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The map label for this gene is otsB [H]

Identifier: 158422107

GI number: 158422107

Start: 540700

End: 541485

Strand: Reverse

Name: otsB [H]

Synonym: AZC_0483

Alternate gene names: 158422107

Gene position: 541485-540700 (Counterclockwise)

Preceding gene: 158422110

Following gene: 158422106

Centisome position: 10.08

GC content: 68.96

Gene sequence:

>786_bases
ATGCATGTTACTTCGGTACCGAAGGAAGAAGGCCGTCCGTCGCGACCTGATGCGGTGCGTCTGTTTGACCCGCGCACTTG
TGCCTTCTTCCTCGATGTGGACGGAACACTGATCGACATCGCCATCCATCCCGATGCGGTGACCGTGCCGAGCGGGCTCC
TGACCACGCTCGGAACCCTGAAGACCGCCGCCGATGGTGCGCTCGCCCTGGTCAGCGGCCGCACGGTCGCCGGGCTCGAC
GCCCTCTTCTCGCCCCTGAAACTGGCGGCCGCCGGCGTCCATGGCGGCGAGATCCGCCTCACGCCCGAGGCGCCTGTTGC
CCAGCAGAGCGCCACGCTGCCGGCGGAGACCGGAACGCGCATCAGCCGTCTGGCGCGCGATCTCGACGGCGTGCTGATGG
AGGACAAGGGCAGCAGCATCGCGGTTCATTACCGCCTCGCCCCGCAGATCGGCCCCGACCTCCAGATCGCGCTGGAGCAG
ATCGTCACTGATTATCCCGGCCTGTCCCTGCTGCCGGGCCGGCTCGTGTTCGAGGTGAAGCGCGCCGGCTGCGACAAGGG
CGTGGCGGTGCGCCAGTTCATGGCGGCGCCGGGCTTTGCGGGGCGAACCCCCGTTTTCATCGGCGACGACGTGACGGACG
AGGCCGCCTTCGCTGCCGTCTCGCAGATGGGCGGCATCGCCGTCTCCGTCGGCCGCAACCTTCCCGTCGCCGATCTGGTG
CTGCCCGAAGCGCAGGACGTGCGCGCCCTGCTCGCCACCCTTGTCGCCCAACAGCCGGAGGAGTGA

Upstream 100 bases:

>100_bases
ATGACGTTCGATCATCTGTGGCGAAAGCGCGGAGAAGTTTTGGGGGAACCTTGGCGGAGGCGTCACGTTACCCGAACAGG
CTTATTCCCACGAGGCTTTC

Downstream 100 bases:

>100_bases
CCCGTGTCGCGCTTGATCGTCGTCTCCAATCGCGTTGCCGTCCCCGAGGCAGGCAAGGTCTCCGCCGGCGGACTGGCCGT
CGCCGTGAATGCGGCGCTCA

Product: trehalose-6-phosphate phophatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MHVTSVPKEEGRPSRPDAVRLFDPRTCAFFLDVDGTLIDIAIHPDAVTVPSGLLTTLGTLKTAADGALALVSGRTVAGLD
ALFSPLKLAAAGVHGGEIRLTPEAPVAQQSATLPAETGTRISRLARDLDGVLMEDKGSSIAVHYRLAPQIGPDLQIALEQ
IVTDYPGLSLLPGRLVFEVKRAGCDKGVAVRQFMAAPGFAGRTPVFIGDDVTDEAAFAAVSQMGGIAVSVGRNLPVADLV
LPEAQDVRALLATLVAQQPEE

Sequences:

>Translated_261_residues
MHVTSVPKEEGRPSRPDAVRLFDPRTCAFFLDVDGTLIDIAIHPDAVTVPSGLLTTLGTLKTAADGALALVSGRTVAGLD
ALFSPLKLAAAGVHGGEIRLTPEAPVAQQSATLPAETGTRISRLARDLDGVLMEDKGSSIAVHYRLAPQIGPDLQIALEQ
IVTDYPGLSLLPGRLVFEVKRAGCDKGVAVRQFMAAPGFAGRTPVFIGDDVTDEAAFAAVSQMGGIAVSVGRNLPVADLV
LPEAQDVRALLATLVAQQPEE
>Mature_261_residues
MHVTSVPKEEGRPSRPDAVRLFDPRTCAFFLDVDGTLIDIAIHPDAVTVPSGLLTTLGTLKTAADGALALVSGRTVAGLD
ALFSPLKLAAAGVHGGEIRLTPEAPVAQQSATLPAETGTRISRLARDLDGVLMEDKGSSIAVHYRLAPQIGPDLQIALEQ
IVTDYPGLSLLPGRLVFEVKRAGCDKGVAVRQFMAAPGFAGRTPVFIGDDVTDEAAFAAVSQMGGIAVSVGRNLPVADLV
LPEAQDVRALLATLVAQQPEE

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=237, Percent_Identity=39.662447257384, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 27216; Mature: 27216

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTSVPKEEGRPSRPDAVRLFDPRTCAFFLDVDGTLIDIAIHPDAVTVPSGLLTTLGTL
CCCCCCCHHCCCCCCCCEEEEECCCEEEEEEECCCEEEEEEECCCCEECCCHHHHHHHHH
KTAADGALALVSGRTVAGLDALFSPLKLAAAGVHGGEIRLTPEAPVAQQSATLPAETGTR
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHH
ISRLARDLDGVLMEDKGSSIAVHYRLAPQIGPDLQIALEQIVTDYPGLSLLPGRLVFEVK
HHHHHHHHCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
RAGCDKGVAVRQFMAAPGFAGRTPVFIGDDVTDEAAFAAVSQMGGIAVSVGRNLPVADLV
HCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHCCEEEECCCCCCHHHEE
LPEAQDVRALLATLVAQQPEE
CCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHVTSVPKEEGRPSRPDAVRLFDPRTCAFFLDVDGTLIDIAIHPDAVTVPSGLLTTLGTL
CCCCCCCHHCCCCCCCCEEEEECCCEEEEEEECCCEEEEEEECCCCEECCCHHHHHHHHH
KTAADGALALVSGRTVAGLDALFSPLKLAAAGVHGGEIRLTPEAPVAQQSATLPAETGTR
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHH
ISRLARDLDGVLMEDKGSSIAVHYRLAPQIGPDLQIALEQIVTDYPGLSLLPGRLVFEVK
HHHHHHHHCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
RAGCDKGVAVRQFMAAPGFAGRTPVFIGDDVTDEAAFAAVSQMGGIAVSVGRNLPVADLV
HCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHCCEEEECCCCCCHHHEE
LPEAQDVRALLATLVAQQPEE
CCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]