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The map label for this gene is gloB

Identifier: 158338875

GI number: 158338875

Start: 5870820

End: 5871593

Strand: Reverse

Name: gloB

Synonym: AM1_5788

Alternate gene names: 158338875

Gene position: 5871593-5870820 (Counterclockwise)

Preceding gene: 158338879

Following gene: 158338871

Centisome position: 90.28

GC content: 50.78

Gene sequence:

>774_bases
ATGCAAATCCACCGACTACCGGCCTTTTCCGATAACTACATTTTTGTCCTCCATGATCCTGGGCAGAATATTGCCGCCGT
GGTTGATCCAGCCGATCCTCAGCCCGTACTGAAGAAATTGGCAGAGTTGGGGGCTGAGCTGGTCGCCATCTTCAATACCC
ATCACCATTCGGATCATGTGGGGGGCAATCGAACCCTCCTCCAGGCTTTTCCCAACACCGTTGTTTATGGAGGAGAGCAG
GATCGAGGTCGGATTCCCGGCCAGCAACACTTTCTAAAAGAAGGCGATCAAGTCTCTTTTGCTCATCGCAGGGCTGAGGT
TTACTTTGTTCCCGGCCATACCCGAGCCCATATTGCCTATTACTTCCCCCCTACGACAGGAGAAGAATGGGGGGAACTGT
TTTGTGGCGATACCTTGTTCGCAGGGGGATGTGGACGTTTGTTTGAGGGCACTCCGGCCCAGATGGTGGACTCTTTAAGC
AAGCTGCGCAACTTACCGGAGACCACCCGAGTCTGGTGTGCCCACGAATATACCCTCAAAAATTTGCAGTTTGCCCTGAC
GGTAGATTCAGACAATTCACACCTAAAAAATCGCTTTAAACAGGTGCAAAACGCTCGGAATCTATCCCAACCGACTGTTC
CCTCTGATATTGGCTTGGAAAAACAGACCAACCCCTTTCTGCGGTGGGATCAGCCCCATTTAATTATCCATACCAAAAGC
AATACCCCGGTCCAGTGTTTTGCACGGCTACGGGGTATGAAAGATCAGTTTTAA

Upstream 100 bases:

>100_bases
GTCGCAAGGTGGCTGATGTCAGCAGTGCAACCCGGCTTTCAACAACGGTTGGAGTCGCAAAATAATAAGATATCAGTGAT
ATAGAATTTTGCGATCGCCC

Downstream 100 bases:

>100_bases
TAGGTGTAATAGAGAAACTAGCTGAGGGCATTCAGGCCGCAGGCCGCCACCGCTAAAACGGCAACGCAAGCGTAAAAGGT
GGATACAACCCGGACTTCGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MQIHRLPAFSDNYIFVLHDPGQNIAAVVDPADPQPVLKKLAELGAELVAIFNTHHHSDHVGGNRTLLQAFPNTVVYGGEQ
DRGRIPGQQHFLKEGDQVSFAHRRAEVYFVPGHTRAHIAYYFPPTTGEEWGELFCGDTLFAGGCGRLFEGTPAQMVDSLS
KLRNLPETTRVWCAHEYTLKNLQFALTVDSDNSHLKNRFKQVQNARNLSQPTVPSDIGLEKQTNPFLRWDQPHLIIHTKS
NTPVQCFARLRGMKDQF

Sequences:

>Translated_257_residues
MQIHRLPAFSDNYIFVLHDPGQNIAAVVDPADPQPVLKKLAELGAELVAIFNTHHHSDHVGGNRTLLQAFPNTVVYGGEQ
DRGRIPGQQHFLKEGDQVSFAHRRAEVYFVPGHTRAHIAYYFPPTTGEEWGELFCGDTLFAGGCGRLFEGTPAQMVDSLS
KLRNLPETTRVWCAHEYTLKNLQFALTVDSDNSHLKNRFKQVQNARNLSQPTVPSDIGLEKQTNPFLRWDQPHLIIHTKS
NTPVQCFARLRGMKDQF
>Mature_257_residues
MQIHRLPAFSDNYIFVLHDPGQNIAAVVDPADPQPVLKKLAELGAELVAIFNTHHHSDHVGGNRTLLQAFPNTVVYGGEQ
DRGRIPGQQHFLKEGDQVSFAHRRAEVYFVPGHTRAHIAYYFPPTTGEEWGELFCGDTLFAGGCGRLFEGTPAQMVDSLS
KLRNLPETTRVWCAHEYTLKNLQFALTVDSDNSHLKNRFKQVQNARNLSQPTVPSDIGLEKQTNPFLRWDQPHLIIHTKS
NTPVQCFARLRGMKDQF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:
GO:0004416: Hydroxyacylglutathione hydrolase
GO:0008270: Hydroxyacylglutathione hydrolase
GO:0016787: Hydroxyacylglutathione hydrolase
GO:0046872: Hydroxyacylglutathione hydrolase

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538322, Length=258, Percent_Identity=35.6589147286822, Blast_Score=177, Evalue=9e-45,
Organism=Homo sapiens, GI94538320, Length=258, Percent_Identity=35.6589147286822, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI21703352, Length=223, Percent_Identity=39.0134529147982, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI116642887, Length=223, Percent_Identity=39.0134529147982, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI14150041, Length=257, Percent_Identity=32.6848249027237, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI46361987, Length=210, Percent_Identity=34.2857142857143, Blast_Score=132, Evalue=2e-31,
Organism=Escherichia coli, GI1786406, Length=261, Percent_Identity=42.5287356321839, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17536925, Length=253, Percent_Identity=35.5731225296443, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17538952, Length=186, Percent_Identity=27.4193548387097, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=267, Percent_Identity=30.7116104868914, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=234, Percent_Identity=32.9059829059829, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=40.7692307692308, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=40.7692307692308, Blast_Score=196, Evalue=9e-51,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=40.7692307692308, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI221330176, Length=177, Percent_Identity=31.638418079096, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_ACAM1 (B0BZI8)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001520052.1
- ProteinModelPortal:   B0BZI8
- SMR:   B0BZI8
- GeneID:   5684575
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5788
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- BioCyc:   AMAR329726:AM1_5788-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 29006; Mature: 29006

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIHRLPAFSDNYIFVLHDPGQNIAAVVDPADPQPVLKKLAELGAELVAIFNTHHHSDHV
CCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGNRTLLQAFPNTVVYGGEQDRGRIPGQQHFLKEGDQVSFAHRRAEVYFVPGHTRAHIAY
CCCCHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCEEEEECCEEEEEECCCCEEEEEE
YFPPTTGEEWGELFCGDTLFAGGCGRLFEGTPAQMVDSLSKLRNLPETTRVWCAHEYTLK
EECCCCCHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEEEEEEEC
NLQFALTVDSDNSHLKNRFKQVQNARNLSQPTVPSDIGLEKQTNPFLRWDQPHLIIHTKS
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECC
NTPVQCFARLRGMKDQF
CCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIHRLPAFSDNYIFVLHDPGQNIAAVVDPADPQPVLKKLAELGAELVAIFNTHHHSDHV
CCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGNRTLLQAFPNTVVYGGEQDRGRIPGQQHFLKEGDQVSFAHRRAEVYFVPGHTRAHIAY
CCCCHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCEEEEECCEEEEEECCCCEEEEEE
YFPPTTGEEWGELFCGDTLFAGGCGRLFEGTPAQMVDSLSKLRNLPETTRVWCAHEYTLK
EECCCCCHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEEEEEEEC
NLQFALTVDSDNSHLKNRFKQVQNARNLSQPTVPSDIGLEKQTNPFLRWDQPHLIIHTKS
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECC
NTPVQCFARLRGMKDQF
CCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA