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The map label for this gene is anfA [H]

Identifier: 158338756

GI number: 158338756

Start: 5730109

End: 5732637

Strand: Direct

Name: anfA [H]

Synonym: AM1_5667

Alternate gene names: 158338756

Gene position: 5730109-5732637 (Clockwise)

Preceding gene: 158338754

Following gene: 158338757

Centisome position: 88.11

GC content: 49.55

Gene sequence:

>2529_bases
ATGGCATCCTCAGAGGACAAAAAGGCTTGGTTACAGGAGCATACTGATTTAAATATCTTTCCCGAAAAGATCTTGGATCG
GCTGGCGAGAAAGGTAAAAGTACAGACCTTGGAAGCCAATCGGCGGTTGATTCTAGAGGATACGTTGCCGGATGCTGTAT
ATATTTTGCGTCAAGGCCATCTTGAAAGTTATCACACCAGTTTGGTGGATACGGCCCAGGTCTGTAGCTTGTTACCCGGG
GCAGTGCTGCATCTATTCGATATCTTGCTCGAACAGCCGACGCTTCAAACCATCATTACTTTAACGGATTGCGAATTTTG
GGTCATTTCTAAAAAAGACCTGCTCAAGATCGCCGGTGAATATCAAGACGTGACCCGCATCGTGTCGCAAAAGCTATCAA
AAGATTTGGCTCATGTTTCACGAGAACTGGAGGCTGAACAGGAGCGACAAGCCATCCTCAGACCCTATTTGGTCCCTAGA
GTAAATCGGGGAATCGTCGGTTCTAGCCGCTATGCCAAGCGGTTGCGAGAACAACTCAAAGAGGCAGCCTGCACCCGCCA
TTCAGTGATTATCTTTGGTGAACCCGGTCTAGAGAAAGATAATGCTGCTGCTTTGATTCACTTCGGCTCGAACCAGCGGC
GCGAGCCGATGGTGAAAATTAACTGCAATACGCTTCAAATTAGCGGGGCCGATTTGTTTGGTCGAGTGGGGGGTAAGCCT
GGGCTGCTAGAAGCGGTGGGTGAGGGGACTGTTCTTCTCAATAATATTCAAGATCTGCCGCCCGTACTATGTCCAAAGCT
ACGACAGCTGCTAGAAACCCAAACTTATTTACCGGTTCAAAGAGACGGGACTGCTGCTAAGCCCCGAACCAGCAACGCTC
GTTTAATCTTTACGGCTGAGAAAATTCAGCCGGAACTGGATGGGGAGATTTTCCATCGGATTAAGATGCCGCCGTTGCGG
GTCAGTAAGGCGGATGTGGAGGCTCAGGTTGAGTACTATATCAGTCTGCTTTCTAAAGAACAGGGATTTGCTAAGCGCCA
GTTGACCCCTGAAGCCTTACGACGACTGCAAAGCTACAATTATCCGGGCAATATTGTCGAGCTTCATAGCTTGGTGGAGC
GTGCGATCGCACAATCGTCTCCCAACGAAGATCTCACCGCAGAAATCTTTTGGGCAGCCCAAGATCGCCAACAGCGCTTC
CGTCTTAACCTGCTGAATGCCAACCCTTGGTTGCGCCGTTTTCTCCGCAGTCCCTGGTGGCCCACCCGCATTAACTATGG
AATAGTCCTACCCCTGTTTATTTTTGTGGTGATTATTCTATTTGTGGGTCCTCAGCAGCGGAGTCAGAACTTTGCCTTGA
ACTTGTTTTGGGCTTGGTGGTGGCCGTTGATATTAATTGGCTTTCCCTTTGTGGGCCGTCTCTGGTGCTCCGTCTGTCCC
TTTATGATTTGTGGTGAACTGCTGCAAAAACTGTCTTTATGGCTGGTGCCTAGACAACTGCAGCGCTGGCCCCGGCAGCA
GGCGGAGCAGTGGGGAGGCTGGTTCTTGTTTGGTCTGTTTGCTCTGATTTTTCTCTGGGAGGAGCTGTGGAATCTAGAGG
ATACGGCTTATCTGTCTAGTTGTCTGCTGTTGTTGATTACGGCGGGGGCAGTGATTTGTTCGCAGATTTTTGAGCGACGA
TTTTGGTGTCGATATCTTTGTCCCATCGGGGGAATGAACGGGTTATTTGCCAAGCTGTCCATGACAGAGCTAAGGGCGCA
ACAGGGCACCTGTTCTGCTGAGTGCAGTACCTACCAATGTTATAAAGGTGGACCTGAAAAAGGGGAAGGACAAGAAACAG
ATGGTTGCCCTCTCTACTCCCACCCTGCCCAGTTGGAAGATAACCGAGACTGTGTGCTCTGTATGACCTGCTTGCAGGCC
TGCCCTCACCGCTCTGTTGAGTTCAATTTGCGTCCGCCCGGCATTGAATTATGGACGACCCATACCCCTCGGATCTATGA
GGTTGCACTGCTCCTACTGTTGCTAGGAGGGATATTCCTGCATCGATTACCCGAGCTGCAAGCCTGGTTGGGGTTAGATT
TTCACTTACAGCAGTTTTCAGCTCATTTACTGGTGTCGGTGTTGGTGTTGGGAATCGCTGCCTTGATTCCCTTGCTTACC
CATACGATCCTATGTCGCATCAGTACTAAATTAAAACCTCGACCCTTTATCGAATTGGTCTATGGCTATTTGCCTTTAGT
TCTGGGTGGTACCTTAGCCCACTATTTAAGGTTAGGACTCACAGAAGCAGGCCAAATTCTTCCGGTCACCTGGGTAACGT
TTGGTTTCTCTGGCGTAGGTTTACCCATCTGGGTGGCCCATCCTGCGGTAGTTAGTTTCCTTCAGGGTTCTACGATATTG
ACGGCAATTTTTTTGACATGGGTGTTGACTCAAAAAATTGCCCGACAACCCATTACTGTTCTGCTGCCCCAGCATATGAC
CAGCCTGGGACTAGGATTCTGTTTATGGTCAGTGATTGTTGGGGTTTAA

Upstream 100 bases:

>100_bases
TCCCTAATCTTGAGCGGCGGTTTGAGATTGGAACCACTGGTTTGAAGGGAATGAAAATACAGCCTTTCTGACCACACCTA
CATTGAGGAGAAAAATTCCC

Downstream 100 bases:

>100_bases
TTACTAACGGTAGGTAATCTAGAGACTAACTTGGGCAGTAATTTGAGTCTTGATTCGAAGCATTAAGCCTCAATAAAAAA
TCGATCAGTTTGGGTCGTAG

Product: nitrogen regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 842; Mature: 841

Protein sequence:

>842_residues
MASSEDKKAWLQEHTDLNIFPEKILDRLARKVKVQTLEANRRLILEDTLPDAVYILRQGHLESYHTSLVDTAQVCSLLPG
AVLHLFDILLEQPTLQTIITLTDCEFWVISKKDLLKIAGEYQDVTRIVSQKLSKDLAHVSRELEAEQERQAILRPYLVPR
VNRGIVGSSRYAKRLREQLKEAACTRHSVIIFGEPGLEKDNAAALIHFGSNQRREPMVKINCNTLQISGADLFGRVGGKP
GLLEAVGEGTVLLNNIQDLPPVLCPKLRQLLETQTYLPVQRDGTAAKPRTSNARLIFTAEKIQPELDGEIFHRIKMPPLR
VSKADVEAQVEYYISLLSKEQGFAKRQLTPEALRRLQSYNYPGNIVELHSLVERAIAQSSPNEDLTAEIFWAAQDRQQRF
RLNLLNANPWLRRFLRSPWWPTRINYGIVLPLFIFVVIILFVGPQQRSQNFALNLFWAWWWPLILIGFPFVGRLWCSVCP
FMICGELLQKLSLWLVPRQLQRWPRQQAEQWGGWFLFGLFALIFLWEELWNLEDTAYLSSCLLLLITAGAVICSQIFERR
FWCRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQCYKGGPEKGEGQETDGCPLYSHPAQLEDNRDCVLCMTCLQA
CPHRSVEFNLRPPGIELWTTHTPRIYEVALLLLLLGGIFLHRLPELQAWLGLDFHLQQFSAHLLVSVLVLGIAALIPLLT
HTILCRISTKLKPRPFIELVYGYLPLVLGGTLAHYLRLGLTEAGQILPVTWVTFGFSGVGLPIWVAHPAVVSFLQGSTIL
TAIFLTWVLTQKIARQPITVLLPQHMTSLGLGFCLWSVIVGV

Sequences:

>Translated_842_residues
MASSEDKKAWLQEHTDLNIFPEKILDRLARKVKVQTLEANRRLILEDTLPDAVYILRQGHLESYHTSLVDTAQVCSLLPG
AVLHLFDILLEQPTLQTIITLTDCEFWVISKKDLLKIAGEYQDVTRIVSQKLSKDLAHVSRELEAEQERQAILRPYLVPR
VNRGIVGSSRYAKRLREQLKEAACTRHSVIIFGEPGLEKDNAAALIHFGSNQRREPMVKINCNTLQISGADLFGRVGGKP
GLLEAVGEGTVLLNNIQDLPPVLCPKLRQLLETQTYLPVQRDGTAAKPRTSNARLIFTAEKIQPELDGEIFHRIKMPPLR
VSKADVEAQVEYYISLLSKEQGFAKRQLTPEALRRLQSYNYPGNIVELHSLVERAIAQSSPNEDLTAEIFWAAQDRQQRF
RLNLLNANPWLRRFLRSPWWPTRINYGIVLPLFIFVVIILFVGPQQRSQNFALNLFWAWWWPLILIGFPFVGRLWCSVCP
FMICGELLQKLSLWLVPRQLQRWPRQQAEQWGGWFLFGLFALIFLWEELWNLEDTAYLSSCLLLLITAGAVICSQIFERR
FWCRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQCYKGGPEKGEGQETDGCPLYSHPAQLEDNRDCVLCMTCLQA
CPHRSVEFNLRPPGIELWTTHTPRIYEVALLLLLLGGIFLHRLPELQAWLGLDFHLQQFSAHLLVSVLVLGIAALIPLLT
HTILCRISTKLKPRPFIELVYGYLPLVLGGTLAHYLRLGLTEAGQILPVTWVTFGFSGVGLPIWVAHPAVVSFLQGSTIL
TAIFLTWVLTQKIARQPITVLLPQHMTSLGLGFCLWSVIVGV
>Mature_841_residues
ASSEDKKAWLQEHTDLNIFPEKILDRLARKVKVQTLEANRRLILEDTLPDAVYILRQGHLESYHTSLVDTAQVCSLLPGA
VLHLFDILLEQPTLQTIITLTDCEFWVISKKDLLKIAGEYQDVTRIVSQKLSKDLAHVSRELEAEQERQAILRPYLVPRV
NRGIVGSSRYAKRLREQLKEAACTRHSVIIFGEPGLEKDNAAALIHFGSNQRREPMVKINCNTLQISGADLFGRVGGKPG
LLEAVGEGTVLLNNIQDLPPVLCPKLRQLLETQTYLPVQRDGTAAKPRTSNARLIFTAEKIQPELDGEIFHRIKMPPLRV
SKADVEAQVEYYISLLSKEQGFAKRQLTPEALRRLQSYNYPGNIVELHSLVERAIAQSSPNEDLTAEIFWAAQDRQQRFR
LNLLNANPWLRRFLRSPWWPTRINYGIVLPLFIFVVIILFVGPQQRSQNFALNLFWAWWWPLILIGFPFVGRLWCSVCPF
MICGELLQKLSLWLVPRQLQRWPRQQAEQWGGWFLFGLFALIFLWEELWNLEDTAYLSSCLLLLITAGAVICSQIFERRF
WCRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQCYKGGPEKGEGQETDGCPLYSHPAQLEDNRDCVLCMTCLQAC
PHRSVEFNLRPPGIELWTTHTPRIYEVALLLLLLGGIFLHRLPELQAWLGLDFHLQQFSAHLLVSVLVLGIAALIPLLTH
TILCRISTKLKPRPFIELVYGYLPLVLGGTLAHYLRLGLTEAGQILPVTWVTFGFSGVGLPIWVAHPAVVSFLQGSTILT
AIFLTWVLTQKIARQPITVLLPQHMTSLGLGFCLWSVIVGV

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG0348

COG function: function code C; Polyferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=231, Percent_Identity=29.4372294372294, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI87082117, Length=279, Percent_Identity=28.3154121863799, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788550, Length=231, Percent_Identity=29.004329004329, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=28.8793103448276, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1789087, Length=354, Percent_Identity=25.4237288135593, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790437, Length=234, Percent_Identity=27.3504273504274, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI87081872, Length=227, Percent_Identity=28.1938325991189, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787583, Length=228, Percent_Identity=27.1929824561404, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1788905, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786524, Length=258, Percent_Identity=29.0697674418605, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1790299, Length=229, Percent_Identity=27.0742358078603, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 95510; Mature: 95379

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSEDKKAWLQEHTDLNIFPEKILDRLARKVKVQTLEANRRLILEDTLPDAVYILRQGH
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHHCC
LESYHTSLVDTAQVCSLLPGAVLHLFDILLEQPTLQTIITLTDCEFWVISKKDLLKIAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEECHHHHHHHHCC
YQDVTRIVSQKLSKDLAHVSRELEAEQERQAILRPYLVPRVNRGIVGSSRYAKRLREQLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EAACTRHSVIIFGEPGLEKDNAAALIHFGSNQRREPMVKINCNTLQISGADLFGRVGGKP
HHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEECCEEEEECHHHHHHCCCCC
GLLEAVGEGTVLLNNIQDLPPVLCPKLRQLLETQTYLPVQRDGTAAKPRTSNARLIFTAE
CHHHHCCCCCEEECCHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEHH
KIQPELDGEIFHRIKMPPLRVSKADVEAQVEYYISLLSKEQGFAKRQLTPEALRRLQSYN
HCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHCCC
YPGNIVELHSLVERAIAQSSPNEDLTAEIFWAAQDRQQRFRLNLLNANPWLRRFLRSPWW
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHEEEEECCCHHHHHHHHCCCC
PTRINYGIVLPLFIFVVIILFVGPQQRSQNFALNLFWAWWWPLILIGFPFVGRLWCSVCP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
FMICGELLQKLSLWLVPRQLQRWPRQQAEQWGGWFLFGLFALIFLWEELWNLEDTAYLSS
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
CLLLLITAGAVICSQIFERRFWCRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQC
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEC
YKGGPEKGEGQETDGCPLYSHPAQLEDNRDCVLCMTCLQACPHRSVEFNLRPPGIELWTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEC
HTPRIYEVALLLLLLGGIFLHRLPELQAWLGLDFHLQQFSAHLLVSVLVLGIAALIPLLT
CCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTILCRISTKLKPRPFIELVYGYLPLVLGGTLAHYLRLGLTEAGQILPVTWVTFGFSGVG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCCCC
LPIWVAHPAVVSFLQGSTILTAIFLTWVLTQKIARQPITVLLPQHMTSLGLGFCLWSVIV
CEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHH
GV
CC
>Mature Secondary Structure 
ASSEDKKAWLQEHTDLNIFPEKILDRLARKVKVQTLEANRRLILEDTLPDAVYILRQGH
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHHCC
LESYHTSLVDTAQVCSLLPGAVLHLFDILLEQPTLQTIITLTDCEFWVISKKDLLKIAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEECHHHHHHHHCC
YQDVTRIVSQKLSKDLAHVSRELEAEQERQAILRPYLVPRVNRGIVGSSRYAKRLREQLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EAACTRHSVIIFGEPGLEKDNAAALIHFGSNQRREPMVKINCNTLQISGADLFGRVGGKP
HHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEECCEEEEECHHHHHHCCCCC
GLLEAVGEGTVLLNNIQDLPPVLCPKLRQLLETQTYLPVQRDGTAAKPRTSNARLIFTAE
CHHHHCCCCCEEECCHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEHH
KIQPELDGEIFHRIKMPPLRVSKADVEAQVEYYISLLSKEQGFAKRQLTPEALRRLQSYN
HCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHCCC
YPGNIVELHSLVERAIAQSSPNEDLTAEIFWAAQDRQQRFRLNLLNANPWLRRFLRSPWW
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHEEEEECCCHHHHHHHHCCCC
PTRINYGIVLPLFIFVVIILFVGPQQRSQNFALNLFWAWWWPLILIGFPFVGRLWCSVCP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
FMICGELLQKLSLWLVPRQLQRWPRQQAEQWGGWFLFGLFALIFLWEELWNLEDTAYLSS
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
CLLLLITAGAVICSQIFERRFWCRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQC
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEC
YKGGPEKGEGQETDGCPLYSHPAQLEDNRDCVLCMTCLQACPHRSVEFNLRPPGIELWTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEC
HTPRIYEVALLLLLLGGIFLHRLPELQAWLGLDFHLQQFSAHLLVSVLVLGIAALIPLLT
CCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTILCRISTKLKPRPFIELVYGYLPLVLGGTLAHYLRLGLTEAGQILPVTWVTFGFSGVG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCCCC
LPIWVAHPAVVSFLQGSTILTAIFLTWVLTQKIARQPITVLLPQHMTSLGLGFCLWSVIV
CEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHH
GV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]