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The map label for this gene is hpcH [H]

Identifier: 158422179

GI number: 158422179

Start: 615326

End: 616207

Strand: Reverse

Name: hpcH [H]

Synonym: AZC_0555

Alternate gene names: 158422179

Gene position: 616207-615326 (Counterclockwise)

Preceding gene: 158422180

Following gene: 158422178

Centisome position: 11.48

GC content: 69.5

Gene sequence:

>882_bases
TTGCCCCTTCCCCTTGAGCGCCGCGCGCTCAAGAATGGGCCGCCGCCGTCCGTGCGGCCCGCGGGCCATCACTGGCCCGT
CTCCGGCAACAAATCAGGAAACGCCATGCCTCAGGAAGCGATGCCGCGGAACGCCTTCAAGCGCGCCATCCACGCCGGCC
GTCAGCAGATCGGCCTCTGGTGCTCGATCCCGAGCCCGTTCACGGTGGAAGTGCTGGCCGGCTCCGGCTTCGACTGGCTG
CTGCTCGACACCGAGCACGCTCCCGCCGACATTCCCGAAATCTTCGCCCAGCTCCAGGCGGCGGCGGGCGGCAGCGCCAG
CCCCGTGGTGCGCGTGCCCTCGAACGATCCCGTGGCCATCAAGCGGGCGCTCGACGCCGGCGCCCAGAGCCTGCTCATGC
CCATGGTGCAGACGGCGGAGGAAGCCCACGCCGCCGTGGCCGCCACCCGCTATCCGCCGGAGGGTATCCGGGGCTTTGCC
GGCATGAGCCGCGCCACCCGCTTCGGCCGGGTGAAGGACTATTTCAAGCAGGCCCATGACGAGACCTGCGTGCTCGTTCA
GGTGGAGAGCGCGCAGGCGCTCGACAATCTGGAAGCCATCTGTGCGGTGGAAGGCATCGACGGCGTCTTTATCGGCCCCG
GTGATCTCTCCGCCAGCCTCGGCTTTCTCGACGGACAGGGCGCACCGGAAATGGTGAAGCTCATCGAGGAGACGCTCCGC
CGCATCGTCGCCGCCGGCAACCGGCCCGGCATCCTCACCGCCGACCGGACGCTCGCCCGCCGCTACATGGCGGCCGGCAG
CGTCTTCACCGCCGTGGATATCGACCTCGCCTTGCTGGTGCGCCAGACGGAAGCGCTGGCGGCCGAGTTCAAGGCCGGTT
GA

Upstream 100 bases:

>100_bases
AGGAGCGCGTGCGCTGGCGCGAGCTCGGCACGGCCGATCCCTATTCCGCCACCACGCGCTGAGGGCTCCGCGCGGCGTTT
CATCCCGCCGCCTCCCGGTT

Downstream 100 bases:

>100_bases
GAGCGGAGCACGCCATGAGCACCGATACCTTCAAGGCCATCGTCGCCCGCAAGGCCGAGGGCGGCCAGAGCGTGGCGCTG
GAGCAGGCGGACGAGAGCCT

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPLPLERRALKNGPPPSVRPAGHHWPVSGNKSGNAMPQEAMPRNAFKRAIHAGRQQIGLWCSIPSPFTVEVLAGSGFDWL
LLDTEHAPADIPEIFAQLQAAAGGSASPVVRVPSNDPVAIKRALDAGAQSLLMPMVQTAEEAHAAVAATRYPPEGIRGFA
GMSRATRFGRVKDYFKQAHDETCVLVQVESAQALDNLEAICAVEGIDGVFIGPGDLSASLGFLDGQGAPEMVKLIEETLR
RIVAAGNRPGILTADRTLARRYMAAGSVFTAVDIDLALLVRQTEALAAEFKAG

Sequences:

>Translated_293_residues
MPLPLERRALKNGPPPSVRPAGHHWPVSGNKSGNAMPQEAMPRNAFKRAIHAGRQQIGLWCSIPSPFTVEVLAGSGFDWL
LLDTEHAPADIPEIFAQLQAAAGGSASPVVRVPSNDPVAIKRALDAGAQSLLMPMVQTAEEAHAAVAATRYPPEGIRGFA
GMSRATRFGRVKDYFKQAHDETCVLVQVESAQALDNLEAICAVEGIDGVFIGPGDLSASLGFLDGQGAPEMVKLIEETLR
RIVAAGNRPGILTADRTLARRYMAAGSVFTAVDIDLALLVRQTEALAAEFKAG
>Mature_292_residues
PLPLERRALKNGPPPSVRPAGHHWPVSGNKSGNAMPQEAMPRNAFKRAIHAGRQQIGLWCSIPSPFTVEVLAGSGFDWLL
LDTEHAPADIPEIFAQLQAAAGGSASPVVRVPSNDPVAIKRALDAGAQSLLMPMVQTAEEAHAAVAATRYPPEGIRGFAG
MSRATRFGRVKDYFKQAHDETCVLVQVESAQALDNLEAICAVEGIDGVFIGPGDLSASLGFLDGQGAPEMVKLIEETLRR
IVAAGNRPGILTADRTLARRYMAAGSVFTAVDIDLALLVRQTEALAAEFKAG

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=256, Percent_Identity=48.4375, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1788578, Length=256, Percent_Identity=48.828125, Blast_Score=238, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 31075; Mature: 30944

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPLERRALKNGPPPSVRPAGHHWPVSGNKSGNAMPQEAMPRNAFKRAIHAGRQQIGLW
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCHHHEEEE
CSIPSPFTVEVLAGSGFDWLLLDTEHAPADIPEIFAQLQAAAGGSASPVVRVPSNDPVAI
EECCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHH
KRALDAGAQSLLMPMVQTAEEAHAAVAATRYPPEGIRGFAGMSRATRFGRVKDYFKQAHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETCVLVQVESAQALDNLEAICAVEGIDGVFIGPGDLSASLGFLDGQGAPEMVKLIEETLR
CEEEEEEECCHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RIVAAGNRPGILTADRTLARRYMAAGSVFTAVDIDLALLVRQTEALAAEFKAG
HHHHCCCCCCEEEHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLPLERRALKNGPPPSVRPAGHHWPVSGNKSGNAMPQEAMPRNAFKRAIHAGRQQIGLW
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCHHHEEEE
CSIPSPFTVEVLAGSGFDWLLLDTEHAPADIPEIFAQLQAAAGGSASPVVRVPSNDPVAI
EECCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHH
KRALDAGAQSLLMPMVQTAEEAHAAVAATRYPPEGIRGFAGMSRATRFGRVKDYFKQAHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETCVLVQVESAQALDNLEAICAVEGIDGVFIGPGDLSASLGFLDGQGAPEMVKLIEETLR
CEEEEEEECCHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RIVAAGNRPGILTADRTLARRYMAAGSVFTAVDIDLALLVRQTEALAAEFKAG
HHHHCCCCCCEEEHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA