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The map label for this gene is rlmN

Identifier: 158422376

GI number: 158422376

Start: 846460

End: 847680

Strand: Reverse

Name: rlmN

Synonym: AZC_0752

Alternate gene names: 158422376

Gene position: 847680-846460 (Counterclockwise)

Preceding gene: 158422377

Following gene: 158422375

Centisome position: 15.79

GC content: 68.22

Gene sequence:

>1221_bases
ATGTATGCGCCCATGATGCCTTCGCCGACTGCCGTGGCCGCCCCCGTGGCGGCGCTTTCCATTCTGCCCTCCCTCGCCGG
TCTCGACCGGGAGAAGCTGGGGCTTGCGCTCGCCGCCATCGGCGTGCCGGAGCGCGAGCGGAAGATGCGCGTCAACCAGC
TATGGCACTGGATCTACCTGCGCGGCGCCACCGACTTTGCGGAAATGACCAACGTCTCCAAGACGTTGCGGACGCAGCTG
GCGGAGCATTATTCCCTCGCCCGGCCCGAGATCGTGGTGGAGCAGGTGTCACAGGACGGCACCCGCAAGTGGCTGCTGCG
CCTGCCGGCGGAAACGCCCGGCGAGCGCCCCCACGAAGTGGAGGCGGTCTATATCCCCGAGCGGGATCGCGGCACGCTGT
GCGTCTCCAGCCAGGTGGGCTGCACGCTGAACTGCGCCTTCTGCCACACCGGCACGCAGCGGCTCGTGCGCAACCTCACC
GCCGCCGAAATCGTGGCGCAGGTGCTCGTCGCCCGCGACCGGCTGGGCGACTATCCCGGCCGTGAGCGCGCGGTAGGCCC
CGGCCTGCCCACGGAAGGCGACCGCCTCGTCACCAACATCGTCTTCATGGGCATGGGCGAGCCGCTCTATGCCTATGACT
CGGTCGCCGAGGCCATCGAGATCCTGGCCGATGGCGACGGCCTCGGCCTCGGCAAACGCCGCATCACCGTCTCCACCTCG
GGCGTGGTGCCTGAGATCGAGAAGCTCGGCCGCGAGGTCGGTCCCATGCTCGCCATCTCGCTGCACGCGGTGCGCGACGA
CCTGCGCGACGTGCTGGTGCCCATCAACAAGAAGTATCCCATCGCCGAGCTGATGGAGGCCTGCCGCACCTATCCGGCCG
CCTCCAACGCCAAGCGCATCACCTTTGAATATGTGATGCTGAAGGGCGTGAACGACAGCCCGGCGGACGCCCGCGCCCTG
GTGAAGCTGCTGGAAGGCGTGCCGGCCAAGATCAACCTCATTCCCTTCAACCCCTGGCCCGGCACCAAATATGAGTGCTC
GGACTGGGAGACGATCGAGAAGTTCTCGGACATCGTCTTCCGCGCCGGCTATGCGAGCCCGGTGCGCACCCCGCGCGGGC
GCGACATCCTCGCCGCCTGCGGCCAGTTGAAGAGCGAGACCGAGAAACTCTCCGCCCGCGAGCGCCTCGCACTCCGGGCC
ATGGCGGCCGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GAATGCCGCTGACCGCCTGAAACGGCCCGACCCGCCTGCCGGCCCCGCCGGCAGGCCCCTCATGCTTGAAGCGCGGTAGC
CGGACGGGCGGGAATTCATT

Downstream 100 bases:

>100_bases
CACCCGGCCAAGGGAGTTGGCATGATCGACCATCTGGGCCTTGCCGTCAGCGATCTTGAGCGCTCCCGCTCCTTTTATCT
CGCAGCGCTCGGCCCGATCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MYAPMMPSPTAVAAPVAALSILPSLAGLDREKLGLALAAIGVPERERKMRVNQLWHWIYLRGATDFAEMTNVSKTLRTQL
AEHYSLARPEIVVEQVSQDGTRKWLLRLPAETPGERPHEVEAVYIPERDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLT
AAEIVAQVLVARDRLGDYPGRERAVGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVAEAIEILADGDGLGLGKRRITVSTS
GVVPEIEKLGREVGPMLAISLHAVRDDLRDVLVPINKKYPIAELMEACRTYPAASNAKRITFEYVMLKGVNDSPADARAL
VKLLEGVPAKINLIPFNPWPGTKYECSDWETIEKFSDIVFRAGYASPVRTPRGRDILAACGQLKSETEKLSARERLALRA
MAAAAE

Sequences:

>Translated_406_residues
MYAPMMPSPTAVAAPVAALSILPSLAGLDREKLGLALAAIGVPERERKMRVNQLWHWIYLRGATDFAEMTNVSKTLRTQL
AEHYSLARPEIVVEQVSQDGTRKWLLRLPAETPGERPHEVEAVYIPERDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLT
AAEIVAQVLVARDRLGDYPGRERAVGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVAEAIEILADGDGLGLGKRRITVSTS
GVVPEIEKLGREVGPMLAISLHAVRDDLRDVLVPINKKYPIAELMEACRTYPAASNAKRITFEYVMLKGVNDSPADARAL
VKLLEGVPAKINLIPFNPWPGTKYECSDWETIEKFSDIVFRAGYASPVRTPRGRDILAACGQLKSETEKLSARERLALRA
MAAAAE
>Mature_406_residues
MYAPMMPSPTAVAAPVAALSILPSLAGLDREKLGLALAAIGVPERERKMRVNQLWHWIYLRGATDFAEMTNVSKTLRTQL
AEHYSLARPEIVVEQVSQDGTRKWLLRLPAETPGERPHEVEAVYIPERDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLT
AAEIVAQVLVARDRLGDYPGRERAVGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVAEAIEILADGDGLGLGKRRITVSTS
GVVPEIEKLGREVGPMLAISLHAVRDDLRDVLVPINKKYPIAELMEACRTYPAASNAKRITFEYVMLKGVNDSPADARAL
VKLLEGVPAKINLIPFNPWPGTKYECSDWETIEKFSDIVFRAGYASPVRTPRGRDILAACGQLKSETEKLSARERLALRA
MAAAAE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=387, Percent_Identity=44.1860465116279, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_AZOC5 (A8IQ73)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523668.1
- ProteinModelPortal:   A8IQ73
- GeneID:   5688695
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0752
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK979227
- BioCyc:   ACAU438753:AZC_0752-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR009056
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 44487; Mature: 44487

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS51007 CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAPMMPSPTAVAAPVAALSILPSLAGLDREKLGLALAAIGVPERERKMRVNQLWHWIYL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCEEHEECCCCHHHHHHHHHHHEEEEEE
RGATDFAEMTNVSKTLRTQLAEHYSLARPEIVVEQVSQDGTRKWLLRLPAETPGERPHEV
ECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCE
EAVYIPERDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLTAAEIVAQVLVARDRLGDYPG
EEEECCCCCCCCEEEECCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RERAVGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVAEAIEILADGDGLGLGKRRITVSTS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
GVVPEIEKLGREVGPMLAISLHAVRDDLRDVLVPINKKYPIAELMEACRTYPAASNAKRI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEE
TFEYVMLKGVNDSPADARALVKLLEGVPAKINLIPFNPWPGTKYECSDWETIEKFSDIVF
EEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
RAGYASPVRTPRGRDILAACGQLKSETEKLSARERLALRAMAAAAE
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYAPMMPSPTAVAAPVAALSILPSLAGLDREKLGLALAAIGVPERERKMRVNQLWHWIYL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCEEHEECCCCHHHHHHHHHHHEEEEEE
RGATDFAEMTNVSKTLRTQLAEHYSLARPEIVVEQVSQDGTRKWLLRLPAETPGERPHEV
ECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCE
EAVYIPERDRGTLCVSSQVGCTLNCAFCHTGTQRLVRNLTAAEIVAQVLVARDRLGDYPG
EEEECCCCCCCCEEEECCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RERAVGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVAEAIEILADGDGLGLGKRRITVSTS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
GVVPEIEKLGREVGPMLAISLHAVRDDLRDVLVPINKKYPIAELMEACRTYPAASNAKRI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEE
TFEYVMLKGVNDSPADARALVKLLEGVPAKINLIPFNPWPGTKYECSDWETIEKFSDIVF
EEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
RAGYASPVRTPRGRDILAACGQLKSETEKLSARERLALRAMAAAAE
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA