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The map label for this gene is aes [H]

Identifier: 158422169

GI number: 158422169

Start: 600571

End: 602040

Strand: Reverse

Name: aes [H]

Synonym: AZC_0545

Alternate gene names: 158422169

Gene position: 602040-600571 (Counterclockwise)

Preceding gene: 158422170

Following gene: 158422166

Centisome position: 11.21

GC content: 70.41

Gene sequence:

>1470_bases
GTGCGGGCGGTCGCGGTGCTCGATCAGATAGATGCCCTGCCATGTGCCGAGACGCGGCCGCCCGGCGAAGACCGGAATGG
CGAGCGTGCTGTCCGAGAGCAGCGTGCGCACATGGGCGGGCATGTCGTCCGGCCCCTCCGTCGTGTGCACCCAGCCGCGA
TTGCGCGGGGCGAGGTCGTTCAGCGCGGTGATGAGGTCCGTCTGCACGTCCGGGTCCGCATTCTCCTGAATGGTGAGGGA
CGCCGAGGTGTGCCGGCAGAAGACCGTAAGCACGCCCTGCCCGGCGCCGATCTCCGAGAGGAAGGCCGTGGCCTCCCGCG
TGATGTCGATGAAGCCGGGACCGCGCGTCGGCACCTCCAGCTCGCACTGGGCCTGGAAATCCACCGGCCCGCTCACCGCC
GCGCTACGCCTCATCTCATGTCCTTTCCACCGCATGACCAAGGGGCAGCTTGCCAGCCGGGCGCGCGATGCCCACCTTGC
GGCCGCATGTTCCAGCCCCCCGGCCCCCGGAGCCCGATGACCGTTCTCTCCATCGACCCGCACCTGTTCACCGATGCCGC
CGTGGCGCCGGACACGAAAATGGTCAATGCCGCCATCGTCAAGGCGCTCTCCCGGGCGCCGGATCTCGGCCTCGTGCCCT
GGGCGGTGGTGCGGCAGGCCCGGCTCTCCGGCAAGGGCACCTTTCCTTTGCCGCCGGAAAGCCCGCGTGCCCGCAACGAG
ACCATCGAGGGTCCGCGCGGCCCGGTGCCGGTGCGCATCTATCTGCCCGACGGCGCACCCACGGGCGTCTATCTGCATTT
CCATGGCGGCGGCTGGACCATCGGCTCGGCGCGGGAGAATGACGGGCTGAATGACGCGGTGGCGGCCGCCGGCCTCGTGG
TGGTGTCGGTGGACTATGCCCTCGCCCCCGAGACGCCCTATCCGGCGGCGCCCGATGATTGCGAGGCGGCGGCCCTCTGG
CTGCTGCGGGAGGGCCTGTCGCTGTTCGGGACGGACCGCTTCCTCATCGGCGGAGAATCGGCCGGCGCGCATCTCGCCGT
CACCACCCTGCTGCGGCTGCGCGACCGGCACGGCATCTCGCCTTTCGTGGCGGCGAGCCTCCACGCCGGCTGCTACGACC
TGCGCCTCACCCCCAGCGCCCGCAACTGGGGCACCGAGCCCCTGGTGCTGAACACGGCCGATATCGAGCGCTTCGTGCGC
AACTATCTGCGGCTGGAGGGCGAAGCCGAGCATCCGGATGTCTCGCCTTTGCTGGCAGACCTGAAGGGCCTGCCCAAGGC
GCAATTCCTCGTCGGCACCCGCGATCCGCTGCTGGACGACACGCTGTTCATGGCCAACCGCTGGCTCGCCAGCGGCAACC
CGGCGCGGCTTGAGGCGTTTCCCGGCGGGTGCCACGTCTTCATGGGCTTCGTCGGCTCGCTGGCCGACAAGGGCCGGGCG
CGGATCGTGGACTTCCTGCGCGACGCCTGA

Upstream 100 bases:

>100_bases
TGTGCAGGACAGCGCAGTGAAAGGCACCTTCTCGCTGCGCTTCTGAGCGCCCTCAGCCCCGCGCCGCCTGCCCGAGAAAG
CCCAGAATGATCTGGCGCCG

Downstream 100 bases:

>100_bases
TCAGCGGCTGCCGTTCTCGACCCCGTCGATGAAGCCGCGCAGGCGCTGGTAGAGCTTGCCCGCATAGGCATAAGCGGCGT
CGAGATCGGACTGCGAAGGC

Product: esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MRAVAVLDQIDALPCAETRPPGEDRNGERAVREQRAHMGGHVVRPLRRVHPAAIARGEVVQRGDEVRLHVRVRILLNGEG
RRGVPAEDRKHALPGADLREEGRGLPRDVDEAGTARRHLQLALGLEIHRPAHRRATPHLMSFPPHDQGAACQPGARCPPC
GRMFQPPGPRSPMTVLSIDPHLFTDAAVAPDTKMVNAAIVKALSRAPDLGLVPWAVVRQARLSGKGTFPLPPESPRARNE
TIEGPRGPVPVRIYLPDGAPTGVYLHFHGGGWTIGSARENDGLNDAVAAAGLVVVSVDYALAPETPYPAAPDDCEAAALW
LLREGLSLFGTDRFLIGGESAGAHLAVTTLLRLRDRHGISPFVAASLHAGCYDLRLTPSARNWGTEPLVLNTADIERFVR
NYLRLEGEAEHPDVSPLLADLKGLPKAQFLVGTRDPLLDDTLFMANRWLASGNPARLEAFPGGCHVFMGFVGSLADKGRA
RIVDFLRDA

Sequences:

>Translated_489_residues
MRAVAVLDQIDALPCAETRPPGEDRNGERAVREQRAHMGGHVVRPLRRVHPAAIARGEVVQRGDEVRLHVRVRILLNGEG
RRGVPAEDRKHALPGADLREEGRGLPRDVDEAGTARRHLQLALGLEIHRPAHRRATPHLMSFPPHDQGAACQPGARCPPC
GRMFQPPGPRSPMTVLSIDPHLFTDAAVAPDTKMVNAAIVKALSRAPDLGLVPWAVVRQARLSGKGTFPLPPESPRARNE
TIEGPRGPVPVRIYLPDGAPTGVYLHFHGGGWTIGSARENDGLNDAVAAAGLVVVSVDYALAPETPYPAAPDDCEAAALW
LLREGLSLFGTDRFLIGGESAGAHLAVTTLLRLRDRHGISPFVAASLHAGCYDLRLTPSARNWGTEPLVLNTADIERFVR
NYLRLEGEAEHPDVSPLLADLKGLPKAQFLVGTRDPLLDDTLFMANRWLASGNPARLEAFPGGCHVFMGFVGSLADKGRA
RIVDFLRDA
>Mature_489_residues
MRAVAVLDQIDALPCAETRPPGEDRNGERAVREQRAHMGGHVVRPLRRVHPAAIARGEVVQRGDEVRLHVRVRILLNGEG
RRGVPAEDRKHALPGADLREEGRGLPRDVDEAGTARRHLQLALGLEIHRPAHRRATPHLMSFPPHDQGAACQPGARCPPC
GRMFQPPGPRSPMTVLSIDPHLFTDAAVAPDTKMVNAAIVKALSRAPDLGLVPWAVVRQARLSGKGTFPLPPESPRARNE
TIEGPRGPVPVRIYLPDGAPTGVYLHFHGGGWTIGSARENDGLNDAVAAAGLVVVSVDYALAPETPYPAAPDDCEAAALW
LLREGLSLFGTDRFLIGGESAGAHLAVTTLLRLRDRHGISPFVAASLHAGCYDLRLTPSARNWGTEPLVLNTADIERFVR
NYLRLEGEAEHPDVSPLLADLKGLPKAQFLVGTRDPLLDDTLFMANRWLASGNPARLEAFPGGCHVFMGFVGSLADKGRA
RIVDFLRDA

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=238, Percent_Identity=27.3109243697479, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17567059, Length=264, Percent_Identity=26.8939393939394, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=125, Percent_Identity=36, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 52850; Mature: 52850

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVAVLDQIDALPCAETRPPGEDRNGERAVREQRAHMGGHVVRPLRRVHPAAIARGEVV
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHCCHHH
QRGDEVRLHVRVRILLNGEGRRGVPAEDRKHALPGADLREEGRGLPRDVDEAGTARRHLQ
HCCCEEEEEEEEEEEECCCCCCCCCCCCHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHEE
LALGLEIHRPAHRRATPHLMSFPPHDQGAACQPGARCPPCGRMFQPPGPRSPMTVLSIDP
EEECEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
HLFTDAAVAPDTKMVNAAIVKALSRAPDLGLVPWAVVRQARLSGKGTFPLPPESPRARNE
CHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
TIEGPRGPVPVRIYLPDGAPTGVYLHFHGGGWTIGSARENDGLNDAVAAAGLVVVSVDYA
CCCCCCCCEEEEEEECCCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHHCEEEEEECCE
LAPETPYPAAPDDCEAAALWLLREGLSLFGTDRFLIGGESAGAHLAVTTLLRLRDRHGIS
ECCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
PFVAASLHAGCYDLRLTPSARNWGTEPLVLNTADIERFVRNYLRLEGEAEHPDVSPLLAD
HHHHHHHCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LKGLPKAQFLVGTRDPLLDDTLFMANRWLASGNPARLEAFPGGCHVFMGFVGSLADKGRA
HHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCHH
RIVDFLRDA
HHHHHHHCC
>Mature Secondary Structure
MRAVAVLDQIDALPCAETRPPGEDRNGERAVREQRAHMGGHVVRPLRRVHPAAIARGEVV
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHCCHHH
QRGDEVRLHVRVRILLNGEGRRGVPAEDRKHALPGADLREEGRGLPRDVDEAGTARRHLQ
HCCCEEEEEEEEEEEECCCCCCCCCCCCHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHEE
LALGLEIHRPAHRRATPHLMSFPPHDQGAACQPGARCPPCGRMFQPPGPRSPMTVLSIDP
EEECEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
HLFTDAAVAPDTKMVNAAIVKALSRAPDLGLVPWAVVRQARLSGKGTFPLPPESPRARNE
CHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
TIEGPRGPVPVRIYLPDGAPTGVYLHFHGGGWTIGSARENDGLNDAVAAAGLVVVSVDYA
CCCCCCCCEEEEEEECCCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHHCEEEEEECCE
LAPETPYPAAPDDCEAAALWLLREGLSLFGTDRFLIGGESAGAHLAVTTLLRLRDRHGIS
ECCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
PFVAASLHAGCYDLRLTPSARNWGTEPLVLNTADIERFVRNYLRLEGEAEHPDVSPLLAD
HHHHHHHCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LKGLPKAQFLVGTRDPLLDDTLFMANRWLASGNPARLEAFPGGCHVFMGFVGSLADKGRA
HHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCHH
RIVDFLRDA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA