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The map label for this gene is purH

Identifier: 158338866

GI number: 158338866

Start: 5859814

End: 5861388

Strand: Direct

Name: purH

Synonym: AM1_5779

Alternate gene names: 158338866

Gene position: 5859814-5861388 (Clockwise)

Preceding gene: 158338865

Following gene: 158338868

Centisome position: 90.1

GC content: 51.56

Gene sequence:

>1575_bases
ATGACGCGTTTAGCCCTCTTAAGTACCTCAGATAAAACTGGGTTGGTTGAGTTAGCCCAGCAGTTAGTCAATGAATATGG
TTTTACCCTGGTAAGTAGTGGAGGGACTGCGGTAACCATAGAAAAAGCTGGATTGCCTGTCACTAAGGTGTCAGACTATA
CCGGGTCCCCGGAAATTTTAGGCGGACGAGTCAAGACTCTTCATCCTCGCATTCATGGTGGGATTTTGGCTCGTCGAAGT
TTAGAGTCTGATGTACAGGACCTAACCGAGAACAATATCCAGGGGATTGATTTAGTGGTGGTGAACCTATATCCCTTTGA
AGAGACCATTGCCAAACAAAAAGCTCAGGGAATCACAGATCCAGACCAAGCATTGGCAGATGCAGTCGAACAAATTGATA
TCGGCGGCCCCACCATGATTCGGGCAGCTGCCAAAAATCATGCCCATGTGACCGTGTTATGTGACCCTCAGCAGTATGCG
TCTTATTTACAAGAACTGAAACACGGGGAAGGAGAGACGTCGCTGCCATTTCGACAGGCTTGCGCCCTTAAGGTGTTTGA
GCGAATGGCGAGTTATGACCGTGCGATCGCAACCCACCTCCGCCAATCCCTAGCTACTACCGATTCCCAAACCCAGCTCT
CCATCTTGGGTACAGCCAAACAAACCCTTCGCTATGGTGAAAATCCCCACCAAAATGCCGTTTGGTATCAAGAAAGCGAT
ACGGCTTCGGGATGGGCTGCTGCTCAACAGCTCCAAGGAAAGGAACTCAGCTACAACAATCTGGTTGATTTAGAAGCTGC
TCGGCGTGTAATCGCTGAATTTGCTCAGGCCGAAACCCAACCCACGGTAGCGATTCTCAAACACAACAACCCTTGCGGCG
TCGCCCAAGGGGAAACCCTGCTCCAGGCTTATGAAAAAGCCCTAGCCGCCGATTCAGTTTCTGCTTTTGGCGGTATCGTG
GCTATGAATCGTGCCATTGATACCGATACGGCCCAGGTGCTCACCAAAACCTTTTTGGAGTGTATTGTTGCCCCTGAGTG
TCAACCTGAGGCTGCGGCTGTCCTCCAGGCCAAGTCGAACTTACGGGTGTTGGTGCTACCGGATTTGGTCAGTGGTCCCG
CGGTTACGGTAAAAGCGATCGCAGGGGGGTGGCTGGCCCAAGCCGCTGATGAAACCTTAACGCCCCCTGCTGATTGGCAG
ATTGTTACCCAAGCGAAGCCTACTGAGGCTCAGTTAGCGGAACTTCTGTTTGCCTGGAAAGTGGTCAAACATGTCAAGTC
CAATGCCATTGTGGTGACCAAAGATCACACCACGTTGGGGGTTGGTGCAGGCCAAATGAACCGAGTGGGCTCGGTTAATA
TTGCTCTGCAGCAGGCAGGGGAAAAAGCTCAAGGTGGAACCCTAGCTAGCGACGGTTTCTTCCCCTTTGATGACTCGGTC
CGCACAGCCGCCGCTGCCGGAATTACGGCTATCATTCAGCCTGGTGGCAGTATTCGAGACAAAGATTCCATTCAAGCTGC
CGATGAACTGGGAATTGTGATGGCCTTTACGGGCACCCGTCATTTTCTCCACTAA

Upstream 100 bases:

>100_bases
ATGAAGGGAAATAGTGGCAGCTTCTTGCAGTAAATTCAAAATCCAATACAGCTTTCTCTTCAATCTGCTGTCAACAATGT
AGTGTTTTCAGAATTTGGTA

Downstream 100 bases:

>100_bases
TCCCTTCGGGAATCAGACCGTTTGGAGTGATTGCTGCGATCGCAGTCCCTTTTTCTGAATCTGAACAGATTGTTTTTGCC
CTAAAGTAACCCCTCGACGT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTRLALLSTSDKTGLVELAQQLVNEYGFTLVSSGGTAVTIEKAGLPVTKVSDYTGSPEILGGRVKTLHPRIHGGILARRS
LESDVQDLTENNIQGIDLVVVNLYPFEETIAKQKAQGITDPDQALADAVEQIDIGGPTMIRAAAKNHAHVTVLCDPQQYA
SYLQELKHGEGETSLPFRQACALKVFERMASYDRAIATHLRQSLATTDSQTQLSILGTAKQTLRYGENPHQNAVWYQESD
TASGWAAAQQLQGKELSYNNLVDLEAARRVIAEFAQAETQPTVAILKHNNPCGVAQGETLLQAYEKALAADSVSAFGGIV
AMNRAIDTDTAQVLTKTFLECIVAPECQPEAAAVLQAKSNLRVLVLPDLVSGPAVTVKAIAGGWLAQAADETLTPPADWQ
IVTQAKPTEAQLAELLFAWKVVKHVKSNAIVVTKDHTTLGVGAGQMNRVGSVNIALQQAGEKAQGGTLASDGFFPFDDSV
RTAAAAGITAIIQPGGSIRDKDSIQAADELGIVMAFTGTRHFLH

Sequences:

>Translated_524_residues
MTRLALLSTSDKTGLVELAQQLVNEYGFTLVSSGGTAVTIEKAGLPVTKVSDYTGSPEILGGRVKTLHPRIHGGILARRS
LESDVQDLTENNIQGIDLVVVNLYPFEETIAKQKAQGITDPDQALADAVEQIDIGGPTMIRAAAKNHAHVTVLCDPQQYA
SYLQELKHGEGETSLPFRQACALKVFERMASYDRAIATHLRQSLATTDSQTQLSILGTAKQTLRYGENPHQNAVWYQESD
TASGWAAAQQLQGKELSYNNLVDLEAARRVIAEFAQAETQPTVAILKHNNPCGVAQGETLLQAYEKALAADSVSAFGGIV
AMNRAIDTDTAQVLTKTFLECIVAPECQPEAAAVLQAKSNLRVLVLPDLVSGPAVTVKAIAGGWLAQAADETLTPPADWQ
IVTQAKPTEAQLAELLFAWKVVKHVKSNAIVVTKDHTTLGVGAGQMNRVGSVNIALQQAGEKAQGGTLASDGFFPFDDSV
RTAAAAGITAIIQPGGSIRDKDSIQAADELGIVMAFTGTRHFLH
>Mature_523_residues
TRLALLSTSDKTGLVELAQQLVNEYGFTLVSSGGTAVTIEKAGLPVTKVSDYTGSPEILGGRVKTLHPRIHGGILARRSL
ESDVQDLTENNIQGIDLVVVNLYPFEETIAKQKAQGITDPDQALADAVEQIDIGGPTMIRAAAKNHAHVTVLCDPQQYAS
YLQELKHGEGETSLPFRQACALKVFERMASYDRAIATHLRQSLATTDSQTQLSILGTAKQTLRYGENPHQNAVWYQESDT
ASGWAAAQQLQGKELSYNNLVDLEAARRVIAEFAQAETQPTVAILKHNNPCGVAQGETLLQAYEKALAADSVSAFGGIVA
MNRAIDTDTAQVLTKTFLECIVAPECQPEAAAVLQAKSNLRVLVLPDLVSGPAVTVKAIAGGWLAQAADETLTPPADWQI
VTQAKPTEAQLAELLFAWKVVKHVKSNAIVVTKDHTTLGVGAGQMNRVGSVNIALQQAGEKAQGGTLASDGFFPFDDSVR
TAAAAGITAIIQPGGSIRDKDSIQAADELGIVMAFTGTRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:
GO:0003824: Bifunctional purine biosynthesis protein purH
GO:0003937: Bifunctional purine biosynthesis protein purH
GO:0004643: Bifunctional purine biosynthesis protein purH
GO:0006164: Bifunctional purine biosynthesis protein purH
GO:0008152: Bifunctional purine biosynthesis protein purH
GO:0016740: Bifunctional purine biosynthesis protein purH
GO:0016787: Bifunctional purine biosynthesis protein purH

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=488, Percent_Identity=36.8852459016393, Blast_Score=253, Evalue=4e-67,
Organism=Escherichia coli, GI1790439, Length=538, Percent_Identity=46.0966542750929, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71985564, Length=492, Percent_Identity=35.5691056910569, Blast_Score=243, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71985574, Length=317, Percent_Identity=26.1829652996845, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=491, Percent_Identity=37.6782077393075, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6323056, Length=483, Percent_Identity=37.4741200828157, Blast_Score=275, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24649832, Length=490, Percent_Identity=36.9387755102041, Blast_Score=268, Evalue=6e-72,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_ACAM1 (B0BZH9)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001520043.1
- ProteinModelPortal:   B0BZH9
- SMR:   B0BZH9
- GeneID:   5684566
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5779
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   AMAR329726:AM1_5779-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55849; Mature: 55718

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLALLSTSDKTGLVELAQQLVNEYGFTLVSSGGTAVTIEKAGLPVTKVSDYTGSPEIL
CCEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCCCCCCHHC
GGRVKTLHPRIHGGILARRSLESDVQDLTENNIQGIDLVVVNLYPFEETIAKQKAQGITD
CCCCEEECCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHCCCCC
PDQALADAVEQIDIGGPTMIRAAAKNHAHVTVLCDPQQYASYLQELKHGEGETSLPFRQA
HHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCHHHH
CALKVFERMASYDRAIATHLRQSLATTDSQTQLSILGTAKQTLRYGENPHQNAVWYQESD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCCCCEEEECCC
TASGWAAAQQLQGKELSYNNLVDLEAARRVIAEFAQAETQPTVAILKHNNPCGVAQGETL
CCCHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
LQAYEKALAADSVSAFGGIVAMNRAIDTDTAQVLTKTFLECIVAPECQPEAAAVLQAKSN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
LRVLVLPDLVSGPAVTVKAIAGGWLAQAADETLTPPADWQIVTQAKPTEAQLAELLFAWK
CEEEEECCCCCCCCEEEEEHHCCHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
VVKHVKSNAIVVTKDHTTLGVGAGQMNRVGSVNIALQQAGEKAQGGTLASDGFFPFDDSV
HHHHHCCCEEEEEECCCEEEECCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCH
RTAAAAGITAIIQPGGSIRDKDSIQAADELGIVMAFTGTRHFLH
HHHHHHCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCHHCCC
>Mature Secondary Structure 
TRLALLSTSDKTGLVELAQQLVNEYGFTLVSSGGTAVTIEKAGLPVTKVSDYTGSPEIL
CEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCCCCCCHHC
GGRVKTLHPRIHGGILARRSLESDVQDLTENNIQGIDLVVVNLYPFEETIAKQKAQGITD
CCCCEEECCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHCCCCC
PDQALADAVEQIDIGGPTMIRAAAKNHAHVTVLCDPQQYASYLQELKHGEGETSLPFRQA
HHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCHHHH
CALKVFERMASYDRAIATHLRQSLATTDSQTQLSILGTAKQTLRYGENPHQNAVWYQESD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCCCCEEEECCC
TASGWAAAQQLQGKELSYNNLVDLEAARRVIAEFAQAETQPTVAILKHNNPCGVAQGETL
CCCHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
LQAYEKALAADSVSAFGGIVAMNRAIDTDTAQVLTKTFLECIVAPECQPEAAAVLQAKSN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
LRVLVLPDLVSGPAVTVKAIAGGWLAQAADETLTPPADWQIVTQAKPTEAQLAELLFAWK
CEEEEECCCCCCCCEEEEEHHCCHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
VVKHVKSNAIVVTKDHTTLGVGAGQMNRVGSVNIALQQAGEKAQGGTLASDGFFPFDDSV
HHHHHCCCEEEEEECCCEEEECCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCH
RTAAAAGITAIIQPGGSIRDKDSIQAADELGIVMAFTGTRHFLH
HHHHHHCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA