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The map label for this gene is gluQ [H]

Identifier: 158421996

GI number: 158421996

Start: 426621

End: 427529

Strand: Direct

Name: gluQ [H]

Synonym: AZC_0372

Alternate gene names: 158421996

Gene position: 426621-427529 (Clockwise)

Preceding gene: 158421992

Following gene: 158421999

Centisome position: 7.94

GC content: 70.74

Gene sequence:

>909_bases
ATGACCATCGTCTGCCGCTTCGCCCCAAGCCCCAACGGCTTCCTGCATCTCGGCCACGCGCATTCGGCTCTGGTGAACCA
TGCCGCCGCCACGCGCGCCGCCGGCCGCTTCCTGTTGCGCATGGAAGACATCGACACCACCCGATGTCGCCCGGAATACG
AGCAGGGCATCATCGACGATCTGGACTGGCTCGGCATCCTCCCGCCCGAGCCGCCCCGCCACCAGAGCCGGCACTTCGAC
GATTACGCCGCAGCCCTCGCGCGCCTTGAGGAGATGGGGCTGCTCTACGCTTCTTTCGAGACCCGCGCGGAGATCGCCCG
CGCCGTCCTCGCCCGCGAGGCGGCGGGCCGGTCCTGGCCGCGTGATCCCGACGGTGTGCCGCTCTATCCCTTTTCCCGCG
AGAGCATGACGGAGGCGGCCCGCGCGCGCCGCAAGGCCGAGGGCGAGCCTTATGTGCTGCGCCTCGACATGGGGCAGGCC
TATGCGCTGGCGGGTGCCGGCCTCTCCTGGCACGAGGCGCCTGGCGATCCGCTGGCGGAGCCCCTGCCGGTTCAGGCCGA
TCCGGCAGCCTGGGGCGATGTGGTGCTCGCCCGCAAGGACGTGCCGGCGAGCTATCATCTGGCGGTGGTGATGGATGACG
ACCTGCAGGGCATCACGCATGTGATCCGCGGCCGCGACCTTTACGCCGCCACCGCCATCCATCGCCTGCTCCAGGCGCTG
CTCGGCCTGCCGGCGCCCATCTATCATCACCACGCGCTGGTGCTGGACCGCGACGGGCGCAAGCTGTCTAAAAGCACGGG
GGCGGCGAGCCTGTCGTCCCTGCGCGCGCTCGGCACCACGCCGGACGAGGTGCGCGCGCTCATCGCACACGGGCTGGAGC
CGGCGTCTCAGCCCACCCCGAGCACGTAG

Upstream 100 bases:

>100_bases
TCGCTTGGTCGCTCAGGTGAACCTCGCCCATCATGCCGCGCCTCCTTGCCCTTTTGCCCTCTCGCGCCGGAGCGGCGGTC
ATGCGATGACATGGCCTGCC

Downstream 100 bases:

>100_bases
AGCCAGAAGGTGATGGTGAAGAGGCCGAGGGCCGTGGACAGCGAGACGGAGCTGGAACTGGTGCCGACGCCGACGCCATA
ACGCTGGGCCAGCAGATAGG

Product: glutamyl-tRNA synthetase

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTIVCRFAPSPNGFLHLGHAHSALVNHAAATRAAGRFLLRMEDIDTTRCRPEYEQGIIDDLDWLGILPPEPPRHQSRHFD
DYAAALARLEEMGLLYASFETRAEIARAVLAREAAGRSWPRDPDGVPLYPFSRESMTEAARARRKAEGEPYVLRLDMGQA
YALAGAGLSWHEAPGDPLAEPLPVQADPAAWGDVVLARKDVPASYHLAVVMDDDLQGITHVIRGRDLYAATAIHRLLQAL
LGLPAPIYHHHALVLDRDGRKLSKSTGAASLSSLRALGTTPDEVRALIAHGLEPASQPTPST

Sequences:

>Translated_302_residues
MTIVCRFAPSPNGFLHLGHAHSALVNHAAATRAAGRFLLRMEDIDTTRCRPEYEQGIIDDLDWLGILPPEPPRHQSRHFD
DYAAALARLEEMGLLYASFETRAEIARAVLAREAAGRSWPRDPDGVPLYPFSRESMTEAARARRKAEGEPYVLRLDMGQA
YALAGAGLSWHEAPGDPLAEPLPVQADPAAWGDVVLARKDVPASYHLAVVMDDDLQGITHVIRGRDLYAATAIHRLLQAL
LGLPAPIYHHHALVLDRDGRKLSKSTGAASLSSLRALGTTPDEVRALIAHGLEPASQPTPST
>Mature_301_residues
TIVCRFAPSPNGFLHLGHAHSALVNHAAATRAAGRFLLRMEDIDTTRCRPEYEQGIIDDLDWLGILPPEPPRHQSRHFDD
YAAALARLEEMGLLYASFETRAEIARAVLAREAAGRSWPRDPDGVPLYPFSRESMTEAARARRKAEGEPYVLRLDMGQAY
ALAGAGLSWHEAPGDPLAEPLPVQADPAAWGDVVLARKDVPASYHLAVVMDDDLQGITHVIRGRDLYAATAIHRLLQALL
GLPAPIYHHHALVLDRDGRKLSKSTGAASLSSLRALGTTPDEVRALIAHGLEPASQPTPST

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=301, Percent_Identity=34.21926910299, Blast_Score=137, Evalue=8e-33,
Organism=Escherichia coli, GI1788743, Length=282, Percent_Identity=31.5602836879433, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI226510925, Length=268, Percent_Identity=32.089552238806, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786895, Length=96, Percent_Identity=36.4583333333333, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=298, Percent_Identity=28.1879194630872, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324540, Length=284, Percent_Identity=30.9859154929577, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321192, Length=64, Percent_Identity=40.625, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24665321, Length=287, Percent_Identity=34.1463414634146, Blast_Score=131, Evalue=4e-31,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 32892; Mature: 32760

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVCRFAPSPNGFLHLGHAHSALVNHAAATRAAGRFLLRMEDIDTTRCRPEYEQGIIDD
CEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHCCCCC
LDWLGILPPEPPRHQSRHFDDYAAALARLEEMGLLYASFETRAEIARAVLAREAAGRSWP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RDPDGVPLYPFSRESMTEAARARRKAEGEPYVLRLDMGQAYALAGAGLSWHEAPGDPLAE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCC
PLPVQADPAAWGDVVLARKDVPASYHLAVVMDDDLQGITHVIRGRDLYAATAIHRLLQAL
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHH
LGLPAPIYHHHALVLDRDGRKLSKSTGAASLSSLRALGTTPDEVRALIAHGLEPASQPTP
HCCCCCHHHCEEEEEECCCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
ST
CC
>Mature Secondary Structure 
TIVCRFAPSPNGFLHLGHAHSALVNHAAATRAAGRFLLRMEDIDTTRCRPEYEQGIIDD
EEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHCCCCC
LDWLGILPPEPPRHQSRHFDDYAAALARLEEMGLLYASFETRAEIARAVLAREAAGRSWP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RDPDGVPLYPFSRESMTEAARARRKAEGEPYVLRLDMGQAYALAGAGLSWHEAPGDPLAE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCC
PLPVQADPAAWGDVVLARKDVPASYHLAVVMDDDLQGITHVIRGRDLYAATAIHRLLQAL
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHH
LGLPAPIYHHHALVLDRDGRKLSKSTGAASLSSLRALGTTPDEVRALIAHGLEPASQPTP
HCCCCCHHHCEEEEEECCCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
ST
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA