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The map label for this gene is kefA [H]

Identifier: 158422175

GI number: 158422175

Start: 609352

End: 611868

Strand: Reverse

Name: kefA [H]

Synonym: AZC_0551

Alternate gene names: 158422175

Gene position: 611868-609352 (Counterclockwise)

Preceding gene: 158422178

Following gene: 158422174

Centisome position: 11.39

GC content: 68.22

Gene sequence:

>2517_bases
ATGCAGCGCGCTTTGGTGCGGATCGCGGTTCTGCTGACCTTGATGCTGGCGCTCGTCGCCGGCCAGAGCGTCTGGGCCGC
CCCTCCTGCCGATCCCGCGCTCGCCACCGCCCGCGAGAAGCTGGAAGCCAACCGCTCCGTGCTCGATGCCGTCGAGGCCT
CGCTGGGCGTGGACGGCCTGCGGGCGCCCGATCTCGATGAGCTGCGCTCCCGCCTCGATCCGGTCCGCCGCGAGATGCAG
GCGCGTTCCGAAATGCTCGGCCCGCGCGCCACCGACGTGAAGACCCGTCTCGACGAACTCGGCCCGGTGCCGGCGGCCGG
TGCGGCGCCCGAAGATCCCAATGTGACCGCGGACCGCGAGCGGCTGAAGGTGCTCTCCACCGACATCGACGCGCTGGTGA
AGCAGAACCGTGCCCTCGCCGCGCGCGCCGACCAGCTCTCAGACCGCATCAGCACCCGGCGGCGGGAATTGTTCACCGGC
CAGCTCTTCGAGCGGTCCATCTCCATCGTGGACCCCAGCCTGTGGGGCACCGGCATGGCGGCGGTGCGCTCCGCGCTCCG
CAGCATGTCGTTGCTGGTGAGCGATTCCTACGGCTACGCCCGCCAGCGGCAGGACACGCTGACGCTCATCGCGATCGGTG
CCGGCATGGCCCTCATCGTGGGGCTCATCGTCTTCCTTGGTCGGCTTCTGCGGCGGCGCCTCAGCAGCCACGCGCCGGCG
GACGGCGAGCGGATGCCGCGCCTGCGCGCCTCCCGCGAGGCGGTGAAGACCGCCGTCTTCGAAAGCCTCGTCGGCCCGCT
GGCGGCGCTCGCCGCCGTCAATTTCGCCAGCGGCTTCGAGGTCGTGCCGGCCCGCGTCGAGGAAATCCTGCGCGGGCTCA
TGGTGGCCATCTTCATCTATGCGCTCGGCCGCGCGGTCGCCCGCGCCGTCCTGGCGCCGAGCCAGCCCTGGCGGCGCCTG
CCTCCGCTGTCCGACACGGCGGCGGCCACCATCTTCCGCTTCTTCTCCTGGTCGGTGACCACGCTCGCCATCGGCTCCTT
CGTGAATGCGCTCAACCGCGCCCTGTTCACGCCGGTGGCCCTCACCGTCCTCGTCTCCGGCATCATCGTCATCTTCATCA
GCGCCTTCACCGCCCTGATGCTGGTGCGCATCGGGCGCATCGCCGAGGAGGAAGAGGCACGCGACACCGCCCTCGCCGAG
AGCGGCGGCACGCCCTCCGGCAACCTCGACCTCAACGGCGCGCCGTGGATCCGCACGCTGCTCTGGCTCATCATCGCCGT
GCTCGTGGCCTCGCTGGTCGCCGGCTATGTGAGCTTCGCGGTCTTCGTGGCCTCCCGCATGCTGGTGGCGGCGGTCGTCC
TCAGCGCGCTCTACATCATCTATGCGCTGATTGACGCCTTCTTCGGCGAGGGCCTCTCGGCGGAAACCCACCGGGCGCGG
GTCTTCTCCAAGACCCTCGGCCTGCGGCCCAAGAGCCTTGAGGTCATCGCGACCCTCGTCTCCGGCCTGCTGAAGCTGTG
CCTGTTCATCGTCGCGCTGCTGCTGATGGCGGGAAGCTGGGGCACCTCCTCGGCGGACATCCAGGAGACCGTCGAGCGCG
TCACCTTCGGCATCCACATCGGCAACACCACGATCACGCTCTGGAACGTGGTCTATGCGGTGACGCTGCTCATCATCGGC
GTACTGCTCGCCCGCGGCCTGCAGAAGTGGATGGCGACGAAACTGCTGCCGGGCACCGGCATCGAGGCCAGTCTCCAGAC
CTCCATCTCCACCATCATCGGCTATGTGGGCACCATCATCGCCATCATGGTGGCGGCGGGCGAGATCGGGCTCAACCTCC
AGAACATCGCCCTCGTCGCCGGTGCCCTCTCGGTCGGTATCGGTTTCGGCCTCCAATCCATCATCTCCAACTTCGTGTCC
GGCCTCATCCTTCTCACAGAGCGCCCCATCCGCGTGGGCGACACCATTAATGTGAAGGGCGAGGAAGGCTATGTGCGGCG
CATCAGCGTGCGTTCCACCGAGATCGAGACATTCGAGCGGGCGACCGTCATCGTCCCGAACTCGGACCTCATCACCGGCA
TGGTGAAGAACTGGACGCACTCCAACACCACCGGGCGCGTCATCGTGGCGGTGAACATCGCCTATGACGCGGACGTGGAA
GAGGTGCGGGACATTCTGGTCGCCTGCGCCTGCGACCATCCGCAGGTGCTCCAGACGCCGCCGCCGCGGGTCTTCATCAC
CAAGTTCGCCGACACCGGAATCGTCTTCGAGCTGCGCTGTGTGGTGGCGAACGTGGACTATTCGCTCACCGTGAAGAGCG
ACCTCCACTTCTCCATCCTGCGGCGCTTCCGCAAGCACGACATCGTGATGACCAGCCAGCCCTGGGCGGCGCTGAGCCGC
GCCCCGGCCGACCTCGTGCCGCCGCATGTGGACGCAGATCCGGAGCCGCCGGCGGTGGAAGCCAAGCCCTCCACACCCGT
CAAGGGCGGCGGCTCCATCAGCGATTCCACCGGCTGA

Upstream 100 bases:

>100_bases
CGGCACTTGCTTTTTGCGGGCCGCTCCCAATGCTGCGGGCGCAAAGTGAAGCGGCGCAGTCCCGCGCCGATCTGGCCTCT
CAGCCCCGGAGACAGTCCTG

Downstream 100 bases:

>100_bases
GCCGGCCCCGCCTGCGATAACCCTTCCTCAACCCTCCGGGCGCATAGAGGCCCCATGAGCGATCTCGGTCTTTCCCCCTT
CGCGGGCGCCCGCGCCCTCA

Product: transmembrane protein precursor

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 838; Mature: 838

Protein sequence:

>838_residues
MQRALVRIAVLLTLMLALVAGQSVWAAPPADPALATAREKLEANRSVLDAVEASLGVDGLRAPDLDELRSRLDPVRREMQ
ARSEMLGPRATDVKTRLDELGPVPAAGAAPEDPNVTADRERLKVLSTDIDALVKQNRALAARADQLSDRISTRRRELFTG
QLFERSISIVDPSLWGTGMAAVRSALRSMSLLVSDSYGYARQRQDTLTLIAIGAGMALIVGLIVFLGRLLRRRLSSHAPA
DGERMPRLRASREAVKTAVFESLVGPLAALAAVNFASGFEVVPARVEEILRGLMVAIFIYALGRAVARAVLAPSQPWRRL
PPLSDTAAATIFRFFSWSVTTLAIGSFVNALNRALFTPVALTVLVSGIIVIFISAFTALMLVRIGRIAEEEEARDTALAE
SGGTPSGNLDLNGAPWIRTLLWLIIAVLVASLVAGYVSFAVFVASRMLVAAVVLSALYIIYALIDAFFGEGLSAETHRAR
VFSKTLGLRPKSLEVIATLVSGLLKLCLFIVALLLMAGSWGTSSADIQETVERVTFGIHIGNTTITLWNVVYAVTLLIIG
VLLARGLQKWMATKLLPGTGIEASLQTSISTIIGYVGTIIAIMVAAGEIGLNLQNIALVAGALSVGIGFGLQSIISNFVS
GLILLTERPIRVGDTINVKGEEGYVRRISVRSTEIETFERATVIVPNSDLITGMVKNWTHSNTTGRVIVAVNIAYDADVE
EVRDILVACACDHPQVLQTPPPRVFITKFADTGIVFELRCVVANVDYSLTVKSDLHFSILRRFRKHDIVMTSQPWAALSR
APADLVPPHVDADPEPPAVEAKPSTPVKGGGSISDSTG

Sequences:

>Translated_838_residues
MQRALVRIAVLLTLMLALVAGQSVWAAPPADPALATAREKLEANRSVLDAVEASLGVDGLRAPDLDELRSRLDPVRREMQ
ARSEMLGPRATDVKTRLDELGPVPAAGAAPEDPNVTADRERLKVLSTDIDALVKQNRALAARADQLSDRISTRRRELFTG
QLFERSISIVDPSLWGTGMAAVRSALRSMSLLVSDSYGYARQRQDTLTLIAIGAGMALIVGLIVFLGRLLRRRLSSHAPA
DGERMPRLRASREAVKTAVFESLVGPLAALAAVNFASGFEVVPARVEEILRGLMVAIFIYALGRAVARAVLAPSQPWRRL
PPLSDTAAATIFRFFSWSVTTLAIGSFVNALNRALFTPVALTVLVSGIIVIFISAFTALMLVRIGRIAEEEEARDTALAE
SGGTPSGNLDLNGAPWIRTLLWLIIAVLVASLVAGYVSFAVFVASRMLVAAVVLSALYIIYALIDAFFGEGLSAETHRAR
VFSKTLGLRPKSLEVIATLVSGLLKLCLFIVALLLMAGSWGTSSADIQETVERVTFGIHIGNTTITLWNVVYAVTLLIIG
VLLARGLQKWMATKLLPGTGIEASLQTSISTIIGYVGTIIAIMVAAGEIGLNLQNIALVAGALSVGIGFGLQSIISNFVS
GLILLTERPIRVGDTINVKGEEGYVRRISVRSTEIETFERATVIVPNSDLITGMVKNWTHSNTTGRVIVAVNIAYDADVE
EVRDILVACACDHPQVLQTPPPRVFITKFADTGIVFELRCVVANVDYSLTVKSDLHFSILRRFRKHDIVMTSQPWAALSR
APADLVPPHVDADPEPPAVEAKPSTPVKGGGSISDSTG
>Mature_838_residues
MQRALVRIAVLLTLMLALVAGQSVWAAPPADPALATAREKLEANRSVLDAVEASLGVDGLRAPDLDELRSRLDPVRREMQ
ARSEMLGPRATDVKTRLDELGPVPAAGAAPEDPNVTADRERLKVLSTDIDALVKQNRALAARADQLSDRISTRRRELFTG
QLFERSISIVDPSLWGTGMAAVRSALRSMSLLVSDSYGYARQRQDTLTLIAIGAGMALIVGLIVFLGRLLRRRLSSHAPA
DGERMPRLRASREAVKTAVFESLVGPLAALAAVNFASGFEVVPARVEEILRGLMVAIFIYALGRAVARAVLAPSQPWRRL
PPLSDTAAATIFRFFSWSVTTLAIGSFVNALNRALFTPVALTVLVSGIIVIFISAFTALMLVRIGRIAEEEEARDTALAE
SGGTPSGNLDLNGAPWIRTLLWLIIAVLVASLVAGYVSFAVFVASRMLVAAVVLSALYIIYALIDAFFGEGLSAETHRAR
VFSKTLGLRPKSLEVIATLVSGLLKLCLFIVALLLMAGSWGTSSADIQETVERVTFGIHIGNTTITLWNVVYAVTLLIIG
VLLARGLQKWMATKLLPGTGIEASLQTSISTIIGYVGTIIAIMVAAGEIGLNLQNIALVAGALSVGIGFGLQSIISNFVS
GLILLTERPIRVGDTINVKGEEGYVRRISVRSTEIETFERATVIVPNSDLITGMVKNWTHSNTTGRVIVAVNIAYDADVE
EVRDILVACACDHPQVLQTPPPRVFITKFADTGIVFELRCVVANVDYSLTVKSDLHFSILRRFRKHDIVMTSQPWAALSR
APADLVPPHVDADPEPPAVEAKPSTPVKGGGSISDSTG

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=246, Percent_Identity=35.7723577235772, Blast_Score=167, Evalue=3e-42,
Organism=Escherichia coli, GI2367355, Length=257, Percent_Identity=35.408560311284, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1789291, Length=249, Percent_Identity=24.8995983935743, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787591, Length=217, Percent_Identity=26.2672811059908, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 89955; Mature: 89955

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRALVRIAVLLTLMLALVAGQSVWAAPPADPALATAREKLEANRSVLDAVEASLGVDGL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RAPDLDELRSRLDPVRREMQARSEMLGPRATDVKTRLDELGPVPAAGAAPEDPNVTADRE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
RLKVLSTDIDALVKQNRALAARADQLSDRISTRRRELFTGQLFERSISIVDPSLWGTGMA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
AVRSALRSMSLLVSDSYGYARQRQDTLTLIAIGAGMALIVGLIVFLGRLLRRRLSSHAPA
HHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGERMPRLRASREAVKTAVFESLVGPLAALAAVNFASGFEVVPARVEEILRGLMVAIFIY
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ALGRAVARAVLAPSQPWRRLPPLSDTAAATIFRFFSWSVTTLAIGSFVNALNRALFTPVA
HHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVLVSGIIVIFISAFTALMLVRIGRIAEEEEARDTALAESGGTPSGNLDLNGAPWIRTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LWLIIAVLVASLVAGYVSFAVFVASRMLVAAVVLSALYIIYALIDAFFGEGLSAETHRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
VFSKTLGLRPKSLEVIATLVSGLLKLCLFIVALLLMAGSWGTSSADIQETVERVTFGIHI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEE
GNTTITLWNVVYAVTLLIIGVLLARGLQKWMATKLLPGTGIEASLQTSISTIIGYVGTII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
AIMVAAGEIGLNLQNIALVAGALSVGIGFGLQSIISNFVSGLILLTERPIRVGDTINVKG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCEEEECC
EEGYVRRISVRSTEIETFERATVIVPNSDLITGMVKNWTHSNTTGRVIVAVNIAYDADVE
CCCCEEEEEECHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHH
EVRDILVACACDHPQVLQTPPPRVFITKFADTGIVFELRCVVANVDYSLTVKSDLHFSIL
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCCEEEEECCCHHHHHH
RRFRKHDIVMTSQPWAALSRAPADLVPPHVDADPEPPAVEAKPSTPVKGGGSISDSTG
HHHHHCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQRALVRIAVLLTLMLALVAGQSVWAAPPADPALATAREKLEANRSVLDAVEASLGVDGL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RAPDLDELRSRLDPVRREMQARSEMLGPRATDVKTRLDELGPVPAAGAAPEDPNVTADRE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
RLKVLSTDIDALVKQNRALAARADQLSDRISTRRRELFTGQLFERSISIVDPSLWGTGMA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
AVRSALRSMSLLVSDSYGYARQRQDTLTLIAIGAGMALIVGLIVFLGRLLRRRLSSHAPA
HHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGERMPRLRASREAVKTAVFESLVGPLAALAAVNFASGFEVVPARVEEILRGLMVAIFIY
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ALGRAVARAVLAPSQPWRRLPPLSDTAAATIFRFFSWSVTTLAIGSFVNALNRALFTPVA
HHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVLVSGIIVIFISAFTALMLVRIGRIAEEEEARDTALAESGGTPSGNLDLNGAPWIRTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LWLIIAVLVASLVAGYVSFAVFVASRMLVAAVVLSALYIIYALIDAFFGEGLSAETHRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
VFSKTLGLRPKSLEVIATLVSGLLKLCLFIVALLLMAGSWGTSSADIQETVERVTFGIHI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEE
GNTTITLWNVVYAVTLLIIGVLLARGLQKWMATKLLPGTGIEASLQTSISTIIGYVGTII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
AIMVAAGEIGLNLQNIALVAGALSVGIGFGLQSIISNFVSGLILLTERPIRVGDTINVKG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCEEEECC
EEGYVRRISVRSTEIETFERATVIVPNSDLITGMVKNWTHSNTTGRVIVAVNIAYDADVE
CCCCEEEEEECHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHH
EVRDILVACACDHPQVLQTPPPRVFITKFADTGIVFELRCVVANVDYSLTVKSDLHFSIL
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCCEEEEECCCHHHHHH
RRFRKHDIVMTSQPWAALSRAPADLVPPHVDADPEPPAVEAKPSTPVKGGGSISDSTG
HHHHHCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]