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The map label for this gene is narH [H]

Identifier: 158423050

GI number: 158423050

Start: 1627147

End: 1628673

Strand: Direct

Name: narH [H]

Synonym: AZC_1426

Alternate gene names: 158423050

Gene position: 1627147-1628673 (Clockwise)

Preceding gene: 158423049

Following gene: 158423051

Centisome position: 30.3

GC content: 64.51

Gene sequence:

>1527_bases
ATGAAGATCCGTGCTCAAATCGCGATGGTGCTGAACCTCGACAAGTGCATCGGCTGCCACACCTGTTCCGTCACCTGCAA
GAACGTGTGGACCAACCGCGAGGGCGTCGAATACGCCTGGTTCAACAATGTGGAGACCAAGCCCGGCGTCGGCTATCCCC
GCGAGTGGGAGAACCAGGACAAGTGGAACGGCGGCTGGCGGCGCAAGAAGAACGGCCGTATCGAGCCGCGCATCGGCGCC
AAGTGGCGGGTGCTCGCGAATATCTTCGCCAATCCGGACCTGCCGGAGATTGACGACTATTACGAGCCCTTCAATTTTGA
CTACGAGCATCTGCACACGGCGAAGGAGAGCAGGGCTTTCCCCACGGCCCGCCCGCGCTCGGCGGTCAGCGGCGAGCGGA
TGGAGAAGATCGAGTGGGGGCCGAACTGGGAGGAGATCCTCGGCGGTGAGTTCGCCAAGCGCTCGAAGGATGTGAATTTC
GAGGCCGTGCAGAAGGACATCTACGGCCAGTTCGAGAACACCTTCATGATGTATCTGCCGCGCCTGTGCGAGCACTGCCT
CAATCCGGCGTGCGCGGCCTCGTGCCCCTCCGGCGCCATCTACAAGCGCGAGGAAGACGGCATCGTCCTCATTGATCAGG
ACAAGTGCCGGGGCTGGCGCATGTGCGTTTCCGGCTGCCCCTACAAGAAGATCTATTACAACTGGTCTTCGGGAAAGTCG
GAGAAGTGCATCTTCTGCTATCCGCGCATCGAGGCGGGCCAGCCCACCGTCTGCTCGGAAACCTGCGTCGGCCGCATCCG
CTACCTCGGCGTGCTGCTCTATGATGCGGACCGCATCGAGGCCGCCGCCAGCGTGCCGGACGAGAAGGATCTCTATCAGG
CCCAGCTCGACCTCTTCCTCGACCCCAAGGATCCGGCGGTGATCGCGCAGGCGCGCGCCGATGGCGTGCCGGAAGCCTGG
CTTGAGGCGGCGCGCCATTCGCCGGTGTGGAAGATGGCCATGGAGTGGAAGGTGGCCTTCCCGCTCCATCCCGAATACCG
CACCCTGCCCATGGTCTGGTACGTGCCCCCGCTCTCGCCCATCCAGTCGGCCGCCGCGGCCGGCCAGATGGGGGTGAATG
GCGAGATGCCGGACGTGCGCTCGCTGCGCATCCCGCTGCGCTATCTCGCCAACCTGCTCACGGCGGGAGATGAGGAGCCG
GTGGCCCTCGCCCTTGAGCGGATGCTTGCCATGCGCGGCTACATGCGGGCCAAGACCGTGGACGGGCGTCTTGATGAGCG
CATCGCCGCGAAGGTCGGCCTCACGGGCGCGCATATCGAGGACATGTATCAGGTGATGGCGATCGCCAATTACGAGGATC
GCTTCGTCATCCCCACCGCCCATCGCGAGTGGAGCGAGGATGCCTACGACCTGCGCGGCTCCTGCGGCTTCTCCTTCGGC
AACGGCTGCTCGGGCGGCAGCACGGAGACCAATTTGTTCGGCACCCGCCAGACGCCCAAGGCGCGCAATCCCATGGAGGT
GGCGTGA

Upstream 100 bases:

>100_bases
ACGAGTTCATCGTGGTGCGCAAGATGGCGCGGGTGGACTGGCTCGAAGGTCCGCTCGCCCCCGATCATTCCCCGAACACC
GCGCCGATGGAGGCTGCACA

Downstream 100 bases:

>100_bases
TGGCCATCGAAACCCTCGCCTTCAAGGCGCTCTCCGCGCTGCTGAGCTATCCGGACGCCGGCCTTGCCGCCGGCATTCCG
GAGATCAAGGCCGCGCTCGC

Product: nitrate reductase subunit beta protein

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGVGYPREWENQDKWNGGWRRKKNGRIEPRIGA
KWRVLANIFANPDLPEIDDYYEPFNFDYEHLHTAKESRAFPTARPRSAVSGERMEKIEWGPNWEEILGGEFAKRSKDVNF
EAVQKDIYGQFENTFMMYLPRLCEHCLNPACAASCPSGAIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIEAAASVPDEKDLYQAQLDLFLDPKDPAVIAQARADGVPEAW
LEAARHSPVWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGQMGVNGEMPDVRSLRIPLRYLANLLTAGDEEP
VALALERMLAMRGYMRAKTVDGRLDERIAAKVGLTGAHIEDMYQVMAIANYEDRFVIPTAHREWSEDAYDLRGSCGFSFG
NGCSGGSTETNLFGTRQTPKARNPMEVA

Sequences:

>Translated_508_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGVGYPREWENQDKWNGGWRRKKNGRIEPRIGA
KWRVLANIFANPDLPEIDDYYEPFNFDYEHLHTAKESRAFPTARPRSAVSGERMEKIEWGPNWEEILGGEFAKRSKDVNF
EAVQKDIYGQFENTFMMYLPRLCEHCLNPACAASCPSGAIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIEAAASVPDEKDLYQAQLDLFLDPKDPAVIAQARADGVPEAW
LEAARHSPVWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGQMGVNGEMPDVRSLRIPLRYLANLLTAGDEEP
VALALERMLAMRGYMRAKTVDGRLDERIAAKVGLTGAHIEDMYQVMAIANYEDRFVIPTAHREWSEDAYDLRGSCGFSFG
NGCSGGSTETNLFGTRQTPKARNPMEVA
>Mature_508_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGVGYPREWENQDKWNGGWRRKKNGRIEPRIGA
KWRVLANIFANPDLPEIDDYYEPFNFDYEHLHTAKESRAFPTARPRSAVSGERMEKIEWGPNWEEILGGEFAKRSKDVNF
EAVQKDIYGQFENTFMMYLPRLCEHCLNPACAASCPSGAIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIEAAASVPDEKDLYQAQLDLFLDPKDPAVIAQARADGVPEAW
LEAARHSPVWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGQMGVNGEMPDVRSLRIPLRYLANLLTAGDEEP
VALALERMLAMRGYMRAKTVDGRLDERIAAKVGLTGAHIEDMYQVMAIANYEDRFVIPTAHREWSEDAYDLRGSCGFSFG
NGCSGGSTETNLFGTRQTPKARNPMEVA

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=498, Percent_Identity=72.8915662650602, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=496, Percent_Identity=70.3629032258064, Blast_Score=770, Evalue=0.0,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=43.5643564356436, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=44.4444444444444, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI2367345, Length=142, Percent_Identity=26.7605633802817, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789370, Length=107, Percent_Identity=37.3831775700935, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=34.4444444444444, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57571; Mature: 57571

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGVGYPREWENQD
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWRRKKNGRIEPRIGAKWRVLANIFANPDLPEIDDYYEPFNFDYEHLHTAKESRAF
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCC
PTARPRSAVSGERMEKIEWGPNWEEILGGEFAKRSKDVNFEAVQKDIYGQFENTFMMYLP
CCCCCCCCCCCHHHHHCCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACAASCPSGAIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
HHHHHHCCCHHHCCCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIEAAASVPDEKDLYQAQLDLFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEECCHHHHHHHCCCCHHHHHHHHHHEEE
DPKDPAVIAQARADGVPEAWLEAARHSPVWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCEEEEEHHCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCCH
IQSAAAAGQMGVNGEMPDVRSLRIPLRYLANLLTAGDEEPVALALERMLAMRGYMRAKTV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DGRLDERIAAKVGLTGAHIEDMYQVMAIANYEDRFVIPTAHREWSEDAYDLRGSCGFSFG
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCCCCCC
NGCSGGSTETNLFGTRQTPKARNPMEVA
CCCCCCCCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGVEYAWFNNVETKPGVGYPREWENQD
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWRRKKNGRIEPRIGAKWRVLANIFANPDLPEIDDYYEPFNFDYEHLHTAKESRAF
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCC
PTARPRSAVSGERMEKIEWGPNWEEILGGEFAKRSKDVNFEAVQKDIYGQFENTFMMYLP
CCCCCCCCCCCHHHHHCCCCCCHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACAASCPSGAIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWSSGKS
HHHHHHCCCHHHCCCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIEAAASVPDEKDLYQAQLDLFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEECCHHHHHHHCCCCHHHHHHHHHHEEE
DPKDPAVIAQARADGVPEAWLEAARHSPVWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCEEEEEHHCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCCH
IQSAAAAGQMGVNGEMPDVRSLRIPLRYLANLLTAGDEEPVALALERMLAMRGYMRAKTV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DGRLDERIAAKVGLTGAHIEDMYQVMAIANYEDRFVIPTAHREWSEDAYDLRGSCGFSFG
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCCCCCC
NGCSGGSTETNLFGTRQTPKARNPMEVA
CCCCCCCCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]