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The map label for this gene is trpA

Identifier: 158422644

GI number: 158422644

Start: 1144667

End: 1145506

Strand: Direct

Name: trpA

Synonym: AZC_1020

Alternate gene names: 158422644

Gene position: 1144667-1145506 (Clockwise)

Preceding gene: 158422643

Following gene: 158422645

Centisome position: 21.32

GC content: 70.24

Gene sequence:

>840_bases
ATGACCACCCGCATCACCGCCCGCTTCACGGAACTCGCCGCCGAAGGCCGCCCCGGCCTCGTGACCTTCATCACGGCGGG
CGATCCCGATTACGACACCGCGCTTTCCATCCTGCGCGGACTGCCGGAGGCGGGGGCCGACGTGATCGAGGTCGGCATCC
CCTTCACCGATCCCATGGCCGACGGCCCGGCCATCCAGGCCGCCGGTCTGCGGGCACTGAAGGGCGGGCAGACGCTCGCC
AAGACGCTCGACATGGTGCGGGCGTTCCGGGACGAGAATGACACCACGCCCGTGGTGCTCATGGGCTACTACAACCCCAT
CTACATCTATGGCGTGCCGCGCTTCATCGCCGACGCCAAGGCGGCGGGCGTGGACGGCCTCATCGTCGTGGACCTGCCGC
CGGAAGAGGATAACGAGCTCTGCCTGCCGGCGCTCGACGCGGGGCTGGACTTCATCCGCCTCGCCACCCCGACCACGGAC
GAGAAGCGCCTGCCGGCCGTGCTCGCCCATACGGCGGGCTTTGTCTATTACGTCTCCATCACCGGCATCACCGGCTCGGC
GACCCCCGACGCGAATGCGGTGGCGGGCGCGGTGGCGCGCATCAAGCAGCACACCACGCTGCCCGTGGCAGTCGGCTTCG
GCGTGAAGACGCCGGCCCAGGCGGCGGCGATCGCGGCGGGTGCGGATGGCGTCGTGGTGGGCTCGGCCCTCGTCGAGGCG
GTGCGCAAGACGCTGGATGAGGACGGCAAGGCGACCGTGAACACGGTTCCGGCCGTGACGGCGCTGGTGCAGGAACTGGC
GGAAGCGGTGCGCGGCGTAAATCGCGCGGCGGCGGAATAG

Upstream 100 bases:

>100_bases
ACGCCGCCCGCCTGATCCTGCGGCGGGCCGCGCGCCCGCTCCTCGCGCATCCGCACACCCCCGGCCGCCTGCGGACCGGA
GGGGAGAGACAGACGGACCC

Downstream 100 bases:

>100_bases
TCCACCCCTTCTCCTCGCGCGTCCCTCGGCCGCGCGACCTTTGGGCCGCTTGCCCCGCGCCCGTGTGCGGCCTTCACTCC
CCTTGCGGCATCGGAGGGGG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTTRITARFTELAAEGRPGLVTFITAGDPDYDTALSILRGLPEAGADVIEVGIPFTDPMADGPAIQAAGLRALKGGQTLA
KTLDMVRAFRDENDTTPVVLMGYYNPIYIYGVPRFIADAKAAGVDGLIVVDLPPEEDNELCLPALDAGLDFIRLATPTTD
EKRLPAVLAHTAGFVYYVSITGITGSATPDANAVAGAVARIKQHTTLPVAVGFGVKTPAQAAAIAAGADGVVVGSALVEA
VRKTLDEDGKATVNTVPAVTALVQELAEAVRGVNRAAAE

Sequences:

>Translated_279_residues
MTTRITARFTELAAEGRPGLVTFITAGDPDYDTALSILRGLPEAGADVIEVGIPFTDPMADGPAIQAAGLRALKGGQTLA
KTLDMVRAFRDENDTTPVVLMGYYNPIYIYGVPRFIADAKAAGVDGLIVVDLPPEEDNELCLPALDAGLDFIRLATPTTD
EKRLPAVLAHTAGFVYYVSITGITGSATPDANAVAGAVARIKQHTTLPVAVGFGVKTPAQAAAIAAGADGVVVGSALVEA
VRKTLDEDGKATVNTVPAVTALVQELAEAVRGVNRAAAE
>Mature_278_residues
TTRITARFTELAAEGRPGLVTFITAGDPDYDTALSILRGLPEAGADVIEVGIPFTDPMADGPAIQAAGLRALKGGQTLAK
TLDMVRAFRDENDTTPVVLMGYYNPIYIYGVPRFIADAKAAGVDGLIVVDLPPEEDNELCLPALDAGLDFIRLATPTTDE
KRLPAVLAHTAGFVYYVSITGITGSATPDANAVAGAVARIKQHTTLPVAVGFGVKTPAQAAAIAAGADGVVVGSALVEAV
RKTLDEDGKATVNTVPAVTALVQELAEAVRGVNRAAAE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=270, Percent_Identity=32.962962962963, Blast_Score=147, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6321412, Length=240, Percent_Identity=45, Blast_Score=199, Evalue=5e-52,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_AZOC5 (A8HQ60)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523936.1
- ProteinModelPortal:   A8HQ60
- SMR:   A8HQ60
- GeneID:   5691983
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1020
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   ACAU438753:AZC_1020-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28795; Mature: 28664

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 50-50 ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRITARFTELAAEGRPGLVTFITAGDPDYDTALSILRGLPEAGADVIEVGIPFTDPMA
CCCEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
DGPAIQAAGLRALKGGQTLAKTLDMVRAFRDENDTTPVVLMGYYNPIYIYGVPRFIADAK
CCCCCHHHCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECHHHHHHHH
AAGVDGLIVVDLPPEEDNELCLPALDAGLDFIRLATPTTDEKRLPAVLAHTAGFVYYVSI
HCCCCCEEEEECCCCCCCCEECHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEEE
TGITGSATPDANAVAGAVARIKQHTTLPVAVGFGVKTPAQAAAIAAGADGVVVGSALVEA
ECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCCEEHHHHHHHH
VRKTLDEDGKATVNTVPAVTALVQELAEAVRGVNRAAAE
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTRITARFTELAAEGRPGLVTFITAGDPDYDTALSILRGLPEAGADVIEVGIPFTDPMA
CCEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
DGPAIQAAGLRALKGGQTLAKTLDMVRAFRDENDTTPVVLMGYYNPIYIYGVPRFIADAK
CCCCCHHHCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECHHHHHHHH
AAGVDGLIVVDLPPEEDNELCLPALDAGLDFIRLATPTTDEKRLPAVLAHTAGFVYYVSI
HCCCCCEEEEECCCCCCCCEECHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEEE
TGITGSATPDANAVAGAVARIKQHTTLPVAVGFGVKTPAQAAAIAAGADGVVVGSALVEA
ECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCCEEHHHHHHHH
VRKTLDEDGKATVNTVPAVTALVQELAEAVRGVNRAAAE
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA