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The map label for this gene is glcD [H]

Identifier: 158421707

GI number: 158421707

Start: 101086

End: 102645

Strand: Reverse

Name: glcD [H]

Synonym: AZC_0083

Alternate gene names: 158421707

Gene position: 102645-101086 (Counterclockwise)

Preceding gene: 158421711

Following gene: 158421706

Centisome position: 1.91

GC content: 66.6

Gene sequence:

>1560_bases
ATGCCGGATTTTCGCATCGAAACGATTATAGTCGCGGCAACGAGAACCATTCTAGGGACGACCGCCATGACGGGCCTCAG
GCTTCCGGAAGCGGACGCGAGCGTGCTGGCACGCCGGGACGAGATCATCACCGCGCTGCGCGCCATCGTGCCGGGAGAAG
GCGTCATCGATCGCGAGCGGGAGATGCGTCCTTATGAATCGGACGCGCTGACCGCCTATCGCCAGCTTCCCCTCGTGGTG
GTGCTGCCCTCCAATACGCAGCAGGTCTCACAGGTGCTGCGCTATTGCCACGAGAACAACATCCGCGTGGTGCCGCGCGG
GGCTGGCACCTCCCTCTCCGGCGGCGCGCTGCCGCTTCAGGATGCGGTGCTGCTCGGCATGGGCAAGTTCAACCGCGTGC
TGGACATCGACTTTCCCAACCGCTGCGCGGTGGTGCAGCCGGGCGTCACCAACCTCGGCATCACGAAAGCCGTAGAGCAT
GAGGGCTTCTATTACGCCCCCGACCCCTCCTCGCAGATCGCCTGCACCATCGGCGGCAATGTGGGCGAGAATTCCGGCGG
CGTGCACTGCCTGAAATACGGCCTCACCACCAACAATGTGCTGGGCGTCGAGCTGGCGCTCATCACCGGCGAGATCGTGC
GTCTCGGCGGCAAGCATCTGGACGCGGGTGGCCTCGACCTGCTCAGCGTCGTCATCGGCTCGGAAGGGCTGCTGGGCGTC
GTCACCGAAGTCACCGTCCGCCTGCTCAAGAAGCCGGACACGGCGCGCGCCGTGCTGGTGGGCTTTCCCACCTCCGAGGA
TGCGGGCGAATGCGTGGCCCGCACCATCGCCGCCGGCATCATCCCCGCCGGCATCGAGATGATGGACAAGGACGCTATCG
CCGCCGCCGAAGCCTTCGTGAAGGTGGGCTATCCGCTCGATGTGGAAGCCCTGCTCATCATCGAGCTGGACGGACCGGAA
GCGGAATGCGCGCACCTGCTGGAAGAGGTCGCCGCCATCGCGCGCGGCTGCAACGCCTCCACCTTGCGCGTGTCGCAGAC
GGAAGAGGAGCGCATGGGCTTCTGGGCCGGGCGCAAGGCCGCCTTCCCGGCGGTGGGGCGCATCTCGCCGGACTATCTCT
GCATGGACGGCACCATCCCGCGCAAGGCACTGCCGCTGGTGCTCTCGCGGATGCGCGAACTCTCCGAAAAACACGGCCTC
AAGTGCGCCAACGTCTTCCACGCGGGCGACGGCAATCTGCACCCGCTCATCCTCTATGACGCGAACAAGGGCGAGATGGA
CGCGGCCGAGGCCTTCGGCGCCGATATCCTGCGCCTGTGCGTGGAGGTGGGCGGCGTGCTCACCGGCGAGCATGGCGTGG
GCATCGAGAAGCGCGACCTGATGCCGGAGATGTTCGACGAGGTGGACCTGGCCCACCAGCAGCGTCTGAAATGCGCCTTC
GACCCGGAAAGCCTGCTCAATCCGGGCAAGGTGTTCCCCACGCTGCACCGCTGCGCCGAGCTGGGCCGGATGCATGTGGC
GGGCGGCGCGCTGCCCTTCCCCGACCTTCCCCGTTTCTGA

Upstream 100 bases:

>100_bases
ATCATCGGTCGCACCTCGGGCGAGGAACGGCGGGTTGCGGTGGTGTCTCGGCGGATTGTTTCCATGGCGCTATTTATTTT
AGCCGCTGCGGCAATAATCA

Downstream 100 bases:

>100_bases
CGCCGTGGGCGCATCATGACCGACATTCTCACCGTGGCGGACGAAGCCGGCCTCGTGGAGGCCGTGCGCTCGGCGCTGGA
AGCCCGCAAGACGCTGGAGG

Product: glycolate oxidase subunit protein

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MPDFRIETIIVAATRTILGTTAMTGLRLPEADASVLARRDEIITALRAIVPGEGVIDREREMRPYESDALTAYRQLPLVV
VLPSNTQQVSQVLRYCHENNIRVVPRGAGTSLSGGALPLQDAVLLGMGKFNRVLDIDFPNRCAVVQPGVTNLGITKAVEH
EGFYYAPDPSSQIACTIGGNVGENSGGVHCLKYGLTTNNVLGVELALITGEIVRLGGKHLDAGGLDLLSVVIGSEGLLGV
VTEVTVRLLKKPDTARAVLVGFPTSEDAGECVARTIAAGIIPAGIEMMDKDAIAAAEAFVKVGYPLDVEALLIIELDGPE
AECAHLLEEVAAIARGCNASTLRVSQTEEERMGFWAGRKAAFPAVGRISPDYLCMDGTIPRKALPLVLSRMRELSEKHGL
KCANVFHAGDGNLHPLILYDANKGEMDAAEAFGADILRLCVEVGGVLTGEHGVGIEKRDLMPEMFDEVDLAHQQRLKCAF
DPESLLNPGKVFPTLHRCAELGRMHVAGGALPFPDLPRF

Sequences:

>Translated_519_residues
MPDFRIETIIVAATRTILGTTAMTGLRLPEADASVLARRDEIITALRAIVPGEGVIDREREMRPYESDALTAYRQLPLVV
VLPSNTQQVSQVLRYCHENNIRVVPRGAGTSLSGGALPLQDAVLLGMGKFNRVLDIDFPNRCAVVQPGVTNLGITKAVEH
EGFYYAPDPSSQIACTIGGNVGENSGGVHCLKYGLTTNNVLGVELALITGEIVRLGGKHLDAGGLDLLSVVIGSEGLLGV
VTEVTVRLLKKPDTARAVLVGFPTSEDAGECVARTIAAGIIPAGIEMMDKDAIAAAEAFVKVGYPLDVEALLIIELDGPE
AECAHLLEEVAAIARGCNASTLRVSQTEEERMGFWAGRKAAFPAVGRISPDYLCMDGTIPRKALPLVLSRMRELSEKHGL
KCANVFHAGDGNLHPLILYDANKGEMDAAEAFGADILRLCVEVGGVLTGEHGVGIEKRDLMPEMFDEVDLAHQQRLKCAF
DPESLLNPGKVFPTLHRCAELGRMHVAGGALPFPDLPRF
>Mature_518_residues
PDFRIETIIVAATRTILGTTAMTGLRLPEADASVLARRDEIITALRAIVPGEGVIDREREMRPYESDALTAYRQLPLVVV
LPSNTQQVSQVLRYCHENNIRVVPRGAGTSLSGGALPLQDAVLLGMGKFNRVLDIDFPNRCAVVQPGVTNLGITKAVEHE
GFYYAPDPSSQIACTIGGNVGENSGGVHCLKYGLTTNNVLGVELALITGEIVRLGGKHLDAGGLDLLSVVIGSEGLLGVV
TEVTVRLLKKPDTARAVLVGFPTSEDAGECVARTIAAGIIPAGIEMMDKDAIAAAEAFVKVGYPLDVEALLIIELDGPEA
ECAHLLEEVAAIARGCNASTLRVSQTEEERMGFWAGRKAAFPAVGRISPDYLCMDGTIPRKALPLVLSRMRELSEKHGLK
CANVFHAGDGNLHPLILYDANKGEMDAAEAFGADILRLCVEVGGVLTGEHGVGIEKRDLMPEMFDEVDLAHQQRLKCAFD
PESLLNPGKVFPTLHRCAELGRMHVAGGALPFPDLPRF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=459, Percent_Identity=28.322440087146, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI37595754, Length=482, Percent_Identity=27.1784232365145, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI119964728, Length=476, Percent_Identity=29.4117647058824, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI4501993, Length=230, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789351, Length=493, Percent_Identity=55.7809330628803, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1787977, Length=501, Percent_Identity=24.750499001996, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI48994907, Length=221, Percent_Identity=28.9592760180996, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71992373, Length=461, Percent_Identity=28.8503253796095, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71992381, Length=458, Percent_Identity=28.6026200873362, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71992392, Length=359, Percent_Identity=28.4122562674095, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17534361, Length=452, Percent_Identity=25, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17556096, Length=229, Percent_Identity=24.8908296943231, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320027, Length=433, Percent_Identity=28.8683602771363, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320764, Length=469, Percent_Identity=25.1599147121535, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320023, Length=447, Percent_Identity=27.5167785234899, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18921117, Length=427, Percent_Identity=28.8056206088993, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639277, Length=427, Percent_Identity=28.8056206088993, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639275, Length=427, Percent_Identity=28.8056206088993, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24653753, Length=232, Percent_Identity=28.0172413793103, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 55503; Mature: 55372

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFRIETIIVAATRTILGTTAMTGLRLPEADASVLARRDEIITALRAIVPGEGVIDRER
CCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EMRPYESDALTAYRQLPLVVVLPSNTQQVSQVLRYCHENNIRVVPRGAGTSLSGGALPLQ
HCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHH
DAVLLGMGKFNRVLDIDFPNRCAVVQPGVTNLGITKAVEHEGFYYAPDPSSQIACTIGGN
HHHHHCCCCCCCEEEECCCCCCEEECCCCCCCCCHHHHCCCCEEECCCCCCCEEEEECCC
VGENSGGVHCLKYGLTTNNVLGVELALITGEIVRLGGKHLDAGGLDLLSVVIGSEGLLGV
CCCCCCCEEEEEECCCCCCEEEEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHH
VTEVTVRLLKKPDTARAVLVGFPTSEDAGECVARTIAAGIIPAGIEMMDKDAIAAAEAFV
HHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KVGYPLDVEALLIIELDGPEAECAHLLEEVAAIARGCNASTLRVSQTEEERMGFWAGRKA
HCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCC
AFPAVGRISPDYLCMDGTIPRKALPLVLSRMRELSEKHGLKCANVFHAGDGNLHPLILYD
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEE
ANKGEMDAAEAFGADILRLCVEVGGVLTGEHGVGIEKRDLMPEMFDEVDLAHQQRLKCAF
CCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHCCHHHHHHHHHHHHHHCEECC
DPESLLNPGKVFPTLHRCAELGRMHVAGGALPFPDLPRF
CHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
PDFRIETIIVAATRTILGTTAMTGLRLPEADASVLARRDEIITALRAIVPGEGVIDRER
CCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EMRPYESDALTAYRQLPLVVVLPSNTQQVSQVLRYCHENNIRVVPRGAGTSLSGGALPLQ
HCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHH
DAVLLGMGKFNRVLDIDFPNRCAVVQPGVTNLGITKAVEHEGFYYAPDPSSQIACTIGGN
HHHHHCCCCCCCEEEECCCCCCEEECCCCCCCCCHHHHCCCCEEECCCCCCCEEEEECCC
VGENSGGVHCLKYGLTTNNVLGVELALITGEIVRLGGKHLDAGGLDLLSVVIGSEGLLGV
CCCCCCCEEEEEECCCCCCEEEEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHH
VTEVTVRLLKKPDTARAVLVGFPTSEDAGECVARTIAAGIIPAGIEMMDKDAIAAAEAFV
HHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KVGYPLDVEALLIIELDGPEAECAHLLEEVAAIARGCNASTLRVSQTEEERMGFWAGRKA
HCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCC
AFPAVGRISPDYLCMDGTIPRKALPLVLSRMRELSEKHGLKCANVFHAGDGNLHPLILYD
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEE
ANKGEMDAAEAFGADILRLCVEVGGVLTGEHGVGIEKRDLMPEMFDEVDLAHQQRLKCAF
CCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHCCHHHHHHHHHHHHHHCEECC
DPESLLNPGKVFPTLHRCAELGRMHVAGGALPFPDLPRF
CHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]