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The map label for this gene is iclR [H]

Identifier: 158422089

GI number: 158422089

Start: 520663

End: 521514

Strand: Direct

Name: iclR [H]

Synonym: AZC_0465

Alternate gene names: 158422089

Gene position: 520663-521514 (Clockwise)

Preceding gene: 158422087

Following gene: 158422090

Centisome position: 9.7

GC content: 71.01

Gene sequence:

>852_bases
ATGCCCGATACGGATCTTGCCCCCCGCCGTCGCGGACGTCCGGCCTCCACGTCGCCGGACGGAGGCGCCGAGGTCCAGTC
GCTGGACCGGGCGATCCATCTCCTGCGCATCCTCGCCGAAGCCGACGGCATGAGCCTCACGGACGTGGCCCGCCGCGCGG
AACTGCCGGCCTCCACGACCCATCGCCTGCTGGCGACGCTCCAGAAGCGCCAACTGGTCAATCATGACGCGGAGACGGGC
CTGTGGACCGTCGGCCTCGGCCTCTTCCGCATCGGCGCGGCCTATCTGCGCATCCGCAAGCTGCCGGACATCGGCCGCCC
GGTCATCCGCCGCCTGCTGCATGATGCGGACGAAACGGTGAACCTCTCCATGTTCGACGGCGCCGAACTGGTGGTGGTCG
GGCAGGCGGAAGGCCATGCCAGCGTGCGCGCCTTCTTCCGCCTCGGCCAGCGGCTCCCGCTCCACGCCACCGCCGCCGGC
AAGAGCATCCTCGCCGCCGCCGGCCCCGCCTTCCGCGGCGCGCTGCTGGCGCAGGTGCGCTACGAGCCGCTGGGCGTGCG
CACCCACCGCACCGAGCGCGCCCTCATGGACGACATCGCCCAGATCATCGAGCGCGGCTATGCGGTGGACAATGAGGAGC
ACACCACCGGCATGCGGGCCGTGGCGGCGGCCATCTACAACGAATGGCGCGAACCCATCGGGGCGGTGTCCGTCTCCGGT
CCTTCCGGCCGCATGACGGACGATCGCGTCGCCCGGCTCGGACGCCTCATCACCGCCGCCGCCGACCAGATGACGCGCCT
GTATTCCGGCGCCGACGAGACTTTGCCGCCGGATTTCGCGACATATCGCTGA

Upstream 100 bases:

>100_bases
TCCGGCTGTGCGAAACGGCCGCGCGCCCTTGCAAAATCAGCGCTGGCGGCAGAAGTTGAGCCCCGTCGGCGGCACGCCGG
CCGCTCGCCTGGAGCCCTGA

Downstream 100 bases:

>100_bases
CTATGTGCTCAACGGTCATTGATGCTCTCGGCCGTTACATCGTCTTTTCCTGTGGGCACGGTTCAATCCCACCGTTCTTT
TGACGATTGGCAGTAAAGGG

Product: regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MPDTDLAPRRRGRPASTSPDGGAEVQSLDRAIHLLRILAEADGMSLTDVARRAELPASTTHRLLATLQKRQLVNHDAETG
LWTVGLGLFRIGAAYLRIRKLPDIGRPVIRRLLHDADETVNLSMFDGAELVVVGQAEGHASVRAFFRLGQRLPLHATAAG
KSILAAAGPAFRGALLAQVRYEPLGVRTHRTERALMDDIAQIIERGYAVDNEEHTTGMRAVAAAIYNEWREPIGAVSVSG
PSGRMTDDRVARLGRLITAAADQMTRLYSGADETLPPDFATYR

Sequences:

>Translated_283_residues
MPDTDLAPRRRGRPASTSPDGGAEVQSLDRAIHLLRILAEADGMSLTDVARRAELPASTTHRLLATLQKRQLVNHDAETG
LWTVGLGLFRIGAAYLRIRKLPDIGRPVIRRLLHDADETVNLSMFDGAELVVVGQAEGHASVRAFFRLGQRLPLHATAAG
KSILAAAGPAFRGALLAQVRYEPLGVRTHRTERALMDDIAQIIERGYAVDNEEHTTGMRAVAAAIYNEWREPIGAVSVSG
PSGRMTDDRVARLGRLITAAADQMTRLYSGADETLPPDFATYR
>Mature_282_residues
PDTDLAPRRRGRPASTSPDGGAEVQSLDRAIHLLRILAEADGMSLTDVARRAELPASTTHRLLATLQKRQLVNHDAETGL
WTVGLGLFRIGAAYLRIRKLPDIGRPVIRRLLHDADETVNLSMFDGAELVVVGQAEGHASVRAFFRLGQRLPLHATAAGK
SILAAAGPAFRGALLAQVRYEPLGVRTHRTERALMDDIAQIIERGYAVDNEEHTTGMRAVAAAIYNEWREPIGAVSVSGP
SGRMTDDRVARLGRLITAAADQMTRLYSGADETLPPDFATYR

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=270, Percent_Identity=39.2592592592593, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786715, Length=250, Percent_Identity=38.8, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=32.258064516129, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788131, Length=247, Percent_Identity=27.5303643724696, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789999, Length=244, Percent_Identity=28.2786885245902, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788581, Length=248, Percent_Identity=24.1935483870968, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30799; Mature: 30667

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTDLAPRRRGRPASTSPDGGAEVQSLDRAIHLLRILAEADGMSLTDVARRAELPASTT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHH
HRLLATLQKRQLVNHDAETGLWTVGLGLFRIGAAYLRIRKLPDIGRPVIRRLLHDADETV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCE
NLSMFDGAELVVVGQAEGHASVRAFFRLGQRLPLHATAAGKSILAAAGPAFRGALLAQVR
EEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCEEHHCCCHHHHHCCHHHHHHHHHHHH
YEPLGVRTHRTERALMDDIAQIIERGYAVDNEEHTTGMRAVAAAIYNEWREPIGAVSVSG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECC
PSGRMTDDRVARLGRLITAAADQMTRLYSGADETLPPDFATYR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDTDLAPRRRGRPASTSPDGGAEVQSLDRAIHLLRILAEADGMSLTDVARRAELPASTT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHH
HRLLATLQKRQLVNHDAETGLWTVGLGLFRIGAAYLRIRKLPDIGRPVIRRLLHDADETV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCE
NLSMFDGAELVVVGQAEGHASVRAFFRLGQRLPLHATAAGKSILAAAGPAFRGALLAQVR
EEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCEEHHCCCHHHHHCCHHHHHHHHHHHH
YEPLGVRTHRTERALMDDIAQIIERGYAVDNEEHTTGMRAVAAAIYNEWREPIGAVSVSG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECC
PSGRMTDDRVARLGRLITAAADQMTRLYSGADETLPPDFATYR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]