| Definition | Azorhizobium caulinodans ORS 571, complete genome. |
|---|---|
| Accession | NC_009937 |
| Length | 5,369,772 |
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The map label for this gene is slt [H]
Identifier: 158422866
GI number: 158422866
Start: 1391585
End: 1393771
Strand: Reverse
Name: slt [H]
Synonym: AZC_1242
Alternate gene names: 158422866
Gene position: 1393771-1391585 (Counterclockwise)
Preceding gene: 158422870
Following gene: 158422865
Centisome position: 25.96
GC content: 69.87
Gene sequence:
>2187_bases ATGATGCCGTCTGTCCGTCTCCTGCTCTCATGCACCGCCCTCGCCCTGGCGCTTGCCGTGGCGCCGCCGGCCATGGCGGC CCAGGACAGCGACGCCCCCACCAAGAAAACCGACACCAAGAAGGCCGACGCGAAGAAGGCGGACCCGAAGAAGGACGCCA AGAAAGACGCGAAGAAGCCGGACGCCAAATCCAAGGCCGCCGCCAAGCCTGCGACCCATAGCCCCGGCCGCGGCGTGGCC GCCCGCCCGGCGCCCAAGGCCGCGCCGCTGACGCTCGCCCCCACCGCCGCCATCGCCACCGGCGAACTCGGCGAATTGCG CAATGCGGTGATCGCTGCCAAGGCCGGCCGCGGCACGGAAGCGCTCCAGATGGCGCGCCAGCTGGAAGACCCGGTGGCGC GCAACCTCGTGGTCTGGCTGGTCATCCGCAACGCGCCGAACGACTACGGCTTCACCAACATCGCCGCCTTCCTGCGCGAG AAGCCGGGCTGGCCGACGCCCGGCACCCTGCGCCGCCGCGCCGAGCGCGTGCTGCTGGCGGAGAACAAGGATCCGGCCAC CGTCCGCGCCTTCTTCTCGGAACAGGACCCGATCTCCGGCGAGGGCAAGATTGCGCTCGCCCGCGCCATGGCCGCCTCCG GCGACCGCAACACGACCGCCGCACTCATCCGCGACGCCTGGCGCAACGATGATCTGACCGAGAACACCGAGAATCAGGTG CTGGGCGAATTCGGCGTCCTGCTCACCCGCGCCGACCACAAGGCCCGCGCCGACCGCTACAGCTACAAGCCCGATCAGGC CCGTGCGATGCGCGCCGCCGCCCGCGCGGGCTCGGACGTGGTCCTGCTGACGCAGGCCCGGCTCGCCATCGCGAAAAAGG CGCCGGAAGGCGCCCGCCTGCTGTCGCAGGTGCCCTTCACGCTCGCCAACGACCCGGCCTATCTCTTTGCCAAGTCCCAG CTCGCCCGCCGCGCCGGAAAGGACAGCGAGGCTGCCTCCGCACTGCTTCAGGCCGCGGGCGCCAGCCCCGACGTACTGGT GGACACGGATGAATGGTGGATCGAGCGCCGCCTTGTCTCGCGCGAGCTGCTGGATGCGGGCGACCCCAAGACCGCCTATC GCGTGGCCGCCACCGGCGCCATTCCGGACGCCTCCAACTATCGCGCGGAGCAGCAGTTCACCGCCGGCTGGATCGCCCTC GCCTTCCTGAAGGACCCGCGCACGGCGCAGGCGCATTTCTCGCGCATCGCCCACGGCCAGAGCAACCCGATCACGCTGTC CCGCGCCTTCTTCTGGCAGGGCCGCGCGGCGGAGGCGGCCGGCGACACTGGCTCGGCACGGTCGCACTATGAGCACGCGG CGCAGTACTCGACCACCTATTACGGGCAACTCGCCCGCCAGCGGCTGGGCCTGTCGGCCGTCGCCCTGCGCCGTGCGCCG GACGGTGGCATGCAGGCCCGCGCCACCTTCGACCGCATCGAGCCGGTGAAGGCGGTGCGCCTGCTCTATGCGCTGGGCGA CCGCGACACCGCCATGACCATTCTCTACGACCTCGCCTGGCGCATGGAGGACAGTTCCCACCTCCAGATGCTCTACCAGC TGGCACAGGAGAACAATGACGCGCGCGGCGCGCTGGTGGTGGGCAAGGAGAGCGTGGCCGAGGGCCATCCGCTGGACGCC GCCGCCTTCCCCACCTTCGGCATCCCGAACTACACCTCGATCGGTAATCCGGTGGACCGGGCCATGGTCTATGCGGTGGC CCGGCAGGAGAGCCAGTTCAACCCGCGCACCCTCTCCAGTGCCAAGGCCATGGGCCTGATGCAGGTGACGCCGGAGGCAG GCCGCGAGGTGACGAAGAAGACCGGCGTTGCCTATGACGAGGCCCGCCTGATGAACGACCAGTCCTACAACGTGCAGTTC GGCGCGGCGGAACTGGGCGAGCTGATGGAGAATTACGGCGGCAACACCGCCCTCGTCTTCGCCGCCTACAACGCCGGGCG CGGCTCCGTGCGCAAGTGGATCGACCGCTATGGCGACCCGCGCTCGCCGGATGTGGACCCCGTGGACTGGGTGGAACTCA TCCCCTATTCCGAGACGCGCAATTACGTGCAGCGGGTGATGGAGAATTATCTCGTCTACCGCGCCCGCTTCGGCTCGGAC CGCGCCCAACCGGTGGCCGCCCGCTGA
Upstream 100 bases:
>100_bases TTTCTGCCCGTATCGGTGCGGCAGGGTAGTTAGCGTTTCGTCAACGCGGCTCGCCCAAGATCGGCGCCGGATCCGCTTCG GCGCTGCGCCGTGAGGAAAA
Downstream 100 bases:
>100_bases GGACGGACGGCATCCACCCCATTGCTCCGAGGGCAATGTTCCCGTTGCATGCGCCCGCCCCCGGCGCCACCTTGCCGCGC CCGCGCTTCGCGGGCGCTGT
Product: lytic transglycosylase
Products: 1,6-Anhydrobond [C]
Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]
Number of amino acids: Translated: 728; Mature: 728
Protein sequence:
>728_residues MMPSVRLLLSCTALALALAVAPPAMAAQDSDAPTKKTDTKKADAKKADPKKDAKKDAKKPDAKSKAAAKPATHSPGRGVA ARPAPKAAPLTLAPTAAIATGELGELRNAVIAAKAGRGTEALQMARQLEDPVARNLVVWLVIRNAPNDYGFTNIAAFLRE KPGWPTPGTLRRRAERVLLAENKDPATVRAFFSEQDPISGEGKIALARAMAASGDRNTTAALIRDAWRNDDLTENTENQV LGEFGVLLTRADHKARADRYSYKPDQARAMRAAARAGSDVVLLTQARLAIAKKAPEGARLLSQVPFTLANDPAYLFAKSQ LARRAGKDSEAASALLQAAGASPDVLVDTDEWWIERRLVSRELLDAGDPKTAYRVAATGAIPDASNYRAEQQFTAGWIAL AFLKDPRTAQAHFSRIAHGQSNPITLSRAFFWQGRAAEAAGDTGSARSHYEHAAQYSTTYYGQLARQRLGLSAVALRRAP DGGMQARATFDRIEPVKAVRLLYALGDRDTAMTILYDLAWRMEDSSHLQMLYQLAQENNDARGALVVGKESVAEGHPLDA AAFPTFGIPNYTSIGNPVDRAMVYAVARQESQFNPRTLSSAKAMGLMQVTPEAGREVTKKTGVAYDEARLMNDQSYNVQF GAAELGELMENYGGNTALVFAAYNAGRGSVRKWIDRYGDPRSPDVDPVDWVELIPYSETRNYVQRVMENYLVYRARFGSD RAQPVAAR
Sequences:
>Translated_728_residues MMPSVRLLLSCTALALALAVAPPAMAAQDSDAPTKKTDTKKADAKKADPKKDAKKDAKKPDAKSKAAAKPATHSPGRGVA ARPAPKAAPLTLAPTAAIATGELGELRNAVIAAKAGRGTEALQMARQLEDPVARNLVVWLVIRNAPNDYGFTNIAAFLRE KPGWPTPGTLRRRAERVLLAENKDPATVRAFFSEQDPISGEGKIALARAMAASGDRNTTAALIRDAWRNDDLTENTENQV LGEFGVLLTRADHKARADRYSYKPDQARAMRAAARAGSDVVLLTQARLAIAKKAPEGARLLSQVPFTLANDPAYLFAKSQ LARRAGKDSEAASALLQAAGASPDVLVDTDEWWIERRLVSRELLDAGDPKTAYRVAATGAIPDASNYRAEQQFTAGWIAL AFLKDPRTAQAHFSRIAHGQSNPITLSRAFFWQGRAAEAAGDTGSARSHYEHAAQYSTTYYGQLARQRLGLSAVALRRAP DGGMQARATFDRIEPVKAVRLLYALGDRDTAMTILYDLAWRMEDSSHLQMLYQLAQENNDARGALVVGKESVAEGHPLDA AAFPTFGIPNYTSIGNPVDRAMVYAVARQESQFNPRTLSSAKAMGLMQVTPEAGREVTKKTGVAYDEARLMNDQSYNVQF GAAELGELMENYGGNTALVFAAYNAGRGSVRKWIDRYGDPRSPDVDPVDWVELIPYSETRNYVQRVMENYLVYRARFGSD RAQPVAAR >Mature_728_residues MMPSVRLLLSCTALALALAVAPPAMAAQDSDAPTKKTDTKKADAKKADPKKDAKKDAKKPDAKSKAAAKPATHSPGRGVA ARPAPKAAPLTLAPTAAIATGELGELRNAVIAAKAGRGTEALQMARQLEDPVARNLVVWLVIRNAPNDYGFTNIAAFLRE KPGWPTPGTLRRRAERVLLAENKDPATVRAFFSEQDPISGEGKIALARAMAASGDRNTTAALIRDAWRNDDLTENTENQV LGEFGVLLTRADHKARADRYSYKPDQARAMRAAARAGSDVVLLTQARLAIAKKAPEGARLLSQVPFTLANDPAYLFAKSQ LARRAGKDSEAASALLQAAGASPDVLVDTDEWWIERRLVSRELLDAGDPKTAYRVAATGAIPDASNYRAEQQFTAGWIAL AFLKDPRTAQAHFSRIAHGQSNPITLSRAFFWQGRAAEAAGDTGSARSHYEHAAQYSTTYYGQLARQRLGLSAVALRRAP DGGMQARATFDRIEPVKAVRLLYALGDRDTAMTILYDLAWRMEDSSHLQMLYQLAQENNDARGALVVGKESVAEGHPLDA AAFPTFGIPNYTSIGNPVDRAMVYAVARQESQFNPRTLSSAKAMGLMQVTPEAGREVTKKTGVAYDEARLMNDQSYNVQF GAAELGELMENYGGNTALVFAAYNAGRGSVRKWIDRYGDPRSPDVDPVDWVELIPYSETRNYVQRVMENYLVYRARFGSD RAQPVAAR
Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]
COG id: COG0741
COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)
Gene ontology:
Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the transglycosylase slt family [H]
Homologues:
Organism=Escherichia coli, GI87082441, Length=149, Percent_Identity=35.5704697986577, Blast_Score=85, Evalue=2e-17,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016026 - InterPro: IPR008258 - InterPro: IPR012289 - InterPro: IPR008939 - InterPro: IPR000189 [H]
Pfam domain/function: PF01464 SLT [H]
EC number: 3.2.1.- [C]
Molecular weight: Translated: 79027; Mature: 79027
Theoretical pI: Translated: 9.89; Mature: 9.89
Prosite motif: PS00922 TRANSGLYCOSYLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMPSVRLLLSCTALALALAVAPPAMAAQDSDAPTKKTDTKKADAKKADPKKDAKKDAKKP CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCC DAKSKAAAKPATHSPGRGVAARPAPKAAPLTLAPTAAIATGELGELRNAVIAAKAGRGTE CCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCCHHHHHHHHEEECCCCCHH ALQMARQLEDPVARNLVVWLVIRNAPNDYGFTNIAAFLREKPGWPTPGTLRRRAERVLLA HHHHHHHHHCHHHCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHEEEE ENKDPATVRAFFSEQDPISGEGKIALARAMAASGDRNTTAALIRDAWRNDDLTENTENQV CCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHH LGEFGVLLTRADHKARADRYSYKPDQARAMRAAARAGSDVVLLTQARLAIAKKAPEGARL HHHHHHHEECCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHHHH LSQVPFTLANDPAYLFAKSQLARRAGKDSEAASALLQAAGASPDVLVDTDEWWIERRLVS HHHCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH RELLDAGDPKTAYRVAATGAIPDASNYRAEQQFTAGWIALAFLKDPRTAQAHFSRIAHGQ HHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCC SNPITLSRAFFWQGRAAEAAGDTGSARSHYEHAAQYSTTYYGQLARQRLGLSAVALRRAP CCCEEEEEEHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC DGGMQARATFDRIEPVKAVRLLYALGDRDTAMTILYDLAWRMEDSSHLQMLYQLAQENND CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC ARGALVVGKESVAEGHPLDAAAFPTFGIPNYTSIGNPVDRAMVYAVARQESQFNPRTLSS CCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH AKAMGLMQVTPEAGREVTKKTGVAYDEARLMNDQSYNVQFGAAELGELMENYGGNTALVF HHHCCEEEECCHHHHHHHHHHCCCHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCEEEEE AAYNAGRGSVRKWIDRYGDPRSPDVDPVDWVELIPYSETRNYVQRVMENYLVYRARFGSD EEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC RAQPVAAR CCCCCCCC >Mature Secondary Structure MMPSVRLLLSCTALALALAVAPPAMAAQDSDAPTKKTDTKKADAKKADPKKDAKKDAKKP CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCC DAKSKAAAKPATHSPGRGVAARPAPKAAPLTLAPTAAIATGELGELRNAVIAAKAGRGTE CCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCCHHHHHHHHEEECCCCCHH ALQMARQLEDPVARNLVVWLVIRNAPNDYGFTNIAAFLREKPGWPTPGTLRRRAERVLLA HHHHHHHHHCHHHCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHEEEE ENKDPATVRAFFSEQDPISGEGKIALARAMAASGDRNTTAALIRDAWRNDDLTENTENQV CCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHH LGEFGVLLTRADHKARADRYSYKPDQARAMRAAARAGSDVVLLTQARLAIAKKAPEGARL HHHHHHHEECCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHHHH LSQVPFTLANDPAYLFAKSQLARRAGKDSEAASALLQAAGASPDVLVDTDEWWIERRLVS HHHCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH RELLDAGDPKTAYRVAATGAIPDASNYRAEQQFTAGWIALAFLKDPRTAQAHFSRIAHGQ HHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCC SNPITLSRAFFWQGRAAEAAGDTGSARSHYEHAAQYSTTYYGQLARQRLGLSAVALRRAP CCCEEEEEEHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC DGGMQARATFDRIEPVKAVRLLYALGDRDTAMTILYDLAWRMEDSSHLQMLYQLAQENND CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC ARGALVVGKESVAEGHPLDAAAFPTFGIPNYTSIGNPVDRAMVYAVARQESQFNPRTLSS CCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH AKAMGLMQVTPEAGREVTKKTGVAYDEARLMNDQSYNVQFGAAELGELMENYGGNTALVF HHHCCEEEECCHHHHHHHHHHCCCHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCEEEEE AAYNAGRGSVRKWIDRYGDPRSPDVDPVDWVELIPYSETRNYVQRVMENYLVYRARFGSD EEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC RAQPVAAR CCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]
Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]
General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]