCGAGGCG
ACGCAGCGCATCCGCGGCTTCCGGTCTGTCACCACCCTCACAGTGCGGGCGACGCCGAGCCTTGCCACCAAATGGCTGCT
GCCGCGTCTCGCCGGCTTCATCGACGCCCATCCCGAGATCGAGCTGCGCCTCGACGGCACCAACGAGCCGACCGCCTTCC
AGAAGGAGAATGTGGATGTGGAAATCCGCCACGGCACGGGCCAATGGCCGGGCCTCGTTGCGGAAGGGCTGGTGGAAGAG
CGTTTCCTCCCCGTCTGCTCGCCCGCCCTCGCCGCTGCGGGCAGCCTCGCGGCGGAGGATTTGCCCCGCTTCCGGCTCAT
CCATTCGGTGAAATCGCAGGTTCAATGGCCCTATTGGTTCACCTGCGCCGGCGTAACGCCCGCTGAGCGGCTGCGCCGCC
TGTTCTTCGACCGCACCCACATGGCCATCGACGCCGCCGTGGGCGGCCTCGGCATCGCGCTGGAGAGCGATCTCATGATG
TGGGCGGAGAAGCACGATGGACGCCTCATCTGCCCCATTGCGGAGCCGCCCCGGATCACTCTTGTCACCCAATGGGCGAC
CTGCCCGCCCGATCACCTGCGCCACAGCAAGGTGAAGCTGTTCTTCGACTGGCTGCGTCGCGAGCGGGACAACTGGCTGG
CCACCCGCGACGCCTCACTTTAG

Upstream 100 bases:

>100_bases
GTCCGGCGGCATTCTTGATGGCACGACAGTAGGAAGCTTGTTCGTGAAGAACAATTCAGATTGCTTGCACTTCTCTTTAG
CCTGACTTAATCATTGACGC

Downstream 100 bases:

>100_bases
CTTGCCTAACAGATCCGTTCAGATTTCTGGATAGTGCTTCACAAATCCCGCTTCTAGCCTTGCATCCATCGCGGTCGCGC
TTCGGCGCAGATCATGGACC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MQVPLRAIGVFHAVARTGSVTRAADELGVTPSAVSQQVQALEISLGTALIGKAGRNVVLTEAGERYFEMIRGEVERITEA
TQRIRGFRSVTTLTVRATPSLATKWLLPRLAGFIDAHPEIELRLDGTNEPTAFQKENVDVEIRHGTGQWPGLVAEGLVEE
RFLPVCSPALAAAGSLAAEDLPRFRLIHSVKSQVQWPYWFTCAGVTPAERLRRLFFDRTHMAIDAAVGGLGIALESDLMM
WAEKHDGRLICPIAEPPRITLVTQWATCPPDHLRHSKVKLFFDWLRRERDNWLATRDASL

Sequences:

>Translated_300_residues
MQVPLRAIGVFHAVARTGSVTRAADELGVTPSAVSQQVQALEISLGTALIGKAGRNVVLTEAGERYFEMIRGEVERITEA
TQRIRGFRSVTTLTVRATPSLATKWLLPRLAGFIDAHPEIELRLDGTNEPTAFQKENVDVEIRHGTGQWPGLVAEGLVEE
RFLPVCSPALAAAGSLAAEDLPRFRLIHSVKSQVQWPYWFTCAGVTPAERLRRLFFDRTHMAIDAAVGGLGIALESDLMM
WAEKHDGRLICPIAEPPRITLVTQWATCPPDHLRHSKVKLFFDWLRRERDNWLATRDASL
>Mature_300_residues
MQVPLRAIGVFHAVARTGSVTRAADELGVTPSAVSQQVQALEISLGTALIGKAGRNVVLTEAGERYFEMIRGEVERITEA
TQRIRGFRSVTTLTVRATPSLATKWLLPRLAGFIDAHPEIELRLDGTNEPTAFQKENVDVEIRHGTGQWPGLVAEGLVEE
RFLPVCSPALAAAGSLAAEDLPRFRLIHSVKSQVQWPYWFTCAGVTPAERLRRLFFDRTHMAIDAAVGGLGIALESDLMM
WAEKHDGRLICPIAEPPRITLVTQWATCPPDHLRHSKVKLFFDWLRRERDNWLATRDASL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=35.8885017421603, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=34.0277777777778, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=27.3037542662116, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.0547945205479, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786401, Length=170, Percent_Identity=31.1764705882353, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787128, Length=145, Percent_Identity=30.3448275862069, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87081978, Length=170, Percent_Identity=30, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=31.4814814814815, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787589, Length=146, Percent_Identity=32.1917808219178, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33332; Mature: 33332

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVPLRAIGVFHAVARTGSVTRAADELGVTPSAVSQQVQALEISLGTALIGKAGRNVVLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
EAGERYFEMIRGEVERITEATQRIRGFRSVTTLTVRATPSLATKWLLPRLAGFIDAHPEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHCCCCCE
ELRLDGTNEPTAFQKENVDVEIRHGTGQWPGLVAEGLVEERFLPVCSPALAAAGSLAAED
EEEECCCCCCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCHHHHH
LPRFRLIHSVKSQVQWPYWFTCAGVTPAERLRRLFFDRTHMAIDAAVGGLGIALESDLMM
CHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
WAEKHDGRLICPIAEPPRITLVTQWATCPPDHLRHSKVKLFFDWLRRERDNWLATRDASL
HHCCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
>Mature Secondary Structure
MQVPLRAIGVFHAVARTGSVTRAADELGVTPSAVSQQVQALEISLGTALIGKAGRNVVLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
EAGERYFEMIRGEVERITEATQRIRGFRSVTTLTVRATPSLATKWLLPRLAGFIDAHPEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHCCCCCE
ELRLDGTNEPTAFQKENVDVEIRHGTGQWPGLVAEGLVEERFLPVCSPALAAAGSLAAED
EEEECCCCCCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCHHHHH
LPRFRLIHSVKSQVQWPYWFTCAGVTPAERLRRLFFDRTHMAIDAAVGGLGIALESDLMM
CHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
WAEKHDGRLICPIAEPPRITLVTQWATCPPDHLRHSKVKLFFDWLRRERDNWLATRDASL
HHCCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]