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The map label for this gene is asnB [H]

Identifier: 158422989

GI number: 158422989

Start: 1542251

End: 1544020

Strand: Direct

Name: asnB [H]

Synonym: AZC_1365

Alternate gene names: 158422989

Gene position: 1542251-1544020 (Clockwise)

Preceding gene: 158422986

Following gene: 158422990

Centisome position: 28.72

GC content: 63.73

Gene sequence:

>1770_bases
ATGTGCGGGATCTGCGGTGAGGTGAACAGGAACGGCTCGGTCCGGATGGCGCCGCTTGAGGCTATGTCGGATGCGCTGTC
ACCGCGGGGACCGGACGGTTTCGGCCTGCTTGCCCAGGGCGACGTGGCGCTGGCCCATCGCAGGCTCAGCATTCTCGATC
TCAAGGCCACCTCCCAGCAGCCGATGGTGGACAGCCAACTGGGGCTCGCCATCGCTTTCAACGGCTGCATCTACAATTTT
CGCGACCTCCGTCAGGACCTGAAAGCCAAGGGCTACACCTTCTTCTCCGAAGGCGACACCGAGGTGATCCTCAAGGCCTA
TCATGCTTGGGGCGCCGATTGCGTTCTGCGGCTGAAGGGCATGTTCGCCTTTGCCATTTGGGAGATGTGGACAGGGCGTG
TCGTGCTGGCGCGGGACAGGCTGGGCATCAAGCCGCTCTATCTCGCGGACGTGGGCTCTTCCTTGCGCTTTGCCTCCACC
TTGCCGGCCCTGCTGGCGGGCGGCGGGGTGGATACGGCCATCGATCCTGTCGGCCTTCACCATTACATGAGCTTTCACAG
CGTAGTGCCGGCACCCCGGACCATCCTGCGAGGCGTGCGCAAGCTGCCCCCGGCGACGCTGCTGACGCTGGAACCCGACT
GCCAACGCAGCGAGCGGCAATACTGGTCCGTCCGCATGGGAGGCGATACCGACACGGACGATATGGGCGAGGAGGAGTGG
CGCGAGACGCTCATCCACTGCCTTCTGCGGGCCGTGGAGCGGCGGACGGTCTCAGACGTACCGGTGGGCGTCTTGCTCTC
CGGCGGGCTCGACAGCTCTCTTCTCGTGGCCCTGCTGGCCCAACTGGGGCATGAGGACATCCGCACCTTTTCCGTCGGCT
TCGAAGCCGTGGGCGGGCTGAAGGGCGACGAGTTCGAATATTCCGATCTCATGGCCCGCGCTTACGGCACAGACCACACC
AAGATCGTGATCTCGCCGGAGCGCGTGCTCTCGGCGCTGCCGGGCGTCATCGGCGCCATGTCCGAGCCCATGATGAGCCA
CGATTGCGTGGGCTTCTATCTGCTGGCGGAGGAAGTGTCCCGGCATGTGAAGGTGGTGCAGAGCGGGCAGGGGGCGGACG
AGACCTTCGCCGGTTATCACTGGCATGAGAAGATGCTCACCGCCGCCGATCCGCTGGATGATTACGCACGGCTCTATTTC
GACCGGGATCATGCGGAGATGGCTCACGCCCTCTCTCCGGACATGATCTCGGGGGACCACAGCCGGATATTTGTAAAGGA
CTTCTTCGATCGCTGCGGCGAGACCTCGCCCATCAACAAGACCCTGCAGATCGACACGGAGATCATGCTGGTCGACGATC
CCGTAAAACGCGTGGACAACATGACCATGGCCTTCGGCCTCGAGACCCGCGTCCCCTTCCTCGACCATGAAATGGTGGAA
CTGGCCACGCGCATCCCCTCGGAACTGAAGGTGAAATCGGGCGGCAAGCACATATTGAAGGAGGCTGCGCGCCGTATCCT
GCCCCCTGAAGTGGTGGACCGGCCGAAGGGCTATTTCCCGGTGCCGGCGCTCAAATACATCCGCGGCCCCTATCTCGATT
TCGCGCGGGACGTGCTGAACCAGCAGGCCGCGCGCGATCGCGGCATCTTCCGGCAGGACTATGTGGAGGGCCTGCTCGGA
GACCCCGAAGGGAGCCTCACGCCAAAGGGGCATTCCAAGCTCTGGCAGGTCACCGTGCTGGAACACTGGCTCCAGACGCA
CGGGGTCTGA

Upstream 100 bases:

>100_bases
TGCCGCCTCCTACTCCCCTGGCACTTCGGGCTATGGCCTGCCCCTTCTAGGGGTACCGGGGGAGCCCTTCGCCGGGACGT
CAAGCACAGGCAGGATGATC

Downstream 100 bases:

>100_bases
AGGGGGCTTCGCGTCGTGCAGCGCCCGCGCCACGCCTGAGAGATTTGGAAAGGAGGAGGCCCATGTGCCGGTGGATGGCC
TATTCCGGTGAGCCGATTGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MCGICGEVNRNGSVRMAPLEAMSDALSPRGPDGFGLLAQGDVALAHRRLSILDLKATSQQPMVDSQLGLAIAFNGCIYNF
RDLRQDLKAKGYTFFSEGDTEVILKAYHAWGADCVLRLKGMFAFAIWEMWTGRVVLARDRLGIKPLYLADVGSSLRFAST
LPALLAGGGVDTAIDPVGLHHYMSFHSVVPAPRTILRGVRKLPPATLLTLEPDCQRSERQYWSVRMGGDTDTDDMGEEEW
RETLIHCLLRAVERRTVSDVPVGVLLSGGLDSSLLVALLAQLGHEDIRTFSVGFEAVGGLKGDEFEYSDLMARAYGTDHT
KIVISPERVLSALPGVIGAMSEPMMSHDCVGFYLLAEEVSRHVKVVQSGQGADETFAGYHWHEKMLTAADPLDDYARLYF
DRDHAEMAHALSPDMISGDHSRIFVKDFFDRCGETSPINKTLQIDTEIMLVDDPVKRVDNMTMAFGLETRVPFLDHEMVE
LATRIPSELKVKSGGKHILKEAARRILPPEVVDRPKGYFPVPALKYIRGPYLDFARDVLNQQAARDRGIFRQDYVEGLLG
DPEGSLTPKGHSKLWQVTVLEHWLQTHGV

Sequences:

>Translated_589_residues
MCGICGEVNRNGSVRMAPLEAMSDALSPRGPDGFGLLAQGDVALAHRRLSILDLKATSQQPMVDSQLGLAIAFNGCIYNF
RDLRQDLKAKGYTFFSEGDTEVILKAYHAWGADCVLRLKGMFAFAIWEMWTGRVVLARDRLGIKPLYLADVGSSLRFAST
LPALLAGGGVDTAIDPVGLHHYMSFHSVVPAPRTILRGVRKLPPATLLTLEPDCQRSERQYWSVRMGGDTDTDDMGEEEW
RETLIHCLLRAVERRTVSDVPVGVLLSGGLDSSLLVALLAQLGHEDIRTFSVGFEAVGGLKGDEFEYSDLMARAYGTDHT
KIVISPERVLSALPGVIGAMSEPMMSHDCVGFYLLAEEVSRHVKVVQSGQGADETFAGYHWHEKMLTAADPLDDYARLYF
DRDHAEMAHALSPDMISGDHSRIFVKDFFDRCGETSPINKTLQIDTEIMLVDDPVKRVDNMTMAFGLETRVPFLDHEMVE
LATRIPSELKVKSGGKHILKEAARRILPPEVVDRPKGYFPVPALKYIRGPYLDFARDVLNQQAARDRGIFRQDYVEGLLG
DPEGSLTPKGHSKLWQVTVLEHWLQTHGV
>Mature_589_residues
MCGICGEVNRNGSVRMAPLEAMSDALSPRGPDGFGLLAQGDVALAHRRLSILDLKATSQQPMVDSQLGLAIAFNGCIYNF
RDLRQDLKAKGYTFFSEGDTEVILKAYHAWGADCVLRLKGMFAFAIWEMWTGRVVLARDRLGIKPLYLADVGSSLRFAST
LPALLAGGGVDTAIDPVGLHHYMSFHSVVPAPRTILRGVRKLPPATLLTLEPDCQRSERQYWSVRMGGDTDTDDMGEEEW
RETLIHCLLRAVERRTVSDVPVGVLLSGGLDSSLLVALLAQLGHEDIRTFSVGFEAVGGLKGDEFEYSDLMARAYGTDHT
KIVISPERVLSALPGVIGAMSEPMMSHDCVGFYLLAEEVSRHVKVVQSGQGADETFAGYHWHEKMLTAADPLDDYARLYF
DRDHAEMAHALSPDMISGDHSRIFVKDFFDRCGETSPINKTLQIDTEIMLVDDPVKRVDNMTMAFGLETRVPFLDHEMVE
LATRIPSELKVKSGGKHILKEAARRILPPEVVDRPKGYFPVPALKYIRGPYLDFARDVLNQQAARDRGIFRQDYVEGLLG
DPEGSLTPKGHSKLWQVTVLEHWLQTHGV

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=544, Percent_Identity=29.0441176470588, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17560178, Length=507, Percent_Identity=27.0216962524655, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=549, Percent_Identity=27.1402550091075, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71993933, Length=549, Percent_Identity=27.1402550091075, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325403, Length=479, Percent_Identity=25.2609603340292, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321563, Length=483, Percent_Identity=25.6728778467909, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45553209, Length=560, Percent_Identity=26.25, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 65293; Mature: 65293

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGICGEVNRNGSVRMAPLEAMSDALSPRGPDGFGLLAQGDVALAHRRLSILDLKATSQQ
CCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHEEEEECCCCC
PMVDSQLGLAIAFNGCIYNFRDLRQDLKAKGYTFFSEGDTEVILKAYHAWGADCVLRLKG
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCHHHHHHHHH
MFAFAIWEMWTGRVVLARDRLGIKPLYLADVGSSLRFASTLPALLAGGGVDTAIDPVGLH
HHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
HYMSFHSVVPAPRTILRGVRKLPPATLLTLEPDCQRSERQYWSVRMGGDTDTDDMGEEEW
HHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHH
RETLIHCLLRAVERRTVSDVPVGVLLSGGLDSSLLVALLAQLGHEDIRTFSVGFEAVGGL
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
KGDEFEYSDLMARAYGTDHTKIVISPERVLSALPGVIGAMSEPMMSHDCVGFYLLAEEVS
CCCCCCHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHH
RHVKVVQSGQGADETFAGYHWHEKMLTAADPLDDYARLYFDRDHAEMAHALSPDMISGDH
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCC
SRIFVKDFFDRCGETSPINKTLQIDTEIMLVDDPVKRVDNMTMAFGLETRVPFLDHEMVE
CEEHHHHHHHHCCCCCCCCCEEEECEEEEEECCHHHHHCCCEEEEECCCCCCCHHHHHHH
LATRIPSELKVKSGGKHILKEAARRILPPEVVDRPKGYFPVPALKYIRGPYLDFARDVLN
HHHHCCCCEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHH
QQAARDRGIFRQDYVEGLLGDPEGSLTPKGHSKLWQVTVLEHWLQTHGV
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGICGEVNRNGSVRMAPLEAMSDALSPRGPDGFGLLAQGDVALAHRRLSILDLKATSQQ
CCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHEEEEECCCCC
PMVDSQLGLAIAFNGCIYNFRDLRQDLKAKGYTFFSEGDTEVILKAYHAWGADCVLRLKG
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCHHHHHHHHH
MFAFAIWEMWTGRVVLARDRLGIKPLYLADVGSSLRFASTLPALLAGGGVDTAIDPVGLH
HHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
HYMSFHSVVPAPRTILRGVRKLPPATLLTLEPDCQRSERQYWSVRMGGDTDTDDMGEEEW
HHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHH
RETLIHCLLRAVERRTVSDVPVGVLLSGGLDSSLLVALLAQLGHEDIRTFSVGFEAVGGL
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
KGDEFEYSDLMARAYGTDHTKIVISPERVLSALPGVIGAMSEPMMSHDCVGFYLLAEEVS
CCCCCCHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHH
RHVKVVQSGQGADETFAGYHWHEKMLTAADPLDDYARLYFDRDHAEMAHALSPDMISGDH
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCC
SRIFVKDFFDRCGETSPINKTLQIDTEIMLVDDPVKRVDNMTMAFGLETRVPFLDHEMVE
CEEHHHHHHHHCCCCCCCCCEEEECEEEEEECCHHHHHCCCEEEEECCCCCCCHHHHHHH
LATRIPSELKVKSGGKHILKEAARRILPPEVVDRPKGYFPVPALKYIRGPYLDFARDVLN
HHHHCCCCEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHH
QQAARDRGIFRQDYVEGLLGDPEGSLTPKGHSKLWQVTVLEHWLQTHGV
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]