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The map label for this gene is yfgF [H]

Identifier: 158338715

GI number: 158338715

Start: 5682970

End: 5684637

Strand: Direct

Name: yfgF [H]

Synonym: AM1_5623

Alternate gene names: 158338715

Gene position: 5682970-5684637 (Clockwise)

Preceding gene: 158338713

Following gene: 158338716

Centisome position: 87.38

GC content: 46.76

Gene sequence:

>1668_bases
TTGTCTGATGGCATTAATCTTCTCAATCACAGAGAGGGAATTGACTTAATCCTGTTGGATTTATGTTTATCAGATGCTCA
AGGATTAGAGACGTTTTATCAAGTCCACCAAGCTTTTCCTGCCAAGCCTTTAATTATTTTAAGCGGCAATACCGATCAAT
CTTTGGTGGAAGAGGCTCTCCAAAAAGGAGCATTGGATTTTCTAGTGAAAGGGCAGTTTGATAGCACTTCCCTTTGCCGT
TCGATTCAATATGCCCTAAAACAGCAGCATCAATCTGTTGAAATTGGATATCGAGATCTCACCCTTGATGCCCTCACCCG
GCAGCTCAAAATTGCTCAACAGGAGCTAGAACGATTAGCGGAGGTGGACAAGGTTACCCGCGTCTTTAACCGCTGTCGGT
TTGATGAAGTGTACCTGTCGGAATGGAATCGCTTGACGCGAGAAGGCTCCCATTTATCCTTAGTCTTGTGTGAAATTGAT
AATCTACAAGCCTACCAAGAGAAATATGGGGCTGAAGTCTGCGATTACTGTTTGCAGCGGGTTGCTGAAGAGATCGTAAA
AGTTGCCAGACGCCCTGCTGATTGCGTGGCTCGTTATAGTGGGCGAACCTTTATGATTCTGTTGCCCAATACCGATTTGG
CCGGTGCCACTTATCTTGCAGAAGCGATTCGCACGGGGGTCAGGTCGGTGCCGTTCCCCCCTGAAAGTTTAGGGGTAACG
AATTCCCCGATCACCCTGAGTTTAGGGGTGGCTTGTCAAATTCCACGGGCAAAAGTTGCGCCGTCTTTGCTGATTGAAGC
GGTGCATCAAGCCCTCCATTTTGCGCGAGAACAAGGGAAAGATCGAGTCCAGATTCATCATACGGGGTGTGCAGACCCAG
ACCTCCACTCAAACCAGACCCTGCATTGGGTGAGTCGACTATTAAAGGCTTTAGATAAGGACCAATTTAAGCTTTATGCT
CAGCCCATTTACCCCCTGAGCCGGGGGCAAAAGTCCTGCAGTTATGAGATTTTATTGCGGTTACAGGATCAGCCTGGTAT
TGTTTGTTCCCCCAGTTTGTTTTTACCCATGGTGGAACAGTATGACTTTATGACTCGAATTGATCGGTGGGTGATTAACA
ACTTGCTGGCTAATTTGGAAACTATGCCTGCTTTGGATGAACTGGATGCCCGGTTCTTTATTCACTTGTCAAGCGCGACC
TGCCGCAATGGAGAACTGTTTGAGTTTTTGGATAAACAATTTTCTCACTCCAATCGTCATGTGCAGCGATTCTGCTTTGA
GGTGACTGAAAATGTGGCTCTGAGTCATTTGACGTCTACAGTGGAATTGAGCCGTTTTCTCAAATCTATGGGATGCCAGA
TTGCGCTAGATAATTTTGGTAGTGATATGTCTGCCTTTATGAATTTGAAACATCTGCCCGTCGATTTTGTGAAAATCGAT
GGGTTGTTGGTCCGGGATATTGCCCAAGATCAAATTTCTCGGGGCATTGTTGAGTCGATTCATAATGTGGCCAAGGTGAT
GGGCATGCAGACGATTGCTGAGCATGTGGAGTCGGATGTCGTGCTGGATGCGATCGCAAATGTGGGTATTGATTATGCTC
AAGGGCATTACTATGATCGCGCCAAGCCTTTACTCGACGTAGTGTCAGCGACTCGCCGATCTCGCTAG

Upstream 100 bases:

>100_bases
AATCTTGATGATAGAGGATGATGATCAAGATGCGGAGCTTGTACGCAGATACTTGACAGTCAAGCAGGCTTTCCCGATTC
AGATTGAGCACAGCAAGACC

Downstream 100 bases:

>100_bases
AGCCGAGCGTATTGTTCAGACTTGTTCGATAGCTTCCCTTTAGCCCTTGAGTCTCGAACCCGATTTCTAAGATTTTCTGG
GATTTAAGGTTGTAGGCTAA

Product: PAS sensor diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSDGINLLNHREGIDLILLDLCLSDAQGLETFYQVHQAFPAKPLIILSGNTDQSLVEEALQKGALDFLVKGQFDSTSLCR
SIQYALKQQHQSVEIGYRDLTLDALTRQLKIAQQELERLAEVDKVTRVFNRCRFDEVYLSEWNRLTREGSHLSLVLCEID
NLQAYQEKYGAEVCDYCLQRVAEEIVKVARRPADCVARYSGRTFMILLPNTDLAGATYLAEAIRTGVRSVPFPPESLGVT
NSPITLSLGVACQIPRAKVAPSLLIEAVHQALHFAREQGKDRVQIHHTGCADPDLHSNQTLHWVSRLLKALDKDQFKLYA
QPIYPLSRGQKSCSYEILLRLQDQPGIVCSPSLFLPMVEQYDFMTRIDRWVINNLLANLETMPALDELDARFFIHLSSAT
CRNGELFEFLDKQFSHSNRHVQRFCFEVTENVALSHLTSTVELSRFLKSMGCQIALDNFGSDMSAFMNLKHLPVDFVKID
GLLVRDIAQDQISRGIVESIHNVAKVMGMQTIAEHVESDVVLDAIANVGIDYAQGHYYDRAKPLLDVVSATRRSR

Sequences:

>Translated_555_residues
MSDGINLLNHREGIDLILLDLCLSDAQGLETFYQVHQAFPAKPLIILSGNTDQSLVEEALQKGALDFLVKGQFDSTSLCR
SIQYALKQQHQSVEIGYRDLTLDALTRQLKIAQQELERLAEVDKVTRVFNRCRFDEVYLSEWNRLTREGSHLSLVLCEID
NLQAYQEKYGAEVCDYCLQRVAEEIVKVARRPADCVARYSGRTFMILLPNTDLAGATYLAEAIRTGVRSVPFPPESLGVT
NSPITLSLGVACQIPRAKVAPSLLIEAVHQALHFAREQGKDRVQIHHTGCADPDLHSNQTLHWVSRLLKALDKDQFKLYA
QPIYPLSRGQKSCSYEILLRLQDQPGIVCSPSLFLPMVEQYDFMTRIDRWVINNLLANLETMPALDELDARFFIHLSSAT
CRNGELFEFLDKQFSHSNRHVQRFCFEVTENVALSHLTSTVELSRFLKSMGCQIALDNFGSDMSAFMNLKHLPVDFVKID
GLLVRDIAQDQISRGIVESIHNVAKVMGMQTIAEHVESDVVLDAIANVGIDYAQGHYYDRAKPLLDVVSATRRSR
>Mature_554_residues
SDGINLLNHREGIDLILLDLCLSDAQGLETFYQVHQAFPAKPLIILSGNTDQSLVEEALQKGALDFLVKGQFDSTSLCRS
IQYALKQQHQSVEIGYRDLTLDALTRQLKIAQQELERLAEVDKVTRVFNRCRFDEVYLSEWNRLTREGSHLSLVLCEIDN
LQAYQEKYGAEVCDYCLQRVAEEIVKVARRPADCVARYSGRTFMILLPNTDLAGATYLAEAIRTGVRSVPFPPESLGVTN
SPITLSLGVACQIPRAKVAPSLLIEAVHQALHFAREQGKDRVQIHHTGCADPDLHSNQTLHWVSRLLKALDKDQFKLYAQ
PIYPLSRGQKSCSYEILLRLQDQPGIVCSPSLFLPMVEQYDFMTRIDRWVINNLLANLETMPALDELDARFFIHLSSATC
RNGELFEFLDKQFSHSNRHVQRFCFEVTENVALSHLTSTVELSRFLKSMGCQIALDNFGSDMSAFMNLKHLPVDFVKIDG
LLVRDIAQDQISRGIVESIHNVAKVMGMQTIAEHVESDVVLDAIANVGIDYAQGHYYDRAKPLLDVVSATRRSR

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=250, Percent_Identity=32.8, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI87082096, Length=238, Percent_Identity=33.6134453781513, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787541, Length=437, Percent_Identity=25.4004576659039, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790496, Length=238, Percent_Identity=31.0924369747899, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081921, Length=442, Percent_Identity=22.1719457013575, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI226510982, Length=282, Percent_Identity=27.6595744680851, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081980, Length=239, Percent_Identity=26.3598326359833, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788502, Length=247, Percent_Identity=22.2672064777328, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786507, Length=238, Percent_Identity=27.3109243697479, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788381, Length=445, Percent_Identity=21.7977528089888, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081743, Length=241, Percent_Identity=25.3112033195021, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081845, Length=252, Percent_Identity=25, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787055, Length=281, Percent_Identity=22.4199288256228, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 62611; Mature: 62480

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGINLLNHREGIDLILLDLCLSDAQGLETFYQVHQAFPAKPLIILSGNTDQSLVEEAL
CCCCCCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHH
QKGALDFLVKGQFDSTSLCRSIQYALKQQHQSVEIGYRDLTLDALTRQLKIAQQELERLA
HCCCHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVDKVTRVFNRCRFDEVYLSEWNRLTREGSHLSLVLCEIDNLQAYQEKYGAEVCDYCLQR
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCHHHHHHHHHH
VAEEIVKVARRPADCVARYSGRTFMILLPNTDLAGATYLAEAIRTGVRSVPFPPESLGVT
HHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCC
NSPITLSLGVACQIPRAKVAPSLLIEAVHQALHFAREQGKDRVQIHHTGCADPDLHSNQT
CCCEEEEECCEEECCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCH
LHWVSRLLKALDKDQFKLYAQPIYPLSRGQKSCSYEILLRLQDQPGIVCSPSLFLPMVEQ
HHHHHHHHHHHCCHHHEEEECCCCCHHCCCCCCCEEEEEEECCCCCEEECCHHHHHHHHH
YDFMTRIDRWVINNLLANLETMPALDELDARFFIHLSSATCRNGELFEFLDKQFSHSNRH
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHH
VQRFCFEVTENVALSHLTSTVELSRFLKSMGCQIALDNFGSDMSAFMNLKHLPVDFVKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHCHHCCCCCCEEEC
GLLVRDIAQDQISRGIVESIHNVAKVMGMQTIAEHVESDVVLDAIANVGIDYAQGHYYDR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHH
AKPLLDVVSATRRSR
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDGINLLNHREGIDLILLDLCLSDAQGLETFYQVHQAFPAKPLIILSGNTDQSLVEEAL
CCCCCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHH
QKGALDFLVKGQFDSTSLCRSIQYALKQQHQSVEIGYRDLTLDALTRQLKIAQQELERLA
HCCCHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVDKVTRVFNRCRFDEVYLSEWNRLTREGSHLSLVLCEIDNLQAYQEKYGAEVCDYCLQR
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCHHHHHHHHHH
VAEEIVKVARRPADCVARYSGRTFMILLPNTDLAGATYLAEAIRTGVRSVPFPPESLGVT
HHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCC
NSPITLSLGVACQIPRAKVAPSLLIEAVHQALHFAREQGKDRVQIHHTGCADPDLHSNQT
CCCEEEEECCEEECCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCH
LHWVSRLLKALDKDQFKLYAQPIYPLSRGQKSCSYEILLRLQDQPGIVCSPSLFLPMVEQ
HHHHHHHHHHHCCHHHEEEECCCCCHHCCCCCCCEEEEEEECCCCCEEECCHHHHHHHHH
YDFMTRIDRWVINNLLANLETMPALDELDARFFIHLSSATCRNGELFEFLDKQFSHSNRH
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHH
VQRFCFEVTENVALSHLTSTVELSRFLKSMGCQIALDNFGSDMSAFMNLKHLPVDFVKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHCHHCCCCCCEEEC
GLLVRDIAQDQISRGIVESIHNVAKVMGMQTIAEHVESDVVLDAIANVGIDYAQGHYYDR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHH
AKPLLDVVSATRRSR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]