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The map label for this gene is pcaD [H]

Identifier: 158422945

GI number: 158422945

Start: 1479758

End: 1480567

Strand: Reverse

Name: pcaD [H]

Synonym: AZC_1321

Alternate gene names: 158422945

Gene position: 1480567-1479758 (Counterclockwise)

Preceding gene: 158422946

Following gene: 158422944

Centisome position: 27.57

GC content: 69.75

Gene sequence:

>810_bases
ATGCCCCGAGCCAACCCCAACAGCATCGAGATCGACGGACTGACCTTCCATTACCGGGTGGACGGCCCCGAGGGCGCGCA
CTGGCTGGTGTTCAGCAATTCCCTTATGACCGACCTGTCCCTATGGGACGATCAGGCGGCCGCGTTCGGGGAAAGCTTCC
GCATCCTGCGCTATGACCAGCGGGGTCACGGGCGGACGGGCATCCCGGCCGAAGACTGCACCATGGAGCGTCTTGCCGCC
GATCTGATCGGCCTCATGGATGCACTCGGCATCGCGCGCACCCATCTCGTCGGCATCTCCATGGGCGCCGCGACATGTCT
CGGTGCGGCCCTCGCGCAGCCTCGGCGCGTGGCCAGCCTCGTGATGGCGGACGGTCAGGCGGCGACACCGACGGGTGGCG
CACAAGCGTGGGAGGAGCGGATCGCCCTCGCCCGCACCCAAGGCATGGCCGGGCTCGCGGACCCCACCATCGCGCGCTGG
TTCCAGCCCGCCAGCGTCGAGACGAACGGAGAGGGTGTCCGCCGCGTGGCGGGCATGATAGCGGCGACGCCCCTCGACGG
CTTCCTGCGCGCGGTGCGCGCGCTCCAACGCTTCGATTTCCGGCCGGGCCTTACGGCCCTCGCCTGCCCCGCACTCATGC
TTGCCGGCGCGGCGGACGGCGCGCTGCCGAAAACCCTGGCTGGCCTCGCCGAGGCCGTGCCGGACGGCCGATTCGTCGAG
ATCCCCGATGCCGGCCACCTGCCCAATATTGAGCAGCCAGGCGCCTTCAACGCGGCGCTGGCTACCTTTCTGAAGAGAGG
CGCGGCATGA

Upstream 100 bases:

>100_bases
CCGCAGGGCTTTGAGCCCCTGCGGAGCGCACTCGCGTCGGTGGCCGGCATCGGGGCGCAGATGTTCAATCTCTATGCCCC
CTATTTCCGGGGTTGAGCCG

Downstream 100 bases:

>100_bases
GGATCAAGGATCTCCCCCTGGACGAGATGACGCCCGAGCAGCGGGCGGTGGCGGAAGAGGCCATCTCGGGCAAGCGCGGC
CGCGTTCCCGCGCCCCTCCG

Product: alpha/beta hydrolase

Products: NA

Alternate protein names: 3-oxoadipate enol-lactonase I; Beta-ketoadipate enol-lactone hydrolase I; Enol-lactone hydrolase I [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPRANPNSIEIDGLTFHYRVDGPEGAHWLVFSNSLMTDLSLWDDQAAAFGESFRILRYDQRGHGRTGIPAEDCTMERLAA
DLIGLMDALGIARTHLVGISMGAATCLGAALAQPRRVASLVMADGQAATPTGGAQAWEERIALARTQGMAGLADPTIARW
FQPASVETNGEGVRRVAGMIAATPLDGFLRAVRALQRFDFRPGLTALACPALMLAGAADGALPKTLAGLAEAVPDGRFVE
IPDAGHLPNIEQPGAFNAALATFLKRGAA

Sequences:

>Translated_269_residues
MPRANPNSIEIDGLTFHYRVDGPEGAHWLVFSNSLMTDLSLWDDQAAAFGESFRILRYDQRGHGRTGIPAEDCTMERLAA
DLIGLMDALGIARTHLVGISMGAATCLGAALAQPRRVASLVMADGQAATPTGGAQAWEERIALARTQGMAGLADPTIARW
FQPASVETNGEGVRRVAGMIAATPLDGFLRAVRALQRFDFRPGLTALACPALMLAGAADGALPKTLAGLAEAVPDGRFVE
IPDAGHLPNIEQPGAFNAALATFLKRGAA
>Mature_268_residues
PRANPNSIEIDGLTFHYRVDGPEGAHWLVFSNSLMTDLSLWDDQAAAFGESFRILRYDQRGHGRTGIPAEDCTMERLAAD
LIGLMDALGIARTHLVGISMGAATCLGAALAQPRRVASLVMADGQAATPTGGAQAWEERIALARTQGMAGLADPTIARWF
QPASVETNGEGVRRVAGMIAATPLDGFLRAVRALQRFDFRPGLTALACPALMLAGAADGALPKTLAGLAEAVPDGRFVEI
PDAGHLPNIEQPGAFNAALATFLKRGAA

Specific function: Unknown

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787244, Length=239, Percent_Identity=27.1966527196653, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012790 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.24 [H]

Molecular weight: Translated: 28284; Mature: 28152

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRANPNSIEIDGLTFHYRVDGPEGAHWLVFSNSLMTDLSLWDDQAAAFGESFRILRYDQ
CCCCCCCEEEECCEEEEEEECCCCCCEEEEECCCHHHHHHHHCHHHHHHCCCEEEEEECC
RGHGRTGIPAEDCTMERLAADLIGLMDALGIARTHLVGISMGAATCLGAALAQPRRVASL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
VMADGQAATPTGGAQAWEERIALARTQGMAGLADPTIARWFQPASVETNGEGVRRVAGMI
HHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHH
AATPLDGFLRAVRALQRFDFRPGLTALACPALMLAGAADGALPKTLAGLAEAVPDGRFVE
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEE
IPDAGHLPNIEQPGAFNAALATFLKRGAA
CCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRANPNSIEIDGLTFHYRVDGPEGAHWLVFSNSLMTDLSLWDDQAAAFGESFRILRYDQ
CCCCCCEEEECCEEEEEEECCCCCCEEEEECCCHHHHHHHHCHHHHHHCCCEEEEEECC
RGHGRTGIPAEDCTMERLAADLIGLMDALGIARTHLVGISMGAATCLGAALAQPRRVASL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
VMADGQAATPTGGAQAWEERIALARTQGMAGLADPTIARWFQPASVETNGEGVRRVAGMI
HHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHH
AATPLDGFLRAVRALQRFDFRPGLTALACPALMLAGAADGALPKTLAGLAEAVPDGRFVE
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEE
IPDAGHLPNIEQPGAFNAALATFLKRGAA
CCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8181753 [H]