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The map label for this gene is phaB [H]

Identifier: 158422947

GI number: 158422947

Start: 1481191

End: 1482000

Strand: Reverse

Name: phaB [H]

Synonym: AZC_1323

Alternate gene names: 158422947

Gene position: 1482000-1481191 (Counterclockwise)

Preceding gene: 158422949

Following gene: 158422946

Centisome position: 27.6

GC content: 69.88

Gene sequence:

>810_bases
ATGAACTGGATCGACCTTGAGCAGGTCACCCTGCGCTACACGCTGTCCGGCAGCGGCCCGGTGCAACTGGTGCTGATCCA
TGAGCTGGGCGGCAGCCTCGAGAGCTTCGATGCGCTCATGCCACGCCTGGAGCGTGACTTTCGGGTGCTGCGCTATGACC
AGCGCGGCGCCGGCTGGTCCGAGAAGCCCCGCAAGCCGTTCACGATCGCAGACCATGCGCGGGATCTGCGCCAGCTGCTC
GACGCGCTCGGTCTCGCCGGCCCGTTTTGCCTCGCCGGCGTCGCGGCCGGCGCGGCCATTGCCGTGGTGCACGCGCTCAA
CGATCCGGCCGCCGTGTATGGGCTCGCCCTCTGCTCGCCCGCGCTGACGGTGGCGGAAGACCGCGTCCGCTATCTGGTGG
ACCGGTCGGAGCGTGCCATGCGCGAGGGCATGGCCGCGATCGTGGACGCGAGCCTCGCCCGCTCGTTCCCGTCCGTGGTG
CGGCGGGACCCGGCGGCTTATGCCGCCTACCGCGCGCGGTTCCTTGCCAGCGATCCGGTGGGCTACGCCTACGCCAACAT
GGCGCTGGCCGATGTGCGGCTGGACGGGCAGCTCCGGGCCCTCGACCTGCCATGTCTTGTGCTCGCCGGAACGCACGATC
TGCTGCGTCCGCCAGAGGCCGTCCGTGCCCTCTCGGCGCAACTGCCGCGTGCCATTTATGCGGAGATCGACAGTGGCCAC
ATCATGCCGGTTCAGGCCCCCGAGGCGATGGCCACGCATCTGCGGCACTTCTTCGCCAGCGTGACCCGCGACGCGCTGAT
GCGAGCCTGA

Upstream 100 bases:

>100_bases
CACCTCCATCGGCGGTTGCCGCCGCACGGACGCTCGTGATGGACGCCGTTTTGGCAGGCACCTTTCCCGACGTCAAATCC
CCTTTCGGGCCTGTTTCGCC

Downstream 100 bases:

>100_bases
TCAGGAGGCGATGATGACCGACCCCAAGGGACTCCTCCTCGTCACCATGGAGCCTCCGGCGAGCCTGGAAGAAGAGTTCA
ACGACTGGTACGACACCGAG

Product: alpha/beta hydrolase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNWIDLEQVTLRYTLSGSGPVQLVLIHELGGSLESFDALMPRLERDFRVLRYDQRGAGWSEKPRKPFTIADHARDLRQLL
DALGLAGPFCLAGVAAGAAIAVVHALNDPAAVYGLALCSPALTVAEDRVRYLVDRSERAMREGMAAIVDASLARSFPSVV
RRDPAAYAAYRARFLASDPVGYAYANMALADVRLDGQLRALDLPCLVLAGTHDLLRPPEAVRALSAQLPRAIYAEIDSGH
IMPVQAPEAMATHLRHFFASVTRDALMRA

Sequences:

>Translated_269_residues
MNWIDLEQVTLRYTLSGSGPVQLVLIHELGGSLESFDALMPRLERDFRVLRYDQRGAGWSEKPRKPFTIADHARDLRQLL
DALGLAGPFCLAGVAAGAAIAVVHALNDPAAVYGLALCSPALTVAEDRVRYLVDRSERAMREGMAAIVDASLARSFPSVV
RRDPAAYAAYRARFLASDPVGYAYANMALADVRLDGQLRALDLPCLVLAGTHDLLRPPEAVRALSAQLPRAIYAEIDSGH
IMPVQAPEAMATHLRHFFASVTRDALMRA
>Mature_269_residues
MNWIDLEQVTLRYTLSGSGPVQLVLIHELGGSLESFDALMPRLERDFRVLRYDQRGAGWSEKPRKPFTIADHARDLRQLL
DALGLAGPFCLAGVAAGAAIAVVHALNDPAAVYGLALCSPALTVAEDRVRYLVDRSERAMREGMAAIVDASLARSFPSVV
RRDPAAYAAYRARFLASDPVGYAYANMALADVRLDGQLRALDLPCLVLAGTHDLLRPPEAVRALSAQLPRAIYAEIDSGH
IMPVQAPEAMATHLRHFFASVTRDALMRA

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 29300; Mature: 29300

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWIDLEQVTLRYTLSGSGPVQLVLIHELGGSLESFDALMPRLERDFRVLRYDQRGAGWS
CCCCCCEEEEEEEEECCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCC
EKPRKPFTIADHARDLRQLLDALGLAGPFCLAGVAAGAAIAVVHALNDPAAVYGLALCSP
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
ALTVAEDRVRYLVDRSERAMREGMAAIVDASLARSFPSVVRRDPAAYAAYRARFLASDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
GYAYANMALADVRLDGQLRALDLPCLVLAGTHDLLRPPEAVRALSAQLPRAIYAEIDSGH
CHHHHHHHHEEEEECCCEEEECCCEEEEECCHHHHCCHHHHHHHHHHCCHHHHHCCCCCC
IMPVQAPEAMATHLRHFFASVTRDALMRA
EEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNWIDLEQVTLRYTLSGSGPVQLVLIHELGGSLESFDALMPRLERDFRVLRYDQRGAGWS
CCCCCCEEEEEEEEECCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCC
EKPRKPFTIADHARDLRQLLDALGLAGPFCLAGVAAGAAIAVVHALNDPAAVYGLALCSP
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
ALTVAEDRVRYLVDRSERAMREGMAAIVDASLARSFPSVVRRDPAAYAAYRARFLASDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
GYAYANMALADVRLDGQLRALDLPCLVLAGTHDLLRPPEAVRALSAQLPRAIYAEIDSGH
CHHHHHHHHEEEEECCCEEEECCCEEEEECCHHHHCCHHHHHHHHHHCCHHHHHCCCCCC
IMPVQAPEAMATHLRHFFASVTRDALMRA
EEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]