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The map label for this gene is moaA

Identifier: 158338612

GI number: 158338612

Start: 5564173

End: 5565168

Strand: Direct

Name: moaA

Synonym: AM1_5516

Alternate gene names: 158338612

Gene position: 5564173-5565168 (Clockwise)

Preceding gene: 158338611

Following gene: 158338613

Centisome position: 85.55

GC content: 48.49

Gene sequence:

>996_bases
ATGAATGCAGTTGATTATCTTCGAATTAGCTTGATTGATCGCTGCAATTTTCGCTGTCAATATTGCATGCCCGACACCCA
GGACTTGCAATTTTTGCAGCCCACAGCCGTTCTGACAGCGACAGAACTATTGACTTTAATTCGGGACGTTTTTATCCCGT
TGGGATTTGCTCGCTTCCGCCTTACAGGTGGGGAACCGCTACTGAGGCCCGATATTTTAGATATTGTGCGCGGAGTTATC
GCATTGCCTCAGGTTAAAGATTTGGCGATGACGACAAATGCGTTTTTACTTGAGCCTCTAGCTCAGGATTTATTTGAGGC
TGGATTGCGTCGCATCAATATCAGCCTAGATTCCCTCGTGCCAGAGACCTTTGCCACTATCGTGGGTAGAGATTCACAGA
GACACAAACGCTGGAATAAGGTCTGGGCAGGCATCCAAAAAGCTTATCAAGTCGGGTTTAACCCTCTCAAACTCAATGTG
GTGATTATTCCTGGAGTAAATGATCATGAGGTGTTAGATCTGGCAGCGTTGACCATTGACCGCCAATGGCATGTGCGGTT
TATTGAGTTTATGCCGATTGGGAACGCGGATTTATTTTGCGATCGCGGCTGGATACCCTCAGAAGAACTCCGAACCCAAA
TTCGAGATCGATGGGGCCTCACAGACGGACAAGTCCAGGGCAATGGCCCAGCCGATGTGTTCCAAATCCCAGGAGCCCAG
GGCACATTAGGGTTTATCAGTCAGATGTCGGAATGCTTTTGCGATCGCTGTAATCGCATGCGATTATCGGCGGATGGGTG
GCTGCGGCCTTGTTTACTGAATGAAACGGGACAGTTAGATTTAAAGACGGCCTTGAGGGATGGCGTGTCTACAGACGAAT
TAAGAGATCGCGTCCGGCAGTTATTGGAACTCAAGCCAGAGATAAATTACAAGGAGCGACAATCTGGCACGGTGGGAGCA
AACTATAGCCGCACCATGTCTCAAATTGGCGGATAA

Upstream 100 bases:

>100_bases
GGTTTGGGGCGTTGTTGAGCGCCCCAATTTTTTTTGTCGATAACGTTAAAGTAGAAGACATCGCGGTTAGTTGTTCCTAG
CCCTATAAAAGGCTGATGTA

Downstream 100 bases:

>100_bases
CTCAAATGTACGCGCAACATACACTAGAAGAGGCGTACGTTGATGCAACGTATGTTTGTTGCCGACTACGGGTAAAGGTA
AAGTGTGAATTGTGCCAAAT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNAVDYLRISLIDRCNFRCQYCMPDTQDLQFLQPTAVLTATELLTLIRDVFIPLGFARFRLTGGEPLLRPDILDIVRGVI
ALPQVKDLAMTTNAFLLEPLAQDLFEAGLRRINISLDSLVPETFATIVGRDSQRHKRWNKVWAGIQKAYQVGFNPLKLNV
VIIPGVNDHEVLDLAALTIDRQWHVRFIEFMPIGNADLFCDRGWIPSEELRTQIRDRWGLTDGQVQGNGPADVFQIPGAQ
GTLGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTALRDGVSTDELRDRVRQLLELKPEINYKERQSGTVGA
NYSRTMSQIGG

Sequences:

>Translated_331_residues
MNAVDYLRISLIDRCNFRCQYCMPDTQDLQFLQPTAVLTATELLTLIRDVFIPLGFARFRLTGGEPLLRPDILDIVRGVI
ALPQVKDLAMTTNAFLLEPLAQDLFEAGLRRINISLDSLVPETFATIVGRDSQRHKRWNKVWAGIQKAYQVGFNPLKLNV
VIIPGVNDHEVLDLAALTIDRQWHVRFIEFMPIGNADLFCDRGWIPSEELRTQIRDRWGLTDGQVQGNGPADVFQIPGAQ
GTLGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTALRDGVSTDELRDRVRQLLELKPEINYKERQSGTVGA
NYSRTMSQIGG
>Mature_331_residues
MNAVDYLRISLIDRCNFRCQYCMPDTQDLQFLQPTAVLTATELLTLIRDVFIPLGFARFRLTGGEPLLRPDILDIVRGVI
ALPQVKDLAMTTNAFLLEPLAQDLFEAGLRRINISLDSLVPETFATIVGRDSQRHKRWNKVWAGIQKAYQVGFNPLKLNV
VIIPGVNDHEVLDLAALTIDRQWHVRFIEFMPIGNADLFCDRGWIPSEELRTQIRDRWGLTDGQVQGNGPADVFQIPGAQ
GTLGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTALRDGVSTDELRDRVRQLLELKPEINYKERQSGTVGA
NYSRTMSQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:
GO:0000166: Molybdenum cofactor biosynthesis protein A
GO:0003824: Molybdenum cofactor biosynthesis protein A
GO:0005525: Molybdenum cofactor biosynthesis protein A
GO:0006777: Molybdenum cofactor biosynthesis protein A
GO:0008152: Molybdenum cofactor biosynthesis protein A
GO:0019008: Molybdenum cofactor biosynthesis protein A
GO:0046872: Molybdenum cofactor biosynthesis protein A
GO:0051536: Molybdenum cofactor biosynthesis protein A
GO:0051539: Molybdenum cofactor biosynthesis protein A

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=332, Percent_Identity=41.2650602409639, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI22027536, Length=332, Percent_Identity=41.2650602409639, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI1786999, Length=326, Percent_Identity=34.3558282208589, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI32566331, Length=310, Percent_Identity=39.6774193548387, Blast_Score=213, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI32566329, Length=338, Percent_Identity=38.7573964497041, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17561210, Length=338, Percent_Identity=36.6863905325444, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI28574488, Length=332, Percent_Identity=38.5542168674699, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28574490, Length=306, Percent_Identity=39.5424836601307, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_ACAM1 (B0CDZ6)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519789.1
- ProteinModelPortal:   B0CDZ6
- SMR:   B0CDZ6
- GeneID:   5684307
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5516
- HOGENOM:   HBG529885
- OMA:   TEDPCFL
- ProtClustDB:   CLSK896062
- BioCyc:   AMAR329726:AM1_5516-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37436; Mature: 37436

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 8-8 BINDING 21-21 BINDING 60-60 BINDING 64-64 BINDING 91-91 BINDING 115-115 BINDING 157-157 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVDYLRISLIDRCNFRCQYCMPDTQDLQFLQPTAVLTATELLTLIRDVFIPLGFARFR
CCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEE
LTGGEPLLRPDILDIVRGVIALPQVKDLAMTTNAFLLEPLAQDLFEAGLRRINISLDSLV
ECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHH
PETFATIVGRDSQRHKRWNKVWAGIQKAYQVGFNPLKLNVVIIPGVNDHEVLDLAALTID
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHEEC
RQWHVRFIEFMPIGNADLFCDRGWIPSEELRTQIRDRWGLTDGQVQGNGPADVFQIPGAQ
CCEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCEEECCCCC
GTLGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTALRDGVSTDELRDRVRQ
CHHHHHHHHHHHHHHHHCCEEECCCCCCCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHH
LLELKPEINYKERQSGTVGANYSRTMSQIGG
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNAVDYLRISLIDRCNFRCQYCMPDTQDLQFLQPTAVLTATELLTLIRDVFIPLGFARFR
CCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEE
LTGGEPLLRPDILDIVRGVIALPQVKDLAMTTNAFLLEPLAQDLFEAGLRRINISLDSLV
ECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHH
PETFATIVGRDSQRHKRWNKVWAGIQKAYQVGFNPLKLNVVIIPGVNDHEVLDLAALTID
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHEEC
RQWHVRFIEFMPIGNADLFCDRGWIPSEELRTQIRDRWGLTDGQVQGNGPADVFQIPGAQ
CCEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCEEECCCCC
GTLGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTALRDGVSTDELRDRVRQ
CHHHHHHHHHHHHHHHHCCEEECCCCCCCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHH
LLELKPEINYKERQSGTVGANYSRTMSQIGG
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA