Click here to switch to the map view.

The map label for this gene is yogA [H]

Identifier: 158422621

GI number: 158422621

Start: 1114436

End: 1115797

Strand: Direct

Name: yogA [H]

Synonym: AZC_0997

Alternate gene names: 158422621

Gene position: 1114436-1115797 (Clockwise)

Preceding gene: 158422620

Following gene: 158422623

Centisome position: 20.75

GC content: 66.3

Gene sequence:

>1362_bases
ATGTGCAACCGCGGTCTGCCCGGTTGCGTCGGAAACGAGACCGAATGGTCCGCATGGAGAGAGGATCGCGACATGGGCCA
GCCCGCCACCTTGCCGAGCTCCGAAGCGCCGCTGAAGGATCTCTATGAGATCGGCGAAATTCCGCCGCTTGGGCACGTTC
CCAGGCAGATGTACGCTTGGACCATCCGCCGCGAGCGGCACGGCCCGCCGGAGGACTCCTTCCAGATCGAGGTGGTGCCC
ACCTGGGAACTGGGCGAGGCGGATGTGCTGGTGCTCGTGATGGCGGCCGGCGTGAATTACAACGGCATCTGGGCGGGCCT
CGGCCAGCCGATCTCCCCCTTCGACGTGCACAAGCAGCCGTATCATATCGCCGGCTCCGACGCCTCCGGCATCGTCTGGG
CGGTGGGCTCCAAGGTCACCCGCTGGAAGGTGGGCGACGAAGTGGTCGTCCACTGCAATCAGGACGACGGCGACGACGAG
GAGTGCAACGGCGGCGACCCGATGTTCTCCCCCTCCCAGCGCATCTGGGGCTATGAGACGCCGGATGGCTCCTTCGCTCA
GTTCTGCCGCGTGCAGGCGCGCCAGCTCATGCCGCGCCCCAAGCATCTGACTTGGGAGGAGTGCGCCTGCTACACGCTGA
CGCTCGCTACCGCCTATCGCATGCTGTTCGGCCACGCCCCCCACGACATCAAGCCCGGCCAGTTCGTGCTGGTCTGGGGC
GCCTCGGGCGGCCTCGGTGTGTTCGGCGTGCAACTGGCGGCGGCCTCGGGCGCCCACGTGATCGGCGTGATCTCGGACGA
GACCAAGCGCGACTACGTGCTTCAGCTCGGCGCGCGCGGCGTCATCAACCGCAAGGATTTCAAGTGCTGGGGCCAGATGC
CCAAGGTCAATTCGCCCGAATACAATGAGTGGGTGAAGGAAGCCCGCAAGTTCGGCAAGGCGATCTGGGACATCACCGGC
AAGCGCGACGTGGACATGGTGTTCGAGCATCCGGGCGAGCAGACCTTCCCGGTCTCCTGCCTCGTCGCCAAGCGCGGCGG
CATGGTGGTGTTCTGCGCCGGCACCACGGGCTTCAACATCACCTTCGATGCCCGCTATGTGTGGATGCGGCAGAAGCGCA
TCCAGGGCTCGCACTTCGCGCACCTGAAGCAGGCGTCCTCCGCGAACCAGTTCATCATCGACCGGCGGGTCGACCCCTGC
ATGTCGGAAGTGTTCCCCTGGGCGAAGATCCCGGCGGCGCACACGCTGATGTGGAAGAACCAGCACCCGCCCGGCAACAT
GTCGGTGCTGGTGAACGCCCCGCGGGCCGGTCTGCGCTCGTTCGAGGACGTTCTGGAAGCCTCCGGCGCCCCGGCTCTCT
GA

Upstream 100 bases:

>100_bases
CGTGATCCGCGCTGGCCTGCCAGTTTCCGCCGCCTTTCTTATCCGAAAGAGGGCTCGCCAGCCCGATTTTCTCTGCTACG
CTGCGGTGCGAAGATAAGAA

Downstream 100 bases:

>100_bases
CGGCCTCAGGCAGGGATCGGGTTCGGTGCCGTGGCTGCTTCGGCGGTGCGGTTCCGGCCTTCCGCCTTGGCGCTGTAGAG
CGCGCGGTCGATCCGTTGCA

Product: crotonyl-CoA reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MCNRGLPGCVGNETEWSAWREDRDMGQPATLPSSEAPLKDLYEIGEIPPLGHVPRQMYAWTIRRERHGPPEDSFQIEVVP
TWELGEADVLVLVMAAGVNYNGIWAGLGQPISPFDVHKQPYHIAGSDASGIVWAVGSKVTRWKVGDEVVVHCNQDDGDDE
ECNGGDPMFSPSQRIWGYETPDGSFAQFCRVQARQLMPRPKHLTWEECACYTLTLATAYRMLFGHAPHDIKPGQFVLVWG
ASGGLGVFGVQLAAASGAHVIGVISDETKRDYVLQLGARGVINRKDFKCWGQMPKVNSPEYNEWVKEARKFGKAIWDITG
KRDVDMVFEHPGEQTFPVSCLVAKRGGMVVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASSANQFIIDRRVDPC
MSEVFPWAKIPAAHTLMWKNQHPPGNMSVLVNAPRAGLRSFEDVLEASGAPAL

Sequences:

>Translated_453_residues
MCNRGLPGCVGNETEWSAWREDRDMGQPATLPSSEAPLKDLYEIGEIPPLGHVPRQMYAWTIRRERHGPPEDSFQIEVVP
TWELGEADVLVLVMAAGVNYNGIWAGLGQPISPFDVHKQPYHIAGSDASGIVWAVGSKVTRWKVGDEVVVHCNQDDGDDE
ECNGGDPMFSPSQRIWGYETPDGSFAQFCRVQARQLMPRPKHLTWEECACYTLTLATAYRMLFGHAPHDIKPGQFVLVWG
ASGGLGVFGVQLAAASGAHVIGVISDETKRDYVLQLGARGVINRKDFKCWGQMPKVNSPEYNEWVKEARKFGKAIWDITG
KRDVDMVFEHPGEQTFPVSCLVAKRGGMVVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASSANQFIIDRRVDPC
MSEVFPWAKIPAAHTLMWKNQHPPGNMSVLVNAPRAGLRSFEDVLEASGAPAL
>Mature_453_residues
MCNRGLPGCVGNETEWSAWREDRDMGQPATLPSSEAPLKDLYEIGEIPPLGHVPRQMYAWTIRRERHGPPEDSFQIEVVP
TWELGEADVLVLVMAAGVNYNGIWAGLGQPISPFDVHKQPYHIAGSDASGIVWAVGSKVTRWKVGDEVVVHCNQDDGDDE
ECNGGDPMFSPSQRIWGYETPDGSFAQFCRVQARQLMPRPKHLTWEECACYTLTLATAYRMLFGHAPHDIKPGQFVLVWG
ASGGLGVFGVQLAAASGAHVIGVISDETKRDYVLQLGARGVINRKDFKCWGQMPKVNSPEYNEWVKEARKFGKAIWDITG
KRDVDMVFEHPGEQTFPVSCLVAKRGGMVVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASSANQFIIDRRVDPC
MSEVFPWAKIPAAHTLMWKNQHPPGNMSVLVNAPRAGLRSFEDVLEASGAPAL

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=353, Percent_Identity=23.7960339943343, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI13236495, Length=353, Percent_Identity=23.7960339943343, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI194239676, Length=215, Percent_Identity=27.4418604651163, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988145, Length=233, Percent_Identity=28.3261802575107, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=296, Percent_Identity=26.6891891891892, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=162, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319621, Length=202, Percent_Identity=32.1782178217822, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=162, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323961, Length=162, Percent_Identity=29.0123456790123, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 50294; Mature: 50294

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNRGLPGCVGNETEWSAWREDRDMGQPATLPSSEAPLKDLYEIGEIPPLGHVPRQMYAW
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHH
TIRRERHGPPEDSFQIEVVPTWELGEADVLVLVMAAGVNYNGIWAGLGQPISPFDVHKQP
HHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCHHCCCCC
YHIAGSDASGIVWAVGSKVTRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYET
EEEECCCCCCEEEECCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEC
PDGSFAQFCRVQARQLMPRPKHLTWEECACYTLTLATAYRMLFGHAPHDIKPGQFVLVWG
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
ASGGLGVFGVQLAAASGAHVIGVISDETKRDYVLQLGARGVINRKDFKCWGQMPKVNSPE
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCHHHCCCCCCCCCC
YNEWVKEARKFGKAIWDITGKRDVDMVFEHPGEQTFPVSCLVAKRGGMVVFCAGTTGFNI
HHHHHHHHHHHHHHHHCCCCCCCCCHHEECCCCCCCCEEEEEECCCCEEEEEECCCCEEE
TFDARYVWMRQKRIQGSHFAHLKQASSANQFIIDRRVDPCMSEVFPWAKIPAAHTLMWKN
EEEEEEEEEHHHHCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCEEEEECC
QHPPGNMSVLVNAPRAGLRSFEDVLEASGAPAL
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCNRGLPGCVGNETEWSAWREDRDMGQPATLPSSEAPLKDLYEIGEIPPLGHVPRQMYAW
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHH
TIRRERHGPPEDSFQIEVVPTWELGEADVLVLVMAAGVNYNGIWAGLGQPISPFDVHKQP
HHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCHHCCCCC
YHIAGSDASGIVWAVGSKVTRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYET
EEEECCCCCCEEEECCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEC
PDGSFAQFCRVQARQLMPRPKHLTWEECACYTLTLATAYRMLFGHAPHDIKPGQFVLVWG
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
ASGGLGVFGVQLAAASGAHVIGVISDETKRDYVLQLGARGVINRKDFKCWGQMPKVNSPE
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCHHHCCCCCCCCCC
YNEWVKEARKFGKAIWDITGKRDVDMVFEHPGEQTFPVSCLVAKRGGMVVFCAGTTGFNI
HHHHHHHHHHHHHHHHCCCCCCCCCHHEECCCCCCCCEEEEEECCCCEEEEEECCCCEEE
TFDARYVWMRQKRIQGSHFAHLKQASSANQFIIDRRVDPCMSEVFPWAKIPAAHTLMWKN
EEEEEEEEEHHHHCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCEEEEECC
QHPPGNMSVLVNAPRAGLRSFEDVLEASGAPAL
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]