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The map label for this gene is nylB [H]

Identifier: 158422598

GI number: 158422598

Start: 1083007

End: 1084263

Strand: Reverse

Name: nylB [H]

Synonym: AZC_0974

Alternate gene names: 158422598

Gene position: 1084263-1083007 (Counterclockwise)

Preceding gene: 158422599

Following gene: 158422597

Centisome position: 20.19

GC content: 68.18

Gene sequence:

>1257_bases
GTGCGCGCGATGCGGTTTGGACGGCGGGCGCTCCTTCTGATCTCGATGCTGGCGGCAGCCTGCCCTGCCGCCGCCTCTCC
CGATGAGGATGTGCTGGGAAAGGCGCAAGGCTATCCTGTCGCCCCCAGCCTGGGGCAGACGCGGCAGGAGCCCTATATCG
TCGGCTCCTTCAGCGGCATGGAGAAGATTGCCGAGACCTGCCGGATGTCACCCGCGGCGGTTCCCCGTCCCCTGCCGGCC
GCCCCGGCCGGCGCGCCGCTGACCTATCGCTACAAAGGCGCCGACTACACCCTCGACGATTATATGGCGCACCAGCGCAT
CACCGGCCTCATCGTCCTGCATGACGGGGCGATCGTTGCCGAGCGCTACGGCTATGACCGCACGCCGGACATGCGGTTCC
TCTCCAATTCCATGTCCAAGACGGTGACCGCGCTCGCCATCCTGAAGGCGCAGGAGGAAGGCCGCATCCGCTCGCTGGAC
GACCGCGCGCAGGACTATGTGCCGGAACTCTCCGGCTCGCTTTATGGCGGGACGCGGCTGGTCGATCTCATGCGCATGGC
ATCCGGTGCCCGCTTCACCGAGACCTACACGCCGGGCGACGACCGCGCCGCCTTCAACCGGATCATGTCCCGGACGGGAA
CGGTGGCGGCCCTGAAGAGCGTGACCGAGCGCGCCGTCCCGGCCGGAGAAGCCTTCAATTATGCCGGCGCGCAGACGCAG
GCGCTCGGCGTAGTGCTGCGCGCGGCCACCGGAGACACCCTTTGCCATTATGTGGAACGCACCCTCTGGCAGCCCATGGG
CGCGGAATCCCCGGCAAGCTGGATCCTCAACCGGTCCGATCACGTGGAGATCGCACAGGGCGGGCTGAATGCGACCCTGC
ATGATTATGCCCGCCTCGGCCTGCTGCTGGCGGATGACGGCATGGTGGCCGGGCAGCCCGTGCTCTCGCGGGAGCACTTA
TTGGACATGACGGCGGCCGAGCGCCAGCCGGAGGCCTTCCGGCCGGGCCGGATGAACTGGCACGGCAGCACATATGTCGG
CTACGGCCTCCAGGTGTGGCTCTGGCCCGGCAGCCACCGGCGCTTCGCTCTTCTGGGCATTCACGGCCAGTCGATCTTCG
TCGATCCCGATCTCAAGCTCGTCATCGTGCAGACCGCCGTCGGCAAGGACGCCGCCGGCGACGCGAGCGGCGCGCATCTC
GGTGCCGAGCGGGGCGCGCTCTTCCGCGGCATTGTTGCGCGCTACGGGAGCTGGTGA

Upstream 100 bases:

>100_bases
CGCGTGATGGGCGAGGGCGATGATTTCGAGCTGGTGGAAGGCGTGGTGGACGAAGTGATCGAGGCGCTGCGCAAGGTCGC
GGCCTGACGAGCGCGGAGGA

Downstream 100 bases:

>100_bases
ATGGCGCCACACCTTGATGATAGAGGTACAAAGGTATTTCGAGCAAAAATGCCGGTGTTCAGGCGAGGACCCATGAACCG
CGCGCCTTGCTGCGAGAAGG

Product: beta-lactamase class C

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MRAMRFGRRALLLISMLAAACPAAASPDEDVLGKAQGYPVAPSLGQTRQEPYIVGSFSGMEKIAETCRMSPAAVPRPLPA
APAGAPLTYRYKGADYTLDDYMAHQRITGLIVLHDGAIVAERYGYDRTPDMRFLSNSMSKTVTALAILKAQEEGRIRSLD
DRAQDYVPELSGSLYGGTRLVDLMRMASGARFTETYTPGDDRAAFNRIMSRTGTVAALKSVTERAVPAGEAFNYAGAQTQ
ALGVVLRAATGDTLCHYVERTLWQPMGAESPASWILNRSDHVEIAQGGLNATLHDYARLGLLLADDGMVAGQPVLSREHL
LDMTAAERQPEAFRPGRMNWHGSTYVGYGLQVWLWPGSHRRFALLGIHGQSIFVDPDLKLVIVQTAVGKDAAGDASGAHL
GAERGALFRGIVARYGSW

Sequences:

>Translated_418_residues
MRAMRFGRRALLLISMLAAACPAAASPDEDVLGKAQGYPVAPSLGQTRQEPYIVGSFSGMEKIAETCRMSPAAVPRPLPA
APAGAPLTYRYKGADYTLDDYMAHQRITGLIVLHDGAIVAERYGYDRTPDMRFLSNSMSKTVTALAILKAQEEGRIRSLD
DRAQDYVPELSGSLYGGTRLVDLMRMASGARFTETYTPGDDRAAFNRIMSRTGTVAALKSVTERAVPAGEAFNYAGAQTQ
ALGVVLRAATGDTLCHYVERTLWQPMGAESPASWILNRSDHVEIAQGGLNATLHDYARLGLLLADDGMVAGQPVLSREHL
LDMTAAERQPEAFRPGRMNWHGSTYVGYGLQVWLWPGSHRRFALLGIHGQSIFVDPDLKLVIVQTAVGKDAAGDASGAHL
GAERGALFRGIVARYGSW
>Mature_418_residues
MRAMRFGRRALLLISMLAAACPAAASPDEDVLGKAQGYPVAPSLGQTRQEPYIVGSFSGMEKIAETCRMSPAAVPRPLPA
APAGAPLTYRYKGADYTLDDYMAHQRITGLIVLHDGAIVAERYGYDRTPDMRFLSNSMSKTVTALAILKAQEEGRIRSLD
DRAQDYVPELSGSLYGGTRLVDLMRMASGARFTETYTPGDDRAAFNRIMSRTGTVAALKSVTERAVPAGEAFNYAGAQTQ
ALGVVLRAATGDTLCHYVERTLWQPMGAESPASWILNRSDHVEIAQGGLNATLHDYARLGLLLADDGMVAGQPVLSREHL
LDMTAAERQPEAFRPGRMNWHGSTYVGYGLQVWLWPGSHRRFALLGIHGQSIFVDPDLKLVIVQTAVGKDAAGDASGAHL
GAERGALFRGIVARYGSW

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 45214; Mature: 45214

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMRFGRRALLLISMLAAACPAAASPDEDVLGKAQGYPVAPSLGQTRQEPYIVGSFSGM
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEECHHHH
EKIAETCRMSPAAVPRPLPAAPAGAPLTYRYKGADYTLDDYMAHQRITGLIVLHDGAIVA
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEECCEEEE
ERYGYDRTPDMRFLSNSMSKTVTALAILKAQEEGRIRSLDDRAQDYVPELSGSLYGGTRL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
VDLMRMASGARFTETYTPGDDRAAFNRIMSRTGTVAALKSVTERAVPAGEAFNYAGAQTQ
HHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHH
ALGVVLRAATGDTLCHYVERTLWQPMGAESPASWILNRSDHVEIAQGGLNATLHDYARLG
HHEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEECCCCCCHHHHHHHCC
LLLADDGMVAGQPVLSREHLLDMTAAERQPEAFRPGRMNWHGSTYVGYGLQVWLWPGSHR
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCC
RFALLGIHGQSIFVDPDLKLVIVQTAVGKDAAGDASGAHLGAERGALFRGIVARYGSW
EEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRAMRFGRRALLLISMLAAACPAAASPDEDVLGKAQGYPVAPSLGQTRQEPYIVGSFSGM
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEECHHHH
EKIAETCRMSPAAVPRPLPAAPAGAPLTYRYKGADYTLDDYMAHQRITGLIVLHDGAIVA
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEECCEEEE
ERYGYDRTPDMRFLSNSMSKTVTALAILKAQEEGRIRSLDDRAQDYVPELSGSLYGGTRL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
VDLMRMASGARFTETYTPGDDRAAFNRIMSRTGTVAALKSVTERAVPAGEAFNYAGAQTQ
HHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHH
ALGVVLRAATGDTLCHYVERTLWQPMGAESPASWILNRSDHVEIAQGGLNATLHDYARLG
HHEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEECCCCCCHHHHHHHCC
LLLADDGMVAGQPVLSREHLLDMTAAERQPEAFRPGRMNWHGSTYVGYGLQVWLWPGSHR
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCC
RFALLGIHGQSIFVDPDLKLVIVQTAVGKDAAGDASGAHLGAERGALFRGIVARYGSW
EEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]