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The map label for this gene is yfcG [H]

Identifier: 158421871

GI number: 158421871

Start: 290060

End: 290761

Strand: Reverse

Name: yfcG [H]

Synonym: AZC_0247

Alternate gene names: 158421871

Gene position: 290761-290060 (Counterclockwise)

Preceding gene: 158421876

Following gene: 158421868

Centisome position: 5.41

GC content: 64.25

Gene sequence:

>702_bases
ATGATCGACCTGCACTACTGGCCGACCCCCAACGGCCACAAGATCACCCTCTTCCTCGAAGAGACCGGCCTGCCCTACAC
CATCCACCCGGTGAACATTTCCGCCGGCGACCAGTTCAAGCCCGACTTCCTCAAGATCGCGCCGAACAACCGCATGCCGG
CCATCGTGGACCATGATCCGCCGGGCGGCGGGGCGCCGGTCTCGGTGTTCGAATCGGGCGCCATCCTGCTCTATCTCGCC
GAGAAGACCGGCCAGTTCCTGCCCAAGGATGTGCGCGGGCGCTATCAGGTGCTCCAGTGGCTGTTCTGGCAGATGGGTGG
CCTTGGCCCCATGGCCGGACAGAACCACCATTTCGTGCAGTATGCGCCCGAGAAGATCCCCTACGCCATGGAGCGCTACG
TGAAGGAGACGAACCGTCTCTACGGCGTGCTGAACCGGCGCCTCGAAGGCCGCGACTTCATCGACGACGAGGGCTATTCC
ATCGCCGACATGGCGGCCTATCCCTGGATCGTGCCGCACGAGCGCCAGCAGCAGAAGCTGGAGGACTTCCCGAACCTCAA
GCGCTGGTTCGAGGCCATCAAGGCCCGCCCGGCCACCGAGCGCGCCTATGAGAAGGGCAAGCAGGTGAACACGACCCCGA
CCGTGAACGAAAACAGCCGCTCGGTTCTGTTCGGCCAGACGGCGGCGAACGTGAAGGCGTGA

Upstream 100 bases:

>100_bases
GCTGCGAGGGTGGTTTCATGCCGCACGCGAATGGGCCAATCTGCGGCCACCGTTGCGTCGCCGGACCTTTCCCGAGCGGC
GCCCCACCCGGAGACCCGGC

Downstream 100 bases:

>100_bases
TGTGGAGAAGCGCCCCGCGCTGTGGCTGTGGGGCACTTCTTTTTTTGCGATGCTTGGGGGAGAGGGTCCGACATGCCCCC
TCCCCGGCCCTCCCCCGCAA

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MIDLHYWPTPNGHKITLFLEETGLPYTIHPVNISAGDQFKPDFLKIAPNNRMPAIVDHDPPGGGAPVSVFESGAILLYLA
EKTGQFLPKDVRGRYQVLQWLFWQMGGLGPMAGQNHHFVQYAPEKIPYAMERYVKETNRLYGVLNRRLEGRDFIDDEGYS
IADMAAYPWIVPHERQQQKLEDFPNLKRWFEAIKARPATERAYEKGKQVNTTPTVNENSRSVLFGQTAANVKA

Sequences:

>Translated_233_residues
MIDLHYWPTPNGHKITLFLEETGLPYTIHPVNISAGDQFKPDFLKIAPNNRMPAIVDHDPPGGGAPVSVFESGAILLYLA
EKTGQFLPKDVRGRYQVLQWLFWQMGGLGPMAGQNHHFVQYAPEKIPYAMERYVKETNRLYGVLNRRLEGRDFIDDEGYS
IADMAAYPWIVPHERQQQKLEDFPNLKRWFEAIKARPATERAYEKGKQVNTTPTVNENSRSVLFGQTAANVKA
>Mature_233_residues
MIDLHYWPTPNGHKITLFLEETGLPYTIHPVNISAGDQFKPDFLKIAPNNRMPAIVDHDPPGGGAPVSVFESGAILLYLA
EKTGQFLPKDVRGRYQVLQWLFWQMGGLGPMAGQNHHFVQYAPEKIPYAMERYVKETNRLYGVLNRRLEGRDFIDDEGYS
IADMAAYPWIVPHERQQQKLEDFPNLKRWFEAIKARPATERAYEKGKQVNTTPTVNENSRSVLFGQTAANVKA

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=207, Percent_Identity=59.4202898550725, Blast_Score=257, Evalue=4e-70,
Organism=Escherichia coli, GI87082195, Length=224, Percent_Identity=38.8392857142857, Blast_Score=154, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324100, Length=233, Percent_Identity=33.0472103004292, Blast_Score=108, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17933730, Length=213, Percent_Identity=29.5774647887324, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571670, Length=175, Percent_Identity=28, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI85725204, Length=206, Percent_Identity=28.6407766990291, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17737923, Length=206, Percent_Identity=28.6407766990291, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864592, Length=210, Percent_Identity=26.1904761904762, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45549270, Length=208, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17864594, Length=219, Percent_Identity=26.9406392694064, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17864596, Length=186, Percent_Identity=26.3440860215054, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17864598, Length=204, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646249, Length=201, Percent_Identity=28.3582089552239, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646251, Length=210, Percent_Identity=27.1428571428571, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26557; Mature: 26557

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLHYWPTPNGHKITLFLEETGLPYTIHPVNISAGDQFKPDFLKIAPNNRMPAIVDHDP
CCCEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
PGGGAPVSVFESGAILLYLAEKTGQFLPKDVRGRYQVLQWLFWQMGGLGPMAGQNHHFVQ
CCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
YAPEKIPYAMERYVKETNRLYGVLNRRLEGRDFIDDEGYSIADMAAYPWIVPHERQQQKL
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHH
EDFPNLKRWFEAIKARPATERAYEKGKQVNTTPTVNENSRSVLFGQTAANVKA
HCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MIDLHYWPTPNGHKITLFLEETGLPYTIHPVNISAGDQFKPDFLKIAPNNRMPAIVDHDP
CCCEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
PGGGAPVSVFESGAILLYLAEKTGQFLPKDVRGRYQVLQWLFWQMGGLGPMAGQNHHFVQ
CCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
YAPEKIPYAMERYVKETNRLYGVLNRRLEGRDFIDDEGYSIADMAAYPWIVPHERQQQKL
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHH
EDFPNLKRWFEAIKARPATERAYEKGKQVNTTPTVNENSRSVLFGQTAANVKA
HCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]