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The map label for this gene is nifH [H]

Identifier: 158422665

GI number: 158422665

Start: 1169826

End: 1170716

Strand: Reverse

Name: nifH [H]

Synonym: AZC_1041

Alternate gene names: 158422665

Gene position: 1170716-1169826 (Counterclockwise)

Preceding gene: 158422672

Following gene: 158422664

Centisome position: 21.8

GC content: 64.2

Gene sequence:

>891_bases
ATGTCGTCACTGAGACAGATCGCATTTTACGGCAAGGGGGGCATCGGCAAGTCGACGACGTCCCAGAACACGCTGGCGGC
GCTGGCGGAGATGGGCCATCGCATCCTCATCGTCGGCTGCGACCCCAAGGCCGACTCAACCCGCCTCATCCTGCACGCCA
AGGCGCAGGACACCATCCTGTCGCTGGCCGCCGCCGCCGGCAGCGTCGAAGACCTCGAGCTCGAAGAGGTCATGAAGATC
GGCTACCGCGACATCCGCTGCGTGGAATCCGGCGGTCCGGAGCCGGGCGTCGGCTGCGCGGGGCGCGGCGTCATCACCTC
CATCAATTTCCTGGAGGAGAACGGCGCCTATGAGGACATCGACTACGTCTCCTACGACGTGCTCGGCGACGTGGTGTGCG
GCGGCTTCGCCATGCCCATCCGCGAGAACAAGGCGCAGGAAATCTACATCGTCATGTCCGGCGAGATGATGGCCATGTAT
GCGGCCAACAACATCTCCAAGGGCATTCTCAAGTACGCCAATTCCGGCGGCGTGCGCCTGGGCGGGCTCGTCTGCAACGA
GCGCCAGACGGACAAGGAGCTGGAACTGGCCGAGAACCTCGCCAAGAAGCTCGGCACCCAGCTCATCTATTTCGTGCCGC
GCGACAACATCGTGCAGCACGCCGAGCTGCGCCGCATGACGGTGATCGAGTATGCGCCCGACAGCGTTCAGGCGAACCAC
TATCGCAACCTCGCTGAGCGCGTCCACAACAATGGCGGCAAGGGCATCATCCCGACCCCCATCACCATGGACGAGCTCGA
GGACATGCTCATGGAGCACGGCATCATGAAGACCGTGGACGAGAGCCAGGTCGGCAAGACCGCGGCCGAGCTCGCCGCGC
TCTCCGCCTGA

Upstream 100 bases:

>100_bases
AGTCCGGTCCCGATCCAGTTCGGCGGGGCCACACCTGGCTGAAGGCGCAGGCCGCCCCAGCGGAGCTGCCACGGTTCCCA
AGGTTCCAAGGAGACCAGAC

Downstream 100 bases:

>100_bases
GCCGACACGCCGGCCGGCCCCCGCGTGGGGCCGGCCTTCGCAACGCAATGAAGCGAGGAACTCCAAAATGAGCGTGTCAC
AGCCTCAGACCGTTGAGGAA

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein 1; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTILSLAAAAGSVEDLELEEVMKI
GYRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMY
AANNISKGILKYANSGGVRLGGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANH
YRNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDESQVGKTAAELAALSA

Sequences:

>Translated_296_residues
MSSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTILSLAAAAGSVEDLELEEVMKI
GYRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMY
AANNISKGILKYANSGGVRLGGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANH
YRNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDESQVGKTAAELAALSA
>Mature_295_residues
SSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTILSLAAAAGSVEDLELEEVMKIG
YRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYA
ANNISKGILKYANSGGVRLGGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANHY
RNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDESQVGKTAAELAALSA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31988; Mature: 31857

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTIL
CCCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCHHHHH
SLAAAAGSVEDLELEEVMKIGYRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDI
HHHHHCCCCCCCCHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCC
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEE
GGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANH
EEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHEEEEEECCCCCCHHH
YRNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDESQVGKTAAELAALSA
HHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSLRQIAFYGKGGIGKSTTSQNTLAALAEMGHRILIVGCDPKADSTRLILHAKAQDTIL
CCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCHHHHH
SLAAAAGSVEDLELEEVMKIGYRDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYEDI
HHHHHCCCCCCCCHHHHHHCCCHHEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCC
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEE
GGLVCNERQTDKELELAENLAKKLGTQLIYFVPRDNIVQHAELRRMTVIEYAPDSVQANH
EEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHEEEEEECCCCCCHHH
YRNLAERVHNNGGKGIIPTPITMDELEDMLMEHGIMKTVDESQVGKTAAELAALSA
HHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA