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The map label for this gene is lepA [H]

Identifier: 158421881

GI number: 158421881

Start: 300461

End: 302299

Strand: Reverse

Name: lepA [H]

Synonym: AZC_0257

Alternate gene names: 158421881

Gene position: 302299-300461 (Counterclockwise)

Preceding gene: 158421882

Following gene: 158421880

Centisome position: 5.63

GC content: 64.49

Gene sequence:

>1839_bases
GTGGCGCCTGACGCCCGATGCTATGCGGCGCCCATGACCGCGACCGCTCAGAAGAACATCCGCAACTTTTCCATCGTCGC
CCATATCGACCATGGGAAATCGACCCTCGCCGACCGCCTGATCCAGCTTACGGGCGGGCTCTCCGAGCGCGAGATGTCGG
ACCAGGTGCTCGACAATATGGACATCGAGCGCGAGCGCGGCATCACCATCAAGGCCCAGACCGTTCGCCTCTCCTACAAG
GCGCAGGACGGCGAGACCTATACGCTGAACCTCATCGACACCCCCGGGCACGTGGACTTCGCCTATGAGGTGAGCCGTTC
GCTCGCCGCCGTGGAAGGCTCGCTGCTGGTGGTGGACGCCTCGCAGGGGGTCGAGGCGCAGACGCTCGCCAACGTCTATC
AGGCCATCGACAACAATCACGACATCGTCCCGGTCCTCAACAAGATCGACCTGCCGGCGGCCGAGCCGGAGAAGGTGAAG
AGCCAGATCGAGGACGTGATCGGCCTCGATGCCTCCAACGCCATCGGCATCTCGGCCAAGACCGGCCTCAACATCGACCA
GGTGCTGGAAGCCATCGTCACCCGCCTGCCCGCCCCCGTGGGTGACCGCGAGGCGCCGCTGAAGGCGCTGCTGGTGGACA
GCTGGTACGACACCTATCTCGGCGTCGTCGTGCTGGTGCGCATCATCGACGGCGTGCTGAAGAAGGGCATGCGCATCAAG
ATGATGGGCGCGGATGCCGTCTATGACGTGGACCGCGTGGGCGTGTTCACGCCCAAGATGGTGGCCATGGAGAGCCTCGG
CCCCGGCGAGATCGGCTTCCTCAACGGCTCCATCAAGGAAGTGGCGGACACCCGCGTCGGCGACACGATCACCGACGACA
AGCGCCCAACGAGCGAGGCGCTGCCGGGCTTCAAGCCGGCCCAGCCGGTGGTGTTCTGCGGCCTCTTCCCCGTGGACGCG
GCGGACTTCGAGGACCTGCGCGCCGCCATGGGCAAGCTGCGCCTCAACGACGCCAGCTTCTCCTTCGAGATGGAAACCTC
GGCGGCGCTGGGCTTCGGCTTTCGCTGCGGCTTCCTCGGACTGCTGCATCTGGAGATCATCCAGGAGCGCCTGTCGCGCG
AGTTCAACCTCGACCTTATCGCCACCGCGCCGTCGGTCATCTACCAGATGGAGCTGACGGACGGCTCGACCATCGATCTG
CACAACCCGGCCGACATGCCGGACGTGGTGAAGATCGAGGAAATCCGCGAGCCCTGGATCCGCGCCACCATCATGACGCC
GGACGATTATCTCGGCGCGGTGCTGAAGCTCTGCCAGGACCGGCGCGGCAACCAGATCGAGCTGACCTATGTGGGCGCGC
GCGCCATGGTCACCTACGACCTGCCGCTGAACGAAGTGGTGTTCGACTTCTACGACAAGCTGAAGTCGATCTCGAAGGGC
TACGCCTCCTTCGACTACAACATCACCGACTATCGCCCCGGCGAACTGGTGAAGATGTCCATCCTCGTCAATGGCGAGCC
GGTGGACGCGCTGTCCATGCTGGTCCACCGCACGCGGGCGGAGTTCCGTGGCCGGGCCATGTGCGAGAAGCTGAAGGACC
TCATCCCGCAGCACCTGTTCAACATCCCGATCCAGGCCGCCATCGGCGCCAAGGTGATCGCGCGCGAGACCATCAAGGCC
CTGCGCAAGGACGTGACGGCGAAGTGCTACGGCGGCGACGTCTCGCGCAAGCGCAAGCTTCTGGACAAGCAGAAGGAAGG
CAAGAAGAAGATGCGCCAGTTCGGCAAGGTGGAGATTCCGCAGGAAGCCTTCATCGCCGCGCTGAAGATGGACGACTGA

Upstream 100 bases:

>100_bases
CTCTGACGGTTCCGGCGCGGCGGGCTCCGCCGCGCCACGCCCTGCGCCGGAATGGCACAGGTTTGACCGGCCGCGGGGCT
GCAGCGCCGGTTGCAGCCTT

Downstream 100 bases:

>100_bases
GGCCTGGTCTCGGTCAGCGGATCTCGCCCTTCGCGGTTCCGTCATCTGAGCGCGTTACCGTCGTGCGCTTGGTGCTGGGC
CGGTTCGCGGCTTAAGCTCG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MAPDARCYAAPMTATAQKNIRNFSIVAHIDHGKSTLADRLIQLTGGLSEREMSDQVLDNMDIERERGITIKAQTVRLSYK
AQDGETYTLNLIDTPGHVDFAYEVSRSLAAVEGSLLVVDASQGVEAQTLANVYQAIDNNHDIVPVLNKIDLPAAEPEKVK
SQIEDVIGLDASNAIGISAKTGLNIDQVLEAIVTRLPAPVGDREAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGMRIK
MMGADAVYDVDRVGVFTPKMVAMESLGPGEIGFLNGSIKEVADTRVGDTITDDKRPTSEALPGFKPAQPVVFCGLFPVDA
ADFEDLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFNLDLIATAPSVIYQMELTDGSTIDL
HNPADMPDVVKIEEIREPWIRATIMTPDDYLGAVLKLCQDRRGNQIELTYVGARAMVTYDLPLNEVVFDFYDKLKSISKG
YASFDYNITDYRPGELVKMSILVNGEPVDALSMLVHRTRAEFRGRAMCEKLKDLIPQHLFNIPIQAAIGAKVIARETIKA
LRKDVTAKCYGGDVSRKRKLLDKQKEGKKKMRQFGKVEIPQEAFIAALKMDD

Sequences:

>Translated_612_residues
MAPDARCYAAPMTATAQKNIRNFSIVAHIDHGKSTLADRLIQLTGGLSEREMSDQVLDNMDIERERGITIKAQTVRLSYK
AQDGETYTLNLIDTPGHVDFAYEVSRSLAAVEGSLLVVDASQGVEAQTLANVYQAIDNNHDIVPVLNKIDLPAAEPEKVK
SQIEDVIGLDASNAIGISAKTGLNIDQVLEAIVTRLPAPVGDREAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGMRIK
MMGADAVYDVDRVGVFTPKMVAMESLGPGEIGFLNGSIKEVADTRVGDTITDDKRPTSEALPGFKPAQPVVFCGLFPVDA
ADFEDLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFNLDLIATAPSVIYQMELTDGSTIDL
HNPADMPDVVKIEEIREPWIRATIMTPDDYLGAVLKLCQDRRGNQIELTYVGARAMVTYDLPLNEVVFDFYDKLKSISKG
YASFDYNITDYRPGELVKMSILVNGEPVDALSMLVHRTRAEFRGRAMCEKLKDLIPQHLFNIPIQAAIGAKVIARETIKA
LRKDVTAKCYGGDVSRKRKLLDKQKEGKKKMRQFGKVEIPQEAFIAALKMDD
>Mature_611_residues
APDARCYAAPMTATAQKNIRNFSIVAHIDHGKSTLADRLIQLTGGLSEREMSDQVLDNMDIERERGITIKAQTVRLSYKA
QDGETYTLNLIDTPGHVDFAYEVSRSLAAVEGSLLVVDASQGVEAQTLANVYQAIDNNHDIVPVLNKIDLPAAEPEKVKS
QIEDVIGLDASNAIGISAKTGLNIDQVLEAIVTRLPAPVGDREAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGMRIKM
MGADAVYDVDRVGVFTPKMVAMESLGPGEIGFLNGSIKEVADTRVGDTITDDKRPTSEALPGFKPAQPVVFCGLFPVDAA
DFEDLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLGLLHLEIIQERLSREFNLDLIATAPSVIYQMELTDGSTIDLH
NPADMPDVVKIEEIREPWIRATIMTPDDYLGAVLKLCQDRRGNQIELTYVGARAMVTYDLPLNEVVFDFYDKLKSISKGY
ASFDYNITDYRPGELVKMSILVNGEPVDALSMLVHRTRAEFRGRAMCEKLKDLIPQHLFNIPIQAAIGAKVIARETIKAL
RKDVTAKCYGGDVSRKRKLLDKQKEGKKKMRQFGKVEIPQEAFIAALKMDD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=50.4159733777038, Blast_Score=626, Evalue=1e-179,
Organism=Homo sapiens, GI94966754, Length=162, Percent_Identity=42.5925925925926, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=41.0958904109589, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=47.0149253731343, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=47.0149253731343, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=47.0149253731343, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI18390331, Length=172, Percent_Identity=33.1395348837209, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=43.6363636363636, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=43.6363636363636, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=43.6363636363636, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=36.5671641791045, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=36.5671641791045, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI53729339, Length=226, Percent_Identity=30.5309734513274, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI53729337, Length=226, Percent_Identity=30.5309734513274, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI34147630, Length=244, Percent_Identity=29.5081967213115, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788922, Length=593, Percent_Identity=59.0219224283305, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=515, Percent_Identity=27.378640776699, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=38.0645161290323, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790835, Length=154, Percent_Identity=32.4675324675325, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1789559, Length=223, Percent_Identity=29.1479820627803, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=39.8360655737705, Blast_Score=473, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17556745, Length=168, Percent_Identity=37.5, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.8059701492537, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=39.2592592592593, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=158, Percent_Identity=36.0759493670886, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=159, Percent_Identity=35.2201257861635, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=36.0902255639098, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566303, Length=310, Percent_Identity=23.8709677419355, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=46.5890183028286, Blast_Score=548, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=40.9722222222222, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=40.9722222222222, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=159, Percent_Identity=37.7358490566038, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=39.2857142857143, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=170, Percent_Identity=35.8823529411765, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=247, Percent_Identity=27.5303643724696, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=44.5923460898502, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24582462, Length=183, Percent_Identity=34.9726775956284, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=42.4460431654676, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=35.3333333333333, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585711, Length=150, Percent_Identity=35.3333333333333, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585713, Length=150, Percent_Identity=35.3333333333333, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=41.6107382550336, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=35.1851851851852, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363316, Length=311, Percent_Identity=26.6881028938907, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17864358, Length=311, Percent_Identity=26.6881028938907, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28572034, Length=226, Percent_Identity=30.0884955752212, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67507; Mature: 67375

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPDARCYAAPMTATAQKNIRNFSIVAHIDHGKSTLADRLIQLTGGLSEREMSDQVLDNM
CCCCCCEEECCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCC
DIERERGITIKAQTVRLSYKAQDGETYTLNLIDTPGHVDFAYEVSRSLAAVEGSLLVVDA
CCHHHCCCEEEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEC
SQGVEAQTLANVYQAIDNNHDIVPVLNKIDLPAAEPEKVKSQIEDVIGLDASNAIGISAK
CCCCCHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECC
TGLNIDQVLEAIVTRLPAPVGDREAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGMRIK
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
MMGADAVYDVDRVGVFTPKMVAMESLGPGEIGFLNGSIKEVADTRVGDTITDDKRPTSEA
EECCCHHCCHHHCCCCCCHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCHHC
LPGFKPAQPVVFCGLFPVDAADFEDLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLG
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCHHHCCCHHHHHHH
LLHLEIIQERLSREFNLDLIATAPSVIYQMELTDGSTIDLHNPADMPDVVKIEEIREPWI
HHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCEEECCCCCCCCCCEEHHHHCCCCE
RATIMTPDDYLGAVLKLCQDRRGNQIELTYVGARAMVTYDLPLNEVVFDFYDKLKSISKG
EEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCC
YASFDYNITDYRPGELVKMSILVNGEPVDALSMLVHRTRAEFRGRAMCEKLKDLIPQHLF
CCEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIPIQAAIGAKVIARETIKALRKDVTAKCYGGDVSRKRKLLDKQKEGKKKMRQFGKVEIP
CCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
QEAFIAALKMDD
HHHHEEEEECCC
>Mature Secondary Structure 
APDARCYAAPMTATAQKNIRNFSIVAHIDHGKSTLADRLIQLTGGLSEREMSDQVLDNM
CCCCCEEECCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCC
DIERERGITIKAQTVRLSYKAQDGETYTLNLIDTPGHVDFAYEVSRSLAAVEGSLLVVDA
CCHHHCCCEEEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEC
SQGVEAQTLANVYQAIDNNHDIVPVLNKIDLPAAEPEKVKSQIEDVIGLDASNAIGISAK
CCCCCHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECC
TGLNIDQVLEAIVTRLPAPVGDREAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGMRIK
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
MMGADAVYDVDRVGVFTPKMVAMESLGPGEIGFLNGSIKEVADTRVGDTITDDKRPTSEA
EECCCHHCCHHHCCCCCCHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCHHC
LPGFKPAQPVVFCGLFPVDAADFEDLRAAMGKLRLNDASFSFEMETSAALGFGFRCGFLG
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCHHHCCCHHHHHHH
LLHLEIIQERLSREFNLDLIATAPSVIYQMELTDGSTIDLHNPADMPDVVKIEEIREPWI
HHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCEEECCCCCCCCCCEEHHHHCCCCE
RATIMTPDDYLGAVLKLCQDRRGNQIELTYVGARAMVTYDLPLNEVVFDFYDKLKSISKG
EEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCC
YASFDYNITDYRPGELVKMSILVNGEPVDALSMLVHRTRAEFRGRAMCEKLKDLIPQHLF
CCEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIPIQAAIGAKVIARETIKALRKDVTAKCYGGDVSRKRKLLDKQKEGKKKMRQFGKVEIP
CCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
QEAFIAALKMDD
HHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA