Click here to switch to the map view.

The map label for this gene is prpB [C]

Identifier: 158422311

GI number: 158422311

Start: 759244

End: 760083

Strand: Reverse

Name: prpB [C]

Synonym: AZC_0687

Alternate gene names: 158422311

Gene position: 760083-759244 (Counterclockwise)

Preceding gene: 158422312

Following gene: 158422310

Centisome position: 14.15

GC content: 71.19

Gene sequence:

>840_bases
GTGTCCGAGCAGATTGCCAAGTTCGAGCGCTTCAAGGCGCTGCACCAGCAGGCCCAGGCCTTCGTCATCCCCAACCCGTG
GGATGCCGGGTCCGCGCGCCTTCTTGCCAGCCTCGGCTTCGAGGCGCTGGCCACCACCAGCGCCGGCTACGCCTTCTCCA
AGGGCAAGCGGGATTCCGCCGCGAGCCTGACGCGGGGCGAGATTCTGGAGAATGCTTCCGAGATCGTCCGCGCCACCCAC
CTGCCCGTCTCGGCCGATCTGGAGGACGGCTTCGGCGCGGCGCCCGACGCCTGCGCGGAAACGATCCGCCTCGCCTGCGC
CATCGGCCTCGTGGGCGGCTCTATCGAGGATGCGACGGGCGATCCCGCGACGCCCATCTATGATCTGGCGCACGCGATCC
AGCGCGTGGAGGCCGCGGCCGAGGCGGCGCGCGACCTGCCCTTCCTGCTGACGGCGCGGGCGGAGAACTTCCTGTGCGGC
CGGCCGGACCTCGACGACACCATCCGCCGGCTCCAGGCCTTTTCTGCCGCCGGCGCCCATGTGCTCTACGCGCCCGGCCT
GCCGAACCTCGAGGCGATCCGAACCGTCTGCGCCGCCGTGGACAAGCCCGTGAATGTCGTGATGGGGCTGCGCGGCCCCT
CCTATTCCGTCGCCGAACTCTCGGCGGCCGGCGTGCGCCGGGTGAGCGTCGGCGGCTCCTTCGCGCGGGCCGCGCTCGGC
GCCCTGATGCGGGCGGCGCAGGAGGTGCGGATGTTCGGCACCTTCAGCTATGCCGCCGAAGCGCTGCCGGGAAAGGAGAT
CATCGCTCTCATGTCGGAAACCCGCCCCGAGCGCTCCTGA

Upstream 100 bases:

>100_bases
GCCCACACGGACGTCGGCGCTTTTCTCGCGACCCAGTTCAAGCTGAAGCCCTGAGACCACGGGCGGAGCCCCCCACCTCC
CCTTCGTTCTGGAGACAGGC

Downstream 100 bases:

>100_bases
GCCGCCGGTGCACCGCACAATCCTGAGGCAGTTGCTCACCCCACCGCCAGATCACTGCCCATCAGGACATCGTGCTCATT
TTTTGATCTTTCGCATTCTC

Product: carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSEQIAKFERFKALHQQAQAFVIPNPWDAGSARLLASLGFEALATTSAGYAFSKGKRDSAASLTRGEILENASEIVRATH
LPVSADLEDGFGAAPDACAETIRLACAIGLVGGSIEDATGDPATPIYDLAHAIQRVEAAAEAARDLPFLLTARAENFLCG
RPDLDDTIRRLQAFSAAGAHVLYAPGLPNLEAIRTVCAAVDKPVNVVMGLRGPSYSVAELSAAGVRRVSVGGSFARAALG
ALMRAAQEVRMFGTFSYAAEALPGKEIIALMSETRPERS

Sequences:

>Translated_279_residues
MSEQIAKFERFKALHQQAQAFVIPNPWDAGSARLLASLGFEALATTSAGYAFSKGKRDSAASLTRGEILENASEIVRATH
LPVSADLEDGFGAAPDACAETIRLACAIGLVGGSIEDATGDPATPIYDLAHAIQRVEAAAEAARDLPFLLTARAENFLCG
RPDLDDTIRRLQAFSAAGAHVLYAPGLPNLEAIRTVCAAVDKPVNVVMGLRGPSYSVAELSAAGVRRVSVGGSFARAALG
ALMRAAQEVRMFGTFSYAAEALPGKEIIALMSETRPERS
>Mature_278_residues
SEQIAKFERFKALHQQAQAFVIPNPWDAGSARLLASLGFEALATTSAGYAFSKGKRDSAASLTRGEILENASEIVRATHL
PVSADLEDGFGAAPDACAETIRLACAIGLVGGSIEDATGDPATPIYDLAHAIQRVEAAAEAARDLPFLLTARAENFLCGR
PDLDDTIRRLQAFSAAGAHVLYAPGLPNLEAIRTVCAAVDKPVNVVMGLRGPSYSVAELSAAGVRRVSVGGSFARAALGA
LMRAAQEVRMFGTFSYAAEALPGKEIIALMSETRPERS

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=248, Percent_Identity=27.4193548387097, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 29303; Mature: 29171

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQIAKFERFKALHQQAQAFVIPNPWDAGSARLLASLGFEALATTSAGYAFSKGKRDSA
CCHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHCHHHHHHCCCCCHHCCCCCCHH
ASLTRGEILENASEIVRATHLPVSADLEDGFGAAPDACAETIRLACAIGLVGGSIEDATG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
DPATPIYDLAHAIQRVEAAAEAARDLPFLLTARAENFLCGRPDLDDTIRRLQAFSAAGAH
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCE
VLYAPGLPNLEAIRTVCAAVDKPVNVVMGLRGPSYSVAELSAAGVRRVSVGGSFARAALG
EEECCCCCCHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHCCEEEECCCHHHHHHHH
ALMRAAQEVRMFGTFSYAAEALPGKEIIALMSETRPERS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEQIAKFERFKALHQQAQAFVIPNPWDAGSARLLASLGFEALATTSAGYAFSKGKRDSA
CHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHCHHHHHHCCCCCHHCCCCCCHH
ASLTRGEILENASEIVRATHLPVSADLEDGFGAAPDACAETIRLACAIGLVGGSIEDATG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
DPATPIYDLAHAIQRVEAAAEAARDLPFLLTARAENFLCGRPDLDDTIRRLQAFSAAGAH
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCE
VLYAPGLPNLEAIRTVCAAVDKPVNVVMGLRGPSYSVAELSAAGVRRVSVGGSFARAALG
EEECCCCCCHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHCCEEEECCCHHHHHHHH
ALMRAAQEVRMFGTFSYAAEALPGKEIIALMSETRPERS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]