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The map label for this gene is pyrF [H]

Identifier: 158421783

GI number: 158421783

Start: 189075

End: 189785

Strand: Reverse

Name: pyrF [H]

Synonym: AZC_0159

Alternate gene names: 158421783

Gene position: 189785-189075 (Counterclockwise)

Preceding gene: 158421784

Following gene: 158421782

Centisome position: 3.53

GC content: 68.92

Gene sequence:

>711_bases
TTGACCGACGTCTCCCCGGACATCCGCGACCGTCTCATCGTCGCTCTCGATTATCCCAACGTGGGTGCTGCCGAAGCGCT
CGTCTGGCAACTCGGCGATGCGGTGAGTTTCTACAAGGTCGGTCTGGAGCTTACCGTGGGCGGCGGCCTCGACCTGGCGG
CGGACCTCATCGACGCCGGCAAGAAGGTCTTCCTGGACCTCAAGATGCACGACATCGGCAACACGGTGGAAAAGGCGACC
GCCGCCGCCGCCGAACGGGGCGTTTCGCTGCTGACCGTGCACGCCTACCCGCAGACCATGGCCGCCGCCGCCCGCGGCGC
GAAGGGCAGCGCCCTGCAAGTTTTAGGTGTTACGGTGCTGACGTCCTATGATGAGGCGGATCTCGCTGCGGCAGGCTATG
CTCAGGGTGTGACGGAAACCGTGCGGCGCCGGGCCATCCAGGCCCAGGAGGCGGGCATTGCAGGCGTGATCTGCGCGCCC
ACCGACGCGGCCATGGTCCGCGCGGCGACCAGCCCGGATTTCCAGATCGTGACCCCGGGCGTTCGCCCCGCTGGCAGCGC
GGCCGGCGACCAGAAGCGGTTTGCCACGCCCGCCGATGCCATGGCGGCGGGGGCGAACCGCATCGTCGTGGGACGACCGA
TCACCGGCGCGGCGGACCCGTCCGCAGCAGCCCGGAGCATCCTGGAGGAACTGGCAAAAGCGGCCGGGTGA

Upstream 100 bases:

>100_bases
CGTTCCCACGACCAGCCACCGGTGGGGGGTGGATGCTGGTTCCGCTCTACGCACACCACGGCGCCCGACCGGTGGCGCCC
GATCGAAGAGGCTGCCTGAC

Downstream 100 bases:

>100_bases
TCCCGCGCCGCGCAATGGGGCGAGGCGCACATGAGCGAGTTGGGGACGCCGAAGGGTTACTGGGTCGCGCGGGTCGATGT
CCACGATCCCGAAGGCTACC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTDVSPDIRDRLIVALDYPNVGAAEALVWQLGDAVSFYKVGLELTVGGGLDLAADLIDAGKKVFLDLKMHDIGNTVEKAT
AAAAERGVSLLTVHAYPQTMAAAARGAKGSALQVLGVTVLTSYDEADLAAAGYAQGVTETVRRRAIQAQEAGIAGVICAP
TDAAMVRAATSPDFQIVTPGVRPAGSAAGDQKRFATPADAMAAGANRIVVGRPITGAADPSAAARSILEELAKAAG

Sequences:

>Translated_236_residues
MTDVSPDIRDRLIVALDYPNVGAAEALVWQLGDAVSFYKVGLELTVGGGLDLAADLIDAGKKVFLDLKMHDIGNTVEKAT
AAAAERGVSLLTVHAYPQTMAAAARGAKGSALQVLGVTVLTSYDEADLAAAGYAQGVTETVRRRAIQAQEAGIAGVICAP
TDAAMVRAATSPDFQIVTPGVRPAGSAAGDQKRFATPADAMAAGANRIVVGRPITGAADPSAAARSILEELAKAAG
>Mature_235_residues
TDVSPDIRDRLIVALDYPNVGAAEALVWQLGDAVSFYKVGLELTVGGGLDLAADLIDAGKKVFLDLKMHDIGNTVEKATA
AAAERGVSLLTVHAYPQTMAAAARGAKGSALQVLGVTVLTSYDEADLAAAGYAQGVTETVRRRAIQAQEAGIAGVICAPT
DAAMVRAATSPDFQIVTPGVRPAGSAAGDQKRFATPADAMAAGANRIVVGRPITGAADPSAAARSILEELAKAAG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=230, Percent_Identity=38.695652173913, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 23968; Mature: 23837

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVSPDIRDRLIVALDYPNVGAAEALVWQLGDAVSFYKVGLELTVGGGLDLAADLIDAG
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCHHEEEEEEEEEEECCCCHHHHHHHHCC
KKVFLDLKMHDIGNTVEKATAAAAERGVSLLTVHAYPQTMAAAARGAKGSALQVLGVTVL
CEEEEEEEEHHHCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEEEEE
TSYDEADLAAAGYAQGVTETVRRRAIQAQEAGIAGVICAPTDAAMVRAATSPDFQIVTPG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHEEECCCCCCEEEECC
VRPAGSAAGDQKRFATPADAMAAGANRIVVGRPITGAADPSAAARSILEELAKAAG
CCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDVSPDIRDRLIVALDYPNVGAAEALVWQLGDAVSFYKVGLELTVGGGLDLAADLIDAG
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCHHEEEEEEEEEEECCCCHHHHHHHHCC
KKVFLDLKMHDIGNTVEKATAAAAERGVSLLTVHAYPQTMAAAARGAKGSALQVLGVTVL
CEEEEEEEEHHHCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEEEEE
TSYDEADLAAAGYAQGVTETVRRRAIQAQEAGIAGVICAPTDAAMVRAATSPDFQIVTPG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHEEECCCCCCEEEECC
VRPAGSAAGDQKRFATPADAMAAGANRIVVGRPITGAADPSAAARSILEELAKAAG
CCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA