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The map label for this gene is yisK [H]

Identifier: 158423255

GI number: 158423255

Start: 1862467

End: 1863507

Strand: Direct

Name: yisK [H]

Synonym: AZC_1631

Alternate gene names: 158423255

Gene position: 1862467-1863507 (Clockwise)

Preceding gene: 158423253

Following gene: 158423260

Centisome position: 34.68

GC content: 64.65

Gene sequence:

>1041_bases
TTGATGGATACGGGACGCAGGTCGTTCATGGTTTCTGTCGCGGCTGCCGCAGCGGCAGCGGCGCCTTTGATGGTCGGCCC
AACACATGAGGCTGTGGCGCAGACCAATGATCCGGGCGGCGCGCTGAAACTATTGAGCTTCCGCTTGCGGGACGGGGCGC
CGCCGCGCGTCGGCGTCGCGCTTGCGGATGGCACGTTGGTTGATCTCACGGCGGAGGCCGCGCGCAGCGGCAAGACGCTG
TCCTTCAAGCCGGACGATATGGTCTCGCTCATCGCGGCGGGCGACACCGGGCTGGCGGAGGTACGCGAACTGGTGGCGCA
GGCGGCGCCGGGGCGCGTGAAGCTCACGGAGATCATCTATCTGCCGCCGATCCCCAATCCCCAGCGCAACATCTATGCGG
TGGGCTGGAATTATCTGGAGCACTTTGCCGAGGGGCAGGCGGTGCGGATGACGGACACCAAATATCCCGAACATCCGGTC
TTCTTCACCAAGGGCACACATTCCATCAACGGCCCGTTCGATCCCATTCCCTTCGACCCCAAGGTTTCCACCCAGATCGA
CTGGGAGGGGGAACTCGCCATCGTCATCGGCAAGCGAGGGCGCAACATTCCCGAGGCCGAGGCTATGGACTATGTCTTCG
GCTATTCCGTTATCAACGACACCACCGCCCGCGATGTGCAGAATGCGCGCCATGGCGGCCAGTGGTTCAAGGGCAAGAGC
CTCGACGGGCACGGGCCGATGGGGCCGTGGATCGTGACCAAGGGCGCGTTCGATCAGGACAAGCTGCACCTCGTCACCCG
CGTCAATGGCGTGGTGAAGCAGGATGCGAGCACGGGGCAGATGTATTTCAAGGTGCCGCGCATCATCGCGGAGCTGTCGC
TGGGGCAGACGCTGGAACCCGGCGACATCATCGCCAGCGGCACCCCACCGGGCGTGGGCTTCGCGCGCAAGCCGCAGGAA
TTCCTGAAGCCGGGCGACGTGATGGAGACGGAGATCGTCGGCATCGGCACGCTGCGCAACGAGATCAAGGCGGTGGGCTG
A

Upstream 100 bases:

>100_bases
GATGTTTCAGTGAGCTTCTGTTTTTCTTGGAATTGACAGATTTTGCGTCCTGATCATAGGTTGATCTGACAGTAAAAATG
AATAAAATAGCAGGGAGGCA

Downstream 100 bases:

>100_bases
ACGCCGGCAGCACGGGACGCGGACGCAACTGAAAGGGCGGCGCGGGAGCATCCGCGCCGCCGCTGCCTCGCGCCCTGTCA
GATGTGGGCGCGCAGGCTCA

Product: 2-keto-4-pentenoate hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MMDTGRRSFMVSVAAAAAAAAPLMVGPTHEAVAQTNDPGGALKLLSFRLRDGAPPRVGVALADGTLVDLTAEAARSGKTL
SFKPDDMVSLIAAGDTGLAEVRELVAQAAPGRVKLTEIIYLPPIPNPQRNIYAVGWNYLEHFAEGQAVRMTDTKYPEHPV
FFTKGTHSINGPFDPIPFDPKVSTQIDWEGELAIVIGKRGRNIPEAEAMDYVFGYSVINDTTARDVQNARHGGQWFKGKS
LDGHGPMGPWIVTKGAFDQDKLHLVTRVNGVVKQDASTGQMYFKVPRIIAELSLGQTLEPGDIIASGTPPGVGFARKPQE
FLKPGDVMETEIVGIGTLRNEIKAVG

Sequences:

>Translated_346_residues
MMDTGRRSFMVSVAAAAAAAAPLMVGPTHEAVAQTNDPGGALKLLSFRLRDGAPPRVGVALADGTLVDLTAEAARSGKTL
SFKPDDMVSLIAAGDTGLAEVRELVAQAAPGRVKLTEIIYLPPIPNPQRNIYAVGWNYLEHFAEGQAVRMTDTKYPEHPV
FFTKGTHSINGPFDPIPFDPKVSTQIDWEGELAIVIGKRGRNIPEAEAMDYVFGYSVINDTTARDVQNARHGGQWFKGKS
LDGHGPMGPWIVTKGAFDQDKLHLVTRVNGVVKQDASTGQMYFKVPRIIAELSLGQTLEPGDIIASGTPPGVGFARKPQE
FLKPGDVMETEIVGIGTLRNEIKAVG
>Mature_346_residues
MMDTGRRSFMVSVAAAAAAAAPLMVGPTHEAVAQTNDPGGALKLLSFRLRDGAPPRVGVALADGTLVDLTAEAARSGKTL
SFKPDDMVSLIAAGDTGLAEVRELVAQAAPGRVKLTEIIYLPPIPNPQRNIYAVGWNYLEHFAEGQAVRMTDTKYPEHPV
FFTKGTHSINGPFDPIPFDPKVSTQIDWEGELAIVIGKRGRNIPEAEAMDYVFGYSVINDTTARDVQNARHGGQWFKGKS
LDGHGPMGPWIVTKGAFDQDKLHLVTRVNGVVKQDASTGQMYFKVPRIIAELSLGQTLEPGDIIASGTPPGVGFARKPQE
FLKPGDVMETEIVGIGTLRNEIKAVG

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=307, Percent_Identity=37.1335504885993, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI40786394, Length=307, Percent_Identity=36.8078175895765, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI66348062, Length=195, Percent_Identity=37.9487179487179, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI215422413, Length=195, Percent_Identity=37.9487179487179, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI13654274, Length=195, Percent_Identity=37.9487179487179, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1787428, Length=191, Percent_Identity=34.0314136125654, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17557057, Length=207, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324161, Length=240, Percent_Identity=30.4166666666667, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572127, Length=262, Percent_Identity=44.6564885496183, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24663695, Length=326, Percent_Identity=31.9018404907975, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI28571789, Length=218, Percent_Identity=34.4036697247706, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 37124; Mature: 37124

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDTGRRSFMVSVAAAAAAAAPLMVGPTHEAVAQTNDPGGALKLLSFRLRDGAPPRVGVA
CCCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCCEEEEEEHCCCCCCCCEEEE
LADGTLVDLTAEAARSGKTLSFKPDDMVSLIAAGDTGLAEVRELVAQAAPGRVKLTEIIY
EECCEEEEEEHHHHHCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEE
LPPIPNPQRNIYAVGWNYLEHFAEGQAVRMTDTKYPEHPVFFTKGTHSINGPFDPIPFDP
ECCCCCCCCCEEEECHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCC
KVSTQIDWEGELAIVIGKRGRNIPEAEAMDYVFGYSVINDTTARDVQNARHGGQWFKGKS
CCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
LDGHGPMGPWIVTKGAFDQDKLHLVTRVNGVVKQDASTGQMYFKVPRIIAELSLGQTLEP
CCCCCCCCCEEEECCCCCCCCEEEEEHHCHHHCCCCCCCEEEEHHHHHHHHHHCCCCCCC
GDIIASGTPPGVGFARKPQEFLKPGDVMETEIVGIGTLRNEIKAVG
CCEEECCCCCCCCCCCCCHHHCCCCCCCEEEEEEEHHHHHHHHCCC
>Mature Secondary Structure
MMDTGRRSFMVSVAAAAAAAAPLMVGPTHEAVAQTNDPGGALKLLSFRLRDGAPPRVGVA
CCCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCCEEEEEEHCCCCCCCCEEEE
LADGTLVDLTAEAARSGKTLSFKPDDMVSLIAAGDTGLAEVRELVAQAAPGRVKLTEIIY
EECCEEEEEEHHHHHCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEE
LPPIPNPQRNIYAVGWNYLEHFAEGQAVRMTDTKYPEHPVFFTKGTHSINGPFDPIPFDP
ECCCCCCCCCEEEECHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCC
KVSTQIDWEGELAIVIGKRGRNIPEAEAMDYVFGYSVINDTTARDVQNARHGGQWFKGKS
CCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
LDGHGPMGPWIVTKGAFDQDKLHLVTRVNGVVKQDASTGQMYFKVPRIIAELSLGQTLEP
CCCCCCCCCEEEECCCCCCCCEEEEEHHCHHHCCCCCCCEEEEHHHHHHHHHHCCCCCCC
GDIIASGTPPGVGFARKPQEFLKPGDVMETEIVGIGTLRNEIKAVG
CCEEECCCCCCCCCCCCCHHHCCCCCCCEEEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]