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The map label for this gene is cysP [H]

Identifier: 158421926

GI number: 158421926

Start: 351913

End: 352953

Strand: Direct

Name: cysP [H]

Synonym: AZC_0302

Alternate gene names: 158421926

Gene position: 351913-352953 (Clockwise)

Preceding gene: 158421925

Following gene: 158421927

Centisome position: 6.55

GC content: 64.27

Gene sequence:

>1041_bases
ATGCTGAGCCGCCGTTCCGTCCTGTCCCTCACGCTCGGCGGCCTTGTCGCCAGCGCCGGCATGGGCAGCCTCTCCGCCGC
GCTGGCGCAGCAGATCAAGTACAGCGGCCCGAACACCCTGCTGAACGTCTCCTATGACATAGCCCGCGAGTTGTTCACGG
CGGAGAACACGGAGTTCGTGCCCTTCTGGAAGCAGCAGACGGGGCAGGACATCACGGTCAACCAGAGCCACGCCGGCTCC
TCCCGCCAGGCCCGCGCCATCATCGAGGGGCTTGAGGCCGACGTCGTGACCTTCAATCAGGTGCTGGACGTGCAGGCGCT
GGTGAAGGCGGGCTTCGTGGCCAAGGACTGGCAGGCGAAATTCCCGAACAATGCCTCGCCTTATTATTCCTTCCCGGCCT
TCCTCGTGCGCGCCGGCAATCCCAAGAACATCAAGGACTGGAACGACCTCGTCCGCGACGACGTGAAGGTCATCTTCCCG
AACCCCAAGACCTCGGGGAATGCGCGTTACACCTATCTCGCCGCCTACGCCTATGCCCTGGAGCAGAACAAGGGTGACAC
CGCCAAGGCCGACGCCTTCGTGAAGAAGCTGCTCGCCAACGTGCCGGTGTTCGACACGGGCGGCCGTGGGGCCACCACCA
CCTTCGTGGAACGCGAGACGGGCGACGTGCTGGTGAGCTTCGAGGCCGAGACCCGCGCCATTGCGGACGTCTACAAGGAC
AAGAAGCTCTCCACCGTGGTGCCGTCCGTGAGCGTGCTGGCCGAGTTTCCGGTGGCCGTGGTGGACAAGGTGGTGGACAA
GCGCGGCTCGCGCGTCGCCGCCCAGCAGTATCTGGAGTTCCTATATTCCCCCGCTGGCCAGACGGTGGCTGCCAAGGCTT
ATCACCGTGTGGTGGACAAGACCGTGGAGGAGAAGTTCAAGGATCAGTTCCCGCCGGTGCGCCTCGTGAAGGTGGAAGAC
GTGTTCGGCGGCTGGGACAAGGCCAATGCCGATCACTTCGTCTCCGGTGGGAAACTCGATCAGGTCTTCGGCGCCCGCTG
A

Upstream 100 bases:

>100_bases
CTTTCATCCATATTCCCAATGGGAAATGTCTGTGCTACGCATTTCTCAAGCGTGAATTAAAAATCCACGCAAATTTAATG
AGAAATGCCAGGAGCCTCCG

Downstream 100 bases:

>100_bases
GCGGCGGTCCCTTAAGATTTCCTTCAGCCGTCCCTCGCCTTTCGCAAGAGGACCGTCATGCCCGGGCTCGTCCTGGGCAT
CCACGCGCGGCTGGCGAGAA

Product: thiosulphate-binding protein

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MLSRRSVLSLTLGGLVASAGMGSLSAALAQQIKYSGPNTLLNVSYDIARELFTAENTEFVPFWKQQTGQDITVNQSHAGS
SRQARAIIEGLEADVVTFNQVLDVQALVKAGFVAKDWQAKFPNNASPYYSFPAFLVRAGNPKNIKDWNDLVRDDVKVIFP
NPKTSGNARYTYLAAYAYALEQNKGDTAKADAFVKKLLANVPVFDTGGRGATTTFVERETGDVLVSFEAETRAIADVYKD
KKLSTVVPSVSVLAEFPVAVVDKVVDKRGSRVAAQQYLEFLYSPAGQTVAAKAYHRVVDKTVEEKFKDQFPPVRLVKVED
VFGGWDKANADHFVSGGKLDQVFGAR

Sequences:

>Translated_346_residues
MLSRRSVLSLTLGGLVASAGMGSLSAALAQQIKYSGPNTLLNVSYDIARELFTAENTEFVPFWKQQTGQDITVNQSHAGS
SRQARAIIEGLEADVVTFNQVLDVQALVKAGFVAKDWQAKFPNNASPYYSFPAFLVRAGNPKNIKDWNDLVRDDVKVIFP
NPKTSGNARYTYLAAYAYALEQNKGDTAKADAFVKKLLANVPVFDTGGRGATTTFVERETGDVLVSFEAETRAIADVYKD
KKLSTVVPSVSVLAEFPVAVVDKVVDKRGSRVAAQQYLEFLYSPAGQTVAAKAYHRVVDKTVEEKFKDQFPPVRLVKVED
VFGGWDKANADHFVSGGKLDQVFGAR
>Mature_346_residues
MLSRRSVLSLTLGGLVASAGMGSLSAALAQQIKYSGPNTLLNVSYDIARELFTAENTEFVPFWKQQTGQDITVNQSHAGS
SRQARAIIEGLEADVVTFNQVLDVQALVKAGFVAKDWQAKFPNNASPYYSFPAFLVRAGNPKNIKDWNDLVRDDVKVIFP
NPKTSGNARYTYLAAYAYALEQNKGDTAKADAFVKKLLANVPVFDTGGRGATTTFVERETGDVLVSFEAETRAIADVYKD
KKLSTVVPSVSVLAEFPVAVVDKVVDKRGSRVAAQQYLEFLYSPAGQTVAAKAYHRVVDKTVEEKFKDQFPPVRLVKVED
VFGGWDKANADHFVSGGKLDQVFGAR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=308, Percent_Identity=55.8441558441558, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1790351, Length=303, Percent_Identity=50.4950495049505, Blast_Score=322, Evalue=3e-89,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37805; Mature: 37805

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRRSVLSLTLGGLVASAGMGSLSAALAQQIKYSGPNTLLNVSYDIARELFTAENTEFV
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCCCCC
PFWKQQTGQDITVNQSHAGSSRQARAIIEGLEADVVTFNQVLDVQALVKAGFVAKDWQAK
EEHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
FPNNASPYYSFPAFLVRAGNPKNIKDWNDLVRDDVKVIFPNPKTSGNARYTYLAAYAYAL
CCCCCCCCEECCCCEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCEEEHHHHHHHHH
EQNKGDTAKADAFVKKLLANVPVFDTGGRGATTTFVERETGDVLVSFEAETRAIADVYKD
HCCCCCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHC
KKLSTVVPSVSVLAEFPVAVVDKVVDKRGSRVAAQQYLEFLYSPAGQTVAAKAYHRVVDK
CHHHHHCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TVEEKFKDQFPPVRLVKVEDVFGGWDKANADHFVSGGKLDQVFGAR
HHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MLSRRSVLSLTLGGLVASAGMGSLSAALAQQIKYSGPNTLLNVSYDIARELFTAENTEFV
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCCCCC
PFWKQQTGQDITVNQSHAGSSRQARAIIEGLEADVVTFNQVLDVQALVKAGFVAKDWQAK
EEHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
FPNNASPYYSFPAFLVRAGNPKNIKDWNDLVRDDVKVIFPNPKTSGNARYTYLAAYAYAL
CCCCCCCCEECCCCEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCEEEHHHHHHHHH
EQNKGDTAKADAFVKKLLANVPVFDTGGRGATTTFVERETGDVLVSFEAETRAIADVYKD
HCCCCCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHC
KKLSTVVPSVSVLAEFPVAVVDKVVDKRGSRVAAQQYLEFLYSPAGQTVAAKAYHRVVDK
CHHHHHCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TVEEKFKDQFPPVRLVKVEDVFGGWDKANADHFVSGGKLDQVFGAR
HHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]