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The map label for this gene is nudF [H]

Identifier: 158421752

GI number: 158421752

Start: 155742

End: 156386

Strand: Reverse

Name: nudF [H]

Synonym: AZC_0128

Alternate gene names: 158421752

Gene position: 156386-155742 (Counterclockwise)

Preceding gene: 158421753

Following gene: 158421747

Centisome position: 2.91

GC content: 68.22

Gene sequence:

>645_bases
GTGACGAAGACGCCCTTTCAGGACGAACCGGCCCGTATCGACATTCTCGACGAAACGCTGATCTACAAGGGCTTCCGCCG
TTTCGAGCGCTATGACCTCCGCCTACATACGCCGGGCGCCGAGCCTGTGGTGCAGACCCGCGAAATCCTGCGGGTCGGCG
GGGCGGTCGGCATCCTCGCCATTGATCCGGTCCGCGATGCCATCGTGCTGCTGCGCCAGTTCCGCCTCACGGCGCACATG
GCAACCGGACGGGGCGAGCTCATCGAAATCCCGGCCGGGCTGATCGAGCCCGGAGAAGACCCCGCGCTCACCGCGCGGCG
GGAATGCATCGAGGAAACCGGGCTTGACCCGGTCGAGGTGGACTTCCTCTTCTCCTGCCTCGTCTCGCCGGGCGCCGTGG
ATGAGATGGCGTGGTTCTACCTCGCCTTCGTCGATGCCTCGAACCTGCCGGAGACCACCGGCGCGGCGGACGAGACGGAA
GTCATCCGCCCCTTCCTCGTGCCGCTCGATCGGGCCATTGCGGCACTCGACGACGGATCTTCCCTCGCCAACGGCTATCT
GCGCCTCGCCCTCATGTGGATCGCCCTGCACCGCGCCGACGTCGCCGCCCGCGGCGAGGCCTGGCTGGCCCGCCGGGCAG
CCTGA

Upstream 100 bases:

>100_bases
CGGCGCGGGAGGTGTTCGAGCATCCCCGCGAGGCCTATACGCAGGCGCTGTTCGCCGCCGCCTTCAACATCGAGACGGCC
CATGCGGGAGTGACCGCCCA

Downstream 100 bases:

>100_bases
GGGCGCGCGCAGCTCCCGTGCTCAGGCCGCCGCCGCATTGGCGGCGAGGACTTCGCGGCTGAGGTCCAGCCACGCGAGGG
CGGCGCGGGAGAGGTAGCCG

Product: nucleoside diphosphate pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTKTPFQDEPARIDILDETLIYKGFRRFERYDLRLHTPGAEPVVQTREILRVGGAVGILAIDPVRDAIVLLRQFRLTAHM
ATGRGELIEIPAGLIEPGEDPALTARRECIEETGLDPVEVDFLFSCLVSPGAVDEMAWFYLAFVDASNLPETTGAADETE
VIRPFLVPLDRAIAALDDGSSLANGYLRLALMWIALHRADVAARGEAWLARRAA

Sequences:

>Translated_214_residues
MTKTPFQDEPARIDILDETLIYKGFRRFERYDLRLHTPGAEPVVQTREILRVGGAVGILAIDPVRDAIVLLRQFRLTAHM
ATGRGELIEIPAGLIEPGEDPALTARRECIEETGLDPVEVDFLFSCLVSPGAVDEMAWFYLAFVDASNLPETTGAADETE
VIRPFLVPLDRAIAALDDGSSLANGYLRLALMWIALHRADVAARGEAWLARRAA
>Mature_213_residues
TKTPFQDEPARIDILDETLIYKGFRRFERYDLRLHTPGAEPVVQTREILRVGGAVGILAIDPVRDAIVLLRQFRLTAHMA
TGRGELIEIPAGLIEPGEDPALTARRECIEETGLDPVEVDFLFSCLVSPGAVDEMAWFYLAFVDASNLPETTGAADETEV
IRPFLVPLDRAIAALDDGSSLANGYLRLALMWIALHRADVAARGEAWLARRAA

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=187, Percent_Identity=34.2245989304813, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1788810, Length=169, Percent_Identity=28.9940828402367, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23597; Mature: 23466

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTPFQDEPARIDILDETLIYKGFRRFERYDLRLHTPGAEPVVQTREILRVGGAVGILA
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHCCCEEEEE
IDPVRDAIVLLRQFRLTAHMATGRGELIEIPAGLIEPGEDPALTARRECIEETGLDPVEV
ECHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
DFLFSCLVSPGAVDEMAWFYLAFVDASNLPETTGAADETEVIRPFLVPLDRAIAALDDGS
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
SLANGYLRLALMWIALHRADVAARGEAWLARRAA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
TKTPFQDEPARIDILDETLIYKGFRRFERYDLRLHTPGAEPVVQTREILRVGGAVGILA
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHCCCEEEEE
IDPVRDAIVLLRQFRLTAHMATGRGELIEIPAGLIEPGEDPALTARRECIEETGLDPVEV
ECHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
DFLFSCLVSPGAVDEMAWFYLAFVDASNLPETTGAADETEVIRPFLVPLDRAIAALDDGS
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
SLANGYLRLALMWIALHRADVAARGEAWLARRAA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]