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The map label for this gene is ispE

Identifier: 158422534

GI number: 158422534

Start: 1018187

End: 1019056

Strand: Reverse

Name: ispE

Synonym: AZC_0910

Alternate gene names: 158422534

Gene position: 1019056-1018187 (Counterclockwise)

Preceding gene: 158422537

Following gene: 158422533

Centisome position: 18.98

GC content: 71.38

Gene sequence:

>870_bases
ATGCAAAGGCTTGTGGCGCGCGCGCCGGCAAAGGTCAATCTGACATTGCGCATCCTCCGGCGCCGTCCGGACGGGTTTCA
CGATCTCGCGAGTATCGTTGCATTTGCGGGCGCTTGCGATGTGGTGACGCTGGAGTCGGACGCCCCCCTCGGCCTTGCCG
TTGCGGGACCGTCCGCCGCTGCCGCAGGGCCGGATGCGGACAATCTCATCCTGCGCGCCGCCGATGCTTTCGCCCGCCAT
GTGCCCGGTGCCCGCCTCGGCCGTTTCACGCTCACCAAGCGCCTGCCGGTGGCGGCAGGAATCGGGGGAGGCTCCTCCGA
CGCGGCCGCCGCCTTGCGCCTGCTGGCGCAGATCAACGACATCGCGCTCGATGATCCGCGCCTCCACGCCGCAGCCAGGG
AGGTGGGCGCCGACGTGCCCGTCTGCCTCGATCCGCAGGCCCGGATCATGGAAGGCATCGGCGAGCGGCTGTCCGAGCCG
CTGGGTCTCGCACCGCTGTTCGCCGTGCTGGTGAACTGCCGCGTGCCGGTGCCGACGGCCGATGTCTTCCGCACGCTGGG
CCTCCGGGCCGGCGAGGATCTGGCGGGTCTGCCGCACGAGGCGCCCATGAGCACCGGCCGCGCGGCTGTGATGCGGCACC
TCGTCCTGCACGGCAACGATCTGGAACCGCCCGCCGAGACGGTAGCCCCCGAGATCACCCGCGTGAAGGCGATGCTGGCC
CAAGAACCGGAGGTCCGGCTGGTGCGGATGTCCGGTTCCGGTGCGACGGTTTTTGCGCTGACCGACGACTGCCGGGCCGC
CGCGCAGGTGGCACGGCGGGTCGCTGCCGCGGAACCCGGCTGGTGGGTCCGCCCCACCGTGCTGCGCTAG

Upstream 100 bases:

>100_bases
GGAGGATGGCCGCTTTCCCGACGCCTCGCAAGCAAGGCCACGCCGAGGGACGGGGACGGCGTGCTTGCCGCATGCCGCGG
ACCGGGCCATAAGCGAACCC

Downstream 100 bases:

>100_bases
GGGTGCCCATGAGCCTGCCCCTCTTTGCGCTGGCCGCGCTCGCCGAGATCGCCGGCTGCTTTGCCTTCTGGCATGTGGTG
CGCGCGGGCGGCAGCCCGCT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MQRLVARAPAKVNLTLRILRRRPDGFHDLASIVAFAGACDVVTLESDAPLGLAVAGPSAAAAGPDADNLILRAADAFARH
VPGARLGRFTLTKRLPVAAGIGGGSSDAAAALRLLAQINDIALDDPRLHAAAREVGADVPVCLDPQARIMEGIGERLSEP
LGLAPLFAVLVNCRVPVPTADVFRTLGLRAGEDLAGLPHEAPMSTGRAAVMRHLVLHGNDLEPPAETVAPEITRVKAMLA
QEPEVRLVRMSGSGATVFALTDDCRAAAQVARRVAAAEPGWWVRPTVLR

Sequences:

>Translated_289_residues
MQRLVARAPAKVNLTLRILRRRPDGFHDLASIVAFAGACDVVTLESDAPLGLAVAGPSAAAAGPDADNLILRAADAFARH
VPGARLGRFTLTKRLPVAAGIGGGSSDAAAALRLLAQINDIALDDPRLHAAAREVGADVPVCLDPQARIMEGIGERLSEP
LGLAPLFAVLVNCRVPVPTADVFRTLGLRAGEDLAGLPHEAPMSTGRAAVMRHLVLHGNDLEPPAETVAPEITRVKAMLA
QEPEVRLVRMSGSGATVFALTDDCRAAAQVARRVAAAEPGWWVRPTVLR
>Mature_289_residues
MQRLVARAPAKVNLTLRILRRRPDGFHDLASIVAFAGACDVVTLESDAPLGLAVAGPSAAAAGPDADNLILRAADAFARH
VPGARLGRFTLTKRLPVAAGIGGGSSDAAAALRLLAQINDIALDDPRLHAAAREVGADVPVCLDPQARIMEGIGERLSEP
LGLAPLFAVLVNCRVPVPTADVFRTLGLRAGEDLAGLPHEAPMSTGRAAVMRHLVLHGNDLEPPAETVAPEITRVKAMLA
QEPEVRLVRMSGSGATVFALTDDCRAAAQVARRVAAAEPGWWVRPTVLR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=281, Percent_Identity=29.1814946619217, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_AZOC5 (A8HU60)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523826.1
- ProteinModelPortal:   A8HU60
- SMR:   A8HU60
- GeneID:   5692195
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0910
- HOGENOM:   HBG734593
- OMA:   DISERRP
- ProtClustDB:   PRK14608
- BioCyc:   ACAU438753:AZC_0910-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30258; Mature: 30258

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLVARAPAKVNLTLRILRRRPDGFHDLASIVAFAGACDVVTLESDAPLGLAVAGPSAA
CCCCHHCCCCCEEHHHEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCC
AAGPDADNLILRAADAFARHVPGARLGRFTLTKRLPVAAGIGGGSSDAAAALRLLAQIND
CCCCCCCHHHHHHHHHHHHHCCCCHHCCEEHHHCCCEEECCCCCCHHHHHHHHHHHHHHH
IALDDPRLHAAAREVGADVPVCLDPQARIMEGIGERLSEPLGLAPLFAVLVNCRVPVPTA
HCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHH
DVFRTLGLRAGEDLAGLPHEAPMSTGRAAVMRHLVLHGNDLEPPAETVAPEITRVKAMLA
HHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QEPEVRLVRMSGSGATVFALTDDCRAAAQVARRVAAAEPGWWVRPTVLR
CCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQRLVARAPAKVNLTLRILRRRPDGFHDLASIVAFAGACDVVTLESDAPLGLAVAGPSAA
CCCCHHCCCCCEEHHHEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCC
AAGPDADNLILRAADAFARHVPGARLGRFTLTKRLPVAAGIGGGSSDAAAALRLLAQIND
CCCCCCCHHHHHHHHHHHHHCCCCHHCCEEHHHCCCEEECCCCCCHHHHHHHHHHHHHHH
IALDDPRLHAAAREVGADVPVCLDPQARIMEGIGERLSEPLGLAPLFAVLVNCRVPVPTA
HCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHH
DVFRTLGLRAGEDLAGLPHEAPMSTGRAAVMRHLVLHGNDLEPPAETVAPEITRVKAMLA
HHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QEPEVRLVRMSGSGATVFALTDDCRAAAQVARRVAAAEPGWWVRPTVLR
CCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA