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The map label for this gene is cysT [H]

Identifier: 158422545

GI number: 158422545

Start: 1033069

End: 1033920

Strand: Reverse

Name: cysT [H]

Synonym: AZC_0921

Alternate gene names: 158422545

Gene position: 1033920-1033069 (Counterclockwise)

Preceding gene: 158422546

Following gene: 158422544

Centisome position: 19.25

GC content: 65.38

Gene sequence:

>852_bases
ATGGCGGCAGCCACACGAGCTTCCTTCTTCCGGCCGCGCAGCGTCATTCCCGGCTTCGGGCTGACGTTCGGGCTGACCGT
CACGTGGCTGAGCCTCATCGTGCTCATCCCGCTCGCAGCCCTCTTCCTGAAGACCACGGAACTGACCCTCGACCGCTTCA
TTGCCGTGGTGACGGGCCCGCGCACGCTCCACGCGCTCTGGGTGTCGTTCGGCATCTCATTCGCGGCGGCGGTGGTGAAT
GCCGTGTTCGGGACCATCGTGGTCTGGGCCTTGGTGCGCTACGATTTCCCCGGCCGCCGGCTGGTGGATGCCATCGTGGA
CGTGCCCTTCGCGCTGCCGACGGCAGTGGCCGGCATTTCGCTGACCGCGCTTTATGCGGAAAACGGCTGGATCGGCAGCC
TGCTGGCGCCGCTCGGCATCAAGGTCGCCTTCACCCCGCTCGGCATCCTGGTGGCCCTCGTCTTTGTGGGCCTGCCCTTT
GTGGTGCGTACCATCCAGCCGGTTCTGGCGGATCTCGACAAGCAATATGAAGAGGCCGCCGCCAGCCTTGGCGCCAGTCG
CTTCGAGACCATCTGGCGCGTGGTGCTGCCCACCGTCTGGCCCGCGCTGCTCACCGGCTTTTCCCTCGCCTTCGCGCGGG
GCATCGGAGAATACGGCTCGGTCATCTTCATCGCCGGCAACATGCCGAACGTTTCGGAGATTGCGCCTCTGCTCATCGTC
GTTCGGCTGGAGGAGTTCCGCTATGCGGACGCCACTGCCATCGCCGTCGTGATGCTGGTCGCGTCCTTCATCCTTCTCCT
CATCATCAACCGGCTCCAGCAATGGACCGAACGGCGGACCAGCGAGCGATGA

Upstream 100 bases:

>100_bases
CTCCAGCCGGTGCGGGCTGGATCAAGGCGCGGCGGCAGGTTCCGCCGCATCCTACTGATCCGGTCCGGCACGGCGGGCCT
CTCACGACGCGGATGTCACC

Downstream 100 bases:

>100_bases
GCGTGGCCGATCTCGCCCCTCCCCCTGTCTCGGTTCAGGCGCAGGCGATCCGTCGCGCGCCGCCCGTGCACACGGAGCCG
CCGCTGGTGCGCTTCGCCGT

Product: sulphate transporter permease 2

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAAATRASFFRPRSVIPGFGLTFGLTVTWLSLIVLIPLAALFLKTTELTLDRFIAVVTGPRTLHALWVSFGISFAAAVVN
AVFGTIVVWALVRYDFPGRRLVDAIVDVPFALPTAVAGISLTALYAENGWIGSLLAPLGIKVAFTPLGILVALVFVGLPF
VVRTIQPVLADLDKQYEEAAASLGASRFETIWRVVLPTVWPALLTGFSLAFARGIGEYGSVIFIAGNMPNVSEIAPLLIV
VRLEEFRYADATAIAVVMLVASFILLLIINRLQQWTERRTSER

Sequences:

>Translated_283_residues
MAAATRASFFRPRSVIPGFGLTFGLTVTWLSLIVLIPLAALFLKTTELTLDRFIAVVTGPRTLHALWVSFGISFAAAVVN
AVFGTIVVWALVRYDFPGRRLVDAIVDVPFALPTAVAGISLTALYAENGWIGSLLAPLGIKVAFTPLGILVALVFVGLPF
VVRTIQPVLADLDKQYEEAAASLGASRFETIWRVVLPTVWPALLTGFSLAFARGIGEYGSVIFIAGNMPNVSEIAPLLIV
VRLEEFRYADATAIAVVMLVASFILLLIINRLQQWTERRTSER
>Mature_282_residues
AAATRASFFRPRSVIPGFGLTFGLTVTWLSLIVLIPLAALFLKTTELTLDRFIAVVTGPRTLHALWVSFGISFAAAVVNA
VFGTIVVWALVRYDFPGRRLVDAIVDVPFALPTAVAGISLTALYAENGWIGSLLAPLGIKVAFTPLGILVALVFVGLPFV
VRTIQPVLADLDKQYEEAAASLGASRFETIWRVVLPTVWPALLTGFSLAFARGIGEYGSVIFIAGNMPNVSEIAPLLIVV
RLEEFRYADATAIAVVMLVASFILLLIINRLQQWTERRTSER

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=267, Percent_Identity=48.689138576779, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87082099, Length=284, Percent_Identity=32.0422535211268, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786980, Length=221, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787369, Length=175, Percent_Identity=27.4285714285714, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786254, Length=231, Percent_Identity=25.5411255411255, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787368, Length=199, Percent_Identity=31.1557788944724, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30703; Mature: 30572

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAATRASFFRPRSVIPGFGLTFGLTVTWLSLIVLIPLAALFLKTTELTLDRFIAVVTGP
CCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTLHALWVSFGISFAAAVVNAVFGTIVVWALVRYDFPGRRLVDAIVDVPFALPTAVAGIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHH
LTALYAENGWIGSLLAPLGIKVAFTPLGILVALVFVGLPFVVRTIQPVLADLDKQYEEAA
HEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLGASRFETIWRVVLPTVWPALLTGFSLAFARGIGEYGSVIFIAGNMPNVSEIAPLLIV
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
VRLEEFRYADATAIAVVMLVASFILLLIINRLQQWTERRTSER
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAATRASFFRPRSVIPGFGLTFGLTVTWLSLIVLIPLAALFLKTTELTLDRFIAVVTGP
CCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTLHALWVSFGISFAAAVVNAVFGTIVVWALVRYDFPGRRLVDAIVDVPFALPTAVAGIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHH
LTALYAENGWIGSLLAPLGIKVAFTPLGILVALVFVGLPFVVRTIQPVLADLDKQYEEAA
HEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLGASRFETIWRVVLPTVWPALLTGFSLAFARGIGEYGSVIFIAGNMPNVSEIAPLLIV
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
VRLEEFRYADATAIAVVMLVASFILLLIINRLQQWTERRTSER
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]