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The map label for this gene is pnp [H]

Identifier: 158421687

GI number: 158421687

Start: 79264

End: 81534

Strand: Reverse

Name: pnp [H]

Synonym: AZC_0063

Alternate gene names: 158421687

Gene position: 81534-79264 (Counterclockwise)

Preceding gene: 158421688

Following gene: 158421683

Centisome position: 1.52

GC content: 66.36

Gene sequence:

>2271_bases
GTGCTCCCCTCCGCCCCCTGCGGCGGTGGGGCGGCGCGTGCGTCCCGCCGTCTTGCTCATGGCTCTCCTTCTTCGTCTGA
GAAGAAAGAGACTTTCATGTTCGACATCCATCGCGAAGAGCTGGATTGGGGTGGCCGCACGCTGGTGCTGGAGACGGGCA
AGATGGCCCGTCAGGCCGACGGCGCCGTCTTCGCCTCCTATGGCGACACCACCGTCCTCGCCACCGTCGTCTCGGCCCGT
GAGCCCAAGCCCGGCCAGGACTTCTTCCCCCTGACGGTGAACTATCAGGAAAAGACCTACGCGGCCGGCCGCATTCCCGG
CGGCTATTTCAAGCGCGAGGGCCGTCCCTCCGAGAAGGAGACGCTGGTCTCCCGCCTCATCGACCGCCCCATCCGCCCGC
TGTTCGCGGACGGCTACAAGAACGACACCCAGGTGGTGGTCACCGTTCTCGCCCATGACCTCGAGAACGATCCGGACGTG
CTGTCCATGGTCGCCGCCTCCGCGGCCCTGACGCTCTCCGGCGTGCCCTTCATGGGCCCCATCGGCGGCGCCCGCGTCGG
CTTCGTCGGCGACGAATACGTGCTGAACCCCACCATCGAAGAGCTGAAGGACAGCGCCCTCGACCTCGTGGTCGCAGGCA
CCTCCGACGCCGTGCTCATGGTGGAATCCGAAGCCAAGGAGCTCTCCGAAGAGGTAATGCTCGGCGCCGTGATGTTCGGC
CACCGCCACTTCCAGCCGGTGATCGAGGCCATCATCCGCCTCGCCGAGAAGGCTGCCAAGGAGCCGCGCCCCTTCGAGCC
GGTGGATCACTCCGCCCTCGAGGCCAAGATCCGCGAGATCGCCGAGGCCGACCTGCGCGCCGCCTACAAGATCAAGCAGA
AGCAGGCCCGCTACGAGGCGGTCGGCGCTGCCAAGTCCAAGGTGAAGAAGTATTTCGAGCAGCTGGCCATCGACGGTCAG
GAAGCCCCGAAGGCCCAGGACGTGGCCGAGGTGCTGAAGGGTCTCGAAGCCAAGATCGTGCGCTGGGCCATCCTCGATGA
CGGCATCCGCATCGACGGCCGCGACGTGAAGACCGTCCGCCCGATCGTGTCGGAAGTCGGCATCCTGCCCCGCGCCCACG
GTTCGGCCCTGTTCACCCGCGGCGAGACGCAGGCCCTCGTGGTCGCCACCCTCGGCACCGGCGAGGACGAGCAGTTCATC
GACAGCCTGGAAGGCACCTACAAGGAGCACTTCCTGCTGCACTACAACTTCCCGCCCTTCTCCGTGGGCGAGACCGGCCG
CATGGGTTCGCCCGGCCGTCGTGAGATCGGCCACGGCAAGCTCGCCTGGCGCGCCATCCACCCGATGCTGCCGGCCAAGC
ACGAGTTCCCCTACACCCTGCGCGTGGTGTCGGAGATCCTCGAGTCCAACGGCTCGTCCTCCATGGCCACCGTCTGCGGC
ACCTCGCTGTCGCTGATGGACGCCGGCGTGCCGCTGAAGAGCCCCGTCGCGGGCATCGCCATGGGCCTGATCCTGGAAGG
CGAGAAGTTCGCGGTCCTGTCGGACATCCTGGGCGACGAGGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGCACGG
AATCGGGCGTCACCGCCCTCCAGATGGACATCAAGATCGCCGGCATCACCGAGGAGATCATGAAGGTCGCCCTCGCCCAG
GCGAAGGATGGCCGCGCGCACATCCTCGGCGAGATGTCCAAGGCCCTGACCTCGGCCCGTGCGGAACTCGGCGAGCACGC
CCCGCGCATCGAGGTGATGAAGATCGCCGTGGACAAGATCCGCGAAGTGATCGGCTCCGGCGGCAAGGTGATCCGCGAGA
TCGTCGAGAAGACCGGCGCCAAGATCAACATCGAGGACGACGGCACCATCAAGATCGCTTCCGCCTCCGGCGACAGCATC
AAGGCCGCCATCAACTGGATCAAGTCCATCGCCTCGGAGCCCGAGGTCGGCGCGATCTATGACGGCACGGTCGTGAAGGT
GGTGGACTTCGGCGCCTTCGTGAACTTCTTCGGTTCCAAGGACGGCCTGGTCCACATTTCGCAGCTCTCCAAGGAGCGCG
TGGCCAAGAGCTCCGACGTGGTCAAGGAAGGCGACAAGGTCAAGGTCAAGCTGGTCGGCTTCGACGAGCGCGGCAAGACC
CGCCTGTCCATGAAGGCGGTCGACCAGACCACCGGCGAGGATCTCGAAGCCAAGGCCAAGTCCGAGAAGGACGCCGCCAA
GGAAGGCGACGAGGCCGGCGCTGCCGAGTGA

Upstream 100 bases:

>100_bases
ACGGCGCTTTCGTCGGGAGCCACAGACGCAGCGGGCATGGGGCTCGCCGCGCATCGCGAAGACTTAAGGAAAGTCATGGC
AGGATCGCCGGACGCATGCC

Downstream 100 bases:

>100_bases
CGTTGACGGCTCCGGGCCTGCCCGGAGCCTCTTCGGCCGCTGAATGAGGCGGATGAATCAGAAAGGGCGGTCCCTTCCGG
GGCCGCCCTTTTTCGTCTCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 756; Mature: 756

Protein sequence:

>756_residues
MLPSAPCGGGAARASRRLAHGSPSSSEKKETFMFDIHREELDWGGRTLVLETGKMARQADGAVFASYGDTTVLATVVSAR
EPKPGQDFFPLTVNYQEKTYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFADGYKNDTQVVVTVLAHDLENDPDV
LSMVAASAALTLSGVPFMGPIGGARVGFVGDEYVLNPTIEELKDSALDLVVAGTSDAVLMVESEAKELSEEVMLGAVMFG
HRHFQPVIEAIIRLAEKAAKEPRPFEPVDHSALEAKIREIAEADLRAAYKIKQKQARYEAVGAAKSKVKKYFEQLAIDGQ
EAPKAQDVAEVLKGLEAKIVRWAILDDGIRIDGRDVKTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTGEDEQFI
DSLEGTYKEHFLLHYNFPPFSVGETGRMGSPGRREIGHGKLAWRAIHPMLPAKHEFPYTLRVVSEILESNGSSSMATVCG
TSLSLMDAGVPLKSPVAGIAMGLILEGEKFAVLSDILGDEDHLGDMDFKVAGTESGVTALQMDIKIAGITEEIMKVALAQ
AKDGRAHILGEMSKALTSARAELGEHAPRIEVMKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKIASASGDSI
KAAINWIKSIASEPEVGAIYDGTVVKVVDFGAFVNFFGSKDGLVHISQLSKERVAKSSDVVKEGDKVKVKLVGFDERGKT
RLSMKAVDQTTGEDLEAKAKSEKDAAKEGDEAGAAE

Sequences:

>Translated_756_residues
MLPSAPCGGGAARASRRLAHGSPSSSEKKETFMFDIHREELDWGGRTLVLETGKMARQADGAVFASYGDTTVLATVVSAR
EPKPGQDFFPLTVNYQEKTYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFADGYKNDTQVVVTVLAHDLENDPDV
LSMVAASAALTLSGVPFMGPIGGARVGFVGDEYVLNPTIEELKDSALDLVVAGTSDAVLMVESEAKELSEEVMLGAVMFG
HRHFQPVIEAIIRLAEKAAKEPRPFEPVDHSALEAKIREIAEADLRAAYKIKQKQARYEAVGAAKSKVKKYFEQLAIDGQ
EAPKAQDVAEVLKGLEAKIVRWAILDDGIRIDGRDVKTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTGEDEQFI
DSLEGTYKEHFLLHYNFPPFSVGETGRMGSPGRREIGHGKLAWRAIHPMLPAKHEFPYTLRVVSEILESNGSSSMATVCG
TSLSLMDAGVPLKSPVAGIAMGLILEGEKFAVLSDILGDEDHLGDMDFKVAGTESGVTALQMDIKIAGITEEIMKVALAQ
AKDGRAHILGEMSKALTSARAELGEHAPRIEVMKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKIASASGDSI
KAAINWIKSIASEPEVGAIYDGTVVKVVDFGAFVNFFGSKDGLVHISQLSKERVAKSSDVVKEGDKVKVKLVGFDERGKT
RLSMKAVDQTTGEDLEAKAKSEKDAAKEGDEAGAAE
>Mature_756_residues
MLPSAPCGGGAARASRRLAHGSPSSSEKKETFMFDIHREELDWGGRTLVLETGKMARQADGAVFASYGDTTVLATVVSAR
EPKPGQDFFPLTVNYQEKTYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFADGYKNDTQVVVTVLAHDLENDPDV
LSMVAASAALTLSGVPFMGPIGGARVGFVGDEYVLNPTIEELKDSALDLVVAGTSDAVLMVESEAKELSEEVMLGAVMFG
HRHFQPVIEAIIRLAEKAAKEPRPFEPVDHSALEAKIREIAEADLRAAYKIKQKQARYEAVGAAKSKVKKYFEQLAIDGQ
EAPKAQDVAEVLKGLEAKIVRWAILDDGIRIDGRDVKTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTGEDEQFI
DSLEGTYKEHFLLHYNFPPFSVGETGRMGSPGRREIGHGKLAWRAIHPMLPAKHEFPYTLRVVSEILESNGSSSMATVCG
TSLSLMDAGVPLKSPVAGIAMGLILEGEKFAVLSDILGDEDHLGDMDFKVAGTESGVTALQMDIKIAGITEEIMKVALAQ
AKDGRAHILGEMSKALTSARAELGEHAPRIEVMKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKIASASGDSI
KAAINWIKSIASEPEVGAIYDGTVVKVVDFGAFVNFFGSKDGLVHISQLSKERVAKSSDVVKEGDKVKVKLVGFDERGKT
RLSMKAVDQTTGEDLEAKAKSEKDAAKEGDEAGAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=713, Percent_Identity=38.0084151472651, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=50, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI21361576, Length=93, Percent_Identity=44.0860215053763, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI145693187, Length=689, Percent_Identity=58.055152394775, Blast_Score=783, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=709, Percent_Identity=35.2609308885755, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535281, Length=87, Percent_Identity=51.7241379310345, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320850, Length=126, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362905, Length=711, Percent_Identity=38.6779184247539, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=711, Percent_Identity=38.6779184247539, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=711, Percent_Identity=38.6779184247539, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=661, Percent_Identity=38.2753403933434, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI20129977, Length=100, Percent_Identity=42, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 81272; Mature: 81272

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSAPCGGGAARASRRLAHGSPSSSEKKETFMFDIHREELDWGGRTLVLETGKMARQAD
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEEECCCHHHHCC
GAVFASYGDTTVLATVVSAREPKPGQDFFPLTVNYQEKTYAAGRIPGGYFKREGRPSEKE
CCEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEECCHHHEECCCCCCCHHCCCCCCCHHH
TLVSRLIDRPIRPLFADGYKNDTQVVVTVLAHDLENDPDVLSMVAASAALTLSGVPFMGP
HHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCCCCC
IGGARVGFVGDEYVLNPTIEELKDSALDLVVAGTSDAVLMVESEAKELSEEVMLGAVMFG
CCCCEEEECCCCEEECCCHHHHCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHC
HRHFQPVIEAIIRLAEKAAKEPRPFEPVDHSALEAKIREIAEADLRAAYKIKQKQARYEA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGAAKSKVKKYFEQLAIDGQEAPKAQDVAEVLKGLEAKIVRWAILDDGIRIDGRDVKTVR
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHEEEEEECCCEEECCCCHHHHH
PIVSEVGILPRAHGSALFTRGETQALVVATLGTGEDEQFIDSLEGTYKEHFLLHYNFPPF
HHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCHHEEEEEECCCCC
SVGETGRMGSPGRREIGHGKLAWRAIHPMLPAKHEFPYTLRVVSEILESNGSSSMATVCG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHC
TSLSLMDAGVPLKSPVAGIAMGLILEGEKFAVLSDILGDEDHLGDMDFKVAGTESGVTAL
CCHHHHHCCCCCCCCHHHHHHHHEECCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCEEE
QMDIKIAGITEEIMKVALAQAKDGRAHILGEMSKALTSARAELGEHAPRIEVMKIAVDKI
EEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
REVIGSGGKVIREIVEKTGAKINIEDDGTIKIASASGDSIKAAINWIKSIASEPEVGAIY
HHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEE
DGTVVKVVDFGAFVNFFGSKDGLVHISQLSKERVAKSSDVVKEGDKVKVKLVGFDERGKT
CCCEEEEEHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
RLSMKAVDQTTGEDLEAKAKSEKDAAKEGDEAGAAE
EEEEHHHHCCCCCCHHHHHCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLPSAPCGGGAARASRRLAHGSPSSSEKKETFMFDIHREELDWGGRTLVLETGKMARQAD
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEEECCCHHHHCC
GAVFASYGDTTVLATVVSAREPKPGQDFFPLTVNYQEKTYAAGRIPGGYFKREGRPSEKE
CCEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEECCHHHEECCCCCCCHHCCCCCCCHHH
TLVSRLIDRPIRPLFADGYKNDTQVVVTVLAHDLENDPDVLSMVAASAALTLSGVPFMGP
HHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCCCCC
IGGARVGFVGDEYVLNPTIEELKDSALDLVVAGTSDAVLMVESEAKELSEEVMLGAVMFG
CCCCEEEECCCCEEECCCHHHHCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHC
HRHFQPVIEAIIRLAEKAAKEPRPFEPVDHSALEAKIREIAEADLRAAYKIKQKQARYEA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGAAKSKVKKYFEQLAIDGQEAPKAQDVAEVLKGLEAKIVRWAILDDGIRIDGRDVKTVR
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHEEEEEECCCEEECCCCHHHHH
PIVSEVGILPRAHGSALFTRGETQALVVATLGTGEDEQFIDSLEGTYKEHFLLHYNFPPF
HHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCHHEEEEEECCCCC
SVGETGRMGSPGRREIGHGKLAWRAIHPMLPAKHEFPYTLRVVSEILESNGSSSMATVCG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHC
TSLSLMDAGVPLKSPVAGIAMGLILEGEKFAVLSDILGDEDHLGDMDFKVAGTESGVTAL
CCHHHHHCCCCCCCCHHHHHHHHEECCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCEEE
QMDIKIAGITEEIMKVALAQAKDGRAHILGEMSKALTSARAELGEHAPRIEVMKIAVDKI
EEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
REVIGSGGKVIREIVEKTGAKINIEDDGTIKIASASGDSIKAAINWIKSIASEPEVGAIY
HHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEE
DGTVVKVVDFGAFVNFFGSKDGLVHISQLSKERVAKSSDVVKEGDKVKVKLVGFDERGKT
CCCEEEEEHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
RLSMKAVDQTTGEDLEAKAKSEKDAAKEGDEAGAAE
EEEEHHHHCCCCCCHHHHHCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA