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The map label for this gene is dnaB [H]

Identifier: 158422458

GI number: 158422458

Start: 939304

End: 940725

Strand: Direct

Name: dnaB [H]

Synonym: AZC_0834

Alternate gene names: 158422458

Gene position: 939304-940725 (Clockwise)

Preceding gene: 158422457

Following gene: 158422459

Centisome position: 17.49

GC content: 66.46

Gene sequence:

>1422_bases
ATGAGCGACACGCCGTCATCGCCATCGGCAGACCTGCTCCTCGAACAGGAACTGCTGGGCTGTCTCATCTGCGCGCCCGA
GGCTCTCGCAGCCGTGGCAAGCTCCCTCGAGCCCGAGCATTTCGCCCAGCCCCTCCATGGGCGCCTGTTCGAAGCCTTGA
AGGCGCGGCATGCGAGCGGGGCTCCCAGCAGCCCTGTGACATTGGCTGCGGCCTTGAAGATGGACTGGAGTGCACCGGTC
GCGGACGACATGACGGTCGGCCAGTACGTCGCGCGCCTGGTGGCGGACGCCGGGCCTCCGATCATGGCCAAGAGCTATTC
CGCCGACCTGCGGGACATGTGGGCAGCCCGCGCCATCGGCGATGCGGTCAGCAGGACGGCGTCGTCGAACGGCATGCCCA
ACGAGAAGCTGGAGGCGCTGCTCGACGAGATCGACACCGTGCGCGCCAGCATGGCGGATGCTCAGGTCACTCGCGAGACG
GCGGGAGAGAGCGCCCAGCGCCTCCTCGAGCAGATCAACGGCATTCGGTGCGGAGAGGCGCAGCCCTCCGGCGCCACCAC
GGGTTTCACGGATCTCGACCGCATCATGCTGGGATATCGCCCTGGCGAACTGATCGTGCTCGGAGCCCGCCCGGGCGTCG
GGAAGACCACGTTCGGAACGTCATCGCTCCTGCAAAGCGCGAAGCAGGGGAATGGCGTGATGCTGTTCTCCCTTGAGCTG
TCTCGCGAGGCGGTGGCTGCGCGGCTCCAGGCGGATCTGGTCTTTGACGCCCGGTTTCCTCTCACCCATTCGCAGATCCG
CGCCGGCAACGGCCTGTCGGACCACGACTTCGAACGGATCATGGGCGCGACCGCGCGTATGAACGCCCTGCCGTTTGAGA
TCGAATATGCGCCGCGCCTCGCGGTAGCGGACCTCGGAGCGCGCGTATCGGCGGCCCGGAAGCGTCTCGCGCGCGCAGGT
GTGACCCTCCGCGTTGTTGCGGTCGACTATCTCAAGTTCCTCAAGGCGACCGACCGCTACAAGGGCCAGAGGGCCTATGA
GGTTGGTGAGATCACCGCCGGCCTGCTGGCCATCGCCAAGGACGAGGGCGTCTGCATCCTCCTGCTGGCCCAGCTCAACC
GCGGGATCGAGAGCGAGAAGGACAAGCGGCCCGACCTCCACCACCTGCGGGAGAGCGGAGACATCGAGAGCGACGCCAAC
GTCGTGATGTTCCTCTACCGGGAGGCCTACTACCTCGAGAAGTCGGAGGCCTACCGCTCCGGCGACCTCCAGGCCCTCGC
CGAGCACGAGCATGTGAGGAACAAGCTCGAAGTGGTCGTCGCCAAGAACCGCAATGGGCCCACGGGAACCGTGGACCTGT
TCTGCGACATCGGCGCGTCCGCAATCCGCAATCTCGACTGGCGCTATGGAGGGCACACCTGA

Upstream 100 bases:

>100_bases
AGGACGGCATTGCCGCAGGCAAGGCCTGGCGGAGCTGGCTGGACCTCTTCATGTCTGCGGCGCAGCGGGACGACCTGACG
AAGCCCGTGGTCCTCCGCCA

Downstream 100 bases:

>100_bases
TGGTCGATTTTTATCGACACGACATCCCGAGCTGGATGGACGGGACCGAGAGCCTGAGCGCCAACGAATATCGCGCGTAC
CATGTGATCTGCCAGCTTCT

Product: DnaB helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSDTPSSPSADLLLEQELLGCLICAPEALAAVASSLEPEHFAQPLHGRLFEALKARHASGAPSSPVTLAAALKMDWSAPV
ADDMTVGQYVARLVADAGPPIMAKSYSADLRDMWAARAIGDAVSRTASSNGMPNEKLEALLDEIDTVRASMADAQVTRET
AGESAQRLLEQINGIRCGEAQPSGATTGFTDLDRIMLGYRPGELIVLGARPGVGKTTFGTSSLLQSAKQGNGVMLFSLEL
SREAVAARLQADLVFDARFPLTHSQIRAGNGLSDHDFERIMGATARMNALPFEIEYAPRLAVADLGARVSAARKRLARAG
VTLRVVAVDYLKFLKATDRYKGQRAYEVGEITAGLLAIAKDEGVCILLLAQLNRGIESEKDKRPDLHHLRESGDIESDAN
VVMFLYREAYYLEKSEAYRSGDLQALAEHEHVRNKLEVVVAKNRNGPTGTVDLFCDIGASAIRNLDWRYGGHT

Sequences:

>Translated_473_residues
MSDTPSSPSADLLLEQELLGCLICAPEALAAVASSLEPEHFAQPLHGRLFEALKARHASGAPSSPVTLAAALKMDWSAPV
ADDMTVGQYVARLVADAGPPIMAKSYSADLRDMWAARAIGDAVSRTASSNGMPNEKLEALLDEIDTVRASMADAQVTRET
AGESAQRLLEQINGIRCGEAQPSGATTGFTDLDRIMLGYRPGELIVLGARPGVGKTTFGTSSLLQSAKQGNGVMLFSLEL
SREAVAARLQADLVFDARFPLTHSQIRAGNGLSDHDFERIMGATARMNALPFEIEYAPRLAVADLGARVSAARKRLARAG
VTLRVVAVDYLKFLKATDRYKGQRAYEVGEITAGLLAIAKDEGVCILLLAQLNRGIESEKDKRPDLHHLRESGDIESDAN
VVMFLYREAYYLEKSEAYRSGDLQALAEHEHVRNKLEVVVAKNRNGPTGTVDLFCDIGASAIRNLDWRYGGHT
>Mature_472_residues
SDTPSSPSADLLLEQELLGCLICAPEALAAVASSLEPEHFAQPLHGRLFEALKARHASGAPSSPVTLAAALKMDWSAPVA
DDMTVGQYVARLVADAGPPIMAKSYSADLRDMWAARAIGDAVSRTASSNGMPNEKLEALLDEIDTVRASMADAQVTRETA
GESAQRLLEQINGIRCGEAQPSGATTGFTDLDRIMLGYRPGELIVLGARPGVGKTTFGTSSLLQSAKQGNGVMLFSLELS
REAVAARLQADLVFDARFPLTHSQIRAGNGLSDHDFERIMGATARMNALPFEIEYAPRLAVADLGARVSAARKRLARAGV
TLRVVAVDYLKFLKATDRYKGQRAYEVGEITAGLLAIAKDEGVCILLLAQLNRGIESEKDKRPDLHHLRESGDIESDANV
VMFLYREAYYLEKSEAYRSGDLQALAEHEHVRNKLEVVVAKNRNGPTGTVDLFCDIGASAIRNLDWRYGGHT

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=454, Percent_Identity=29.0748898678414, Blast_Score=158, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51018; Mature: 50887

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPSSPSADLLLEQELLGCLICAPEALAAVASSLEPEHFAQPLHGRLFEALKARHASG
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHCCCC
APSSPVTLAAALKMDWSAPVADDMTVGQYVARLVADAGPPIMAKSYSADLRDMWAARAIG
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHH
DAVSRTASSNGMPNEKLEALLDEIDTVRASMADAQVTRETAGESAQRLLEQINGIRCGEA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCC
QPSGATTGFTDLDRIMLGYRPGELIVLGARPGVGKTTFGTSSLLQSAKQGNGVMLFSLEL
CCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEH
SREAVAARLQADLVFDARFPLTHSQIRAGNGLSDHDFERIMGATARMNALPFEIEYAPRL
HHHHHHHHHHHHEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCH
AVADLGARVSAARKRLARAGVTLRVVAVDYLKFLKATDRYKGQRAYEVGEITAGLLAIAK
HHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEC
DEGVCILLLAQLNRGIESEKDKRPDLHHLRESGDIESDANVVMFLYREAYYLEKSEAYRS
CCCEEEEEEHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCC
GDLQALAEHEHVRNKLEVVVAKNRNGPTGTVDLFCDIGASAIRNLDWRYGGHT
CCHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDTPSSPSADLLLEQELLGCLICAPEALAAVASSLEPEHFAQPLHGRLFEALKARHASG
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHCCCC
APSSPVTLAAALKMDWSAPVADDMTVGQYVARLVADAGPPIMAKSYSADLRDMWAARAIG
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHH
DAVSRTASSNGMPNEKLEALLDEIDTVRASMADAQVTRETAGESAQRLLEQINGIRCGEA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCC
QPSGATTGFTDLDRIMLGYRPGELIVLGARPGVGKTTFGTSSLLQSAKQGNGVMLFSLEL
CCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEH
SREAVAARLQADLVFDARFPLTHSQIRAGNGLSDHDFERIMGATARMNALPFEIEYAPRL
HHHHHHHHHHHHEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCH
AVADLGARVSAARKRLARAGVTLRVVAVDYLKFLKATDRYKGQRAYEVGEITAGLLAIAK
HHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEC
DEGVCILLLAQLNRGIESEKDKRPDLHHLRESGDIESDANVVMFLYREAYYLEKSEAYRS
CCCEEEEEEHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCC
GDLQALAEHEHVRNKLEVVVAKNRNGPTGTVDLFCDIGASAIRNLDWRYGGHT
CCHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA