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The map label for this gene is nadE [H]

Identifier: 158422537

GI number: 158422537

Start: 1022010

End: 1023710

Strand: Reverse

Name: nadE [H]

Synonym: AZC_0913

Alternate gene names: 158422537

Gene position: 1023710-1022010 (Counterclockwise)

Preceding gene: 158422541

Following gene: 158422534

Centisome position: 19.06

GC content: 67.96

Gene sequence:

>1701_bases
ATGACCGCTTCTCTCTCCGCATCCGAAACCGCCGCCATCGTCCCTGCCGAAACGCTGCGCATCGCGGTGGCGCAGTTGAA
CCCGGTGGTGGGCGATGTTGCCGGCAATGCGGAGAAGGTCCGCATGGCGCGGGACGAGGCGGCGCTACAGGGCGCGGACC
TCGTGGTCTTCTCCGAACTGTTCCTCGCCGGCTATCCGCCGGAGGATCTGGTGCTGAAGCCCGCCTTCCAGCAGGCCTGC
CGCGCGGCGCTGGACGAGCTCGCGGCGGAGACCGTCGGCGGCCCGGCGCTGCTGGTTGGCGCGCCCTGGGTGGAGGACGG
GCACCTGCACAATGGCGTCTTCCTACTGGAAGGCGGACGCATCGCGGCCAAGCGCTTCAAGGTGGACCTGCCGAATTATG
GCGTCTTCGACGAGAAGCGCGTGTTCGTGCCCGGCCCCCTGCCCGGCCCGATCCCCTTCAAGGGCGTTCGCCTTGGCGTG
CCGGTGTGCGAGGACATCTGGGGGCCGGACGTGGTGGAGTGCCTGTCCGAGACGGGCGCCGAAATCCTCATCGTGCCCAA
CGGCTCGCCCTATCGCCGCTCGGTCTATGACGAGCGGGCCAATATCGCGGTGGCCCGCGTGGTGGAGAGCGGCCTGCCGC
TGCTCTATGTCAATCAGGTGGGCGGGCAGGACGAACTGGTGTTCGACGGCGCCTCCTTCGCCCTCAATGCCGACCGCACG
CAGGTGATGCAGTTCCCCGGCTTTGCCGAGCGGGTGCGCACCACGCAGTGGCGGCGCTATGAGAACGGCTGGAAGTGCGA
GGAAGCCCCCTTCGCCGACCGCCTCGAAGGCGACCGGGCGGACTATGCGGCCTGCGTCCTCGGCCTCAAGGACTATGTGA
ACAAGAACCGCTTCCCCGGCGTGGTGCTCGGCCTCTCCGGCGGCATCGACAGTGCCATCTGCGCCGCCATGGCGGTGGAT
GCACTGGGACCGGAGCGGGTCCACTGCATCATGCTGCCCTATCGCTTCACCTCGCAGGACAGCCTCTCGGATGCCGCCGA
CGTCGCCCGCCGCCTCGGCGTGCGCTACGACACGCTGCCCATCGCGCCCGCCGTGGAGGCGCTGGAGGGCGTGCTCGCCC
CCCTCTTCGCCGGGCGGGAACGGGACGTGACGGAGGAGAACCTCCAGTCGCGCGTCCGCGGTACGCTGCTCATGTCCGTC
TCCAACAAGTTCGGAGCCATGGTGGTGACCACCGGCAACAAGTCGGAGATGTCCACCGGCTATGCGACGCTCTATGGCGA
CATGAACGGCGGCTACAATCCCATCAAGGATCTCTACAAGACCGAGGTCTTCCGCCTCTCCCGCCTGCGCAACACCTGGA
AGCCCACGGGCGCGCTGGGACCGGACGGCATCGTCATTCCCGACGCCCTCATCGACAAGCCGCCGACGGCCGAATTGCGC
GAGAACCAGAAGGATCAGGATTCGCTGCCGCCCTATGAGGTGCTGGACGCCATCCTCGCCCGCCTTGTGGAAGCGGAGAC
CCCTGTGGCGCAGCTCATCGCGGACGGCTTCGACCCGGCGGTGGTGAAGCGCGTGGAGCGGATGCTGAACATCGCCGAAT
ACAAGCGCCGGCAGGCGGCGCCGGGCGTGAAGGTGACGACCCGCAACTTCGGCCGCGACCGGCGCTATCCCATCACCAAC
GGTTTCCGCGATCTCGGCTGA

Upstream 100 bases:

>100_bases
AGACACCCGCCGAGCCGGCGGAGCGCCCATGCGGCCCGCATGCATTGCCGCATGGGCGCGGCGCGGCTACATCTGGGGCG
CCATTTTCTGCCGGGGCGCC

Downstream 100 bases:

>100_bases
CGCCGGCGCGCTTCACGCGACGGACACGACATCGTCACTGCCGAGCGGCTCGGCGGCGTCACCGTCGCGGCCCTCCAATA
TGGCGCGGCGCTTGCCGAGT

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTASLSASETAAIVPAETLRIAVAQLNPVVGDVAGNAEKVRMARDEAALQGADLVVFSELFLAGYPPEDLVLKPAFQQAC
RAALDELAAETVGGPALLVGAPWVEDGHLHNGVFLLEGGRIAAKRFKVDLPNYGVFDEKRVFVPGPLPGPIPFKGVRLGV
PVCEDIWGPDVVECLSETGAEILIVPNGSPYRRSVYDERANIAVARVVESGLPLLYVNQVGGQDELVFDGASFALNADRT
QVMQFPGFAERVRTTQWRRYENGWKCEEAPFADRLEGDRADYAACVLGLKDYVNKNRFPGVVLGLSGGIDSAICAAMAVD
ALGPERVHCIMLPYRFTSQDSLSDAADVARRLGVRYDTLPIAPAVEALEGVLAPLFAGRERDVTEENLQSRVRGTLLMSV
SNKFGAMVVTTGNKSEMSTGYATLYGDMNGGYNPIKDLYKTEVFRLSRLRNTWKPTGALGPDGIVIPDALIDKPPTAELR
ENQKDQDSLPPYEVLDAILARLVEAETPVAQLIADGFDPAVVKRVERMLNIAEYKRRQAAPGVKVTTRNFGRDRRYPITN
GFRDLG

Sequences:

>Translated_566_residues
MTASLSASETAAIVPAETLRIAVAQLNPVVGDVAGNAEKVRMARDEAALQGADLVVFSELFLAGYPPEDLVLKPAFQQAC
RAALDELAAETVGGPALLVGAPWVEDGHLHNGVFLLEGGRIAAKRFKVDLPNYGVFDEKRVFVPGPLPGPIPFKGVRLGV
PVCEDIWGPDVVECLSETGAEILIVPNGSPYRRSVYDERANIAVARVVESGLPLLYVNQVGGQDELVFDGASFALNADRT
QVMQFPGFAERVRTTQWRRYENGWKCEEAPFADRLEGDRADYAACVLGLKDYVNKNRFPGVVLGLSGGIDSAICAAMAVD
ALGPERVHCIMLPYRFTSQDSLSDAADVARRLGVRYDTLPIAPAVEALEGVLAPLFAGRERDVTEENLQSRVRGTLLMSV
SNKFGAMVVTTGNKSEMSTGYATLYGDMNGGYNPIKDLYKTEVFRLSRLRNTWKPTGALGPDGIVIPDALIDKPPTAELR
ENQKDQDSLPPYEVLDAILARLVEAETPVAQLIADGFDPAVVKRVERMLNIAEYKRRQAAPGVKVTTRNFGRDRRYPITN
GFRDLG
>Mature_565_residues
TASLSASETAAIVPAETLRIAVAQLNPVVGDVAGNAEKVRMARDEAALQGADLVVFSELFLAGYPPEDLVLKPAFQQACR
AALDELAAETVGGPALLVGAPWVEDGHLHNGVFLLEGGRIAAKRFKVDLPNYGVFDEKRVFVPGPLPGPIPFKGVRLGVP
VCEDIWGPDVVECLSETGAEILIVPNGSPYRRSVYDERANIAVARVVESGLPLLYVNQVGGQDELVFDGASFALNADRTQ
VMQFPGFAERVRTTQWRRYENGWKCEEAPFADRLEGDRADYAACVLGLKDYVNKNRFPGVVLGLSGGIDSAICAAMAVDA
LGPERVHCIMLPYRFTSQDSLSDAADVARRLGVRYDTLPIAPAVEALEGVLAPLFAGRERDVTEENLQSRVRGTLLMSVS
NKFGAMVVTTGNKSEMSTGYATLYGDMNGGYNPIKDLYKTEVFRLSRLRNTWKPTGALGPDGIVIPDALIDKPPTAELRE
NQKDQDSLPPYEVLDAILARLVEAETPVAQLIADGFDPAVVKRVERMLNIAEYKRRQAAPGVKVTTRNFGRDRRYPITNG
FRDLG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61492; Mature: 61361

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLSASETAAIVPAETLRIAVAQLNPVVGDVAGNAEKVRMARDEAALQGADLVVFSEL
CCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHH
FLAGYPPEDLVLKPAFQQACRAALDELAAETVGGPALLVGAPWVEDGHLHNGVFLLEGGR
HHCCCCCHHCEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCE
IAAKRFKVDLPNYGVFDEKRVFVPGPLPGPIPFKGVRLGVPVCEDIWGPDVVECLSETGA
EEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCEEECCCHHHHCCCHHHHHHHHHCCC
EILIVPNGSPYRRSVYDERANIAVARVVESGLPLLYVNQVGGQDELVFDGASFALNADRT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEECCCHH
QVMQFPGFAERVRTTQWRRYENGWKCEEAPFADRLEGDRADYAACVLGLKDYVNKNRFPG
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCE
VVLGLSGGIDSAICAAMAVDALGPERVHCIMLPYRFTSQDSLSDAADVARRLGVRYDTLP
EEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
IAPAVEALEGVLAPLFAGRERDVTEENLQSRVRGTLLMSVSNKFGAMVVTTGNKSEMSTG
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCC
YATLYGDMNGGYNPIKDLYKTEVFRLSRLRNTWKPTGALGPDGIVIPDALIDKPPTAELR
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHCCCCCCHHHH
ENQKDQDSLPPYEVLDAILARLVEAETPVAQLIADGFDPAVVKRVERMLNIAEYKRRQAA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
PGVKVTTRNFGRDRRYPITNGFRDLG
CCCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TASLSASETAAIVPAETLRIAVAQLNPVVGDVAGNAEKVRMARDEAALQGADLVVFSEL
CCCCCCCCCEEECCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHH
FLAGYPPEDLVLKPAFQQACRAALDELAAETVGGPALLVGAPWVEDGHLHNGVFLLEGGR
HHCCCCCHHCEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCE
IAAKRFKVDLPNYGVFDEKRVFVPGPLPGPIPFKGVRLGVPVCEDIWGPDVVECLSETGA
EEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCEEECCCHHHHCCCHHHHHHHHHCCC
EILIVPNGSPYRRSVYDERANIAVARVVESGLPLLYVNQVGGQDELVFDGASFALNADRT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEECCCHH
QVMQFPGFAERVRTTQWRRYENGWKCEEAPFADRLEGDRADYAACVLGLKDYVNKNRFPG
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCE
VVLGLSGGIDSAICAAMAVDALGPERVHCIMLPYRFTSQDSLSDAADVARRLGVRYDTLP
EEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
IAPAVEALEGVLAPLFAGRERDVTEENLQSRVRGTLLMSVSNKFGAMVVTTGNKSEMSTG
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCC
YATLYGDMNGGYNPIKDLYKTEVFRLSRLRNTWKPTGALGPDGIVIPDALIDKPPTAELR
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHCCCCCCHHHH
ENQKDQDSLPPYEVLDAILARLVEAETPVAQLIADGFDPAVVKRVERMLNIAEYKRRQAA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
PGVKVTTRNFGRDRRYPITNGFRDLG
CCCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]