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The map label for this gene is purF [H]

Identifier: 158422159

GI number: 158422159

Start: 592879

End: 594441

Strand: Direct

Name: purF [H]

Synonym: AZC_0535

Alternate gene names: 158422159

Gene position: 592879-594441 (Clockwise)

Preceding gene: 158422158

Following gene: 158422160

Centisome position: 11.04

GC content: 68.07

Gene sequence:

>1563_bases
ATGGATTTGGACACCGCCGCCCCGGGGGGCAAACCGTTGCATCTGGAAGACCCGCACGAGGTCGCCCGACATCACGCCAC
CTACGAGCCCGCCGCGGCCGGCTCGCGGGAGGACACCATCGACCTCGGCCTCGACGGCGATACGCTGCGCGAGGAATGCG
GCGTGTTCGGCATCTTCAACCATCCCGATGCCGCCGCCATCACCGCCATCGGCCTGCATGCGCTCCAGCATCGCGGCCAG
GAAGCCGCAGGCATCGTCACCTATGACGGCCGGCGCTTCCATTCCGAGCGGCGCCTCGGCCTCGTGGGCGACACCTTCTC
CGACAGCCGCGTGATCGAGCGCCTGCCGGGCCACATCGCGGTCGGCCACACGCGCTATTCCACCACCGGCGAGACCCTGC
TGCGCAACGTGCAGCCGCTGTTCGCCGAGCTGGACGCCGGTGGCTTCGCGGTGGGGCACAACGGCAACCTCACCAACGGC
CTCACCCTGCGCAAGCAGCTGGTGCGCGACGGCGCCATCACCCAGTCCACCACGGACACCGAGGTGATCCTGCATCTGGT
CGCCCGTTCCAAGAAGAACCGCTTCATCGACCGCTTCATCGATGCCCTGCGCGCGCTGGAAGGCGCCTATGCGCTGGTGT
CGGTGACCAACAACAAGCTCATCGGCGCCCGCGATCCCCTCGGCATCCGCCCGCTGGTGCTGGGCTCGCTGGACGGCGCT
CCCATCCTCGCCTCCGAGACCTGCGCCCTCGACATCATCGGCGCGCGCTATGTGCGCGACGTGGAGAATGGCGAGGTCAT
CGTGATCGACGAGGAGGGCGTGCAGTCCTTCAAGCCGTTCCCCGAGACGGCGGCGCGTCCCTGCCTGTTCGAGTACATCT
ATTTCGCCCGTCCCGATTCGGTTGTGAGCGGCCGCTCCGTCTATCAGGTGCGCAAGGCCTTCGGCGAAGTGCTCGCCAAG
GAAGGCCCGGCCGAGGCGGACGTGGTCGTGCCGGTGCCGGATTCCGGCGTGCCGGCGGCCATCGGCTTCTCCCGCGCCTC
GGGCATCCCCTACGAGCTCGGCATCATCCGCAACCATTATGTGGGCCGGACCTTCATCCAGCCGACCCAGACGGTGCGCG
ATCAGGGCGTGCGCATGAAGCATTCCGCCAACCGTTCGGTGGTGGAAGGCAAGCGCATCGTGCTGGTGGACGACAGCCTC
GTGCGCGGCACCACCTCGGTGAAGATCGTGCAGATGATGCGCGAGGCAGGCGCCCGCGAAGTGCACTTCCGCATCGCCTC
GCCGCCGATCACCCACCCGGATTTCTACGGCATCGACACGCCCGACCGGGACAAGCTGCTCGCCGCCACCCACGATCTGG
AGAGCATGCGCCGCTATATCGGCGCCGACTCGCTCGCCTTCCTCACGGTGGAGGGCGTCTATCGCGCCCTCGGCTGCGAG
CGCGATCCGGTGCGGCCGCAGTTCGCCGACCATTATTTCACGGGCGACTACCCGACCCCGCTCACCGACAAGGCGAGCGC
GGTCGCCTCCCAGCAGCTTTCGCTGCTGGCCGAAGCGAGCTGA

Upstream 100 bases:

>100_bases
TCAGTCAAGCCGGATACCGCCGCGATGCGCGGCAAGGGTTCGAACAACTGCTCGAAAGCACGCGCGCCAGCGGCCAGTGA
GCCGCCGAGCGGAGGCATCG

Downstream 100 bases:

>100_bases
CGGATCGCCGGGCGTGCCCGGCGGCAAGCTGACCCGCTGACAGACAGGCGCGGACCGGATAGACCGGTCCGCGCCTTTTG
TGTTTCCAGTCGAGGACATC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MDLDTAAPGGKPLHLEDPHEVARHHATYEPAAAGSREDTIDLGLDGDTLREECGVFGIFNHPDAAAITAIGLHALQHRGQ
EAAGIVTYDGRRFHSERRLGLVGDTFSDSRVIERLPGHIAVGHTRYSTTGETLLRNVQPLFAELDAGGFAVGHNGNLTNG
LTLRKQLVRDGAITQSTTDTEVILHLVARSKKNRFIDRFIDALRALEGAYALVSVTNNKLIGARDPLGIRPLVLGSLDGA
PILASETCALDIIGARYVRDVENGEVIVIDEEGVQSFKPFPETAARPCLFEYIYFARPDSVVSGRSVYQVRKAFGEVLAK
EGPAEADVVVPVPDSGVPAAIGFSRASGIPYELGIIRNHYVGRTFIQPTQTVRDQGVRMKHSANRSVVEGKRIVLVDDSL
VRGTTSVKIVQMMREAGAREVHFRIASPPITHPDFYGIDTPDRDKLLAATHDLESMRRYIGADSLAFLTVEGVYRALGCE
RDPVRPQFADHYFTGDYPTPLTDKASAVASQQLSLLAEAS

Sequences:

>Translated_520_residues
MDLDTAAPGGKPLHLEDPHEVARHHATYEPAAAGSREDTIDLGLDGDTLREECGVFGIFNHPDAAAITAIGLHALQHRGQ
EAAGIVTYDGRRFHSERRLGLVGDTFSDSRVIERLPGHIAVGHTRYSTTGETLLRNVQPLFAELDAGGFAVGHNGNLTNG
LTLRKQLVRDGAITQSTTDTEVILHLVARSKKNRFIDRFIDALRALEGAYALVSVTNNKLIGARDPLGIRPLVLGSLDGA
PILASETCALDIIGARYVRDVENGEVIVIDEEGVQSFKPFPETAARPCLFEYIYFARPDSVVSGRSVYQVRKAFGEVLAK
EGPAEADVVVPVPDSGVPAAIGFSRASGIPYELGIIRNHYVGRTFIQPTQTVRDQGVRMKHSANRSVVEGKRIVLVDDSL
VRGTTSVKIVQMMREAGAREVHFRIASPPITHPDFYGIDTPDRDKLLAATHDLESMRRYIGADSLAFLTVEGVYRALGCE
RDPVRPQFADHYFTGDYPTPLTDKASAVASQQLSLLAEAS
>Mature_520_residues
MDLDTAAPGGKPLHLEDPHEVARHHATYEPAAAGSREDTIDLGLDGDTLREECGVFGIFNHPDAAAITAIGLHALQHRGQ
EAAGIVTYDGRRFHSERRLGLVGDTFSDSRVIERLPGHIAVGHTRYSTTGETLLRNVQPLFAELDAGGFAVGHNGNLTNG
LTLRKQLVRDGAITQSTTDTEVILHLVARSKKNRFIDRFIDALRALEGAYALVSVTNNKLIGARDPLGIRPLVLGSLDGA
PILASETCALDIIGARYVRDVENGEVIVIDEEGVQSFKPFPETAARPCLFEYIYFARPDSVVSGRSVYQVRKAFGEVLAK
EGPAEADVVVPVPDSGVPAAIGFSRASGIPYELGIIRNHYVGRTFIQPTQTVRDQGVRMKHSANRSVVEGKRIVLVDDSL
VRGTTSVKIVQMMREAGAREVHFRIASPPITHPDFYGIDTPDRDKLLAATHDLESMRRYIGADSLAFLTVEGVYRALGCE
RDPVRPQFADHYFTGDYPTPLTDKASAVASQQLSLLAEAS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=462, Percent_Identity=38.961038961039, Blast_Score=340, Evalue=2e-93,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=39.4957983193277, Blast_Score=304, Evalue=9e-84,
Organism=Escherichia coli, GI1790167, Length=231, Percent_Identity=32.034632034632, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=38.4133611691023, Blast_Score=304, Evalue=9e-83,
Organism=Saccharomyces cerevisiae, GI6323958, Length=503, Percent_Identity=35.7852882703777, Blast_Score=263, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24659598, Length=504, Percent_Identity=37.8968253968254, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI28573187, Length=525, Percent_Identity=37.5238095238095, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24659604, Length=474, Percent_Identity=38.1856540084388, Blast_Score=325, Evalue=5e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56514; Mature: 56514

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDTAAPGGKPLHLEDPHEVARHHATYEPAAAGSREDTIDLGLDGDTLREECGVFGIFN
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHCCEEEEEC
HPDAAAITAIGLHALQHRGQEAAGIVTYDGRRFHSERRLGLVGDTFSDSRVIERLPGHIA
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCCCCEEECCCCCCCHHHHHCCCCEE
VGHTRYSTTGETLLRNVQPLFAELDAGGFAVGHNGNLTNGLTLRKQLVRDGAITQSTTDT
ECCEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHH
EVILHLVARSKKNRFIDRFIDALRALEGAYALVSVTNNKLIGARDPLGIRPLVLGSLDGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCEEEECCCCC
PILASETCALDIIGARYVRDVENGEVIVIDEEGVQSFKPFPETAARPCLFEYIYFARPDS
CEEECCCCEEEEHHHHHHHCCCCCCEEEEECCCCHHCCCCCHHHCCHHHHHHHHHCCCCC
VVSGRSVYQVRKAFGEVLAKEGPAEADVVVPVPDSGVPAAIGFSRASGIPYELGIIRNHY
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEHHHCCCCCEEEEEHHCCC
VGRTFIQPTQTVRDQGVRMKHSANRSVVEGKRIVLVDDSLVRGTTSVKIVQMMREAGARE
CCCEECCCHHHHHHCCCEEECCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCCE
VHFRIASPPITHPDFYGIDTPDRDKLLAATHDLESMRRYIGADSLAFLTVEGVYRALGCE
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCC
RDPVRPQFADHYFTGDYPTPLTDKASAVASQQLSLLAEAS
CCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLDTAAPGGKPLHLEDPHEVARHHATYEPAAAGSREDTIDLGLDGDTLREECGVFGIFN
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHCCEEEEEC
HPDAAAITAIGLHALQHRGQEAAGIVTYDGRRFHSERRLGLVGDTFSDSRVIERLPGHIA
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCCCCEEECCCCCCCHHHHHCCCCEE
VGHTRYSTTGETLLRNVQPLFAELDAGGFAVGHNGNLTNGLTLRKQLVRDGAITQSTTDT
ECCEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHH
EVILHLVARSKKNRFIDRFIDALRALEGAYALVSVTNNKLIGARDPLGIRPLVLGSLDGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCEEEECCCCC
PILASETCALDIIGARYVRDVENGEVIVIDEEGVQSFKPFPETAARPCLFEYIYFARPDS
CEEECCCCEEEEHHHHHHHCCCCCCEEEEECCCCHHCCCCCHHHCCHHHHHHHHHCCCCC
VVSGRSVYQVRKAFGEVLAKEGPAEADVVVPVPDSGVPAAIGFSRASGIPYELGIIRNHY
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEHHHCCCCCEEEEEHHCCC
VGRTFIQPTQTVRDQGVRMKHSANRSVVEGKRIVLVDDSLVRGTTSVKIVQMMREAGARE
CCCEECCCHHHHHHCCCEEECCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCCE
VHFRIASPPITHPDFYGIDTPDRDKLLAATHDLESMRRYIGADSLAFLTVEGVYRALGCE
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCC
RDPVRPQFADHYFTGDYPTPLTDKASAVASQQLSLLAEAS
CCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA