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The map label for this gene is yafC [C]

Identifier: 158422321

GI number: 158422321

Start: 772946

End: 773857

Strand: Reverse

Name: yafC [C]

Synonym: AZC_0697

Alternate gene names: 158422321

Gene position: 773857-772946 (Counterclockwise)

Preceding gene: 158422323

Following gene: 158422317

Centisome position: 14.41

GC content: 71.38

Gene sequence:

>912_bases
ATGCTCAAGCTCGACGGGGTAGCCGCCTTCGTGGCGGTGGCAGACGCCGGCTCGATTTCGGAGGCGGCACGGCGGCTTGG
CCTTGCGAAATCGGTGGTCAGCGAGCGGCTGGCGGAGCTTGAGCGGCTGCTGGGCGCCCGCCTCATGCAGCGCAGCACCC
GTCGCCTCGCCCTCACCGAGGCCGGCACCGCCTTCCTCACCCGCGGGCGCCGCCTGCTGCGCGAGGCGGAGGAGGCGGTG
GCCGAGATCGCCGAGCAGAAAAGCCTCCTCGCCGGCCCCTTGCGGGTGTCCGCGCCCGTCAGCTTCGGCACGCTGCATCT
CGGCCGGGCGCTTCACCCCTTTCTCGCCGCCCATCCCGGCATCGAGCTGACGCTGGAACTGGACGACCGCTTCGTGGATG
TGGTGACGGATGGCTTCGATCTGGTGATCCGCCACGGGCAGGTGCATGACACGCGGCTCGCCGCCCGCCGGCTCGCCACC
AGCCGGCGCTTTCTCGTGGCCGCCCCCGCCTATCTGGAAGCTCATGAAGCGCCGGCGAGCCTCGACGCCCTTTCGGCCCA
CCGCGCCATCCTCTACGCCAATCGCGAGACCGACTGGCGCTTTGAAACACCCTCCGGGGCGGTGGCGGTGCGTCCGCGTC
CCGGCCTTCGGGTGAACAACGGCGTGATGATGCGCGGCGCCGCCGTGGCCGGGCTCGGCCTCGCGCTCCTGCCGACCTTC
CTCTGTCATGAGGAACTGGCGGCGGGGACGCTGAAGGTGGTGGATGTGGGCGTGCCAGCGGAAGGCGCACAACTGCACGC
GGCCTATCCGCTGGACCGTTCCGCCTCCGCCAAGCTGCGCGCGCTCATCGAGCACCTGCGCCGCGCCTTCGGCAGCCCGC
CCTTCTGGGAGAAGGGCCTGCGGCTGCCATAG

Upstream 100 bases:

>100_bases
GCGTGGCGGGACGACCGTGTCTCTGGACAGGAGAATGGCAAAGCTCCATTGTCCGGGTTAGCCGGCAGAAGGCGAAACCC
GTTTACGGATTATTCGAACA

Downstream 100 bases:

>100_bases
CGACAGGCCATCACCCGAGTTCCAGCGTGGTGATGCCGTAGATGGCATCGAGCGGCAGAACGGGCGCATCGCCCTTGTAC
ATGCGGGCGGTCTCGAACAC

Product: transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MLKLDGVAAFVAVADAGSISEAARRLGLAKSVVSERLAELERLLGARLMQRSTRRLALTEAGTAFLTRGRRLLREAEEAV
AEIAEQKSLLAGPLRVSAPVSFGTLHLGRALHPFLAAHPGIELTLELDDRFVDVVTDGFDLVIRHGQVHDTRLAARRLAT
SRRFLVAAPAYLEAHEAPASLDALSAHRAILYANRETDWRFETPSGAVAVRPRPGLRVNNGVMMRGAAVAGLGLALLPTF
LCHEELAAGTLKVVDVGVPAEGAQLHAAYPLDRSASAKLRALIEHLRRAFGSPPFWEKGLRLP

Sequences:

>Translated_303_residues
MLKLDGVAAFVAVADAGSISEAARRLGLAKSVVSERLAELERLLGARLMQRSTRRLALTEAGTAFLTRGRRLLREAEEAV
AEIAEQKSLLAGPLRVSAPVSFGTLHLGRALHPFLAAHPGIELTLELDDRFVDVVTDGFDLVIRHGQVHDTRLAARRLAT
SRRFLVAAPAYLEAHEAPASLDALSAHRAILYANRETDWRFETPSGAVAVRPRPGLRVNNGVMMRGAAVAGLGLALLPTF
LCHEELAAGTLKVVDVGVPAEGAQLHAAYPLDRSASAKLRALIEHLRRAFGSPPFWEKGLRLP
>Mature_303_residues
MLKLDGVAAFVAVADAGSISEAARRLGLAKSVVSERLAELERLLGARLMQRSTRRLALTEAGTAFLTRGRRLLREAEEAV
AEIAEQKSLLAGPLRVSAPVSFGTLHLGRALHPFLAAHPGIELTLELDDRFVDVVTDGFDLVIRHGQVHDTRLAARRLAT
SRRFLVAAPAYLEAHEAPASLDALSAHRAILYANRETDWRFETPSGAVAVRPRPGLRVNNGVMMRGAAVAGLGLALLPTF
LCHEELAAGTLKVVDVGVPAEGAQLHAAYPLDRSASAKLRALIEHLRRAFGSPPFWEKGLRLP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=30.7432432432432, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=31.5068493150685, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=32.4137931034483, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=29.8657718120805, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=26.7123287671233, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=31.25, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=25.6849315068493, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786448, Length=258, Percent_Identity=29.8449612403101, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1789173, Length=249, Percent_Identity=27.710843373494, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI157672245, Length=177, Percent_Identity=31.0734463276836, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=25.390625, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32661; Mature: 32661

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50931 HTH_LYSR ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLDGVAAFVAVADAGSISEAARRLGLAKSVVSERLAELERLLGARLMQRSTRRLALTE
CCEECHHEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHH
AGTAFLTRGRRLLREAEEAVAEIAEQKSLLAGPLRVSAPVSFGTLHLGRALHPFLAAHPG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCC
IELTLELDDRFVDVVTDGFDLVIRHGQVHDTRLAARRLATSRRFLVAAPAYLEAHEAPAS
CEEEEEECCCEEHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCH
LDALSAHRAILYANRETDWRFETPSGAVAVRPRPGLRVNNGVMMRGAAVAGLGLALLPTF
HHHHHHCEEEEEECCCCCEEEECCCCCEEECCCCCCEECCCEEEECHHHHHHHHHHHHHH
LCHEELAAGTLKVVDVGVPAEGAQLHAAYPLDRSASAKLRALIEHLRRAFGSPPFWEKGL
HHHHHHHCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCC
RLP
CCC
>Mature Secondary Structure
MLKLDGVAAFVAVADAGSISEAARRLGLAKSVVSERLAELERLLGARLMQRSTRRLALTE
CCEECHHEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHH
AGTAFLTRGRRLLREAEEAVAEIAEQKSLLAGPLRVSAPVSFGTLHLGRALHPFLAAHPG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCC
IELTLELDDRFVDVVTDGFDLVIRHGQVHDTRLAARRLATSRRFLVAAPAYLEAHEAPAS
CEEEEEECCCEEHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCH
LDALSAHRAILYANRETDWRFETPSGAVAVRPRPGLRVNNGVMMRGAAVAGLGLALLPTF
HHHHHHCEEEEEECCCCCEEEECCCCCEEECCCCCCEECCCEEEECHHHHHHHHHHHHHH
LCHEELAAGTLKVVDVGVPAEGAQLHAAYPLDRSASAKLRALIEHLRRAFGSPPFWEKGL
HHHHHHHCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCC
RLP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]