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The map label for this gene is lig [H]

Identifier: 158422017

GI number: 158422017

Start: 447259

End: 448917

Strand: Reverse

Name: lig [H]

Synonym: AZC_0393

Alternate gene names: 158422017

Gene position: 448917-447259 (Counterclockwise)

Preceding gene: 158422018

Following gene: 158422015

Centisome position: 8.36

GC content: 68.78

Gene sequence:

>1659_bases
ATGAACCGCTTCGCCGAACTTCTGGACCGCCTCTCCTACGAGCCCCGCCGCAACGCCAAGATCCGGCTGATGAGCGAGTA
TTTCCGCTCGACGCCGGACCCAGAGCGGGGGGTGGCGCTCGCCGCCCTGACCGGCAAGCTCTCCTTCACCAATGCCAAGC
CCGGCCTTATCCGCGCCCTCATCGCCGAGCGCACGGACCCGGTGCTGTTCGAACTCTCCTACGACTATGTGGGCGACCTC
TCCGAGACCGTCGCCCTCATGTGGCCCGCGCCGAAGGGCCTGCCGGAGATGGGTGGCAACCACGCGCCCTTCCTCTCCGA
GATCGTCGAGGGGCTGGAAACGCTGCCCAAGCGCGACCTGCCCCGCCAGCTCGCCCTCTGGCTCGACGGGCTGGACGAAA
CCGGCCGCTGGGCGCTCTTGAAGCTGATTACCGGCGCATTGCGCGTGGGTGCTTCCGCGCGCCTCGCCAAGACCGCCGTG
GCGGCCCTGGGCGACCTGCCGCCGGACGAGGTGGAACTGGTCTGGCCCGGCCTTTCCATGCCCTATCGCGAGCTTTTCGC
CTGGGCCGAAGGCAAGGGGGAGCGGCCGCAGACCACGGACCCGGTGCCCTTCCGCCCGGTGATGCTCGCCCATGCCATCG
AGGAGACGGATTTTTCCGCCCTCGATCCCGCGAGCTTCGCCGCCGAATGGAAATGGGACGGCATCCGCGTGCAGGCGGTC
TGCGCTCGCAGCAGCGACGGCACGCGGGCGGCACGCCTCTACAGCCGCACGGGCGAAGACATTTCCGGCGCCTTCCCTGA
CCTCGTGGAGGCACTCGATTTCGACGGCGCGCTCGACGGCGAACTGCTCATCGTGCGCGAGAAAAGGGTCGAGACCTTTA
ACGTTCTTCAGCAGCGGCTCAACCGCAAGGCCGTCACCACAAAGATGCTGGCGGAATTCCCCGCCCATATCCGGGCCTAT
GACCTGCTCGCCGTCGCTGGCGAAGACCTGCGACCACTGCCCTTCCGCGACCGGCGGGCGCGACTGGAGGCGCTGATCGC
GCGGCTCGAGACCCAAGGCCACACGCGGCTTGACCTCTCCCCCCTCGTGCCGTTCGCGGATTGGGAGGGCCTCGCCGCCG
CCCGCCGCGATCCGGCCTCGGCCGGAGCCGGCCTTGATGCGGAGGCGGTGGAAGGGGTGATGCTGAAGGGCTGGGACACG
GCCTATCTCCCCGGCCGCCCCAAGGGGCCGTGGTGGAAATGGAAGCGCGATCCCCACACCGTGGATGCCGTGCTCATGTA
TGCCCAGCGCGGCCACGGCAAGCGCTCCTCCTTCTATTCCGATTACACCTTCGGCGTCTGGACGCAGGGCGAGGAGGGCG
ATGTGCTGGTGCCGGTCGGCAAGGCCTATTTCGGCTTTACCGATCAGGAGCTCATCGAGATCGACAAGTTCGTGCGCCGC
GCCACCATCAACCGCTTCGGCCCGGTGCGCGAGGTGCTGCACGAGCCGGACGAGGGACTGGTGCTGGAAGTCGCCTTCGA
GGGCCTCGCCCGCTCCACCCGCCACCGCTCGGGCGTCGCCATGCGCTTCCCTCGCATCGCCCGCCTGCGCTGGGACAAGC
CGCCCCGCGAGGCCGACCGGCTGGAGACGCTGGAAGCGCTGCTGCGGCAGGAGGAGTAA

Upstream 100 bases:

>100_bases
TGGGCTATGCGGAGGAGGACGAGGCGGCGGAGGGCGAGGCCGTGGCGATGTCCGGTCCCGAGGCGCCGCCGCAGGATGGC
GCACCGGAGGAGACGGCGCC

Downstream 100 bases:

>100_bases
CCTCTCCCCCGGCGCCTACTCGACCGGCACGCTGCGGCGCGTGGCAAAGGACAGGCGGGTCTTGATGCGCGTCGCCTGCT
GCGACACGGCAATCGCCCGA

Product: ATP dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MNRFAELLDRLSYEPRRNAKIRLMSEYFRSTPDPERGVALAALTGKLSFTNAKPGLIRALIAERTDPVLFELSYDYVGDL
SETVALMWPAPKGLPEMGGNHAPFLSEIVEGLETLPKRDLPRQLALWLDGLDETGRWALLKLITGALRVGASARLAKTAV
AALGDLPPDEVELVWPGLSMPYRELFAWAEGKGERPQTTDPVPFRPVMLAHAIEETDFSALDPASFAAEWKWDGIRVQAV
CARSSDGTRAARLYSRTGEDISGAFPDLVEALDFDGALDGELLIVREKRVETFNVLQQRLNRKAVTTKMLAEFPAHIRAY
DLLAVAGEDLRPLPFRDRRARLEALIARLETQGHTRLDLSPLVPFADWEGLAAARRDPASAGAGLDAEAVEGVMLKGWDT
AYLPGRPKGPWWKWKRDPHTVDAVLMYAQRGHGKRSSFYSDYTFGVWTQGEEGDVLVPVGKAYFGFTDQELIEIDKFVRR
ATINRFGPVREVLHEPDEGLVLEVAFEGLARSTRHRSGVAMRFPRIARLRWDKPPREADRLETLEALLRQEE

Sequences:

>Translated_552_residues
MNRFAELLDRLSYEPRRNAKIRLMSEYFRSTPDPERGVALAALTGKLSFTNAKPGLIRALIAERTDPVLFELSYDYVGDL
SETVALMWPAPKGLPEMGGNHAPFLSEIVEGLETLPKRDLPRQLALWLDGLDETGRWALLKLITGALRVGASARLAKTAV
AALGDLPPDEVELVWPGLSMPYRELFAWAEGKGERPQTTDPVPFRPVMLAHAIEETDFSALDPASFAAEWKWDGIRVQAV
CARSSDGTRAARLYSRTGEDISGAFPDLVEALDFDGALDGELLIVREKRVETFNVLQQRLNRKAVTTKMLAEFPAHIRAY
DLLAVAGEDLRPLPFRDRRARLEALIARLETQGHTRLDLSPLVPFADWEGLAAARRDPASAGAGLDAEAVEGVMLKGWDT
AYLPGRPKGPWWKWKRDPHTVDAVLMYAQRGHGKRSSFYSDYTFGVWTQGEEGDVLVPVGKAYFGFTDQELIEIDKFVRR
ATINRFGPVREVLHEPDEGLVLEVAFEGLARSTRHRSGVAMRFPRIARLRWDKPPREADRLETLEALLRQEE
>Mature_552_residues
MNRFAELLDRLSYEPRRNAKIRLMSEYFRSTPDPERGVALAALTGKLSFTNAKPGLIRALIAERTDPVLFELSYDYVGDL
SETVALMWPAPKGLPEMGGNHAPFLSEIVEGLETLPKRDLPRQLALWLDGLDETGRWALLKLITGALRVGASARLAKTAV
AALGDLPPDEVELVWPGLSMPYRELFAWAEGKGERPQTTDPVPFRPVMLAHAIEETDFSALDPASFAAEWKWDGIRVQAV
CARSSDGTRAARLYSRTGEDISGAFPDLVEALDFDGALDGELLIVREKRVETFNVLQQRLNRKAVTTKMLAEFPAHIRAY
DLLAVAGEDLRPLPFRDRRARLEALIARLETQGHTRLDLSPLVPFADWEGLAAARRDPASAGAGLDAEAVEGVMLKGWDT
AYLPGRPKGPWWKWKRDPHTVDAVLMYAQRGHGKRSSFYSDYTFGVWTQGEEGDVLVPVGKAYFGFTDQELIEIDKFVRR
ATINRFGPVREVLHEPDEGLVLEVAFEGLARSTRHRSGVAMRFPRIARLRWDKPPREADRLETLEALLRQEE

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=388, Percent_Identity=26.2886597938144, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71985269, Length=394, Percent_Identity=26.6497461928934, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI85815841, Length=543, Percent_Identity=26.3351749539595, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 61816; Mature: 61816

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFAELLDRLSYEPRRNAKIRLMSEYFRSTPDPERGVALAALTGKLSFTNAKPGLIRAL
CCHHHHHHHHHCCCCCCCCCEEEHHHHHHCCCCHHHCEEEEEEECEEEECCCCCHHHHHH
IAERTDPVLFELSYDYVGDLSETVALMWPAPKGLPEMGGNHAPFLSEIVEGLETLPKRDL
HHCCCCCEEEEECCHHHCCCHHHEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCC
PRQLALWLDGLDETGRWALLKLITGALRVGASARLAKTAVAALGDLPPDEVELVWPGLSM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCC
PYRELFAWAEGKGERPQTTDPVPFRPVMLAHAIEETDFSALDPASFAAEWKWDGIRVQAV
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEE
CARSSDGTRAARLYSRTGEDISGAFPDLVEALDFDGALDGELLIVREKRVETFNVLQQRL
EECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHH
NRKAVTTKMLAEFPAHIRAYDLLAVAGEDLRPLPFRDRRARLEALIARLETQGHTRLDLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCC
PLVPFADWEGLAAARRDPASAGAGLDAEAVEGVMLKGWDTAYLPGRPKGPWWKWKRDPHT
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHCCEEEECCCEEECCCCCCCCCCCCCCCCCH
VDAVLMYAQRGHGKRSSFYSDYTFGVWTQGEEGDVLVPVGKAYFGFTDQELIEIDKFVRR
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHCCCCHHHHHHHHHHHHH
ATINRFGPVREVLHEPDEGLVLEVAFEGLARSTRHRSGVAMRFPRIARLRWDKPPREADR
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCEECCCHHHHHCCCCCCCHHHH
LETLEALLRQEE
HHHHHHHHHCCC
>Mature Secondary Structure
MNRFAELLDRLSYEPRRNAKIRLMSEYFRSTPDPERGVALAALTGKLSFTNAKPGLIRAL
CCHHHHHHHHHCCCCCCCCCEEEHHHHHHCCCCHHHCEEEEEEECEEEECCCCCHHHHHH
IAERTDPVLFELSYDYVGDLSETVALMWPAPKGLPEMGGNHAPFLSEIVEGLETLPKRDL
HHCCCCCEEEEECCHHHCCCHHHEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCC
PRQLALWLDGLDETGRWALLKLITGALRVGASARLAKTAVAALGDLPPDEVELVWPGLSM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCC
PYRELFAWAEGKGERPQTTDPVPFRPVMLAHAIEETDFSALDPASFAAEWKWDGIRVQAV
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEE
CARSSDGTRAARLYSRTGEDISGAFPDLVEALDFDGALDGELLIVREKRVETFNVLQQRL
EECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHH
NRKAVTTKMLAEFPAHIRAYDLLAVAGEDLRPLPFRDRRARLEALIARLETQGHTRLDLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCC
PLVPFADWEGLAAARRDPASAGAGLDAEAVEGVMLKGWDTAYLPGRPKGPWWKWKRDPHT
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHCCEEEECCCEEECCCCCCCCCCCCCCCCCH
VDAVLMYAQRGHGKRSSFYSDYTFGVWTQGEEGDVLVPVGKAYFGFTDQELIEIDKFVRR
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHCCCCHHHHHHHHHHHHH
ATINRFGPVREVLHEPDEGLVLEVAFEGLARSTRHRSGVAMRFPRIARLRWDKPPREADR
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCEECCCHHHHHCCCCCCCHHHH
LETLEALLRQEE
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA