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The map label for this gene is gsiB [H]

Identifier: 158423135

GI number: 158423135

Start: 1718713

End: 1720335

Strand: Direct

Name: gsiB [H]

Synonym: AZC_1511

Alternate gene names: 158423135

Gene position: 1718713-1720335 (Clockwise)

Preceding gene: 158423134

Following gene: 158423136

Centisome position: 32.01

GC content: 63.4

Gene sequence:

>1623_bases
ATGAACCGCCGTGAGTTTCTCACCACCGCTTCCGCCACCGCCGCTGCTGCCGGCGCCGCCGTTTCCGCGCCCGCCGTCTT
CTCTCCCGCCAAGGCGCAGGCGCGGTCCGAGACGCTGCTGATCGTCTCCGAGAGCGGGCCGAACAATCTGGACATCCACG
GCGTCGGCACCAACGTGCCCGGCTATGAGGCGAGCTGGAACTGCTACGACCGCCTCATTACCCACCCCATGAAGGACGTG
AACGGCACGCCCTATTACGACCGCGACAAGTTCGAGGGCGAACTGGCCGAGGACATGAGCATCGGCGACATGTCGGTGAC
GTTCAAGCTGAAGAAGGACGCCACCTTCCAGGACGGCACGCCCGTCACCGCCAAGGATGTGAAGTGGTCGCTGGACCGCG
CCGTCTCCGTCGGCGGCTTCCCCACCTTCCAGATGAAGGCGGGCTCGCTGGAGAAGCCCGAGCAGTTCGTGGTGGTGGAC
GACCGCACCATCCGCGTCGACTTCATCCGCAAGGACCGTCTGACCATTCCCGATCTCGCTGTGATCGTGCCCTGCGTCAT
CAATTCCGAGCTGGTGAAGAAGAACGCCACCGCGCAGGACCCCTGGGGCCTCGAATACACCAAGCAGAACACGGTGGGCT
CCGGCGCCTATAAGGTGACCAAGTGGACGCCCGGCACGGAAGTGATCTTCGAGCGCTTCGACAATTGGAAGGGCGGCCCG
CTGCCCAAGGTGAAGCGCGTGATCTGGCGCATGGTGCCATCTTCCGGCAACCGCCGCGCGCTCTTGGAGCGGGGCGATGC
GGACATCTCCTACGATCTGCCGAACAAGGACTTCGCCGAGCTGAAGTCCAACCCCAAGCTCAACATCATCTCCACGCCCT
ATTCCAACGGCGTGCAGTACATCGGCATGAACGTCACCAAGCCGCCCTTCAACAATCCGAAGGTGCGGCAGGCGGTGGCC
TATGCGCTGCCCTATGAGAAGATCATGGAAGCGGTGCTGTTCGGCCTCGCCAACCCCATGTTTGGCGCGCCAGCGGACTA
CCAGACCAAGGTCGCCTGGCCGCAGCCCACCAAATACAAGACCGACATCGCCAAGGCGAAGGCACTGCTGGCCGAGGCGG
GCTATGCGGACGGCTTCGAGACCACGCTCTCCTTCGACCTCGGCTTCGCCGGCGTGAACGAGCCGCTGTGCGTGCTCGTG
CAGGAGAGCCTCGCCCAGATCGGCATCAAGACCACCATCAACAAGATCCCTGGCGCCAACTGGCGCACCGAGCTGAACAA
GAAGGAGCTGCCGCTCTTCACCAACGTCTTCTCGGGCTGGCTGGACTATCCCGAATACTTCTTCTTCTGGTGCTACCACG
GCTCCAACTCGGTCTTTAACACCATGAGCTACCAGTCTGCGGCCATGGATGCCTTCATCGACGGCGCCCGCGCCGCCGCC
GCCAACGGCGACAAGGCGGCCTATGATGCGGACGTGAAGGGCATGGTGGACCTCGCCTTCGCCGACGTGCCGCGCGTGCC
GCTGTTCCAGCCTTATGTGAACGTCGCCATGCAGAAGAACATCACCGGCTACGAATACTGGTTCCACCGCCGGCTCGATT
ATCGCGCGCTCCAGAAGGGCTGA

Upstream 100 bases:

>100_bases
GATAACTTCATGTATAATAATGCTTTTTAAATTTTAGTATGCAATTGGCATAGTGCTTGAATGGTTGGGCGAGGCCGCTT
TCAGACGGGGATCCTTGCCT

Downstream 100 bases:

>100_bases
GTCCGCCTGCCGGGGTAGGGCGCGCGCGTCGCGCGCGTCCCGAATGCCCCCTCCCAACCCTCCCCCGCAAGCGGGAGAGG
GCTTTCTCGCTCAGTCTTCC

Product: twin-arginine translocation pathway signal precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNRREFLTTASATAAAAGAAVSAPAVFSPAKAQARSETLLIVSESGPNNLDIHGVGTNVPGYEASWNCYDRLITHPMKDV
NGTPYYDRDKFEGELAEDMSIGDMSVTFKLKKDATFQDGTPVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVD
DRTIRVDFIRKDRLTIPDLAVIVPCVINSELVKKNATAQDPWGLEYTKQNTVGSGAYKVTKWTPGTEVIFERFDNWKGGP
LPKVKRVIWRMVPSSGNRRALLERGDADISYDLPNKDFAELKSNPKLNIISTPYSNGVQYIGMNVTKPPFNNPKVRQAVA
YALPYEKIMEAVLFGLANPMFGAPADYQTKVAWPQPTKYKTDIAKAKALLAEAGYADGFETTLSFDLGFAGVNEPLCVLV
QESLAQIGIKTTINKIPGANWRTELNKKELPLFTNVFSGWLDYPEYFFFWCYHGSNSVFNTMSYQSAAMDAFIDGARAAA
ANGDKAAYDADVKGMVDLAFADVPRVPLFQPYVNVAMQKNITGYEYWFHRRLDYRALQKG

Sequences:

>Translated_540_residues
MNRREFLTTASATAAAAGAAVSAPAVFSPAKAQARSETLLIVSESGPNNLDIHGVGTNVPGYEASWNCYDRLITHPMKDV
NGTPYYDRDKFEGELAEDMSIGDMSVTFKLKKDATFQDGTPVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVD
DRTIRVDFIRKDRLTIPDLAVIVPCVINSELVKKNATAQDPWGLEYTKQNTVGSGAYKVTKWTPGTEVIFERFDNWKGGP
LPKVKRVIWRMVPSSGNRRALLERGDADISYDLPNKDFAELKSNPKLNIISTPYSNGVQYIGMNVTKPPFNNPKVRQAVA
YALPYEKIMEAVLFGLANPMFGAPADYQTKVAWPQPTKYKTDIAKAKALLAEAGYADGFETTLSFDLGFAGVNEPLCVLV
QESLAQIGIKTTINKIPGANWRTELNKKELPLFTNVFSGWLDYPEYFFFWCYHGSNSVFNTMSYQSAAMDAFIDGARAAA
ANGDKAAYDADVKGMVDLAFADVPRVPLFQPYVNVAMQKNITGYEYWFHRRLDYRALQKG
>Mature_540_residues
MNRREFLTTASATAAAAGAAVSAPAVFSPAKAQARSETLLIVSESGPNNLDIHGVGTNVPGYEASWNCYDRLITHPMKDV
NGTPYYDRDKFEGELAEDMSIGDMSVTFKLKKDATFQDGTPVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVD
DRTIRVDFIRKDRLTIPDLAVIVPCVINSELVKKNATAQDPWGLEYTKQNTVGSGAYKVTKWTPGTEVIFERFDNWKGGP
LPKVKRVIWRMVPSSGNRRALLERGDADISYDLPNKDFAELKSNPKLNIISTPYSNGVQYIGMNVTKPPFNNPKVRQAVA
YALPYEKIMEAVLFGLANPMFGAPADYQTKVAWPQPTKYKTDIAKAKALLAEAGYADGFETTLSFDLGFAGVNEPLCVLV
QESLAQIGIKTTINKIPGANWRTELNKKELPLFTNVFSGWLDYPEYFFFWCYHGSNSVFNTMSYQSAAMDAFIDGARAAA
ANGDKAAYDADVKGMVDLAFADVPRVPLFQPYVNVAMQKNITGYEYWFHRRLDYRALQKG

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=453, Percent_Identity=28.476821192053, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787762, Length=509, Percent_Identity=26.7190569744597, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789966, Length=442, Percent_Identity=25.5656108597285, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787495, Length=542, Percent_Identity=24.3542435424354, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787551, Length=356, Percent_Identity=25.2808988764045, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789887, Length=221, Percent_Identity=26.2443438914027, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59862; Mature: 59862

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRREFLTTASATAAAAGAAVSAPAVFSPAKAQARSETLLIVSESGPNNLDIHGVGTNVP
CCCHHEEEECCHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCCEEEEEECCCCC
GYEASWNCYDRLITHPMKDVNGTPYYDRDKFEGELAEDMSIGDMSVTFKLKKDATFQDGT
CCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEEEEECCCCCCCCC
PVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVDDRTIRVDFIRKDRLTIPDLA
CCCHHHCHHHHHHEEECCCCCEEEECCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHH
VIVPCVINSELVKKNATAQDPWGLEYTKQNTVGSGAYKVTKWTPGTEVIFERFDNWKGGP
HHHHHHHCHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCC
LPKVKRVIWRMVPSSGNRRALLERGDADISYDLPNKDFAELKSNPKLNIISTPYSNGVQY
CHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCHHHHHCCCCCEEEEECCCCCCEEE
IGMNVTKPPFNNPKVRQAVAYALPYEKIMEAVLFGLANPMFGAPADYQTKVAWPQPTKYK
EEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHH
TDIAKAKALLAEAGYADGFETTLSFDLGFAGVNEPLCVLVQESLAQIGIKTTINKIPGAN
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCC
WRTELNKKELPLFTNVFSGWLDYPEYFFFWCYHGSNSVFNTMSYQSAAMDAFIDGARAAA
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHC
ANGDKAAYDADVKGMVDLAFADVPRVPLFQPYVNVAMQKNITGYEYWFHRRLDYRALQKG
CCCCCCEECCCCCCCEEEHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MNRREFLTTASATAAAAGAAVSAPAVFSPAKAQARSETLLIVSESGPNNLDIHGVGTNVP
CCCHHEEEECCHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCCEEEEEECCCCC
GYEASWNCYDRLITHPMKDVNGTPYYDRDKFEGELAEDMSIGDMSVTFKLKKDATFQDGT
CCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEEEEECCCCCCCCC
PVTAKDVKWSLDRAVSVGGFPTFQMKAGSLEKPEQFVVVDDRTIRVDFIRKDRLTIPDLA
CCCHHHCHHHHHHEEECCCCCEEEECCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHH
VIVPCVINSELVKKNATAQDPWGLEYTKQNTVGSGAYKVTKWTPGTEVIFERFDNWKGGP
HHHHHHHCHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCC
LPKVKRVIWRMVPSSGNRRALLERGDADISYDLPNKDFAELKSNPKLNIISTPYSNGVQY
CHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCHHHHHCCCCCEEEEECCCCCCEEE
IGMNVTKPPFNNPKVRQAVAYALPYEKIMEAVLFGLANPMFGAPADYQTKVAWPQPTKYK
EEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHH
TDIAKAKALLAEAGYADGFETTLSFDLGFAGVNEPLCVLVQESLAQIGIKTTINKIPGAN
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCC
WRTELNKKELPLFTNVFSGWLDYPEYFFFWCYHGSNSVFNTMSYQSAAMDAFIDGARAAA
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHC
ANGDKAAYDADVKGMVDLAFADVPRVPLFQPYVNVAMQKNITGYEYWFHRRLDYRALQKG
CCCCCCEECCCCCCCEEEHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA