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The map label for this gene is gloB [H]

Identifier: 158421848

GI number: 158421848

Start: 267618

End: 268391

Strand: Reverse

Name: gloB [H]

Synonym: AZC_0224

Alternate gene names: 158421848

Gene position: 268391-267618 (Counterclockwise)

Preceding gene: 158421850

Following gene: 158421847

Centisome position: 5.0

GC content: 68.86

Gene sequence:

>774_bases
ATGCCGCTGGATATCCGCGTCATCCCGTGCCTGAGCGACAATTACGCCGTCCTCCTGCACGATCCCCTCACCGAGGCGAC
CGCCGTGGTGGACGCGCCCGAGCCCGGCCCGATCATGGATGCGCTGGAGCTGGAAGGCTGGGACCTCACCCATATCCTCA
TCACCCATCACCATGCCGACCATGTGGACGGCGTGCTGGCGCTGAAGGCGCAGTATGGCGCCACCGTCGTCGGCCCGAAG
GCCGAGGCCGCCACCATTCCGGCCCTGGATGTGGAGGTGGTGGATGCCGATCCCGTGGGTCTCGGCTCGGCCATCGGCCG
GGTGATCGAGACCCCCGGCCACACGGCGGGCCACATCTCCTTCGTCTTCCCAGAAGAGAAGCTGCTGTTTGCCGGCGACA
CGCTGTTCGTCATGGGCTGCGGCCGGGCGCTGGAATGCGACCCGCCGGTGCTGTGGCACTCGCTCCAGCGCCTGCGCACC
CTGCCCGATGATTTCTCGCTCTATTGCGGGCACGAATACACGGTGGCCAACGGCCGGTTCGCGCTCAGCGTGGATCCCGA
CAACGGCGCCCTGCGCGCCCGCGTCGCTGACGCCGAGGCGGCGCGGCGGGAGGGCAAGCCCACGATTCCGGCGCTGCTCG
GCGACGAGCGTCTCACCAATCCCTTCCTGCGGGCGGACGATCCCGACATGGCCGAACGCCTCGGCCTTGCCGGCGCCGAT
CCCGCTGCCGTCTTCACCGAACTGCGTGCCCGCAAGAATGTGTTCCGGGGCTGA

Upstream 100 bases:

>100_bases
TGCGCCAGTTCCGCCATGTTCCGGTTGTCACGTGGAACGAGGTGGTTTGCGCGCGGCGGCGTGCTACTCAGGGACGCGCC
GCAGCAGGAGGAGATGAAAC

Downstream 100 bases:

>100_bases
ATGATGTCCGACCTCTCCCACCTGTCCGCCGCCGAAATCATCGCAAAACTGGATCTCAAGCCGCATCCGGAAGGCGGCCA
TTATCGCGAGACCTTCCGCG

Product: glyoxalase II

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MPLDIRVIPCLSDNYAVLLHDPLTEATAVVDAPEPGPIMDALELEGWDLTHILITHHHADHVDGVLALKAQYGATVVGPK
AEAATIPALDVEVVDADPVGLGSAIGRVIETPGHTAGHISFVFPEEKLLFAGDTLFVMGCGRALECDPPVLWHSLQRLRT
LPDDFSLYCGHEYTVANGRFALSVDPDNGALRARVADAEAARREGKPTIPALLGDERLTNPFLRADDPDMAERLGLAGAD
PAAVFTELRARKNVFRG

Sequences:

>Translated_257_residues
MPLDIRVIPCLSDNYAVLLHDPLTEATAVVDAPEPGPIMDALELEGWDLTHILITHHHADHVDGVLALKAQYGATVVGPK
AEAATIPALDVEVVDADPVGLGSAIGRVIETPGHTAGHISFVFPEEKLLFAGDTLFVMGCGRALECDPPVLWHSLQRLRT
LPDDFSLYCGHEYTVANGRFALSVDPDNGALRARVADAEAARREGKPTIPALLGDERLTNPFLRADDPDMAERLGLAGAD
PAAVFTELRARKNVFRG
>Mature_256_residues
PLDIRVIPCLSDNYAVLLHDPLTEATAVVDAPEPGPIMDALELEGWDLTHILITHHHADHVDGVLALKAQYGATVVGPKA
EAATIPALDVEVVDADPVGLGSAIGRVIETPGHTAGHISFVFPEEKLLFAGDTLFVMGCGRALECDPPVLWHSLQRLRTL
PDDFSLYCGHEYTVANGRFALSVDPDNGALRARVADAEAARREGKPTIPALLGDERLTNPFLRADDPDMAERLGLAGADP
AAVFTELRARKNVFRG

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=261, Percent_Identity=35.632183908046, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI94538322, Length=261, Percent_Identity=35.632183908046, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI14150041, Length=256, Percent_Identity=36.71875, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI21703352, Length=263, Percent_Identity=35.7414448669202, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI116642887, Length=263, Percent_Identity=35.7414448669202, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI46361987, Length=212, Percent_Identity=36.7924528301887, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI41327741, Length=172, Percent_Identity=29.0697674418605, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786406, Length=255, Percent_Identity=34.1176470588235, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787158, Length=161, Percent_Identity=29.1925465838509, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=255, Percent_Identity=30.5882352941176, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538952, Length=185, Percent_Identity=28.6486486486486, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320478, Length=257, Percent_Identity=28.7937743190661, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324614, Length=264, Percent_Identity=29.9242424242424, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356335, Length=262, Percent_Identity=34.3511450381679, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24667711, Length=262, Percent_Identity=34.3511450381679, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24667703, Length=262, Percent_Identity=34.3511450381679, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI221330176, Length=167, Percent_Identity=30.5389221556886, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 27573; Mature: 27441

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDIRVIPCLSDNYAVLLHDPLTEATAVVDAPEPGPIMDALELEGWDLTHILITHHHAD
CCCEEEEEEEECCCCEEEEECCCCCCEEEEECCCCCCCCHHEECCCCCEEEEEEEECCCC
HVDGVLALKAQYGATVVGPKAEAATIPALDVEVVDADPVGLGSAIGRVIETPGHTAGHIS
CCCCEEEEEECCCEEEECCCCCCEECCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEE
FVFPEEKLLFAGDTLFVMGCGRALECDPPVLWHSLQRLRTLPDDFSLYCGHEYTVANGRF
EEECCCEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCEEEEECCEE
ALSVDPDNGALRARVADAEAARREGKPTIPALLGDERLTNPFLRADDPDMAERLGLAGAD
EEEECCCCCEEEEEECCHHHHHHCCCCCCCCEECCCHHCCCCCCCCCCCHHHHHCCCCCC
PAAVFTELRARKNVFRG
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLDIRVIPCLSDNYAVLLHDPLTEATAVVDAPEPGPIMDALELEGWDLTHILITHHHAD
CCEEEEEEEECCCCEEEEECCCCCCEEEEECCCCCCCCHHEECCCCCEEEEEEEECCCC
HVDGVLALKAQYGATVVGPKAEAATIPALDVEVVDADPVGLGSAIGRVIETPGHTAGHIS
CCCCEEEEEECCCEEEECCCCCCEECCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEE
FVFPEEKLLFAGDTLFVMGCGRALECDPPVLWHSLQRLRTLPDDFSLYCGHEYTVANGRF
EEECCCEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCEEEEECCEE
ALSVDPDNGALRARVADAEAARREGKPTIPALLGDERLTNPFLRADDPDMAERLGLAGAD
EEEECCCCCEEEEEECCHHHHHHCCCCCCCCEECCCHHCCCCCCCCCCCHHHHHCCCCCC
PAAVFTELRARKNVFRG
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]