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The map label for this gene is ppnK [H]

Identifier: 158422577

GI number: 158422577

Start: 1066273

End: 1067055

Strand: Reverse

Name: ppnK [H]

Synonym: AZC_0953

Alternate gene names: 158422577

Gene position: 1067055-1066273 (Counterclockwise)

Preceding gene: 158422579

Following gene: 158422576

Centisome position: 19.87

GC content: 66.92

Gene sequence:

>783_bases
ATGAATGACAGCCCACGGCGCTTCGAGCGCATCGCCTTCATGGCTAGCGGCACGCCCGAGGCCGAGGCCGCGCGCGCGCG
GCTCATCCAGCGCTATGGCGACGTGAGCGAAGACGCGGCGGACGTGATCGTCGCTCTCGGCGGCGACGGCCTGATGCTCC
AGACCCTGCATCACTTTCGCGACAAGGGCGTGCCCATCTATGGCATGCATCGTGGCTCCGTCGGCTTCCTCATGAACACG
TATCGCGAGGACGACCTGCGTGCCCGCCTCGCCAATGCCACCCATGTGGTGATCCATCCGCTGATGATGGAGGCCGTGGA
TGCGGCCGGCGTGCGCTACAGCGCCCCGGCCATCAACGAGGTGTCGCTGCTGCGCCAGACGTATCAGGCCGCCAAGCTGC
GCATCTCCATCGACGGCAAGGTGCGGCTGGAGGAACTCATCTGCGACGGCGTGCTGGTGGCGACCCCCGCCGGCTCCACC
GCCTACAACCTCTCCGCCCACGGCCCCATCCTGCCGCTGGGGGCGGCGCTACTGGCGCTCACTCCCATTTCCCCCTTCCG
GCCCCGCCGCTGGCGCGGGGCGCTGGTGCCCGACCGGGCCACCATCCAGATCGAGATCCTGGAAGCCGACAAGCGGCCGG
TGAGCGCGGTGGCTGATCACAAGGAAGTGCGTGACATCATCGAGGTCACCGCGCGCCTCGACAAGACCTCATCCATGGAC
ATGCTGTTCGACCCCGATCATGGGCTGGACGAACGCATCCTGCGCGAACAGTTCATCTATTAG

Upstream 100 bases:

>100_bases
CTCCGTCTGCCGCTGAGCGGTGTGGGAACCGGGGACGGCGCGCGACCGCGTATCCATATGCGCCGCCTCCCCCGAGTGGC
AGCTGAGGCAACTGTCCGGA

Downstream 100 bases:

>100_bases
GCGTATGCCGAGTCCCTCCGAATGATCTTCTCGTCGGTCACGTTCCTCTACTATTTCCTCCCCGCCTTCCTGATCTGCTA
TTTCGCGGTGCCGGGCGTCC

Product: sugar kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNDSPRRFERIAFMASGTPEAEAARARLIQRYGDVSEDAADVIVALGGDGLMLQTLHHFRDKGVPIYGMHRGSVGFLMNT
YREDDLRARLANATHVVIHPLMMEAVDAAGVRYSAPAINEVSLLRQTYQAAKLRISIDGKVRLEELICDGVLVATPAGST
AYNLSAHGPILPLGAALLALTPISPFRPRRWRGALVPDRATIQIEILEADKRPVSAVADHKEVRDIIEVTARLDKTSSMD
MLFDPDHGLDERILREQFIY

Sequences:

>Translated_260_residues
MNDSPRRFERIAFMASGTPEAEAARARLIQRYGDVSEDAADVIVALGGDGLMLQTLHHFRDKGVPIYGMHRGSVGFLMNT
YREDDLRARLANATHVVIHPLMMEAVDAAGVRYSAPAINEVSLLRQTYQAAKLRISIDGKVRLEELICDGVLVATPAGST
AYNLSAHGPILPLGAALLALTPISPFRPRRWRGALVPDRATIQIEILEADKRPVSAVADHKEVRDIIEVTARLDKTSSMD
MLFDPDHGLDERILREQFIY
>Mature_260_residues
MNDSPRRFERIAFMASGTPEAEAARARLIQRYGDVSEDAADVIVALGGDGLMLQTLHHFRDKGVPIYGMHRGSVGFLMNT
YREDDLRARLANATHVVIHPLMMEAVDAAGVRYSAPAINEVSLLRQTYQAAKLRISIDGKVRLEELICDGVLVATPAGST
AYNLSAHGPILPLGAALLALTPISPFRPRRWRGALVPDRATIQIEILEADKRPVSAVADHKEVRDIIEVTARLDKTSSMD
MLFDPDHGLDERILREQFIY

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=156, Percent_Identity=31.4102564102564, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325068, Length=194, Percent_Identity=33.5051546391753, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322509, Length=206, Percent_Identity=28.6407766990291, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320794, Length=189, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 28734; Mature: 28734

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSPRRFERIAFMASGTPEAEAARARLIQRYGDVSEDAADVIVALGGDGLMLQTLHHFR
CCCCHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHH
DKGVPIYGMHRGSVGFLMNTYREDDLRARLANATHVVIHPLMMEAVDAAGVRYSAPAINE
HCCCCEEEECCCCHHHHHHHCCHHHHHHHHCCCHHEEHHHHHHHHHHHCCCEECCCCCHH
VSLLRQTYQAAKLRISIDGKVRLEELICDGVLVATPAGSTAYNLSAHGPILPLGAALLAL
HHHHHHHHHHHEEEEEECCCEEHHHHHHCCEEEECCCCCCEEECCCCCCCHHHHHHHHHH
TPISPFRPRRWRGALVPDRATIQIEILEADKRPVSAVADHKEVRDIIEVTARLDKTSSMD
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
MLFDPDHGLDERILREQFIY
EEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNDSPRRFERIAFMASGTPEAEAARARLIQRYGDVSEDAADVIVALGGDGLMLQTLHHFR
CCCCHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHH
DKGVPIYGMHRGSVGFLMNTYREDDLRARLANATHVVIHPLMMEAVDAAGVRYSAPAINE
HCCCCEEEECCCCHHHHHHHCCHHHHHHHHCCCHHEEHHHHHHHHHHHCCCEECCCCCHH
VSLLRQTYQAAKLRISIDGKVRLEELICDGVLVATPAGSTAYNLSAHGPILPLGAALLAL
HHHHHHHHHHHEEEEEECCCEEHHHHHHCCEEEECCCCCCEEECCCCCCCHHHHHHHHHH
TPISPFRPRRWRGALVPDRATIQIEILEADKRPVSAVADHKEVRDIIEVTARLDKTSSMD
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
MLFDPDHGLDERILREQFIY
EEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA