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The map label for this gene is livJ [C]

Identifier: 158422101

GI number: 158422101

Start: 534316

End: 535491

Strand: Reverse

Name: livJ [C]

Synonym: AZC_0477

Alternate gene names: 158422101

Gene position: 535491-534316 (Counterclockwise)

Preceding gene: 158422102

Following gene: 158422100

Centisome position: 9.97

GC content: 65.31

Gene sequence:

>1176_bases
ATGAAGAAGTCGCTGGTTTTCGGTGCAACGCTTCTCGCAGCCGTCTCGGCGGCCGCCATGGCCCGCGCGGCCGACGATGA
GCTGGTGCTCGGTTACATGATGGCCAAGAGCGGCCCTTATGTGAGCCTCGCCAACACCAACGAGATCGCGGTCGATCTCG
CTGTGGAGGAGATCAACGCCAAGGGCGGGATCAACGGCAAGAAGATCAAGGTGGTGAAGTTCGACACCGCCGGCGATCCC
AAGCAGGCCACCACCGCGCTCCGCCGCTTTGCGGAGGACGATTCCGCGCTCGCCGTCATCGGCCCGTTCTCCTCCTCCGA
GGTGCGCACCACCTTCCCCGTGGGCGAGCGCTCCGGCATCGCGCAGATGTCCATGGCCTCCTCGGCCCCCGGCCTCACCA
AGGGCTTCTCCTACGGCTTCCGCAACACCACGGACGAGGGCATCGTCCTCGACCAGGTAATGACGTCCATCAAGGACAAG
AAGCTGCCCGCCGCTTCCGGTGCCGCCGCCTATGCCACGGACGACGTGGTGTCGAAGTCCATCGGCACCCTCGTGGTGCC
CAAGCTGTTCGAGAAGTATTCCGTGCCCATGAAGGGCTCGGTGGACTTCCAGCTCGCCGCCTTCGACCTCTCCCCGCAGG
TTTCCAAGCTTAAGGAGATGGCCCCCGACATCGTCGGCCTCGGCTCGCCGCCGGAAGGCGCGGTGAACCTCGCCAAGGAG
CTGAAGCGCCAGGGCGTCTCCACCCGCGTCATCGGCGGCACCACCATCGCCGATCCCGAACTGCCCAAGCGCATGGACGG
CGCGGGCGAGAACCTCACCATCGGCACCACCTTCTTCCCCGAGGTGAACGACAAGACCAAGGCCTTCGCCGCCGAATTCG
GCAAGCGCACCAAGGCCGCCGGCCTGCCGCGCACCGAGCCGAACCAGATGGACGCCGCCGTCTATGACATCGTCTATCTC
TATGCAGAGGCGATGAAGCGCGCCGCCGTGACCGGGGACAAGGCCAAGCTCGTGGCCGAGCGCACGGGCGTGCGCGACGC
GCTCGCCAAGATGAAGGATTTCGAGGCCCTCGAGGGCAAGATCTCCTTCCGTGACGGCGATGCGGTGAAGCCGGTCTACA
TTCTCGAGGTCAAGGGCGGAAAGTGGACCTTGCTGGACACCCGCATGCCTGAATAA

Upstream 100 bases:

>100_bases
ATCCGCGCCTGCAGGAAGCCTATCTCGGCGAGGGCAGCCACGCCCAAGCCCAACACGCGTAAACGCGCTGACGATCATCT
TTTCAGAAGGAGCCCGGTTC

Downstream 100 bases:

>100_bases
GGCATACGGGCGGGACCGGCAGCGCCCTCTCTCCCCTTGCTGCCGGTCCCACTTCTTTTTCCGCGTACTCAGGAAGCGAA
CATGACGGGCACCGCCCTCA

Product: ABC transporter periplasmic protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKKSLVFGATLLAAVSAAAMARAADDELVLGYMMAKSGPYVSLANTNEIAVDLAVEEINAKGGINGKKIKVVKFDTAGDP
KQATTALRRFAEDDSALAVIGPFSSSEVRTTFPVGERSGIAQMSMASSAPGLTKGFSYGFRNTTDEGIVLDQVMTSIKDK
KLPAASGAAAYATDDVVSKSIGTLVVPKLFEKYSVPMKGSVDFQLAAFDLSPQVSKLKEMAPDIVGLGSPPEGAVNLAKE
LKRQGVSTRVIGGTTIADPELPKRMDGAGENLTIGTTFFPEVNDKTKAFAAEFGKRTKAAGLPRTEPNQMDAAVYDIVYL
YAEAMKRAAVTGDKAKLVAERTGVRDALAKMKDFEALEGKISFRDGDAVKPVYILEVKGGKWTLLDTRMPE

Sequences:

>Translated_391_residues
MKKSLVFGATLLAAVSAAAMARAADDELVLGYMMAKSGPYVSLANTNEIAVDLAVEEINAKGGINGKKIKVVKFDTAGDP
KQATTALRRFAEDDSALAVIGPFSSSEVRTTFPVGERSGIAQMSMASSAPGLTKGFSYGFRNTTDEGIVLDQVMTSIKDK
KLPAASGAAAYATDDVVSKSIGTLVVPKLFEKYSVPMKGSVDFQLAAFDLSPQVSKLKEMAPDIVGLGSPPEGAVNLAKE
LKRQGVSTRVIGGTTIADPELPKRMDGAGENLTIGTTFFPEVNDKTKAFAAEFGKRTKAAGLPRTEPNQMDAAVYDIVYL
YAEAMKRAAVTGDKAKLVAERTGVRDALAKMKDFEALEGKISFRDGDAVKPVYILEVKGGKWTLLDTRMPE
>Mature_391_residues
MKKSLVFGATLLAAVSAAAMARAADDELVLGYMMAKSGPYVSLANTNEIAVDLAVEEINAKGGINGKKIKVVKFDTAGDP
KQATTALRRFAEDDSALAVIGPFSSSEVRTTFPVGERSGIAQMSMASSAPGLTKGFSYGFRNTTDEGIVLDQVMTSIKDK
KLPAASGAAAYATDDVVSKSIGTLVVPKLFEKYSVPMKGSVDFQLAAFDLSPQVSKLKEMAPDIVGLGSPPEGAVNLAKE
LKRQGVSTRVIGGTTIADPELPKRMDGAGENLTIGTTFFPEVNDKTKAFAAEFGKRTKAAGLPRTEPNQMDAAVYDIVYL
YAEAMKRAAVTGDKAKLVAERTGVRDALAKMKDFEALEGKISFRDGDAVKPVYILEVKGGKWTLLDTRMPE

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=279, Percent_Identity=26.1648745519713, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789867, Length=140, Percent_Identity=32.8571428571429, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41483; Mature: 41483

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLVFGATLLAAVSAAAMARAADDELVLGYMMAKSGPYVSLANTNEIAVDLAVEEINA
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCEEEEEEEEECCC
KGGINGKKIKVVKFDTAGDPKQATTALRRFAEDDSALAVIGPFSSSEVRTTFPVGERSGI
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCCCC
AQMSMASSAPGLTKGFSYGFRNTTDEGIVLDQVMTSIKDKKLPAASGAAAYATDDVVSKS
HHHHHHCCCCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IGTLVVPKLFEKYSVPMKGSVDFQLAAFDLSPQVSKLKEMAPDIVGLGSPPEGAVNLAKE
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCHHEECCCCCHHHHHHHHH
LKRQGVSTRVIGGTTIADPELPKRMDGAGENLTIGTTFFPEVNDKTKAFAAEFGKRTKAA
HHHCCCCEEEECCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHC
GLPRTEPNQMDAAVYDIVYLYAEAMKRAAVTGDKAKLVAERTGVRDALAKMKDFEALEGK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCE
ISFRDGDAVKPVYILEVKGGKWTLLDTRMPE
EEECCCCCCCCEEEEEECCCEEEEECCCCCC
>Mature Secondary Structure
MKKSLVFGATLLAAVSAAAMARAADDELVLGYMMAKSGPYVSLANTNEIAVDLAVEEINA
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCEEEEEEEEECCC
KGGINGKKIKVVKFDTAGDPKQATTALRRFAEDDSALAVIGPFSSSEVRTTFPVGERSGI
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCCCC
AQMSMASSAPGLTKGFSYGFRNTTDEGIVLDQVMTSIKDKKLPAASGAAAYATDDVVSKS
HHHHHHCCCCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IGTLVVPKLFEKYSVPMKGSVDFQLAAFDLSPQVSKLKEMAPDIVGLGSPPEGAVNLAKE
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCHHEECCCCCHHHHHHHHH
LKRQGVSTRVIGGTTIADPELPKRMDGAGENLTIGTTFFPEVNDKTKAFAAEFGKRTKAA
HHHCCCCEEEECCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHC
GLPRTEPNQMDAAVYDIVYLYAEAMKRAAVTGDKAKLVAERTGVRDALAKMKDFEALEGK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCE
ISFRDGDAVKPVYILEVKGGKWTLLDTRMPE
EEECCCCCCCCEEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA