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The map label for this gene is dut

Identifier: 158422331

GI number: 158422331

Start: 786507

End: 786980

Strand: Direct

Name: dut

Synonym: AZC_0707

Alternate gene names: 158422331

Gene position: 786507-786980 (Clockwise)

Preceding gene: 158422330

Following gene: 158422332

Centisome position: 14.65

GC content: 66.88

Gene sequence:

>474_bases
ATGAGCGCGACGCGCCTTGACGTGCCCGTGGTCCGCCTGCCGCATGGCATGGACCTGCCTTTGCCCGAATATGCCACGGC
CGCCTCGGCGGGCATGGACCTTGTGGCCGCCGTGCCGGCCGAGGTGCCGCTCGTGATTGCCCCCGGGGCCTGGGCGTTGG
TCCCCACTGGGCTGGCCCTCGAATTGCCCGAGGGCATGGAGGCGCAGGTGCGCCCCCGCTCCGGCCTCGCGCTCAAGTTC
GGCGTCACCGTGCTCAACGCGCCGGGCACCATCGATGCCGACTATCGCGGCGAGGTCGGCGTCATCCTCATCAACCACGG
ACGCGAACCCTTCACGGTTTCCCGTGGAATGCGCATCGCACAGATGGTGATTGCGGGAGTTCAACAAATAACGCTGGTGG
AAACTGGAAATTTGGGCGAAACTGCACGCGGCGTCGGTGGTTTCGGCTCGACAGGGCTCGACCGGACGCCCTAA

Upstream 100 bases:

>100_bases
CCTGACCTTCGAAGACTTAACTCCAAGCCACAACTCCGGCCCCGCCGGACCGTGCCCCATCCCTCCGGCGGCCGCGCCGC
CTTGCATCCGAGGATGACAG

Downstream 100 bases:

>100_bases
CCGGGATAGCCACAGCCCCATGCCGCGTTTGTCCGACAAGAGTCTTCTCGCCATCGCCGCCGTCGTCGATGTCGCCGTTC
ACGCCCGTGGAACGCCGGTG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MSATRLDVPVVRLPHGMDLPLPEYATAASAGMDLVAAVPAEVPLVIAPGAWALVPTGLALELPEGMEAQVRPRSGLALKF
GVTVLNAPGTIDADYRGEVGVILINHGREPFTVSRGMRIAQMVIAGVQQITLVETGNLGETARGVGGFGSTGLDRTP

Sequences:

>Translated_157_residues
MSATRLDVPVVRLPHGMDLPLPEYATAASAGMDLVAAVPAEVPLVIAPGAWALVPTGLALELPEGMEAQVRPRSGLALKF
GVTVLNAPGTIDADYRGEVGVILINHGREPFTVSRGMRIAQMVIAGVQQITLVETGNLGETARGVGGFGSTGLDRTP
>Mature_156_residues
SATRLDVPVVRLPHGMDLPLPEYATAASAGMDLVAAVPAEVPLVIAPGAWALVPTGLALELPEGMEAQVRPRSGLALKFG
VTVLNAPGTIDADYRGEVGVILINHGREPFTVSRGMRIAQMVIAGVQQITLVETGNLGETARGVGGFGSTGLDRTP

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=133, Percent_Identity=42.8571428571429, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI4503423, Length=133, Percent_Identity=42.8571428571429, Blast_Score=98, Evalue=3e-21,
Organism=Homo sapiens, GI70906441, Length=133, Percent_Identity=42.8571428571429, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=41.6107382550336, Blast_Score=107, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71988561, Length=151, Percent_Identity=41.7218543046358, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319729, Length=155, Percent_Identity=35.4838709677419, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24583610, Length=154, Percent_Identity=40.2597402597403, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921126, Length=154, Percent_Identity=40.2597402597403, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_AZOC5 (A8IPW5)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523623.1
- ProteinModelPortal:   A8IPW5
- SMR:   A8IPW5
- GeneID:   5688970
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0707
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BioCyc:   ACAU438753:AZC_0707-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16233; Mature: 16102

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: BINDING 86-86

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATRLDVPVVRLPHGMDLPLPEYATAASAGMDLVAAVPAEVPLVIAPGAWALVPTGLAL
CCCCCCCCCEEECCCCCCCCCCCHHHHHHCCCEEEEECCCCCCEEECCCCCEEECCCEEE
ELPEGMEAQVRPRSGLALKFGVTVLNAPGTIDADYRGEVGVILINHGREPFTVSRGMRIA
ECCCCCCEEECCCCCEEEEECEEEEECCCCCCCCCCCCEEEEEEECCCCCEEHHCCCHHH
QMVIAGVQQITLVETGNLGETARGVGGFGSTGLDRTP
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SATRLDVPVVRLPHGMDLPLPEYATAASAGMDLVAAVPAEVPLVIAPGAWALVPTGLAL
CCCCCCCCEEECCCCCCCCCCCHHHHHHCCCEEEEECCCCCCEEECCCCCEEECCCEEE
ELPEGMEAQVRPRSGLALKFGVTVLNAPGTIDADYRGEVGVILINHGREPFTVSRGMRIA
ECCCCCCEEECCCCCEEEEECEEEEECCCCCCCCCCCCEEEEEEECCCCCEEHHCCCHHH
QMVIAGVQQITLVETGNLGETARGVGGFGSTGLDRTP
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA