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The map label for this gene is folE

Identifier: 158422566

GI number: 158422566

Start: 1052449

End: 1053105

Strand: Reverse

Name: folE

Synonym: AZC_0942

Alternate gene names: 158422566

Gene position: 1053105-1052449 (Counterclockwise)

Preceding gene: 158422568

Following gene: 158422565

Centisome position: 19.61

GC content: 66.21

Gene sequence:

>657_bases
ATGGACGCGGTGGTGAAACTGTTCAAGGAACAGGCCGAGACCGAGGCGCAGCGGCAGATGCCGGTGCATCGCCCGGTGGA
TGCGCCGCGCCCCTCGCGCGCCGAGGCGGAAGCCGCCGTCCGCACCCTGCTCGCTTACATCGGCGAGGATCCCCATCGCG
AAGGCCTTCAGGAGACGCCCGCCCGCGTCATCCGCTCCTATGACGAGATCTATGGCGGCTACGCCCTCTCTCCCGACGAC
GTGCTGGACCGCACCTTCGGCGAGATCGGCAGCTTCGATGATTTCGTGCTGCTGAAGGACATCCCCTTCACCTCCCATTG
CGAGCACCACATGGTGCCCTTCATGGGCAAGGCACATGTGGCCTATTTCCCGGTGGAGCGTGTGGTGGGCCTCTCCAAGA
TCGCCCGGCTGGTCGATCTCTATGCCCGGCGCCTCCAGACCCAGGAGCACCTGACCTCGCAGATCACCACCGCCCTCGAC
GAGGCGCTGAAGCCGCGCGGCGTCGCGGTGATGATCGAGGCCGAGCATATGTGCATGTCCATGCGCGGCATCCTGAAGCC
CGGTGTGACAACGCTGACCAGCCAGTTCACCGGCGCCTTCCGCGACGACCCGGCCGAGCAGGTGCGATTCATCACCATGC
TGCGCGGCCTGCGCTGA

Upstream 100 bases:

>100_bases
TCTGTAGAGGCCGCAGGCTTCGACGAGCGGCGGCCAGCCTGCCGCTGATCAAAGCCGGTGACACTCCGCCTCCCCGGGGC
GGTCGTACGGAGTTGGGTTG

Downstream 100 bases:

>100_bases
CCCCCCATCCTGACGAGCGTGCCATGACCGAACCGACGCCGGCCTTCGCCAGGGCCACAGACGCGCATGAGCTGGAAGAG
GGCACGGTGCTGGCGCCGCG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MDAVVKLFKEQAETEAQRQMPVHRPVDAPRPSRAEAEAAVRTLLAYIGEDPHREGLQETPARVIRSYDEIYGGYALSPDD
VLDRTFGEIGSFDDFVLLKDIPFTSHCEHHMVPFMGKAHVAYFPVERVVGLSKIARLVDLYARRLQTQEHLTSQITTALD
EALKPRGVAVMIEAEHMCMSMRGILKPGVTTLTSQFTGAFRDDPAEQVRFITMLRGLR

Sequences:

>Translated_218_residues
MDAVVKLFKEQAETEAQRQMPVHRPVDAPRPSRAEAEAAVRTLLAYIGEDPHREGLQETPARVIRSYDEIYGGYALSPDD
VLDRTFGEIGSFDDFVLLKDIPFTSHCEHHMVPFMGKAHVAYFPVERVVGLSKIARLVDLYARRLQTQEHLTSQITTALD
EALKPRGVAVMIEAEHMCMSMRGILKPGVTTLTSQFTGAFRDDPAEQVRFITMLRGLR
>Mature_218_residues
MDAVVKLFKEQAETEAQRQMPVHRPVDAPRPSRAEAEAAVRTLLAYIGEDPHREGLQETPARVIRSYDEIYGGYALSPDD
VLDRTFGEIGSFDDFVLLKDIPFTSHCEHHMVPFMGKAHVAYFPVERVVGLSKIARLVDLYARRLQTQEHLTSQITTALD
EALKPRGVAVMIEAEHMCMSMRGILKPGVTTLTSQFTGAFRDDPAEQVRFITMLRGLR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=179, Percent_Identity=48.0446927374302, Blast_Score=177, Evalue=9e-45,
Organism=Homo sapiens, GI4503949, Length=179, Percent_Identity=48.0446927374302, Blast_Score=177, Evalue=9e-45,
Organism=Homo sapiens, GI66932970, Length=148, Percent_Identity=45.945945945946, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI66932972, Length=138, Percent_Identity=48.5507246376812, Blast_Score=134, Evalue=4e-32,
Organism=Escherichia coli, GI1788476, Length=198, Percent_Identity=37.3737373737374, Blast_Score=110, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=46.1111111111111, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321706, Length=178, Percent_Identity=47.752808988764, Blast_Score=165, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24656782, Length=178, Percent_Identity=44.9438202247191, Blast_Score=153, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24656796, Length=179, Percent_Identity=44.6927374301676, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24656791, Length=179, Percent_Identity=44.6927374301676, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_AZOC5 (A8HUG2)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523858.1
- ProteinModelPortal:   A8HUG2
- SMR:   A8HUG2
- GeneID:   5690362
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0942
- HOGENOM:   HBG370191
- OMA:   FSSHCEH
- ProtClustDB:   PRK09347
- BioCyc:   ACAU438753:AZC_0942-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 24540; Mature: 24540

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVVKLFKEQAETEAQRQMPVHRPVDAPRPSRAEAEAAVRTLLAYIGEDPHREGLQETP
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ARVIRSYDEIYGGYALSPDDVLDRTFGEIGSFDDFVLLKDIPFTSHCEHHMVPFMGKAHV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHH
AYFPVERVVGLSKIARLVDLYARRLQTQEHLTSQITTALDEALKPRGVAVMIEAEHMCMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
MRGILKPGVTTLTSQFTGAFRDDPAEQVRFITMLRGLR
HHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAVVKLFKEQAETEAQRQMPVHRPVDAPRPSRAEAEAAVRTLLAYIGEDPHREGLQETP
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ARVIRSYDEIYGGYALSPDDVLDRTFGEIGSFDDFVLLKDIPFTSHCEHHMVPFMGKAHV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHH
AYFPVERVVGLSKIARLVDLYARRLQTQEHLTSQITTALDEALKPRGVAVMIEAEHMCMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
MRGILKPGVTTLTSQFTGAFRDDPAEQVRFITMLRGLR
HHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA