Click here to switch to the map view.

The map label for this gene is cysH [H]

Identifier: 158422547

GI number: 158422547

Start: 1035188

End: 1035910

Strand: Reverse

Name: cysH [H]

Synonym: AZC_0923

Alternate gene names: 158422547

Gene position: 1035910-1035188 (Counterclockwise)

Preceding gene: 158422548

Following gene: 158422546

Centisome position: 19.29

GC content: 68.05

Gene sequence:

>723_bases
GTGTCCGCGCTTGCGACACAGATCGACCTGGCCGACGAGGCGGCGCGCCTCGCGGCAACGCTCGAGGGGGCGTCGCCGCT
CGACGTGCTGGAGCGCGCCATCGGCGGCCACCGGGGGCGCATCGCCGCCGTCTCCTCCTTCGGGACGGAATCTGCGGTGC
TGCTGCACATGATCGCGCAGGTGGATCGCTCGGTACCGGTGCTGTTCCTCGACACCGGCCATCTCTTCACCGAAACGCTC
CACTATCGCGACACGCTCATTGCCCGGCTCGGCCTCACGGACGTGCGCACCTACCGGCCGGACGAACAGCTTCGGGCGGA
GCGGGACGCCGACAACGACCTCTGGGCGAGCGATACGGATGCCTGCTGCGCCCTGCGCAAGGTGGAGCCGCTCGGGCGCG
CACTCGCGCCCTTTGAGGCCTGGATCAATGGCCGCAAGCGCTATCAGGCGGTGACGCGAGCGAACATTCCGTTCGTTGAA
GCGGACGAGGGCCGCATCAAGTTCAATCCACTGGCGGCGCTCGGGCCAGACGATATCGAGGGCTGGATGACCGCCCACGA
CCTGCCGCGCCATCCCATGCAGAGGTTCGGCTTCGCCTCCATCGGCTGCATGCCCTGCACGTCGCGGGTCAAGCCGGGGG
AAGATCCGCGGGCCGGCCGCTGGCGCGGACAGGCCAAGACGGAATGCGGCATCCACGTGCCAAAAGACAGCGAAGTTTTG
TAA

Upstream 100 bases:

>100_bases
AGCTGAAGACCTATTCCGTCTTCTACCAGCCCACCGGCGACGGACGGCCGACCCCGCTGCGGGCCCGCCTCGCGGCGGCG
CGCTCGGTCTGAGGCGCGCC

Downstream 100 bases:

>100_bases
TTTTTATTTTCATCGATTTTATTCAGTAAATCGATTGACCTTACAGAATGGGCCTGTATCGTTCGACGTGACGAACGAAT
GCGCCTCAAGGCGCGCCCAC

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSALATQIDLADEAARLAATLEGASPLDVLERAIGGHRGRIAAVSSFGTESAVLLHMIAQVDRSVPVLFLDTGHLFTETL
HYRDTLIARLGLTDVRTYRPDEQLRAERDADNDLWASDTDACCALRKVEPLGRALAPFEAWINGRKRYQAVTRANIPFVE
ADEGRIKFNPLAALGPDDIEGWMTAHDLPRHPMQRFGFASIGCMPCTSRVKPGEDPRAGRWRGQAKTECGIHVPKDSEVL

Sequences:

>Translated_240_residues
MSALATQIDLADEAARLAATLEGASPLDVLERAIGGHRGRIAAVSSFGTESAVLLHMIAQVDRSVPVLFLDTGHLFTETL
HYRDTLIARLGLTDVRTYRPDEQLRAERDADNDLWASDTDACCALRKVEPLGRALAPFEAWINGRKRYQAVTRANIPFVE
ADEGRIKFNPLAALGPDDIEGWMTAHDLPRHPMQRFGFASIGCMPCTSRVKPGEDPRAGRWRGQAKTECGIHVPKDSEVL
>Mature_239_residues
SALATQIDLADEAARLAATLEGASPLDVLERAIGGHRGRIAAVSSFGTESAVLLHMIAQVDRSVPVLFLDTGHLFTETLH
YRDTLIARLGLTDVRTYRPDEQLRAERDADNDLWASDTDACCALRKVEPLGRALAPFEAWINGRKRYQAVTRANIPFVEA
DEGRIKFNPLAALGPDDIEGWMTAHDLPRHPMQRFGFASIGCMPCTSRVKPGEDPRAGRWRGQAKTECGIHVPKDSEVL

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=199, Percent_Identity=32.6633165829146, Blast_Score=108, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6325425, Length=209, Percent_Identity=32.5358851674641, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 26397; Mature: 26266

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALATQIDLADEAARLAATLEGASPLDVLERAIGGHRGRIAAVSSFGTESAVLLHMIAQ
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
VDRSVPVLFLDTGHLFTETLHYRDTLIARLGLTDVRTYRPDEQLRAERDADNDLWASDTD
HCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHCCCCCCCCCCCHH
ACCALRKVEPLGRALAPFEAWINGRKRYQAVTRANIPFVEADEGRIKFNPLAALGPDDIE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCHHH
GWMTAHDLPRHPMQRFGFASIGCMPCTSRVKPGEDPRAGRWRGQAKTECGIHVPKDSEVL
HHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCC
>Mature Secondary Structure 
SALATQIDLADEAARLAATLEGASPLDVLERAIGGHRGRIAAVSSFGTESAVLLHMIAQ
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
VDRSVPVLFLDTGHLFTETLHYRDTLIARLGLTDVRTYRPDEQLRAERDADNDLWASDTD
HCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHCCCCCCCCCCCHH
ACCALRKVEPLGRALAPFEAWINGRKRYQAVTRANIPFVEADEGRIKFNPLAALGPDDIE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCHHH
GWMTAHDLPRHPMQRFGFASIGCMPCTSRVKPGEDPRAGRWRGQAKTECGIHVPKDSEVL
HHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]